Professional Documents
Culture Documents
SYLLABUS
(For the Academic year 2015 2016)
I IV Semester M. Tech
Master of Technology
(Biotechnology)
ORGANIZATION CHART
Dr. NVR Naidu
Principal
Dr. T.V. Suresh Kumar
Registrar (Academics)
Faculty Name
Qualification
Designation
Specialization
Dr. Channarayappa
MSc (Agri),
Ph.D.(India), Ph.D.
(USA), PDF (USA),
PGDBA
Professor &
Head of the
Department
Biotechnology,
Molecular biology
interdisciplinary approach,
Agriculture
Dr. Bindu S
Associate
Professor
Food Biotechnology
Toxicology
Dr. Chandraprabha MN
Associate
Professor
Biochemical Engg.
Environmental BT
Dr. Dhamodhar P
Associate
Professor
Immunotechnology
Biochemistry
Mr. Lokesh KN
M. Pharm (Ph.D.)
Assistant
Professor
Pharmaceutical Biotechnology
Dr. Ahalya N
Associate
Professor
Microbiology
Environmental Biotechnology
Dr. Sharath R
MSc, Ph.D.,
Assistant
Professor
Assistant
Professor
Cancer Biology
Virology
Dr. Prabha M
Assistant
Professor
Dr. Sravanti V
M.Sc., Ph.D.
Assistant
Professor
Mrs. Samrat K
M. Tech
Assistant
Professor
Nano-Biotechnology,
Microbial Biotechnology
Mr. Gokulakrishna M
M. Tech
Assistant
Professor
Ms. Bhavya SG
M. Tech
Assistant
Professor
Enzyme Technology
Downstream processing
M. Tech
Assistant
Professor
Bioinformatics, Biochemical
and Bioprocess Engineering
Dr. Pradeepa K
M.Sc., Ph.D.
Assistant
Professor
Plant biotechnology
Phytochemistry-Pharmaco
To Evolve into an autonomous institution of international standing for imparting quality technical
education
MSRIT shall deliver global quality technical education by nurturing a conducive learning
environment for a better tomorrow through continuous improvement and customization
PROGRAM OUTCOMES
By the time of Post graduation in Biotechnology Engineering, students should be able to:
a. Imbibe the essential concepts of both engineering and life sciences &apply it to a wide range of
interdisciplinary work.
b. Understand the engineering design, conduct experiments in biotechnology and apply in the
field by generating innovative, economical and feasible solutions.
c. Design and automate the processes and programs to accelerate the output for wide applications
d. Perform and formulate both concept and empirical based equations and formulas in
biotechnology to solve the problems and to draw meaningful conclusions.
e. Update the modern techniques, skills and advanced engineering tools essential for applications
in biotechnology.
f. Mould the students behavior, attitude and interpersonal skills to function in multi-disciplinary
teams and setups.
g. Become a responsible citizen by being aware of his/her roles, duties, professional and ethical
responsibilities and rights.
h. Develop soft-skills through classroom seminars, institutional and industry interactions, use of
modern research and teaching aids
i. Encourage students to take courses from other branches of engineering to have broad-based
education and multidisciplinary approach in a global and societal context
j. Create enthusiasm in the candidate for life-long learning and urge to contribute to technology
and society by working in a need-based and problem solving projects.
k. Possess knowledge of contemporary issues for sharpening managerial and entrepreneurial
skills to commercialize the technology & capture the markets for innovations.
a b c d e f
H H H H
g h i
M
L H H M
j k
L
M H H M
L L
L H H H
L L
L M H M L L
L M
H M
L M H
LH L
L L
L L L H H
L L M H H
L L
M L H H
L H
L M H M
H H
Improvements base d on periodic re view meeting outcomes and deliberations during BOS/BOE will
close the system loop and the process will continue towards quality improvement
Good
Governance
FOCUSED
EDUCATION
Learning
outcomes
10
CREDIT DISTRIBUTION
Total number of credits required to Graduate: 100
Core courses
Electives
Seminars
30%
Research work
37%
7%
26%
Semester
I
II
III
IV
Total
Semester
I
II
III
IV
Total
Core
Courses
17
13
04
34
Course structure:
Breakdown of credits for the M. Tech Degree Curriculum
Electives
Seminars
Lab
Research
Total
work
04
01
4
26
08
01
4
26
12
02
8
26
02
20
22
24
06
8
28
100
Credit distribution Based on L:T:P:S
Credits*
L
T
P
15
6
4
15
6
4
13
5
8
0
2
20
43
21
36
11
S
1
1
0
0
2
Total
26
26
26
22
100
Different stakeholders and their interactions contributed for establishment of outcome based
education.
12
Name
Dr. Channarayappa
Dr. Shivaprakash MK
Dr. BS Gowrishankar
Dr. Nagendra HG
Dr. Bindu S
Dr. P Dhamodhar
10
Dr. Sharath R
13
Chairman /
Member
Chairman
Member
Member
Member
(VTU
Nominee)
Member
(Industry)
Member
(Industry)
Member
Alumnus
Member
Member
Member
M-Tech in Biotechnology
SCHEME OF TEACHING FOR THE ACADEMIC YEAR 2013-2014
Sl. No.
1
2
3
Subject
Code
MBT 101
MBT 102
MBT 103
4
5
6
7
8
MBT 104
MBTE
MBT 106
MBT 107L
MBT 108L
Sl. No.
1
2
3
4
5
6
7
8
Sl. No.
1
2
3
4
5
6
Subject
Code
MBT 201
MBT 202
MBT 203
MBTE
MBTE
MBT 206
MBT 207L
MBT 208L
Subject
Code
MBT 301
MBTE
MBTE
MBTE
MBT 305
MBT 306
Credits*
T
P
S
1
0
1
1
0
0
1
0
0
1
0
1
0
0
5
0
0
0
2
2
4
0
0
0
0
0
1
Total
5
4
4
4
4
1
2
2
26
T
1
1
1
0
0
1
0
0
4
Credits*
P
S
0
1
0
0
0
0
0
0
0
0
0
0
2
0
2
0
4
1
Total
5
4
4
4
4
1
2
2
26
T
1
0
0
0
0
2
3
Credits*
P
S
0
0
0
0
0
0
0
0
8
0
0
0
8
0
Total
4
4
4
4
8
2
26
Subject
Code
MBT 405
MBT 406
14
Sl.
No.
