SPE phases for food analysis

Diamino

special silica phase for determination

of pesticides in food samples

Base material silica, pore size 60 , particle size

45 m, specific surface 500 m2/g, pH stability 28
Primary and Secondary Amine functions (PSA), 5 % C
Removes polar compounds (e.g., organic acids, pigments, sugars) from matrices like fruit or vegetables
Similar phases: Supelclean PSA, Bond Elut PSA

Solid Phase Extraction

QuEChERS method and pre-mixes

Within a few years after its development by Anastassiades
et al. the QuEChERS method has gained a leading position for determination of pesticide residues in food
samples by GC-MS or LC-MS, allowing rapid and cheap
clean-up of strongly matrix-contaminated samples.

special SPE phase for quick and cheap

determination of pesticides in strongly
matrix-contaminated samples by GC or
HPLC (QuEChERS method = Quick Easy
Cheap Effective Rugged Safe)

In addition to the required adsorbent CHROMABOND

Diamino MACHEREY-NAGEL offers a number of individually weighed and premixed extraction and buffer
mixtures, specially composed for different sample matrices.

Standard clean-up of food samples

For extraction, the European standard EN 15662 recommends a citrate extraction mix (Mix I), while AOAC
standard 2007.1 uses an acetate extraction mix (Mix II).

10 g sample are homogenized with 10 mL acetonitrile. After adding the internal standard the sample is shaken with 4 g MgSO4
and 1 g NaCl and afterwards centrifuged.
1 mL of the supernatant is spiked with 25 mg CHROMABOND
Diamino and 150 mg MgSO4 and shaken again. After centrifugation the supernatant is injected into GC/MS.

For clean-up, the Diamino phase (PSA) removes, e.g.,

sugars and organic acids. MgSO4 removes water, C18 ec
removes nonpolar interferences such as fats and the
Carbon phase removes pigments, sterols, and nonpolar
interferences.

MN Appl. No. 303770

For selection of the proper clean-up mix see table on

opposite page.

For optimizing the extraction of pH-dependent compounds, for minimizing decomposition of sensitive
substances, and for broadening the matrix spectrum,
different modifications of the QuEChERS method have
been elaborated.

48

Recommended application:

For detailed instructions please visit

www.mn-net.com or the original references at
www.quechers.com.

info@mn-net.com

SPE phases for food analysis

Ordering information
Volume Description
12
10
8

Composition

CHROMABOND

Pack of

730970

50

730971

50

730972

50

730973

50

730975

50

730974

50

730561
730562

50
30

730653.20
730653

20 g
100 g

730223

50

QuEChERS extraction buffer mixes

15 mL* Mix I

citrate extraction mix

15 mL* Mix II

acetate extraction mix

6
4

REF

4 g MgSO4, 1 g NaCl, 0.5 g Na2H citrate 1.5 H2O, 1 g Na3 citrate 2 H2O
6 g MgSO4, 1.5 g Na acetate

CHROMABOND QuEChERS clean-up mixes

15 mL* Mix III

Diamino clean-up mix

15 mL* Mix IV

Diamino/Carbon clean-up mix

15 mL* Mix V

Diamino/Carbon clean-up mix

15 mL* Mix VI

Diamino/C18 ec clean-up mix

0.15 g CHROMABOND Diamino

with 0.9 g MgSO4
0.15 g CHROMABOND Diamino
with 0.9 g MgSO4 and 15 mg Carbon
0.15 g CHROMABOND Diamino
with 0.9 g MgSO4 and 45 mg Carbon
0.15 g CHROMABOND Diamino
with 0.9 g MgSO4 and 150 mg C18 ec

3 mL adsorbent weight 200 mg

6 mL adsorbent weight 500 mg

Solid Phase Extraction

CHROMABOND Diamino polypropylene columns

CHROMABOND Diamino adsorbent

CHROMABOND QuEChERS accessories

50 mL polypropylene centrifuge tube with screw cap
* 15 mL centrifuge tubes with screw cap (2 mL or 50 mL centrifuge tubes on request)
A number of custom-made QuEChERS mixes is available on request.

