Effects of Cry1Ab Protein from Transgenic Bt Rice on the Activities of Relationship Enzymes and

Bioa
Author :ZhouYing
Tutor:WangZhi
School :Hunan Agricultural University
CLC :S476.2
TYPE :Masters thesis
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Year:2013
Abstract:
Our study focused on the enrichment amount of Cry1Ab protein in the Bt rice--Nilaparvata
lugens(Stal)--Pardosa pseudoannulata spiders, and investigated the relationship between the Bt
enrichment and the activity of the relative enzymes in these spiders. And our results showed:1. The
enrichment of Cry1Ab protein existed in the planthoppers fed with the Bt rice, and reached the peak at
the10th-15th day. The enrichment of Cry1Ab protein existed in the spiders which fed with
planthoppers eating Bt rice10th-15th day, and reached the peak at the10th day; besides, the Bt contents
in the3th instar of Pardosa Pseudoannulata was higher than that of5th instar.2. The CrylAb protein
existed in digestive system tissue, muscle tissue (appendages) and blood lymphocytes of adult spiders,
instead of nerve tissue; and the Cry1Ab protein in mitochondria and microsome of spiders were higher
than that of cell debris or cytoplasmic extracts; CrylAb protein was also detected in mitochondria and
microsome, cell debris, cytoplasmic extracts in the3th instar and5th instar of Pardosa
Pseudoannulata.3. Only AchE activities in muscle tissue and nerve tissue of experimental male
spiders, muscle tissue of experimental female spiders and SOD activities in muscle tissue of
experimental male5th spiders were significant higher than the control groups (p<0.05); in different
maturity, AchE activities in digestive system of experimental3th instar spiders, POD activities in
muscle tissue of experimental5th instar spiders were significantly higher than the control (p<0.05).
However, activities of other enzymes in all tissue had no significant difference compared with the
control groups (p<0.05). It showed that the enrichment of CrylAb protein had little effects on
enzymes activities in spiders.4. Only AchE activities in mitochondria and microsome of digestive
system tissue, nerve tissue, blood lymphocytes of experimental male spiders, in cytoplasmic extracts
of muscle tissue, blood lymphocytes of experimental male spiders, in cytoplasmic extracts of muscle
tissue, blood lymphocytes of experimental female spiders had significant difference with the control
groups (p<0.05); SOD activities in cell debris of muscle tissue of experimental male spiders, CAT
activities in mitochondria and microsome of digestive system of experimental female and male
spiders, POD activities in mitochondria and microsome of blood lymphocytes of experimental male
spiders and in cytoplasmic extracts of muscle tissue of experimental female spiders had significant
difference with the control groups (p<0.05); activities of others enzymes in all subcellular structure
had no significance with the control groups. While in different instar spiders, POD activities in
cytoplasmic extracts of digestive system and muscle tissue of experimental3th instar spiders were
significantly higher than the control groups (p<0.05). POD activities in cytoplasmic extracts of
muscle tissue of experimental5th instar spiders were significantly higher than the control groups
(p<0.01); GSH-Px activities in cytoplasmic extracts of blood lymphocytes of experimental3th instar
spiders were significantly higher than the control groups (p<0.05). AchE activities in mitochondria
and microsome of muscle tissue of experimental3th instar spiders were significantly lower than the
control groups (p<0.05); but in nerve tissue of experimental3th instar spiders were significantly
higher than the control groups (p<0.05). AchE activities in cytoplasmic extracts of digestive system
of experimental3th instar spiders were significantly higher than the control groups (p<0.05).
However, activities of other enzymes in all subcellular structure had no significance with the control
groups.In our results, CrylAb protein could bioaccumulate in muscle tissue from digestive system
tissue to blood lymphocytes. All the Bt enrichment has some effects on the enzyme activities, but
whether it affects the spiders safety needs further study.