1
2
3
4
5
6
7
8
9
10
11
12
13
*L, Lecture;
T, Tutorial;
15
Total
4
4
4
4
4
4
4
4
4
4
4
4
4
: MBT 101
: 3:1:0:1
CIE
SEE
: 50 Marks
: 50 Marks
UNIT 1
Cell biology: Cell structure: prokaryotic and eukaryotic cells, Cytoskeleton, Structure and functions of
nucleus, mitochondria, ribosome, Golgi bodies, lysosomes, endoplasmic reticulum, peroxisomes,
chloroplasts, vacuoles, plasma membrane. Fluid mosaic theory of plasma membrane. Active and
passive transport systems of plasma membrane. Cell division and cell cycle regulation (mitosis and
meiosis). Cellular and sensory communications.
UNIT 2
Genetics and inheritance: Introduction to Mendelian genetics, Gene interactions and exceptions to
Mendelian laws. Different types of gene interactions. Structure and organization of genetic material in
eukaryotes, structure of chromosomes, special chromosomes. Linkage and recombination.
Introduction to microbial genetics.
UNIT 3
Plants: Photosynthesis: light and dark reaction, CO2 fixation, respiration and photorespiration,
electron transport chain and ATP synthesis in mitochondria.
Animals: Introduction to human digestive, circulation, respiration, excretion and reproductive
systems. Nervous systems: peripheral and CNS. Introduction to immune system: innate and adaptive
immunity, antibody structure and functions,
UNIT 4
Introduction to metabolic engineering: review of cellular metabolism, models for cellular reactions,
material balance and data consistency- Block box model, elemental balance, heat balance and analysis.
Regulation of enzyme activities and concentration. Regulation of transcription and translation.
Regulation of metabolic networks.
UNIT 5
Manipulation of metabolic pathways: enhancement of product yield and productivity-ethanol, amino
acids and solvents. Product spectrum and novel products: antibiotics, polyketides, vitamins and
biological pigments. Metabolic flux analysis: methods for determination of metabolic fluxes by
isotope labeling-fractional label enrichment. Application of metabolic flux analysis; amino acid
production by Glutamic acid bacteria and mammalian cell cultures, flux analysis of metabolic
networks- bottom-up and top-down approach.
Textbooks:
1. Channarayappa (2010) Cell biology, Universities Press (India Pvt Ltd., Hyderabad.
16
2. Hardin J et al. (2012)Beckers World of the Cell VIII edn. Pearson Benjamin Cummings, San
Francisco, USA
3. Gregory N. Stephanopounlos, Aritstos A. Aristidou and Jens Nielsen (1998) Metabolic
Engineering: principles and methodologies. Academic Press, USA.
Reference Books
1. Shuler Ml and Kargi F (2010) Bioprocess engineering basic concepts, 2nd Edn. Prentice Hall.
2. Cortassa S, Aon MA, Lglesias AA and Lyod L (2002) An introduction and metabolic and
cellular Engineering. World Scientific Publications Pvt Ltd. Singapore.
3. Sujit K Chaudhuri (2010) Concise Medical Physiology, New Central Book Agency, Pvt. Ltd.
4. Pierce BA. (2012), Genetics A Conceptual Approach IV edn. W. H. Freeman and Company
New York
5. Bruce A. et al (2007) Molecular Biology of the Cell, 5th edition, Garland science, New
York,USA.
6. Nestor V. Torres and Eberhard O. Voit (2002) Pathways and optimization in metabolic
Cambridge University Press.
Course Delivery: Regular black Board teaching and interaction through tutorial class
Indirect
Assessment
Methods
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
10
Assignment
reports
1,2,3&4
10
Quiz
answers
1,2,3&4
100
Answer
scripts
1,2,3&4
Middle of the
course
Feedback
forms
End of course
Questionnaire
CIE
SEE
Standard
examination
Students feedback
Students
End of course
survey
1,2,3&4,
delivery of the
course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various educational
components
S.No
1
2
3
4
Blooms Category
Remember
Understand
Apply
Analyze
Test 1
25
35
25
15
Test 2
20
30
30
15
17
Test 3
20
30
30
10
Semester-End Examination
25
30
30
10
5
6
Evaluate
Create
0
0
5
0
10
0
5
0
Course Outcome: On completion of this course student will have improved ability:1. To describe basic biological concepts and principles.
2. To appreciate the different levels of biological organization.
3. To understand that biology has a chemical, physical, and mathematical basis and to explain the
importance of the scientific method to understand natural phenomena.
4. To integrate modern biology with engineering principles
Mapping of course outcome with program outcomes
Program Outcome
Course
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8
Outcomes
1.
2.
3.
4.
PO9 PO10 PO
11
PO
12
X
X
X
X
X
X
X
X
: MBT 102
: 3:1:0:0
CIE
SEE
: 50 Marks
: 50 Marks
Advanced techniques used in recombinant DNA technology: Isolation of DNA and RNA.
Estimation of purity and quantity of nucleic acids, Polymerase chain reaction (PCR),
Autoradiography, DNA sequencing. Detection of DNA, RNA and proteins by Southern blotting,
Northern blotting, western blotting and in situ hybridization techniques. Site-specific mutagenesis,
Gene mapping and Microarrays.
UNIT-4
Applications of recombinant DNA technology in Medicine and Health: Production of specialty
chemicals and proteins: secondary metabolites, phytochemicals. Genetically modified microbes
(Recombinant bacteria) for the production of commercial scale production of proteins and
pharmaceuticals, antibiotics, enzymes, insulin, growth hormones, monoclonal antibodies. Applications
rDNA in diagnosis of pathogens and abnormal genes. Transgenic animals. Transgenic animals for
production of proteins and pharmaceuticals. Genetically modified insect cells for the production of
commercially important bioproducts.
UNIT-5
Applications of recombinant DNA technology in agriculture, industry and environment:
Transgenic plants, Transgenic crops for increased yield, resistance to biotic and abiotic stresses,
clearing oil spills. Application of transgenic plants. Industrial production of specialty chemicals and
proteins: organic molecules and commercially important proteins. Biosafety regulations and
evaluation of genetically modified microorganisms (GMOs), plants and animals.
Text books:
1. Channarayappa (2006) Molecular Biotechnology: Principles and Practices. Universities Press
(India) Pvt. Ltd. Worldwide publishing: CRC Press, Taylor and Francis.
