・DNA Step Ladders

・OneSTEP Ladders
・Ladders for 10minute separation
・Wide Range Ladders
・Markers ＆ OneSTEP Markers Size St
・RNA Si Standard
d Markers
・DGGE Markers ・RNA Step Ladder
・PCR Marker Makers ・Loading Buffers

Molecular-Weight
Markers
・Easy SeparatorTM
・Negative Gel Stain MS Kit
・SuperSepTM Ace
・Prestain markers ・SuperSepTM DNA
・Western marker
・Biotinylated marker
・Markers for CBB dyeing
 DNA Ladder
DNA Ladder

DNA Step Ladder (1~6) Ready With dye and glycerol already
to added, it is ready to be applied to
use the agarose gel.
for low-molecular-range analysis.

10 bp DNA Step 25 bp DNA Step 25 bp DNA Step 50 bp DNA Step

Ladder (10-100 bp) Ladder (25-300 bp) Ladder (25-500 bp) Ladder (50-800 bp)

−20℃ （bp） −20℃ （bp） −20℃ （bp）（ng） −20℃ （bp）

100 300 500 60
800
275 400 60 700
90
6xBlue/ 250 300 60 6xBlue/ 600
80 6x
250 60 500
Orange Loading 225 Orange
Loading 70 Dye 200 200 120 Loading
Dye Dye 400
60 175 175 80
50 150 300
150 80
40 125 125 80 200
Loading: Loading: 100 Loading: Loading:
1µL/lane 30 5µL/lane 5µL/lane 100 120 5µL/lane
75 150
20 75 80

3.5% Agarose
50 100
2% Agarose

2% Agarose
1% Agarose

10 50 80
25 25 80 50

1 2 3 4

  
OneSTEP Ladder (7~12)
for simultaneous measurement of DNA size and amount.

DNA Step Ladder 100 bp DNA Step Ladder OneSTEP Ladder 50 OneSTEP Ladder 100
(10-300 bp) (0.1-1.5 kbp) (0.05-2 kbp) (0.1-2 kbp)
（bp） （bp） （bp）（ng/5µL） （bp）（ng/5µL）
Ready −20℃ Ready 2,000 100 Ready
to 300 1,500 to to 2,000 100
use 1,000 use 1,500 75 use 1,800 90
200 1,000 50 1,600 80
900 900 45
−20℃ 150 800 40 1,400 70
6x 800
Loading 700 35 1,200 60
100 Dye 717 1,000 100
75 600 30
600 500 75 900 45
6x 50 400 22.5 800 40
Loading
Dye 35 500 450 20 700 35
25 400 350 17.5 600 30
20 300 15 500 50
5% Agarose 21

3% Agarose 21

3% Agarose 21

250 37.5
15 286 200 10 400 20
2% Agarose

10 150 7.5 300 15

200 100 10 200 10
100 10

100 50 100

5 Loading:
5-10µL/lane
6 Loading:
5µL/lane
7 Concentration:
0.69µg/5µL
8 Concentration:
0.755µg/5µL

OneSTEP Ladder 500 OneSTEP Ladder 1 kb OneSTEP Ladder Gene Ladder 100
(0.5-5 kbp) (1-10 kbp) Supercoiled Plasmid (0.1-2 kbp)

Ready （bp） Ready （kbp） Ready （kbp） Ready （bp）

（ng/5µL）
to 5,000 to to to
use use use use
4,500 10.0 2,000 100
4,000 9.0 10.0
8.0 −20℃ 1,500 75
3,500 9.0
3,000 7.0 8.0 1,000 50
2,500 6.0 7.0 900 45
2,000 5.0 6.0
4.0 800 40
3.0 5.0 700 35
1,500 600 30
2.0 4.0
500 75
1% Agarose S

2% Agarose S
1% Agarose S

1% Agarose S

1,000 3.0 400 40

300 30
200 20
500 1.0 100 100

9 each fragment:
50ng/5µL 10 each fragment:
50ng/5µL 11 each fragment:
30ng/5µL 12 Concentration:
0.64µg/5µL

2
 Ladder for 10minute separation (13~18) −20℃
      : Store at ｰ20℃
NO DISPLAY: Store at 2∼10℃

DNA Step Ladder DNA Step Ladder DNA Step Ladder DNA Step Ladder Gene Ladder Fast 1
(10-200 bp) (50-1,500 bp) (100-5,000 bp) (500-10,000bp) (4 bands:0.1-2 kbp)

