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A group of students carried out an experiment to study the effect of the concentration of

albumen suspension on the rate of reaction of pepsin enzyme.


Sekumpulan pelajar telah menjalankan satu eksperimen untuk mengkaji kesan kepekatan
ampaian albumen ke atas kadar tindak balas enzim pepsin.
Diagram a shows the method used by the students.
The time taken for the change in the cloudiness of the albumen suspension is shown in
Diagram b.
Rajah a menunjukkan kaedah yang telah digunakan oleh kumpulan pelajar itu.
Masa yang diambil untuk perubahan kekeruhan pada ampaian albumen adalah ditunjukkan
dalam Rajah b.
The whole experiment in Diagram a was repeated using different concentrations of albumen
suspension.
Keseluruhan eksperimen dalam Rajah a itu diulangi dengan menggunakan kepekatan
ampaian albumen yang berbeza.
Table a shows the results of the experiment.
Jadual a menunjukkan keputusan eksperimen tersebut.

Diagram a
Rajah a

Diagram b
Rajah b

Table a
Jadual a
(a (i)Based on Table a, state two observations on the relationship between the quantity of
)
albumen and time.
Berdasarkan Jadual a, nyatakan dua pemerhatian tentang hubungan antara kuantiti
albumen dengan masa.
1 _____________________________________________________________________
. _____________
2
. _____________________________________________________________________
_____________
[3 marks]
[3 markah]
(ii State the inference which corresponds to the observation in (a)(i).
) Nyatakan inferens yang sepadan dengan pemerhatian di (a)(i).
1 _____________________________________________________________________
. _____________
2 _____________________________________________________________________
. _____________
[3 marks]
[3 markah]
(b Using the information provided in Table a, complete Table b by recording the time taken
) for the albumen suspension to turn clear.
Menggunakan maklumat dalam Jadual a, lengkapkan Jadual b dengan merekod masa
yang diambil untuk ampaian albumen menjadi jernih.

Table b
Jadual b
[3 marks]
[3 markah]
(c (i) Complete Table c based on this experiment.
)
Lengkapkan Jadual c berdasarkan eksperimen ini.

Table c
Jadual c
[3 marks]
[3 markah]
(ii) The following list is part of the apparatus and material used in this experiment.
Senarai berikut adalah sebahagian daripada radas dan bahan yang digunakan dalam
eksperimen ini.

Complete Table d by matching each variable with the apparatus and material used in
this experiment.
Lengkapkan Jadual d dengan memadankan setiap pembolehubah dengan radas dan
bahan yang digunakan dalam eksperimen ini.

Table d
Jadual d
[3 marks]
[3 markah]
(d State the hypothesis for this experiment.
) Nyatakan hipotesis bagi eksperimen ini.

_________________________________________________________________________
____________
[3 marks]
[3 markah]
(e (i) Based on Table a, construct a table and record the results of the experiment which
)
includes the following aspects:
Berdasarkan Jadual a, bina satu jadual dan rekodkan keputusan eksperimen yang
meliputi aspek-aspek berikut:
Percentage concentration of albumen suspension
Peratusan kepekatan ampaian albumen
Time/min
Masa/min
Rate of enzyme reaction as percentage of albumen converted per minute
Kadar tindak balas enzim sebagai peratusan perubahan albumen per minit

[3 marks]
[3 markah]
(ii)On the graph paper provided on page below, draw the graph of rate of reaction of
pepsin against the concentration of albumen suspension.
Pada kertas graf yang disediakan di halaman berikut, lukis graf kadar tindak balas
pepsin melawan kepekatan ampaian albumen.
[3 marks]
[3 markah]
(iii Explain the relationship between the rate of reaction of pepsin and the concentration of
) albumen suspension based on the graph in (e)(ii).
Terangkan hubungan antara kadar tindak balas pepsin dan kepekatan ampaian
albumen berdasarkan graf di (e)(ii).
______________________________________________________________________
____________
______________________________________________________________________
____________
[3 marks]
[3 markah]
(f Based on this experiment, what can you deduce about this enzyme?
) Berdasarkan eksperimen ini, apakah yang dapat anda rumuskan tentang enzim ini?
_________________________________________________________________________
____________
_________________________________________________________________________
____________
[3 marks]