2. Russell, David W, Sambrook, Joseph (2001). Molecular cloning: a laboratory manual.
Volumes I - III. Cold Spring Harbor laboratory Press, USA.
Reference Books:
1. David S Latchman (1994) From Genetics to Gene Therapy the molecular pathology of
human disease by, BIOS scientific publishers, sixth edition.
2. Old RW and Primrose SB (1993) Principles of gene manipulation, an introduction to genetic
engineering. Blackwell Scientific Publications.
3. Benjamin Lewis (2008) Genes VIII. Oxford University & Cell Press
4. Channarayappa (2010) Cell Biology. Universities Press (India) Private Limited.
5. Molecular Biology (2015) Universities Press (India) Private Limited.
Course Delivery: Regular black Board teaching and interaction through tutorial class
CIE
Direct Assessment
Methods
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
10
Assignment
reports
1,2,3&4
10
Quiz
answers
1,2,3&4
SEE
Indirect
Assessment
Methods
Standard
examination
End of course
(Answering 5 of
10 questions)
100
Answer
scripts
Middle of the
course
Feedback
forms
End of course
Questionnaire
Students feedback
1,2,3&4
1,2,3&4,
delivery of the
course
1,2,3&4,
assessment
methods
Students
End of course
survey
Questions for CIE and SEE will be designed to evaluate the various educational
components
Blooms Category
Remember
Understand
Apply
Analyze
Evaluate
Create
SL NO.
1
2
3
4
5
6
Semester-End Exam
30
30
20
25
5
0
Course Outcome: On completion of this course student will have improved ability:1. To know the importance of recombinant DNA technology and its applications.
2. To engineer the genes to regulate expression and produce biomolecules.
3. To develop genetically modified organisms (GMOs) of microbes, animals and plants that can
produce industrially important compounds.
4. To assess the advantages and disadvantages of recombinant DNA technology and its future
applications.
Mapping of course outcome with program outcomes
Course Outcomes
1.
2.
3.
4.
a
X
X
X
X
B
X
X
X
X
c
X
X
X
X
D
X
X
X
X
Program Outcome
e
f
g
h
X
X
X
X
X
X
X
X
X
X
i
X
X
X
X
j
X
X
X
X
k
X
X
X
X
: MBT 103
: 3:1:0:0
CIE
SEE
: 50 Marks
: 50 Marks
3. Understand strategies for development of novel Bioprocessing protocol by applying the concise
principles of upstream and downstream processing.
4. Understand principles of different methods of characterization of biomolecules and formulation
strategies in order to enhance shelf life of product.
UNIT 1
Media development and Design of Biological reactors: Introduction, Types of cell culture media,
components of animal origin, inoculum development strategies, Ideal reactors, Reactor dynamics,
Sterilization of reactors, Immobilized biocatalysts, Multiphase Reactors, Animal and plant cell reactor
technology.
UNIT 2
Mammalian and plant cell culture technology: Introduction. Cell line transfection and selection,
Increase in efficiency in selecting a producer cell line, Stability of gene expression, Optimization of
the fermentation process, Bioreactors. Bioreactor consideration for plant cells.
UNIT 3
Cell disruption and protein enrichment operations: Centrifugation; Sedimentation; Flocculation;
Microfiltration; Sonication; Bead mills; Homogenizers; Chemical lysis; Enzymatic lysis. Membrane
based
purification:
Ultrafiltration;
Reverse
osmosis;
Dialysis;
Diafiltration;
Pervaporation;precipitation (Ammonium sulfate solvent). Extraction (solvent aquious two phase,
supercritical)..
UNIT 4
Adsorption and chromatography: size, charge, shape, hydrophobic interactions, Biological affinity;
Process configurations (packed bed, expanded bed, simulated moving beds).Electrophoretic technique,
Electrophoresis.
UNIT 5
Product polishing techniques; Crystallization; Drying. Case studies; product formulation and additives,
freeze drying process.
Text Books:
1. Harris ELV and Angal S (1988) Protein Purification Methods, Ed. IRL Press at Oxford
University Press.
2. Belter PA, Cussler EL and Wei-Shou Hu (2001) Bioseparations-Downstream Processing for
Biotechnology, Wiley-Interscience Publication.
Reference Books:
1. Michael Butler (2007) Cell Culture and Upstream Processing, T & F informa.
2. James E. Bailey and David F. Ollis (1997) Bioprocess Engineering fundamentals. Mc Graw Hill
Book Publication.
3. Bailey JE and Ollis DF (2010) Biochemical Engineering Fundamentals, 2nd Edn, Mc-Graw Hill,
Inc.
4. Scopes RK Berlin (1982) Protein Purification: Principles and Practice, Springer.
Course Delivery: Regular black Board teaching and interaction through tutorial class
Assessment and Evaluation Vis--vis Course outcome
What
To whom When/ Where
(Frequency in
the course)
21
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
Indirect
Assessment
Methods
SEE
CIE
Internal
assessment
tests
Class-room
open book
assignment
Surprise
Test
Students
Standard
examination
Students feedback
Thrice(Average
of the best two
will be
computed)
Twice( Average
of the two will
be computed)
30
Blue books
1,2,3&4
10
Assignment
reports
1,2,3&4
Once
10
Quiz
answers
1,2,3&4
End of course
(Answering 5 of
10 questions)
100
Answer
scripts
1,2,3&4
Middle of the
course
Feedback
forms
End of course
Questionnaire
Students
End of course
survey
1,2,3&4,
delivery of the
course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various educational
components
SL NO.
Blooms Category
Semester-End Exam
1
Remember
30
2
Understand
30
3
Apply
15
4
Analyze
20
5
Evaluate
5
6
Create
0
Course Outcome: On completion of this course student will have improved ability:1. To better understand the applied concepts of upstream and downstream processing.
2. To acquire real-time working knowledge on media formulation, sterilization and optimization
process which are considered as pre-requisites of upstream processing.
3. To execute precise and efficient bioseparation process, which in cost effective and yield high
degree of pure substance.
4. To develop novel Bioprocess which is gives high resolution, economical bioproducts.
Mapping of course outcome with program outcomes
Course Outcomes
1.
2.
3.