QuEChERS mixes
Sample property
Low fat content
Moderate content of chlo(e.g., apples, strawberries) rophyll and carotinoids
(e.g., carrots, lettuce)

Higher content of chloro- Higher fat content

phyll and carotinoids (e.g., (e.g., avocado)
bell peppers, spinach)

CHROMABOND QuEChERS extraction mixes

Citrate or acetate extraction
Mix I or Mix II

Citrate or acetate extraction

Mix I or Mix II

Citrate extraction
Mix I

Citrate extraction
Mix I

CHROMABOND QuEChERS clean-up mixes

Diamino clean-up
Mix III

www.mn-net.com

Diamino/Carbon clean-up
Mix IV

Diamino/Carbon clean-up
(higher Carbon content)
Mix V

Diamino/C18 ec clean-up
Mix VI

49

Summary of MN phases for SPE

Code

Matrix

Modification / Application

Similar phases*

SA

silica

benzenesulfonic acid cation exchanger

(SCX)

PCA

silica

propylcarboxylic acid cation exchanger

(WCX)

Strata SCX Bond Elut SCX DSC-SCX, LC-SCX

CLEAN-UP Benzenesulfonic Acid Accubond
SCX Bakerbond Aromatic Sulfonic Acid Isolute SCX LiChrolut SCX
Strata WCX Bond Elut CBA DSC-WCX, LCWCX CLEAN-UP Carboxylic Acid Bakerbond
Carboxylic Acid Isolute CBA

PSA
HR-XC

silica
PS/DVB

HR-XA

PS/DVB

HR-XCW

PS/DVB

HR-XAW

PS/DVB

PS-OH
PS-H+
PS-Mix
PS-Ag+
PS-Ba2+

PS/DVB
PS/DVB
PS/DVB
PS/DVB
PS/DVB

propylsulfonic acid cation exchanger

strong mixed mode cation exchanger
for basic analytes (MCX)
strong mixed mode anion exchanger
for acidic analytes (MAX)
weak mixed mode cation exchanger
for basic analytes (WCX)
weak mixed mode anion exchanger
for acidic analytes (WAX)
strong anion exchanger in OH form
strong cation exchanger in H+ form
mixture of PS-OH and PS-H+
strong cation exchanger in Ag+ form
strong cation exchanger in Ba2+ form

Page
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35

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16

Oasis MCX Strata-X-C

HyperSep Retain-CX Styre Screen DBX
Oasis MAX Strata-X-A
HyperSep Retain-AX Styre Screen QAX
Oasis WCX Strata X-CW

17

Oasis WAX Strata X-AW

19

18

Solid Phase Extraction

22
22
22
22
22

Phases for special applications

Dry
Drug

Na2SO4
silica

for drying organic samples

bifunctional C8/SA, for enrichment of
drugs from urine

Strata Screen-C Bond Elut Certify I DSCMCAX Clean Screen DAU Accubond Evidex
Bakerbond Narc-2 Isolute HCX LiChrolut
TSC HyperSep Verify CX
Strata Screen-A Bond Elut Certify II Clean
Screen THC Bakerbond Narc-1 Isolute
HAX HyperSep Verify AX

bifunctional C8/SB, for extraction of THC

and derivatives and of acidic analytes
from biological fluids
Crosslinks
cellulose for enrichment of collagen crosslinks
Tetracycline silica
special octadecyl phase, for enrichment
of tetracyclines
HR-P-AOX
PS/DVB
for extraction of AOX from water (DIN
38409 H22)
CN/SiOH
silica
combination phase for enrichment of
PAHs from soil
silica
combination phase for enrichment of
NH2/C18
PAHs from water
combination phase for extraction of hyNa2SO4/Florisil
drocarbons from water (DIN H-53 / ISO
DIS 9377-4)
SA/SiOH
silica
combination phase for enrichment of PCB Bakerbond PCB-N
from waste oil
combination phase, used together with
SiOH-H2SO4/ silica
SA
SiOH for enrichment of PCB from oil
combination phase for enrichment of PCB
NAN
silica /
AgNO3 + from sludge
Na2SO4
ABC18
silica
octadecyl, with ion exchange functions,
Isolute M-M
for acrylamide analysis
Diamino
silica
primary and secondary amine functions
Supelclean PSA, Bond Elut PSA
(PSA), for determination of pesticides in
food samples (QuEChERS method)
Phase separation
CHROMABOND PTL/PTS
EXtrelut Chem Elut Hydromatrix
Liquid-liquid extraction CHROMABOND XTR
* Phases which provide a similar selectivity based on chemical or physical properties (list not complete)
Drug II

silica

www.mn-net.com

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