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12/08/13 20:45
We are taught that plasma membranes are a typical lipid bilayer, but how do we know this, and who figured it
out?
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Most books mention that membranes have a typical "lipid bilayer," but why lipids, why should it be a bilayer, and how was this basic structure determined? Although it is now
generally taken for granted that membranes are based on the presence of a lipid bilayer, that was not always the case. Early experiments, often by physicists, led to the
understanding that the cell membrane was lipid in nature. A key experiment using the Langmuir trough provided the basis for accepting that the membrane is a bilayer and laid
the groundwork for the current model of membrane structure.
concentration. Most cells are surrounded by a dilute aqueous medium, which means that key compounds
would, if not prevented, constantly leave the cell. The answer to this problem is to enclose the cytoplasm with a membrane that prevents the free movement of molecules. As
described above, a nonpolar material would work well for this membrane because it would not wash away in the surrounding water and would not dissolve water-soluble
substances out of the cell.
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measuring the original volume of oil and the final area it covered, he was able to calculate the thickness of
the film. This initial observation was improved on by the work of Agnes Pockels. Working in her kitchen, and
with no formal training, she devised a simple apparatus to quantify the area covered by the oil film. Her
apparatus was refined by Langmuir (1917) and is now generally referred to as a Langmuir trough (Figure 2),
although it really should be a Pockels trough.
In 1925, Gortner and Grendel performed some key experiments using a Langmuir trough and blood cells
(Gortner & Grendel 1925). They were interested in determining the amount of lipid in the membranes of red
blood cells. Why use red blood cells? These cells were an excellent choice for this experiment because they
have no nucleus or other membrane-bound organelles in the cytoplasm; therefore, any membrane lipids that
are found must be those that make up the plasma membrane. First, the scientists extracted the lipids with a
variety of solvents, including acetone, from a known number of cells. Then they used the Langmuir trough to
determine how large an area the lipids could cover. Because they could measure the actual size (surface area)
of a red blood cell and knew approximately how many of those cells they had in their sample, they could
calculate the total surface area that would have to be covered by membrane. When the two numbers were
compared, it was clear that the amount of lipid they had extracted could cover
enclose all the cells. Why would there be so much? Additional experiments showed that lipids could
spontaneously form a bilayer when mixed with water (Figure 1). Together, these observations suggested that
there may be a simple explanation for the results with the red blood cells. The plasma membrane of these cells likely consists of a
cell.
As it happens, Gortner and Grendel made some errors in their experiment. They failed to completely extract all the lipids from the cells, and they also underestimated the total
surface area of the individual red blood cells. However, because these two errors canceled each other out, their final conclusions turned out to be correct, regardless of their
miscalculations. Thereafter, the idea of a lipid bilayer became the basis for future models of membrane structure. (Sadava 1993).
Summary
The first clues to lipid bilayer structure came from results with red blood cell membranes. The ultimate discovery that the plasma membrane is a lipid bilayer with hydrophobic
and hydrophilic properties changed the way this structure was viewed. Its semipermeable and liquid nature provided the groundwork for understanding both its physical and
biological properties.
Langmuir, I. The constitution and fundamental properties of solids and liquids II: Liquids.
Overton, E. The probable origin and physiological significance of cellular osmotic properties.
(1899). In
Sadava, D. E.
Cell Biology, Organelle Structure and Function. Boston: Jones and Bartlett, 1993.
Ben Franklin Stilled the Waves. Durham, NC: Duke University Press, 1989.
Cell Membranes
INTERMEDIATE
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Mitochondria
Endosomes in Plants
ADVANCED
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