4.
a
X
X
X
X
b
X
X
X
X
c
x
X
X
Program Outcome
d
e
f
g
X
X
X
X
X
X
X
X
X
X
X
X
h
X
X
X
X
X
x
22
Sub Code
Credit
: MBT 104
: 3:1:0:0
CIE
SEE
: 50 Marks
: 50 Marks
UNIT-5
Environmental Biotechnology: Treatment of Wastes in Industry- Wastes from Major Industries,
Systems for the Treatment of Wastes- Aerobic breakdown of raw waste waters, Treatment of the
Sludge- Anaerobic Breakdown of Sludge. Hazardous waste management - Bioremediation, Biological
detoxification- examples of biotechnological applications for hazardous waste management. Mining
and Metal biotechnology, microbial transformation, accumulation and concentration of metals, metal
leaching, extraction and future prospects.
Textbooks:
1. Modern industrial microbiology and biotechnology (2007) nduka okafor, science publishers.
2. Industrial Microbiology (2002), Prescott and Dunns, AVI Publishing Company Inc.
3. Environmental Biotechnology (2005), Alan Scragg, oxford university press.
Reference Books:
1. Stanbury PE, Whitaker A, and Hall SJ (1999) Principles of Fermentation Technology by,
Butterworth Heineman, Aditya Books (P) Ltd.
2. Wulf Crueger and Anneliese Crueger, (2002) A text book of Industrial Microbiology, Panima
Publishing Corporation.
Course Delivery: Regular black Board teaching and interaction through tutorial class
Indirect
Assessment
Methods
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
10
Assignment
reports
1,2,3&4
10
Quiz
answers
1,2,3&4
End of course
(Answering 5 of
10 questions)
100
Answer
scripts
1,2,3&4
Middle of the
course
Feedback
forms
End of course
Questionnaire
CIE
SEE
1,2,3&4,
delivery of the
course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various educational
components
SL NO.
Blooms Category
Semester-End Exam
24
1
2
3
4
5
6
Remember
Understand
Apply
Analyze
Evaluate
Create
30
30
15
20
5
0
Course outcomes: Upon completion of this course, the students will be able to:
1. Describe the methods involved in isolation and screening of potential microorganisms for
production of industrial bioproducts.
2. Analyze the different metabolic pathways and its metabolism for the production of desired
processes.
3. Choose and manage appropriate mechanism of microbiological processes to produce
microbiological products.
4. Apply the principles of waste management to treat waste form the bioprocess industries.
Mapping of course outcome with program outcomes
Course Outcomes
1
2
3
4
A
X
X
X
X
b
X
X
X
X
Program Outcome
d
e
f
g
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
PRACTICAL I
Sub Code
Credit
: MBT 107L
: 0:0:2:0
CIE
SEE
: 50 Marks
: 50 Marks
9) Cultivation of Anaerobes.
10) Production and estimation of Single Cell Protein.
11) Glucose uptake by Yeast and Other bacteria.
12) Ultraviolet irradiation survival curve.
13) Control of micro organism by physical and chemical factors; effects of pH, osmotic factors.
14) Isolation of Yeast and study of its characters.
Note: At least any 12 experiments must be performed
Text Books:
1. Gerhardt, P., Murray, R.G., Wood, W.A. and Kreig, N.R. (1994) Methods of General and
Molecular Bacteriology, Ed. American Society for Microbiology, Washington D.C.
2. P. M. Rhodes and P. F. Stanbury (1997) Applied Microbial Physiology : A Practical Approach,
IRL Press
Reference books:
1. Cappuccino J.G, Sherman N (1999) Microbiology: A Laboratory Manual, 4th Edn., AddisonWesley International Student.
2. Prescott, Harley and Klein (2008) Laboratory Exercises in Microbiology, 7th Ed Harley, McGrawHill, USA
3. Pollack RA, Walter F, Mondschein W, Modesto R (2004) Laboratory Exercises in Microbiology,
2nd Edn.John Wiley Publication.
Course Delivery: Regular black board teaching and interaction through Laboratory sessions
Indirect
Assessment
Methods
CIE
SEE
Students
Standard
examination
End of course
survey
Students
Max
mark
s
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
Viva- Oral
questions
Record &
Observation
book
Continuous
10
1,2,3&4
Continuous
10
End of course
50
Answer
scripts
1,2,3&4
End of course
Questionnaire
1,2,3&4
1,2,3
Questions for CIE and SEE will be designed to evaluate the various educational
components
SL NO. Blooms Category
IA
Semester-End Exam
1
Remember
15
10
26
2
3
4
5
6
Understand
Apply
Analyze
Evaluate
Create
15
20
25
5
20
25
25
15
5
Course Outcome: On completion of this course student will have improved ability:
1. To design, execute, and analyze experiments independently.
2. To apply the microbial isolation and growth technique to novel/unknown microbes.
3. To design experiments to monitor the pollution levels of contaminated water.
4. To redesign the lab experiments to extend them into research projects.
Mapping of course outcome with program outcomes
Course Outcomes
1
A
X
b
X
Program Outcome
d
E
f
g
h
X
X
i
X
j
X
X
X
PRACTICAL - II
Sub Code
Credits
: MBT108L
: 0:2:0:0
CIE
SEE
: 50 Marks
: 50 Marks
Indirect
Assessmen
t Methods
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
10
Attendance
1,2,3&4
10
Record
1,2,3&4
End of course
50
Answer
scripts
1,2,3&4
Middle of the
course
Feedback
forms
1,2,3&4
Questionnaire
1,2,3&4 and
assessment
methods
CIE
SEE
Direct Assessment
Methods
Students feedback
End of course
survey
Students
End of course
Questions for CIE and SEE will be designed to evaluate the various educational
components
SL NO.
Blooms Category
Semester-End Exam
1
Remember
20
2
Understand
20
3
Apply
35
4
Analyze
20
5
Evaluate
5
6
Create
0
28
Course Outcome: On completion of this course student will have improved ability:1. To know the importance of cloning to understand the gene transformation to bacteria
2. To understand the benefits of cell culture in the applications of genetic engineering.
3. To gain the knowledge in the production of recombinant proteins in research.
4. To know the characteristics of nucleic acids and recombinant DNA proteins in the applications
of health, medicine, industry, Food, agriculture and environment.
Mapping of course outcome with program outcomes
Course Outcomes
1
2
3
4
A
X
X
X
X
b
X
X
X
X
d
X
X
X
X
29
Program Outcome
e
f
g
X
X
X
X
X
X
h
X
X
X
X
i
X
X
X
X
j
X
X
X
X
k
X
X
X
X
BIOPROCESS ENGINEERING
Sub Code
Credit
: MBT 201
: 3:1:0:0
CIE
SEE
: 50 Marks
: 50 Marks
media for industrial fermentations, Sterilization: Batch and continuous heat sterilization-sterilization
of Liquid media, Filter sterilization of liquids. Thermal death kinetics. Bioreactors and their modes of
operations.
