Code No: 45056 R07 Set No - 1

III B.Tech I Semester Regular Examinations,Nov/Dec 2009

PLANT BIOTECHNOLOGY
Bio-Technology
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
?????

1. What are Protoplasts? Discuss different methods for isolation & purification of
Protoplast. [16]

2. Write a short note on permeabilization of cells. Explain how it can be used for
secondary metabolites production? List out some of the advantages. [16]

3. Discuss the various strategies involved in the production of rare fatty acids in plants.
Explain with examples. [16]

4. Explain the current status of N2 fixation in the field of biotechnology. [16]

5. Write short notes on the following

(a) Turbine
(b) Chemostat
(c) Turbidostat
(d) Rheology [4+4+4+4]

6. What are haploids?Explain how anther and pollen cultures are useful for the pro-
duction of Haploids? [16]

7. Explain the process of gene transfer of herbicide tolerance. [16]

8. (a) Explain in detail about the Transfermation by ultrasonication and calcium

phosphate precipitation method.
(b) Shuttle Vectors
(c) Binary Vectors. [8+4+4]

?????

1
Code No: 45056 R07 Set No - 2
III B.Tech I Semester Regular Examinations,Nov/Dec 2009
PLANT BIOTECHNOLOGY
Bio-Technology
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
?????

1. How Ti plasmid transfers T-DNA in host cells? [16]

2. Explain the following

(a) how to produce Basta resistance plants

(b) discuss the mode of action of Glyphosate [8+8]

3. Discuss phenyl alanine - shikonic acid pathway in detail. [16]

4. Give an account on legume symbiosis. [16]

5. Explain how Vitamin A can be expressed in Rice plants. [16]

6. Write about Somoclonal variation in detail Discuss the merits and demerits of
Somoclonal variation. [16]

7. Discuss the techniques of selecting cell lines for high yields of secondary compounds.
[16]

8. What are various methods used to measure growth of cells in cell suspension
cultures? [16]

?????

2
Code No: 45056 R07 Set No - 3
III B.Tech I Semester Regular Examinations,Nov/Dec 2009
PLANT BIOTECHNOLOGY
Bio-Technology
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
?????

1. Explain

(a) Explain the role of reactive oxygen species in stress tolerance

(b) Write short notes on SOD enzyme family in stress tolerance [8+8]

2. Write short note on

(a) Antitumor agents

(b) Embryo rescue [8+8]

3. Give an account on different secondary metabolites of plant origin. [16]

4. Explain the various strategies for insect resistance by Bacillus thuringienesis. [16]

5. Write short notes on molecular farming of proteins? What are different products
produced by this method and the economic area. [16]

6. Explain the role of antisense RNA in enhancing secondary metabolite production.

[16]

7. Write notes on

(a) Callus culture

(b) Soma clonal variations [8+8]

8. Enumerate how the Agrobacterium mediated gene transfers are different from other
viral vectors mediated gene transfer. [16]

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3
Code No: 45056 R07 Set No - 4
III B.Tech I Semester Regular Examinations,Nov/Dec 2009
PLANT BIOTECHNOLOGY
Bio-Technology
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
?????

1. Write short note on any two

(a) Co-cultivation of Agrobacterium

(b) Scorable gene [8+8]

2. Explain the different abiotic stresses involved in creating a water deficit. [16]

3. Discuss the mode of action of the following

(a) Glyphosate
(b) Phosphinothricin
(c) PPT
(d) Atrazine [4x4]

4. Explain with neat diagram how molecule is synthesized by transgenic plants. [16]

5. Discuss the factors affecting pollen cultures. [16]

6. Write short notes on

(a) Continuous culture

(b) Bergman’s cell plating technique
(c) Idiolites. [5+5+6]

7. (a) What is Organogenesis?

(b) Explain the process of initiation and maintainance of Callus Cultures. [4+12]

8. “Plant tissue culture can act as alternative for the production of secondary metabo-
lites” justify the statement with suitable examples. [16]

?????