（bp） （bp） （bp）

（ng/5µL）

1% Agarose S
Ready Ready （bp） Ready （bp） Ready Ready
to to to to to
use use use use use
10,000
−20℃ −20℃ −20℃ 5,000 −20℃ 4,000 2,000 25
200 2,000 2,000 1,000 25
100 1,500 500 25
6x 6x 6x 6x 1,000 100 25
Loading 50 Loading
850 Loading 850 Loading
Dye Dye Dye Dye
20 500 17 Concentration:
4% Agarose 21

400 400
10 0.1µg/5µL

2% Agarose
2% Agarose

2% Agarose
200
100 Gene Ladder Fast 2
50 (6 bands:0.1-10 kbp)

1% Agarose S
Ready （bp）
（ng/5µL）
to
use
Loading: Loading: Loading: Loading: 10,000 25
20µL/lane 20µL/lane 20µL/lane 20µL/lane 4,000 25
2,000 25
1,000 25
500 25
100 25
13 14 15 16 18
Concentration:
0.15µg/5µL

Number Product name Code No. Package Size Note

1 10 bp DNA Step Ladder (10-100 bp) 040-28721 32.5 μg (50 μL)
2 25 bp DNA Step Ladder (25-300 bp) 292-69001 100 μg
3 25 bp DNA Step Ladder (25-500 bp) 044-30711 500 μL
4 50 bp DNA Step Ladder (50-800 bp) 544-02311 85 μg (250 μL)
5 DNA Step Ladder（10-300 bp） 298-66301 50 μg
6 100 bp DNA Step Ladder（0.1-1.5 kbp） 296-68801 30 μg
319-06044 25 μL
7 OneSTEP Ladder 50（0.05-2.0 kbp） 315-06041 500 μL
311-06043 500 μL ×2
317-05244 25 μL
8 OneSTEP Ladder 100（0.1-2 kbp） 313-05241 500 μL
319-05243 500 μL ×2
317-05364 25 μL
9 OneSTEP Ladder 500 (0.5-5 kbp) 313-05361 500 μL
319-05363 500 μL × 3
314-05374 25 μL
10 OneSTEP Ladder 1 kb（1-10 kbp） 310-05371 500 μL
316-05373 500 μL × 3
11 OneSTEP Ladder Supercoiled Plasmid 317-05381 500 μL
12 Gene Ladder 100（0.1-2 kbp） 316-06951 500 μL
13 DNA Step Ladder（10-200 bp）
, Fast Separation Type 292-66201 10 μg（0.5mL）× 2
14 DNA Step Ladder（50-1,500 bp）
, Fast Separation Type 290-61001 10 μg（0.5mL）× 2
15 DNA Step Ladder（100-5,000 bp）
, Fast Separation Type 296-61101 10 μg（0.5mL）× 2
16 DNA Step Ladder（500-10,000 bp）
, Fast Separation Type 292-61201 10 μg（0.5mL）× 2
17 Gene Ladder Fast 1
（4 bands:0.1-2 kbp） 317-06981 500 μＬ× 2
18 Gene Ladder Fast 2（6 bands:0.1-10 kbp） 314-06991 500 μＬ× 2

: Nippon Gene s products

3
 DNA Ladder
DNA Ladder

der (19~22)
Wide Range Ladder
for wide-range and simultaneous analysis
l i off b
both
th DNA size
i and
d amount.
t

DNA Step Ladder Mix Smart Ladder Gene Ladder Wide 1 Gene Ladder Wide 2
(0.08-10 kbp) (0.2-10 kbp) (0.1-20 kbp) (0.1-20 kbp)

−20℃ （bp）（ng/20µL） Ready （bp） （ng/5µL） Ready （bp）

（ng/5µL） Ready （bp）
（ng/5µL）
10,000 200 to 10,000 100 to to
use use use
8,000 160 8,000 80 20,000 100 20,000 100
6,000 120
5,000 100 −20℃ 6,000 60 10,000 50 10,000 50
6x 4,000 80 5,000 50 7,000 35 7,000 35
Loading 3,000 60
Dye 4,000 40 5,000 75 5,000 75
2,500 50 4,000 40 4,000 40
2,000 40 3,000 30
2,500 25 3,000 30 3,000 30
1,500 32 2,000 20
1,500 15 2,000 100 2,000 100
1,030 200 1,000 100 1,500 30 1,500 30
900 180 1,300 26 1,000 50
1% Agarose S