UNIT-5
Development of sustainable Bioprocesses:
Modeling and Assessment in Process Development- Types of Bioprocess and Biocatalysts, Raw
Materials for industrial bioprocess- Biologics and Biosimilars- Process and fermentation models for
development of processes for bioproducts and biopharmaceuticals Sustainability assessment of
Bioprocess. Process economics of bioproducts.
Text Books:
1. Pauline M Doran., Bioprocess Engineering Principles, 2nd Edition, Academic Press, USA,
2013.
2. Michael L Shuler & Fikret Kargi., Bioprocess Engineering: Basic Concepts., 2nd Edition,
Prentice Hall of India, New Delhi, 2008.
3. Elmar Heinzle, Arno P. Biwer, Charles L. Cooney. Development of Sustainable Bioprocesses
Modeling and Assessment, John Wiley & Sons Ltd, 2006.
Reference Books:
1. James E Bailey & David F Ollis., Biochemical Engineering Fundamentals, 2nd Edition,
McGraw Hill Book Co.-Singapore, 1986.
2. Tapobrata Panda., Bioreactors: Analysis and Design, 1st Edition, Tata McGraw Hill Education
Private Limited, New Delhi, 2011.
3. Douglas S. Clark, Harvey W. Blanch., Biochemical Engineering, 2nd Edition, CRC Press,
1995.
Course Delivery: Regular black Board teaching and interaction through tutorial class
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
10
Assignment
reports
1,2,3&4
10
Quiz
answers
1,2,3&4
100
Answer
scripts
1,2,3&4
In
dir
ect
As
ses
sm
ent
M
eth
od
s
Feedback
forms
1,2,3&4,
delivery of the
31
course
End of course
survey
End of course
1,2,3&4,
assessment
methods
Questionnaire
Questions for CIE and SEE will be designed to evaluate the various educational
components
SL NO.
Blooms Category
Semester-End Exam
1
Remember
30
2
Understand
30
3
Apply
15
4
Analyze
20
5
Evaluate
5
6
Create
0
Course Outcome: On completion of this course student will have improved ability to:1. Describe the steps in a catalytic mechanism and how one goes about deriving a rate law,
mechanism, and rate-limiting step that are consistent with biological experimental data.
2. Ability to understand the chemical and physical transport processes and their mechanism in
bioreactors.
3. To apply the design procedures of process equipment used in biochemical process plants to
design novel bioreactors.
4. To apply and analyze metabolic engineering concepts in industrial bioprocess.
Mapping of course outcome with program outcomes
Course Outcomes
1
2
3
4
a
X
X
X
X
b
X
X
X
X
Program Outcome
d
e
f
g
X
X
X
X
X
X
X
X
X
X
X
X
X
: MBT 202
: 3:1:0:0
CIE
SEE
: 50 Marks
: 50 Marks
32
Internal
assessment
tests
CIE
Direct Assessment
Methods
What
Class-room
open book
assignment
Surprise
Test
Students
When/ Where
(Frequency in
the course)
Thrice(Average
of the best two
will be
computed)
Twice( Average
of the two will
be computed)
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
10
Assignment
reports
1,2,3&4
Once
10
Quiz
answers
1,2,3&4
33
SEE
Standard
examination
End of course
(Answering 5
of 10 questions)
100
Answer
scripts
Middle of the
course
Feedback
forms
End of course
Questionnaire
Indirect
Assessment
Methods
Students
feedback
Students
End of course
survey
1,2,3&4
1,2,3&4,
delivery of the
course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various educational
components
ASSESSMENT PATTERN:
SL NO.
Blooms Category
1
2
3
4
5
6
Remember
Understand
Apply
Analyze
Evaluate
Create
25
25
25
15
10
0
i
X
j
X
X
X
X
BIOPHARMACEUTICAL TECHNOLOGY
Sub Code
Credit
: MBT 203
: 3:1:0:0
CIE
SEE
: 50 Marks
: 50 Marks
UNIT-1
Drug development process of protein based therapeutics: Transforming New Molecular Entities
into Drugs, Differences between Development of Biotechnology Products of Macromolecules and
Chemical Products, Current Trends in Drug Development, Drug designing: Rational, combinatorial
and High Throughput screening.
UNIT-2
Immuno-pharmacology: Overview to immunopharmacology, Antibody-mediated response,
Vaccines, Cell mediated immune response, Cancer immunotherapy, Immunosuppressant and
immunostimulators.
UNIT-3
Biotherapeutics: Hematopoietic Growth Factors and Coagulation Factors, Interferons and Cytokines
for Anti-infective and Cancer Therapy, Hormones, Enzymes, Antibodies and Derivatives.
UNIT-4
Nanotechnology for the delivery of proteins and nucleic acids based therapeutics: Introduction to
Nanotechnology in drug deliver, Nano-sized Advanced Delivery Systems as Parenteral formulation
Strategies for Hydrophobic Anti-cancer Drugs, Engineering of Amphiphilic Block Copolymers for
Drug and Gene Delivery, Nanoemulsions for Intravenous Drug Delivery. Nanotechnology for Cancer
Chemotherapy, Nanotechnology for Cancer Vaccine Delivery.
UNIT 5
Formulation of proteins and peptides: Making Small Protein Particles, Lyophilization, Multiphase
Drug Delivery Systems, Protein Compaction, Self-Emulsifying Drug Delivery Systems, skin and
parental drug delivery system.
Text books:
1. Christine M. Bladon (2002) Pharmaceutical Chemistry, John Wiley & Sons, Ltd.
2. Manfred E. Wolff (2000) Burgers Medicinal Chemistry and Drug Discovery (5th edition) A
Wiley & Sons, Inc.
3. Grietje Molema and Dirk KF. Meije (2002) Drug Targeting Organ-Specific Strategies r. WileyVCH.
4. Melgardt M. de Villiers (2007) Nanotechnology in Drug Delivery, Springer.
Reference Books:
1. Rodney JY, Milo Gibaldi (2003) Biotechnology and Biopharmaceuticals transforming proteins
and genes into drugs, A John Wiley & Sons, Inc., Publication.