[3 markah]
(g The experiment is repeated using the apparatus set up as in Diagram c.
) The quantities of albumen suspension and pepsin enzyme used are as shown.
The experiment is left for one hour.
Eksperimen ini diulang dengan menggunakan set radas seperti dalam Rajah c.
Kuantiti ampaian albumen dan enzim pepsin yang digunakan adalah seperti yang
ditunjukkan.
Eksperimen dibiarkan selama satu jam.

Diagram c
Rajah c
P, Q, R and S are four possible observations after one hour.
Choose one correct observation and explain your choice.
P, Q, R dan S adalah empat kemungkinan pemerhatian selepas satu jam.
Pilih satu kemungkinan pemerhatian yang betul dan terangkan pilihan anda.

_________________________________________________________________________
____________
_________________________________________________________________________
____________
[3 marks]
[3 markah]
Graph of rate of reaction of pepsin against the concentration of albumen suspension
Graf kadar tindak balas pepsin melawan kepekatan ampaian albumen

2 Pleurococcus sp. is a unicellular green alga found on the bark of trees. The population
. distribution of Pleurococus sp. is affected by abiotic factors such as light intensity.
A group of students carried out an experiment to investigate the effect of light intensity on
the population distribution of Pleurococcus sp.
The diagram below shows a tree trunk on which Pleurococcus sp. was growing.
Pleurococcus sp. ialah alga hijau unisel yang terdapat pada kulit batang pokok. Taburan
populasi Pleurococcus sp. dipengaruhi oleh faktor-faktor abiosis, contohnya keamatan
cahaya.
Sekumpulan murid menjalankan satu eksperimen untuk mengkaji kesan keamatan cahaya
ke atas taburan populasi Pleurococcus sp.
Rajah di bawah menunjukkan sebatang pokok yang ditumbuhi Pleurococcus sp.

Diagram
Rajah
Two samples of the distribution of Pleurococcus sp., Grid X and Grid Y, were taken. Grid X
was placed on the tree trunk facing east which received more sunlight. Grid Y was placed
on the tree trunk facing south which received less sunlight.
Dua sampel taburan Pleurococcus sp., Grid X dan Grid Y, telah diambil. Grid X telah
diletakkan pada bahagian batang pokok yang menghadap arah timur yang menerima lebih
cahaya matahari. Grid Y telah diletakkan pada bahagian batang pokok yang menghadap
arah selatan yang menerima kurang cahaya matahari.

Table (a) and Table (b) show the total surface area covered by Pleurococcus sp. on Grid X
and Grid Y.
Jadual (a) dan Jadual (b) menunjukkan jumlah luas permukaan yang dilitupi oleh
Pleurococcus sp. pada Grid X dan Grid Y.

Table (a)
Jadual (a)

Table (b)
Jadual (b)
(a Record the total surface area covered by Pleurococcus sp. in the spaces provided in Table
) (a) and Table (b).
[3 marks]
Catatkan jumlah luas permukaan yang dilitupi oleh Pleurococcus sp. pada ruang yang
disediakan dalam Jadual (a) dan Jadual (b).
[3 markah]
(b (i) State two different observations made from the diagrams in Table (a) and Table (b).
)
Nyatakan dua pemerhatian yang berbeza yang dibuat daripada rajah di Jadual (a)
dan Jadual (b).
Observation 1:
Pemerhatian 1:
_____________________________________________________________________
_____________

_____________________________________________________________________
_____________
Observation 2:
Pemerhatian 2:
_____________________________________________________________________
_____________
_____________________________________________________________________
_____________
[3 marks]
[3 markah]
(ii State the inferences from the observations in (b)(i).
) Nyatakan inferens daripada pemerhatian di (b)(i).
Inference from observation 1:
Inferens daripada pemerhatian 1:
_____________________________________________________________________
_____________
_____________________________________________________________________
_____________
_____________________________________________________________________
_____________
Inference from observation 2:
Inferens daripada pemerhatian 2:
_____________________________________________________________________
_____________
_____________________________________________________________________
_____________
_____________________________________________________________________
_____________
[3 marks]
[3 markah]
(c Complete the table belowbased on this experiment.
) Lengkapkan jadual di bawah berdasarkan eksperimen ini.