800 80
800 160 1,000 100 900 45
600 60 800 40
700 140
600 120 400 40 700 35 700 35
200 20 500 75 600 30

1% Agarose
1% Agarose
500 200 500 75
400 80 400 40
1% Agarose

300 30 400 40
300 60 200 20 200 20
200 40 100 100 100 100
19 100
80
20
16
20 21 22

Ready
Marker −20℃ & the OneSTEP Marker to
use (23~33)
DNA molec
molecular-weight
lar eight markers that set si
size
e standards for linear do
double-strand
ble strand DNA
DNA. EEach One-Step Marker
comes with pre-added dye and glycerol, so they can be applied directly to the agarose gel.

Marker 1 Marker 2 Marker 3 Marker 4

(λ/Hind Ⅲ) (λ/Hind Ⅲ・EcoRⅠ) (λ/Hind Ⅲ+λ/EcoRⅠ) (φX174/Hae Ⅲ)

（kbp） （kbp） （kbp） （bp）

23.13 21.23 23.13 1,353
1,078
9.42 5.15 21.23 872
6.56 9.42
4.98 7.42 603
4.36 4.27 6.56
5.81
3.52 5.65
2.02 4.88 310
2.32 4.36
2.02 1.91 3.52 281
3% Agarose 21

2.32 271
1% Agarose S
1% Agarose S
1% Agarose S

1.58
2.02 234
1.38
194
0.95
118
23 24 0.83 25 26 72

Marker 5 Marker 6 Marker 7GT Marker 8GT

(φX174/Hinc Ⅱ) (λ/StyⅠ) (T4 GT7 + T4 GT7/BglⅠ) (T4 GT7 + T4 GT7/Bgl Ⅱ)

（bp） （kbp） （kbp） （kbp）

1,057 19.33 165.65 165.65
770 50.31 56.74
7.74 41.77
612 6.22
495 23.45
392 4.26 17.82
21.10
345 3.47 17.70 17.70
341 15.71
2.69 14.28
335
297 10.65
10.06
0.3% Agarose H

0.3% Agarose H

291 1.88
3% Agarose 21

210 8.03
1% Agaose S

162 1.49
5.59
0.93 5.51
27 79 28 29 30
4
 Marker 9 Marker 10 Marker 11
(φX174/HinfⅠ) (pBR322/MspⅠ) (pUC19/MspⅠ)

（bp） （bp） （bp）

726 622
713 527
404 501
553
500 307 489
242 404 Storage conditions
427
417 238 331 DNA MW Marker： 
413 217 242
201 −20℃
311 190 190
249 147 OneSTEP Marker：
180
200 160 111  Store at 2∼10℃
151