2. Gavin Brooks (1998) Biotechnology in Healthcare, An introduction to biopharmaceuticals,
Pharmaceutical Press (London).
3. Shayne cox gad ( 2007) Handbook of pharmaceutical Biotechnology A John Wiley & Sons,
Inc., Publication
4. Grietje Molema and Dirk KF (2002) Drug Targeting Organ-Specific Strategies by Meijer.
Wiley-VCH.
35
Course Delivery: Regular black Board teaching and interaction through tutorial class
Indirect
Assessment
Methods
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
10
Assignment
reports
1,2,3&4
10
Quiz
answers
1,2,3&4
End of course
(Answering 5 of
10 questions)
100
Answer
scripts
1,2,3&4
Middle of the
course
Feedback
forms
End of course
Questionnaire
CIE
Classroom
Internal
open
assessm
book
ent tests
assign
ment
SEE
Assess
ment and Evaluation Vis--vis Course outcome
What
To whom When/ Where
(Frequency in
the course)
Thrice(Average
of the best two
will be
computed)
Twice( Average
of the two will
be computed)
Students
Surprise
Once
Test
Standard
examination
Students
feedback
Students
End of course
survey
1,2,3&4,
delivery of the
course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various educational
components
SL NO.
1
2
3
4
5
6
Blooms Category
Remember
Understand
Apply
Analyze
Evaluate
Create
Semester-End Exam
30
30
15
20
5
0
Course Outcome: On completion of this course student will have improved ability to:
1. To Impart principle aspects of pharmaceutical biotechnology in research and development
existing and novel biotherapeutic proteins.
2. Understanding of basic mode of protein based drug formulation
and development by
understanding the physico-chemical and pharmacological properties of drugs.
3. To apply the recent trends of nanotechnology in production, research- development of nanomedicines.
4. Understanding the pre-requisite basic information required to work in biopharmaceutical or
clinical research organization
Mapping of course outcome with program outcomes
Course Outcomes
d
36
Program Outcome
e
f
g
1
2
3
4
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
x
X
X
X
PRACTICALS - III
Sub Code
Credits
: MBT 207L
: 0:0:2:0
CIE
SEE
: 50 Marks
: 50 Marks
Direct
Assessm
ent
Method
s
CIE
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
10
Attendance
1,2,3&4
Assesment
Record
10
Record
1,2,3&4
Standard
examination
End of course
50
Answer
scripts
1,2,3&4
Students feedback
Middle of the
course
Feedback
forms
1,2,3&4
Questionnaire
1,2,3&4 and
assessment
methods
Indirect
Assessmen
t Methods
SEE
Continuous
End of course
survey
Students
End of course
Questions for CIE and SEE will be designed to evaluate the various educational
components
SL NO.
Blooms Category
Semester-End Exam
1
Remember
20
2
Understand
20
3
Apply
35
4
Analyze
20
5
Evaluate
5
6
Create
0
Course Outcome: On completion of this course student will have improved ability:1. To learn the biodiesel production
2. To understand the parameters of biodiesel and its a
3. To purify the samples with membranes in cross-flow filtration and analysis of the seperation by
adsorption.
4. To quantify the phytochemicals from plant sources and to develop the callus from suspension
culture
Mapping of course outcome with program outcomes
Course Outcomes
1
2
3
4
A
X
X
X
X
b
X
X
X
X
Program Outcome
d
e
f
g
h
X
X
X
X
X
X
X
i
X
X
X
X
j
X
X
X
X
k
X
X
X
X
PRACTICALS - IV
Sub Code
Credits
: MBT 208L
: 0:0:2:0
CIE
SEE
: 50 Marks
: 50 Marks
CIE
SEE
Direct Assessment
Methods
End of course
39
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
10
Attendance
1,2,3&4
10
Record
1,2,3&4
50
Answer
scripts
1,2,3&4
Indirect
Assessmen
t Methods
Students feedback
End of course
survey
Middle of the
course
Feedback
forms
1,2,3&4
End of course
Questionnaire
1,2,3&4 and
assessment
methods
Students
Questions for CIE and SEE will be designed to evaluate the various educational
components
SL NO.
Blooms Category
Semester-End Exam
1
Remember
20
2
Understand
20
3
Apply
35
4
Analyze
20
5
Evaluate
5
6
Create
0
Course Outcome: On completion of this course student will have improved ability:1. Students will be capable to perform important quality control tests to validate quality of
product.
2. Able to standardize the therapeutic compounds.
3. Competent to work in Pharmaceutical /Clinical research organization.
4. Improving the research aptitude of students by imparting the knowledge of nanotechnology
and advanced drug delivery system
Mapping of course outcome with program outcomes
Course Outcomes
1
2
3
4
A
X
X
X
X
b
X
X
X
X
40
Program Outcome
d
e
f
g
h
X
X
X
X
X
X
X
i
X
X
X
X
j
X
X
X
X
k
X
X
X
X
: MBT 301
: 3:1:0:0
CIE
SEE
: 50 Marks
: 50 Marks
Textbooks:
1. Sateesh M.K (2008) Bioethics & Biosafety, IK Publishers.
2. Traynor PL (2000) Biosafety Management, Virginia polytechnic Institute Publication.
3. N K Acharya (2007) Text book on Intellectual Property Rights, Asia Law house, 4th edn.
Reference Books:
1. Sasson A (1993) Biotechnologies in developing countries present and future, UNESCO Publishers.
2. Rao MB (2003) WTO and International Trade, Vikas Publishing House Pvt. Ltd.
3. Erbisch FH and Maredia KM (2003) Intellectual Property Rights in Agricultural Biotechnology,
Orient Longman Ltd.
4. Deborah E Bouchoux (2005) Intellectual Property Rights, Delmar Cengage learning
5. Patent Fundamentals for Scientists and Engineers, Thomas T Gordon and Arthur S Cookfair, CRC
Press (1995).
