Product Specification Sheet

Product Name

Stemolecule ROCK II Inhibitor

Description

Stemolecule ROCK II Inhibitor is a specific inhibitor for type 2 Rho-associated

1
protein kinase (ROCK) . ROCKs are serine/threonine-specific protein kinases
2
which play a crucial role in organization of the actin cytoskeleton . ROCK
inhibitors have been found to prevent apoptosis, as well as enhance the survival
and cloning efficiency of dissociated human embryonic stem (ES) cells without
2,3
affecting their self-renewal properties or pluripotency .

Catalog Number

04-0030

Size

2 mg

Alternate Name

N-(2-(2-(dimethylamino)ethoxy)-4-(1H-pyrazol-4-yl)phenyl)-2,3
dihydrobenzo[b][1,4]dioxine-2-carboxamide

Chemical Formula

C22H24N4O4

Structure

Molecular Weight

408.45

CAS Number

N/A

Purity

Greater than 98% by HPLC analysis

Formulation

White powder

Solubility

For a 10 mM concentrated stock solution of ROCK II Inhibitor, reconstitute the

compound by adding 489.7 l of DMSO to the entire contents of the vial. If
precipitate is observed, warm the solution to 37C for 2 to 5 minutes. For cell
culture, the media should be prewarmed prior to adding the reconstituted
compound. Note: for most cells, the maximum tolerance to DMSO is less than
0.5%. This molecule is soluble in DMSO at 100 mM.

Storage and Stability

Store powder at 4C protected from light. Following reconstitution, store aliquots

at -20C. Stock solutions are stable for 6 months when stored as directed.

Quality Control

The purity of ROCK II Inhibitor was determined by HPLC analysis. The accurate
mass was determined by mass spectrometry. Cellular toxicity of ROCK II Inhibitor
was tested on mouse embryonic stem cells.

For research use only. Not for use in diagnostic procedures.

Stemgent 2011. Unless otherwise noted, Stemgent, Stemgent Logo and all other trademarks
are the property of Stemgent, Inc.
Stemgent, 10575 Roselle St., San Diego, CA 92121 www.stemgent.com v2.0

Product Specification Sheet

References

1.

2.
3.

Feng, Y., Yin, Y., Weiser, A., Griffin, E., Cameron, M.D., Lin, L., Ruiz, C.,
Schurer, S.C., Inoue, T., Rao, P.V., Schroter, T., and Lograsso, P. (2008)
Discovery
of
substituted
4-(pyrazol-4-yl)-phenylbenzodioxane-2carboxamides as potent and highly selective Rho kinase (ROCK-II) inhibitors.
J Med Chem 51: 6642-6645.
Olson, M.F. (2008) Applications for ROCK kinase inhibition. Curr Opin Cell
Biol 20: 242-248.
Watanabe, K., Ueno, M., Kamiya, D., Nishiyama, A., Matsumura, M., Wataya,
T., Takahashi, J.B., Nishikawa, S., Muguruma, K., and Sasai, Y. (2007) A ROCK
inhibitor permits survival of dissociated human embryonic stem cells. Nat
Biotechnol 25: 681-686.

For research use only. Not for use in diagnostic procedures.

Stemgent 2011. Unless otherwise noted, Stemgent, Stemgent Logo and all other trademarks
are the property of Stemgent, Inc.
Stemgent, 10575 Roselle St., San Diego, CA 92121 www.stemgent.com v2.0