3% Agarose 21
160
3% Agarose 21

3% Agarose 21
140 110
147
118 147
100 123
82 110
66 90
31 66
48 32 76
67 33
Number Product name Code No. Package Size Note
19 DNA Step Ladder Mix（0.08-10 kbp） 292-68401 1 mL
313-03943 25 μL
20 Smart Ladder (0.2-10 kbp)
317-03941 500 μL
21 Gene Ladder Wide 1（0.1-20 kbp） 313-06961 500 μL × 2
22 Gene Ladder Wide 2（0.1-20 kbp） 310-06971 500 μL × 2
310-05251 1500 μL
OneSTEP Marker 1（lambda/ d Ⅲ digest）
314-05254 1500 μL × 5
23
316-00454 120 μg
Marker 1（lambda/ d Ⅲ digest）
312-00456 120 μg × 5
OneSTEP Marker 2 317-05261 1500 μL
（lambda/ dⅢ+ R I double digest） 311-05264 1500 μL × 5
24
Marker 2 319-00564 80 μg
（lambda/ dⅢ+ R I double digest） 315-00566 80 μg × 5
OneSTEP Marker 3 314-05271 1500 μL
（lambda/ d Ⅲ + lambda/ R I digest mixture） 310-05273 1500 μL × 5
25
Marker 3 316-00574 80 μg
（lambda/ d Ⅲ + lambda/ R I digest mixture） 312-00576 80 μg × 5
318-05791 375 μL
OneSTEP Marker 4（phiX174/ Ⅲ digest）
314-05793 375 μL × 5
26
315-00664 15 μg
Marker 4（phiX174/ Ⅲ digest）
311-00666 15 μg × 5
311-05801 375 μL
OneSTEP Marker 5（phiX174/ c Ⅱ digest）
317-05803 375 μL × 5
27
312-00674 15 μg
Marker 5（phiX174/ c Ⅱ digest）
318-00676 15 μg × 5
311-05281 1500 μL
OneSTEP Marker 6（lambda/ Ⅰdigest）
317-05283 1500 μL × 5
28
313-00964 80 μg
Marker 6（lambda/ Ⅰdigest）
319-00966 80 μg × 5
315-03981 10 μg
29 Marker 7 GT
311-03983 10 μg × 5
312-03991 10 μg
30 Marker 8 GT
318-03993 10 μg × 5
318-05811 375 μL
OneSTEP Marker 9（phiX174/ fⅠdigest）
314-05813 375 μL × 5
31
314-01491 15 μg
Marker 9（phiX174/ fⅠdigest）
310-01493 15 μg × 5
315-05821 375 μL
OneSTEP Marker 10（pBR322/ Ⅰdigest）
311-05823 375 μL × 5
32
317-01501 15 μg
Marker 10（pBR322/ Ⅰdigest）
313-01503 15 μg × 5
312-05831 375 μL
OneSTEP Marker 11（pUC19/ Ⅰdigest）
318-05833 375 μL × 5
33
319-03261 15 μg
Marker 11（pUC19/ Ⅰdigest）
315-03263 15 μg × 5

: Nippon Gene s products

5
 DNA Ladder
DNA Ladder

DGGE Marker (34~38

4~38)
DNA markers for DGGE (dena
(denaturing
aturing grad
gradient gel electrophoresis)
electrophoresis). A CG clamp is a
added at the end of one side of
each fragment to enable separation / inspection by DGGE.

DGGE MarkerⅠ DGGE Marker Ⅱ DGGE Marker Ⅲ DGGE Marker Ⅳ DGGE Marker Ⅴ
(5 fragments) (10 fragments) (10 fragments) (8 fragments) (9 fragments)

Denaturant Denaturant Denaturant Denaturant Denaturant

35% 35% 50% 20% 20%
1 1 1
−20℃ −20℃ 2 −20℃ −20℃ −20℃
2 2
3 1
4 2 3
3 1
3 5 2 4
4 3 5
6 5 4 6
4 7 6 5
7 7
8 8 6 8
7 9
8% Acrylamide

8% Acrylamide

7% Acrylamide

6% Acrylamide
6% Acrylamide
5 9 9
8
10 10

34 65% 35 65% 36 70% 37 45% 38 50%

No. of fragments (Conc.) 5 (50ng/5µL) 10 (100ng/5µL) 10 (100ng/5µL) 8 (80ng/5µL) 9 (50ng/5µL)
Application For bacterial 16S rDNA For bacterial 16S rDNA For bacterial 16S rDNA For fungal bacteria16S rDNA For fungal bacteria 16S rDNA
Constant voltage 120V 120V 50V 50V 75V
Electrophoresis time, Temp. 8 hours, 60℃ 8 hours, 60℃ 18 hours, 58℃ 20 hours, 60℃ 16 hours, 60℃

PCR Marker Maker (39~42) −20℃

for preparing DNA size markers

markers. Consisting of a mixt
mixture of template DNA, primer, and reaction buffer, the reagent
initiates a PCR with the simple addition of a Tag DNA polymerase “Gene Tag.” It can also be used as a positive
control while the PCR is under way.
M 39 40 41 42 43
（bp） （bp）
1057 M：Marker 5（φX174/ c Ⅱ） 0.5 µg
770 39：100L（101 bp, 200 bp, 299 bp, 398 bp, 502 bp） 3 µL
1005
612 40：100H（601 bp, 702 bp, 800 bp, 906 bp, 1,005 bp） 3 µL
905
495 41：200U（101 bp, 299 bp, 502 bp, 702 bp, 906 bp） 3 µL
800
392 42：200E（200bp, 398 bp, 601 bp, 805 bp, 1,005 bp） 3 µL
702
345 3 µL each
601 43：Mixture of products from a 100L & 100H PCR
341
502
335
398
297
299
291
200
210
101
162
79