Course Delivery: Regular black Board teaching, Using teaching aids like power point presentation
and interaction through tutorial class
Indirect
Assessment
Methods
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
Thrice(Average
of the best two
will be
computed)
30
Blue books
1,2,3&4
Assignment
Once
10
Assignment
reports
1&2
CIE
Internal
assessment
tests
Students
Mini Project
SEE
Standard
examination
Once
10
End of course
(Answering 5 of
10 questions)
100
Answer
scripts
1,2,3&4
Middle of the
course
Feedback
forms
1&2
End of course
Questionnaire
1,2,3&4
Students feedback
End of course
survey
Mini Project
reports
Students
3&4
Questions for CIE and SEE will be designed to evaluate the various educational
components
S.No
1
2
Blooms Category
Remember
Understand
Test 1
10
30
Test 2
10
30
42
Test 3
10
30
Semester-End Examination
10
30
3
4
5
6
Apply
Analyze
Evaluate
Create
30
30
0
0
30
30
0
0
30
30
0
0
30
30
0
0
NANO-BIOTECHNOLOGY
Sub Code
Credits
: MBTE01
: 4:0:0:0
CIE
SEE
: 50 Marks
: 50 Marks
beam writing, and scanning probe patterning). Biological Methods: Microbial production of inorganic
nanoparticles Magnetosomes .DNA based nanostructures
UNIT-3
Characterization of Nanomaterials : Structural Characterization: X-ray diffraction, Small angle Xray Scattering, Optical Microscope and their description, Scanning Electron Microscopy (SEM),
Scanning Probe Microscopy (SPM), , Scanning Tunneling Microscopy (STM), Atomic force
Microscopy (AFM). Spectroscopic characterizations: application of UV-VIS-IR Raman spectroscopy
for analysis of nanomaterials, Surface Characterization: X-ray Photoelectron Spectroscopy (XPS),
Auger electron spectroscopy, Low Energy Ion, Scattering Spectroscopy (LEISS), Secondary Ion Mass
Spectroscopy (SIMS), Rutherford Backscattering Spectroscopy (RBS). Resonance Methods: Electron
Spin Resonance (ESR), Ferromagnetic Resonance (FMR), Nuclear Magnetic Resonance (NMR),
Mossbauer Spectroscopy.
UNIT 4
Biological Nano-materials: Protein based nanostructures building blocks and templates Proteins as
transducers and amplifiers of biomolecular recognition events Nanobioelectronic devices and
polymer nanocontainers. DNA based nanostructures Topographic and Electrostatic properties of
DNA and proteins Hybrid conjugates of gold nanoparticles DNA oligomers Use of DNA
molecules in nanomechanics and Computing. Nano diamonds. Biocompatable polymers: liposomes,
dendrimers, chitosan
UNIT 5
Biological Application of Nanotechnology: Nanoparticles in Therapeutic applications Drug
delivery, imaging and cancer treatment, bone substitutes and dentistry, Implants and Prosthesis,
Reconstructive Intervention and Surgery, Nanorobotics in Surgery, Photodynamic Therapy, Neuroelectronic Interfaces, Protein Engineering. Nanotechnology in Agriculture and Food Technology,
Biosensors: Principles- DNA based biosensors Protein based biosensors, Nanosensors in Diagnosis.
DNA Templated Electronics, Sequence specific molecular lithography, Single Biomolecule.
Manipulation for Bioelectronics, DNA as a semiconductor. Environmental issues, toxicity of
nanomaterials., ethical issues, the future of nanotechnology in medicine.
Text books:
1. Edelstein A.S, Cammaratra R.C (1996) Nanomaterials: Synthesis, Properties and Applications,
Second Edition, CRC PressTaylor and Francis group New York USA
2. Christof M. Niemeyer, Chad A. Mirkin (2004)Nanobiotechnology: Concepts, Applications and
Perspectives John Wiley & Sons
3. Yubing Xie (2012)The Nanobiotechnology Handbook CRC Press Taylor and Francis group
New York USA
Reference Books:
1. Richard Booker and Earl Boysen (2005) Nanotechnology, Wiley Dreamtech.
2. Chapman & Hall (2002) NanobiotechnologyBasic Science & Emerging Technologies, CRC
Press.
3. Eric K Drexler, Pelerson C, Pergamit G (1993) Unbounding the future. William Marrow and
Company
4. Mark Ratner and Daniel Ratner (2005) Nanotechnology. Prentice Hall
5. Murthy DVS (1995) Transducers and instrtumentation. Prentice Hall of India
6. Jing chung & Larry J. Kricka (2001) Biochip Technology. Harwood academic publishers.
Course Delivery: Regular black Board teaching and interaction through tutorial class
44
Indirect
Assessment
Methods
SEE
CIE
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
10
Assignment
reports
1,2,3&4
Surprise Test
Once
10
Quiz
answers
1,2,3&4
Standard
examination
End of course
(Answering 5 of 10
questions)
100
Answer
scripts
1,2,3&4
Middle of the
course
Feedback
forms
End of course
Questionnaire
Students feedback
Students
End of course
survey
1,2,3&4,
delivery of the
course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various educational
components
S.No
1
2
3
4
5
6
Blooms Category
Remember
Understand
Apply
Analyze
Evaluate
Create
Test 1
20
30
30
10
10
0
Test 2
20
30
30
10
10
0
Test 3
20
30
30
10
10
0
Semester-End Examination
20
30
30
10
10
0
Course Outcome: On completion of this course student will have the improved ability to:1) Develop an understanding of the fundamental concepts in nanotechnology.
2) Relate to current nanotechnological applications in the field of biotechnology.
3) Think of novel, future applications of nanotechnology in biotechnology and for molecular
medicine.
4) Have knowledge in Applications of Nano-Drug Delivery, Diagnostics and Nanotherapeutics.
Mapping of course outcome with program outcomes
Program Outcome
Course
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12
Outcomes
1
X
X
X
X
X
X
X
X
X
2
X
X
X
X
X
X
X
X
3
X
X
X
X
X
X
X
4
X
X
X
X
X
X
X
Sub Code
Credits
: MBTE 02
: 4:0:0:0
CIE
SEE
: 50 Marks
: 50 Marks
Course Delivery: Regular black Board teaching and interaction through tutorial class
Indirect
Assessment
Methods
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
10
Assignment
reports
1,2,3&4
10
Quiz
answers
1,2,3&4
100
Answer
scripts
1,2,3&4
Middle of the
course
Feedback
forms
End of course
Questionnaire
CIE
Classroom
Internal
open
assessm
book
ent tests
assign
ment
SEE
Standard
examination
Students feedback
Students
End of course
survey
1,2,3&4,
delivery of the
course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various educational
components
S.No
1
2
3
4
5
6
Blooms Category
Remember
Understand
Apply
Analyze
Evaluate
Create
Test 1
20
30
30
10
10
0
Test 2
20
30
30
10
10
0
Test 3
20
30
30
10
10
0
Semester-End Examination
20
30
30
10
10
0
Course Outcome: On completion of this course student will have the improved ability to:1. Use the basic and applied knowledge gained through other courses in biotechnology to relate to
nutraceuticals and value addition of foods.