Table
Jadual
[3 marks]
[3 markah]
(d State the hypothesis for this experiment.
) Nyatakan hipotesis bagi eksperimen ini.
_______________________________________________________________________
______________
_______________________________________________________________________
______________
_______________________________________________________________________
______________
[3 marks]
[3 markah]
(e (i)Construct a table and record all the data collected in this experiment.
)
Bina satu jadual dan rekodkan semua data yang dikumpul dalam eksperimen ini.
Your table should have the following aspects:
Jadual anda hendaklah mengandungi aspek-aspek berikut:
Title with correct unit

Tajuk dengan unit yang betul


Position of the grid
Kedudukan grid
Total surface area covered by Pleurococcus sp.
Jumlah luas permukaan yang dilitupi oleh Pleurococcus sp.

[3 marks]
[3 markah]
(ii Use the graph paper provided below to answer this question.
) The population of Pleurococcus sp. is represented by the total surface area covered in
the grid.
Using the data in (e)(i), draw a bar chart to show the relationship between the
population of Pleurococcus sp. and the position of the grids.
Guna kertas graf yang disediakan di halaman berikut untuk menjawab soalan ini.
Populasi Pleurococcus sp. diwakili oleh jumlah luas permukaan yang dilitupi dalam
grid.
Menggunakan data di (e)(i), lukis satu carta bar untuk menunjukkan hubungan antara
populasi Pleurococcus sp. dengan kedudukan grid-grid itu.
[3 marks]
[3 markah]
Population of Pleurococcus sp. against the position on the tree trunk
Populasi Pleurococcus sp. melawan kedudukan pada batang pokok

(f Based on the bar chart in (e)(ii), explain the relationship between the population
) distribution of Pleurococcus sp. and the light intensity.
Berdasarkan carta bar di (e)(ii), terangkan hubungan antara taburan populasi
Pleurococcus sp. dengan keamatan cahaya.
_______________________________________________________________________
______________
_______________________________________________________________________
______________
[3 marks]
[3 markah]
(g State the operational definition for population distribution of Pleurococcus sp.
) Nyatakan definisi secara operasi bagi taburan populasi Pleurococcus sp.
_______________________________________________________________________
______________
_______________________________________________________________________
______________
[3 marks]
[3 markah]
(h Lightning strikes the tree and causes the tree to fall. The Pleurococcus sp. under study is
) then exposed to direct sunlight from 7.00 a.m. till 6.00 p.m. daily.
Based on the results of this experiment, predict what will happen to the total surface area
covered by the Pleurococcus sp. after one week.
Explain your prediction.
Kilat menyambar pokok tersebut dan mengakibatkan pokok itu tumbang. Pleurococcus
sp. yang dikaji terdedah secara terus kepada cahaya matahari dari jam 7.00 a.m. hingga
6.00 p.m. setiap hari.
Berdasarkan keputusan eksperimen ini, ramalkan apa yang akan berlaku kepada jumlah
luas permukaan yang dilitupi oleh Pleurococcus sp. selepas satu minggu.
Terangkan ramalan anda.
_______________________________________________________________________
______________
_______________________________________________________________________
______________
[3 marks]
[3 markah]
(i)The following is a list of biotic and abiotic factors.
Berikut ialah senarai faktor-faktor biosis dan abiosis.

Classify these factors in the table below.

Klasifi kasikan faktor-faktor ini dalam jadual di bawah.

Table
Jadual
[3 marks]
[3 markah]

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