Number Product name Code No. Package Size Note

318-06411 30 μL（for 6 applications）
34 DGGE MarkerⅠ(5 fragments) 314-06413 150 μL（for 30 applications）
312-06414 150 μL × 5（for 150 applications）
311-06401 30 μL（for 6 applications）
35 DGGE Marker Ⅱ (10 fragments) 317-06403 150 μL（for 30 applications）
315-06404 150 μL × 5（for 150 applications）
315-06921 30 μL（for 6 applications）
36 DGGE Marker Ⅲ (10 fragments) 311-06923 150 μL（for 30 applications）
319-06924 150 μL × 5（for 150 applications）
312-06931 30 μL（for 6 applications）
37 DGGE Marker Ⅳ (8 fragments) 318-06933 150 μL（for 30 applications）
316-06934 150 μL × 5（for 150 applications）
319-06941 30 μL（for 6 applications）
38 DGGE Marker Ⅴ (9 fragments) 315-06943 150 μL（for 30 applications）
313-06944 150 μL × 5（for 150 applications）
PCR Marker Maker 100L 317-03061 For 10 applications
39
PCR Marker Maker 100L, Gene attached 314-03071 For 10 applications
PCR Marker Maker 100H 311-03081 For 10 applications
40
PCR Marker Maker 100H, Gene attached 318-03091 For 10 applications
PCR Marker Maker 200U 318-03111 For 10 applications
41
PCR Marker Maker 200U, Gene attached 315-03121 For 10 applications
PCR Marker Maker 200E 312-03131 For 10 applications
42
PCR Marker Maker 200E, Gene attached 319-03141 For 10 applications

: Nippon Gene s products

6
 RNA Ladder

RNA Ladder
RNA Size Standard Marker (1~4)
RNA molecular-weight markers prepared by in vitro transcription.

RNA Size Standard RNA Size Standard RNA Size Standard RNA Ladder
Marker Ⅱ Marker Ⅲ (0.1-1 kb) Marker Ⅳ (0.28-6.58 kb) (0.125-6 kb)

−70℃ （k base） −70℃ （base） −80℃ （base） −70℃ （base）

9
1,000 6,583
750 6,000
4,981 4,000
6 500

1% formaldehyde Agarose
5 400 3,638 3,000
2,000
4 300 2,604
1,500
1,908 1,000

1% formaldehyde Agarose
1% formaldehyde Agarose

3
500
5% polyacrylamide-urea

2.5 200 1,383

250
2 955 125
1.5
623
1
281
100
1 0.5 2 3 4

RNA Step Ladder (0.1–1 kb) (5) Loading Buffer （Store at room temp.）

an RNA molecular-weight
l l i ht marker
k can b be di
directly
tl applied
li d A sample can be easily applied during DNA gel
to native gel, denatured gel, or polyacrylamide gel. electrophoresis to effectively predict the migration
point.
a b c d e f a b c d e f
RNA Step Ladder (0.1-1 kb)

Xylene Cyanol FF
−80℃ （base） （base）

Bromophenol Blue
2X 1,000 1,000
Loading 800 800
Dye Orange G
600 600
2% Formaldehyde Agarose

400 400
300 a Marker 4 6 × Loading Buffer Triple Dye
300 b (φX173/Hae Ⅲ) 6 × Loading Buffer Orange G
2% Native Agarose

200 200 c Product PCR 6 × Loading Buffer Triple Dye

d (299 bp) 50 ng 6 × Loading Buffer Orange G
100 100 e
TE Buffer
6 × Loading Buffer Triple Dye
f 6 × Loading Buffer Orange G
Orange G Bromophenol Blue Xylene Cyanol FF

5 1 % agarose S
2 % agarose S
100 bp (approx.) 900 bp (approx.) 5,000 bp (approx.)
30 bp (approx.) 300 bp (approx.) 1,500 bp (approx.)
3 % agarose 21 10 bp (approx.) 100 bp (approx.) 500 bp (approx.)

Number Product name Code No. Package Size Note

1 RNA Size Standard Marker Ⅱ（0.5-9 kb） 542-00651 50 μg
2 RNA Size Standard Marker Ⅲ（0.1-1 kb） 545-01621 50 μg
3 RNA Size Standard Marker Ⅳ（0.28-6.58 kb） 188-01831 50 μL
311-06261 25 μL
4 RNA Ladder（0.125-6 kb）
317-06263 25 μL × 2
5 RNA Step Ladder（0.1-1 kb） 298-61301 40 μL × 5
− Loading Buffer 313-90111 10 mL
− 6 × Loading Buffer OrangeG 317-90251 1 mL × 3
− 6 × Loading Buffer Double Dye 313-90351 1 mL × 3
− 6 × Loading Buffer Triple Dye 314-90261 1 mL × 3

: Nippon Gene s products

7
 Protein Size Marker
Protein Size Marker

Prestained Marker (1, 2) : With dye combined in its recombinant protein content.
Western Marker (3) : Contains protein G with the ability to combine with the Fc region
of immunoglobulin. Reacts with both primary and secondary
antibodies in a western blot.

Biotinylated Marker (4) : Contains biotinylated protein. Capable of both chromogenetic and
photogenetic detection by reacting with avidin HRP or avidin AP.

−20℃ （kDa） −20℃ （kDa） −20℃ （kDa） （kDa）

240
140 140 150 79
120
100 100
100
70 80
70 42
50 60
50
50
40 42
35 31
35
30
25
20 25 21
20
15
14
15 16
7
1 Prestained 2 PrestainedⅡ 3 Western 4 Biotinylated

Marker for CBB Dyeing (5~8)

Optimized for CBB dyeing. Obtains sharp, uniform bands because of a reduction alkyl process.
（kDa） （kDa） （kDa） （kDa）

200 180
116
116 97
79
79 79

42
42
42 42
30 30
30 30

20 20
20 17
14
17 14
6.5 6.5
3.5

5 For low molecular

range 6 For middle molecular
range 7 For high molecular
range 8 Wide range

−20℃
      : Store at ｰ20℃
NO DISPLAY: Store at 2∼10℃

Number Product name Wako Cat. No. Package Size

1 WIDE-VIEW TM Prestained Protein Size Marker 230-02221 500 μL
2 WIDE-VIEW TM Prestained Protein Size Marker 2 239-02291 500 μL
3 WIDE-VIEW TM Western Size Marker 233-02211 250 μL
4 WIDE-VIEW TM Biotinylated Size Marker 233-02331 1.5 mL
5 Molecular Weight Marker, Low Range 294-63101 1 mL
6 Molecular Weight Marker, Middle Range 131-14511 1 mL
7 Molecular Weight Marker, High Range 134-14501 1 mL
8 Molecular Weight Marker, Wide Range 296-63301 1 mL

8
 Electrophoresis-related Products

Electrophoresis-related Products
Easy SeparatorTM (Wako Catalog No.058-07681 (1 set ) )
Easy SeparatorTM is an electrophoresis tank specially designed for “SuperSep” products to enable easy setting
and removal of the gel plate.

Features
1 Easy to operate.
2 Requires only a small amount (about 250-350ml) of
running buffer for electrophoresis.
3 Allows the gel plate to be removed without removing the
running buffer after electrophoresis has been completed.

Easy Set Easy Remove

①Insert a gel. ②Turn the knobs ③Setting is ①Remove the cover ②Turn the knobs ③Remove the gel
toward you. completed. after electrophoresis away from you. without removing
has been completed. the running buffer.

Negative Gel Stain MS Kit （Store at room temperature）

Protein bands separated by SDS/polyacrylamide gel electrophoresis (SDS-PAGE) can be visualized as stable,
clear and transparent bands against the background of milky white gel stained by negative gel stain containing
our improved imidazol derivative reagent in as little as 10 minutes. The staining technique is useful to obtain the
clear and sensitive resolution pattern of the gel before immunoblotting as well as to excise and purify the band
of interest from the gel without significant deterioration of amino acid residues for the subsequent studies of
protein such as sequencing and mass analysis of peptide.

Procedure Featureas
SDS-PAGE gel 1 Detectable limit of protein: 1 ng
Staining Sol. A 2 Sensitive and Simple procedure with 2-step staining in
Shake 5 10 min. 5~10 minutes
Wash 5 10 sec. x 3
3 Optimized the staining intensity by either de-staining the
Staining Sol. B excess stain or re-staining following de-stain of the gel.
Shake 10 60 sec.
Wash 1 min. x 3 4 Applicable to proteome analysis by mass spectrometry
and sequencing
Check staining intensity
of the gel against a sheet
Mass Spectrometry of black paper MALDI-TOF/MS analysis
rat muscle glycogen phosphorylase (EC2.4.1.1)

SDS-PAGE analysis
relative abundance

1 2 3

a Figure 1.
b SDS-PAGE analysis of proteins using
Negative Gel Stain MS Kit
c MALDI-TOF/MS of
a : rat phosphorylase (97k);
b : bovine serum albumin (68k);
d c : hen egg white ovalbumin (45k);
d : bovine carbonic anhydrase (31k).
Lane 1: 50 ng; Lane 2: 100 ng; Lane 3: 150 ng
Figure 2.
1000 1100 1200 1300 1400 1500 1600 1700 1800 1900 2000 rat phosphorylase
m/z
The band was excised and treated with trypsin. Following the in-gel digestion and
preparation, the sample was analyzed on MALDI-TOF mass spectrometer. (These data
were provided by Dr. Y. Wada at Osaka Medical Center, Japan.)

Product name Wako Cat. No. Package Size

Negative Gel Stain MS Kit
Kit Contents: 293-57701 20 tests
1) Staining Solution A (1 × 500 mL); 2) Staining Solution B (1 × 500 mL); 3)De-staining Solution (1 × 500 mL)

9
 Electrophoresis-related Products
Electrophoresis-related Products

SuperSepTM Ace
SuperSep™ Ace is a polyacryla
polyacrylamide
amide gel of the highest quality
quality, 13 well 17 well
designed to resolve the problems of existing products (SuperSep™ and
SuperSep™ HG). Tris-glycine buffer is used in the gel buffer, similar to
existing products, but because its pH is neutralized, the gel’s chemical
storage stability is improved. Physical storage stability is also improved
by using vacuum packaging. And because it does not contain SDS, it
can be used in SDS-PAGE and Native-PAGE by changing the running
t
buffer. enien
Conv er for
stick el
g n!
Features n t if icatio
ide
1 Good separation and uniform bands
2 Can be used with a multi-channel pipette.
3 Reasonably priced and capable of long-term storage (for 6 months)
4 Box sticker allows easy identification of type of gel.
5 Comes in 13-well and 17-well types.

oved
Impr ation
r
Product specifications sepa center!
th e
Plate size 100 × 100 × 3（mm） near SuperSepTM SuperSepTM
Ace
Gel size 90 × 85 × 1（mm） （kDa）
Number of wells 13 wells 17 wells 200
30 μL 25 μL 150
Well volume
100
Total amount of protein＊ (/well) 3.3 ∼ 6.5 μg 1.3 ∼ 3.9 μg 75
＊Indicates total amount of protein that can be clearly separated.

50

rm
Unifo d!
37
① SuperSepTM: 10%
ban 0 1.5 3 4.5 6 7.5 9 10.5 12 (hours)

25
TM
②SuperSep Ace: 10% 20

（Store at 2∼10℃）

Product name Wako Cat. No. Package Size

SuperSepTMAce, 6%, 13well 195-15171 10 ea.
SuperSepTMAce, 6%, 17well 192-15181 10 ea.
SuperSepTMAce, 7.5%, 13well 198-14941 10 ea.
SuperSepTMAce, 7.5%, 17well 191-14931 10 ea.
SuperSepTMAce, 10%, 13well 195-14951 10 ea.
SuperSepTMAce, 10%, 17well 192-14961 10 ea.
SuperSepTMAce, 12.5%, 13well 199-14971 10 ea.
SuperSepTMAce, 12.5%, 17well 196-14981 10 ea.
SuperSepTMAce, 15%, 13well 193-14991 10 ea.
SuperSepTMAce, 15%, 17well 190-15001 10 ea.
SuperSepTMAce, 5-12%, 13well 199-15191 10 ea.
SuperSepTMAce, 5-12%, 17well 192-15201 10 ea.
SuperSepTMAce, 5-20%, 13well 197-15011 10 ea.
SuperSepTMAce, 5-20%, 17well 194-15021 10 ea.
SuperSepTMAce, 10-20%, 13well 191-15031 10 ea.
SuperSepTMAce, 10-20%, 17well 198-15041 10 ea.
SuperSepTMAce, 15-20%, 13well（Tricine Gel） 198-15301 10 ea.

10
 SuperSepTM DNA (15%, 17 wells) (Wako Catalog No.190-15481 (10 ea.) ) （Store at 2∼10℃）

Polyacrylamide gel is used in the separation of low-molecular-weight DNA because it has a finer gel mesh
structure than agarose. However, the DNA size will vary widely depending on the acrylamide concentration and
the degree of cross-linkage of the gel. In SuperSepTM DNA, the acrylamide concentration and the degree of
cross-linkage of the gel have been optimized for the separation of low-molecular-weight DNA. This product is
therefore effective for the separation of samples with a DNA size from 20-200 bp.

Features Product specifications

1 The acrylamide concentration, degree of Plate size 100 × 100 × 3（mm）
cross-linkage, and number of wells are optimized for Gel size 90 × 85 × 1（mm）
the electrophoresis of low-molecular-weight DNA. Number of wells 17 wells
2 Cleaner separation image than that obtained with Well volume 25 μL
agarose gel.
3 Ideal for 20-200 bp separation.

（bp） （bp） （bp） （bp）

100
90
80
200
70 200
60 150 150
100
50
90 100
40 100
80
30 70 75 75
20 60 50
50 50
40 25

30
25
20

1% Agarose SuperSepTM DNA 2.5% Agarose SuperSepTM DNA

（15%, 17 well） （15%, 17 well）

Example of electrophoresis
20-200 bp DNA was clearly separated by using SuperSepTM DNA and Tris-glycine buffer. PCR products under 200
bp can be used for separation from gel, especially when cloning small RNA. 30-200 bp DNA was clearly
separated with TBE buffer.

① With Tris-Glycine running buffer ② With TBE running buffer

（bp） （bp） （bp） （bp）

200
150 200
100 150
100
50
50
30
25 30
20 20 25

EtBr staining EtBr staining

Staining Reagent
Product name Wako Cat. No. Package Size
EtBr Solution 315-90051 10 mL

11
Selection Guide
【How to read the chart】 【Display codes】
● 1 , p1 NO DISPLAY Store at 2∼10℃ −80℃ Store at or below ｰ80℃
Number Reference page −20℃ Store at or below ｰ20℃ −70℃ Store at or below ｰ70℃

DNA Ladders
10 bp 100 bp 1 kbp 10 kbp 100 kbp 200 kbp

●
1 , p2
●
2 , p2
●
3 , p2
●
4 , p2
●
5 , p2
●
6 , p2
●7 , p2
●8 , p2
●
9 , p2
●
10 , p2
●
11 , p2
●
12 , p2
●
13 , p3
●
14 , p3
●
15 , p3
●
16 , p3
●
17 , p3
●
18 , p3
●
19 , p4
●
20 , p4
●
21 , p4
●
22 , p4
●
23 , p4
●
24 , p4
●
25 , p4
●26 , p4
●
27 , p4
●
28 , p4
●
29 , p4
●30 , p4
●
31 , p5
●
32 , p5
●
33 , p5
●
39 , p6
●
40 , p6
●
41 , p6
●
42 , p6

10 bp 100 bp 1 kbp 10 kbp 100 kbp 200 kbp

RNA Ladders
10 b 100 b 1000 b 10000 b

●
1 , p7
●
2 , p7
●
3 , p7
●
4 , p7
●
5 , p7

10 b 100 b 1000 b 10000 b

Protein Size Markers

10 kDa 100 kDa 1000 kDa

●
1 , p8
●
2 , p8
●
3 , p8
●
4 , p8
●
5 , p8
●
6 , p8
●7 , p8
●
8 , p8

10 kDa 100 kDa 1000 kDa

・This brochure may contain products that cannot be exported to your country due to regulations.
・Listed products are intended for laboratory research use only, and not to be used for drug, food or human use.

co.jp

08X05開01

09Y3IBF