2. Understand various methods of value addition of foods.
3. Perceive the expected benefits of value addition.
4. Learn to use genetic engineering to modify and manipulate biological processes for value
addition of foods.
Mapping of course outcome with program outcomes
Program Outcome
Course
PO1
PO2
PO3
PO4
PO5
PO6 PO7 PO8 PO9 PO10 PO11 PO12
Outcomes
1
X
X
X
X
X
X
2
X
X
X
X
X
X
3
X
X
X
X
X
X
X
47
MEDICAL BIOTECHNOLOGY
Sub Code
: MBTE 03
CIE
Credit
: 4:0:0:0
SEE
Course coordinators: Dr. Prabha, M. and Mr. Lokesh, K.N.
: 50 Marks
: 50 Marks
Text Books:
1. Daan Crommelin, Robert D Sindelar and Bernd Meibohm (2007). Pharmaceutical
Biotechnology and Fundamental Applications, 2nd edition. Informa Health care USA, Inc.
2. Willam Irving, Time Boswell and Dlawar AlaAldeen (2006) BIOS Instant notes in Medical
Microbiology. BIOS Scientific Publication.
3. Sambamurthy K and Ashutosh Kar (2006) Text book of Pharmaceutical Biotechnology,
Paperback 1st edn. New Age International.
Reference Books:
1. Judit Pongracz and Mary Keen (2009) Medical Biotechnology, Churchill Livingstone
publication.
2. Albert Sasson (2006) Medical Biotechnology, Brookings Institution Press.
3. Bernhard O Palsson and Sangeeta N Bhatia (2003) Tissue Engineering, Pearson Prentice Hall.
4. Pamela Greenwell, Michelle McCulley, Molecular Therapeutics: 21st century medicine, 1st
Edition.
5. Lela Buchingham and Maribeth L Flawsm, Molecular Diagnostics: Fundamentals, Methods and
Clinical Applications, 1st Edition, F A Davis Company, Philadelphia, USA, 2007.
Course Delivery: Regular black Board teaching and interaction through tutorial class
Indirect
Assessment
Methods
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
10
Assignment
reports
1,2,3&4
10
Quiz
answers
1,2,3&4
End of course
(Answering 5 of
10 questions)
100
Answer
scripts
1,2,3&4
Middle of the
course
Feedback
forms
End of course
Questionnaire
CIE
SEE
1,2,3&4,
delivery of the
course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various educational
components
SL NO.
Blooms Category
Semester-End Exam
1
Remember
25
49
2
3
4
5
6
Understand
Apply
Analyze
Evaluate
Create
30
20
20
5
0
Course Outcome: On completion of this course student will have improved ability:1. To have the knowledge on disease causing microbes, diagnosis, prevention of diseases and
treatment
2. To understand the benefit and scope of Embryonic stem cells, Hemopoietic stem cells and their
applications for the transplantation in treatment of cancer and other various diseases.
3. To learn the cell, tissue engineering techniques, nanomedicine and its applications in different
areas of medicine.
4. To understand the Clinical applications of recombinant technology in gene therapy for the
treatment of genetic and other diseases with clinical studies and pharmaceutical production.
Mapping of course outcome with program outcomes
Course Outcomes
1
2
3
4
a
X
X
X
X
b
X
X
X
X
d
X
X
X
X
Program Outcome
e
f
g
X
X
X
X
X
X
X
X
X
X
X
X
h
X
X
X
X
i
X
X
X
j
X
X
X
X
k
X
X
X
X
BIOREACTION ENGINEERING
Sub Code
Credit
: MBTE 04
: 4:0:0:0
CIE
SEE
: 50 Marks
: 50 Marks
Media Design and Sterilization for Fermentation Process: Medium requirements for fermentation
process-examples of simple and complex media; Design and usage of commercial media for industrial
fermentations, Thermal death kinetics of microorganisms, Batch and continuous heat-sterilization of
Liquid media, Filter sterilization of liquids
UNIT-3
Transport Phenomena in Bioreactors: Mass transfer in heterogeneous biochemical reaction
systems, Oxygen transfer in submerged fermentation process, Oxygen uptake rate and determination
of oxygen transfer coefficients (kLa), role of aeration and agitation in oxygen transfer, Heat transfer
process in biological systems
UNIT-4
Mechanical Design of Bioreactors and Ancillary Equipments: Basic design and construction of
fermenters and its ancillaries; Material of construction, Vessel geometry, Bearing assemblies, Motor
drives, Aseptic seals, Flow measuring devices, Valves, Agitator and Spurges Design, Sensor.
UNIT-5
Process Design and Operation Bioreactors: Operational models of reactors, Batch continuous, Fed
Batch, repetitive batch, recycle and continuous cultivation, novel bioreactors, stirred tank, Air lift and
loop reactors, Packed bed and Hollow fiber membrane bioreactors, Bioreactors for waste treatment
processes; Scale-up of bioreactors, SSF bioreactors.
Text Books:
1. Bailey and Ollis (2010): Biochemical Engineering Fundamentals, McGraw Hill (2nd Ed)
2. Schuler ML and Kargi F (2002): Bioprocess Engineering- Basic concepts by, Prentice Hall (2nd
Ed)
3. Levenspiel (2006) Chemical reaction Engineering, 3rd ed., John Wiley.
Reference Books:
1. Ghose TK (Ed)(1994) Process computation in Biotechnology Tata Mc-Craw hill
2. Athinson B and Maviuna F (1993) Biochemical Engg. And Bitoechnology Handbook, Mc-Graw
hill (2nd Edition)
3. Pauline M. Doran (2009):Bioprocess Engineering Principles, Reed Elsevier India.
4. Fermentation & Biochemical Engineering Hand book (1996) Principles Process Design and
Equipment, HC Vogel, Noyes.
Course Delivery: Regular black Board teaching and interaction through tutorial class
CIE
Direct Assessment
Methods
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
10
Assignment
reports
1,2,3&4
10
Quiz
answers
1,2,3&4
SEE
Indirect
Assessment
Methods
Standard
examination
Students feedback
End of course
(Answering 5 of
10 questions)
100
Answer
scripts
Middle of the
course
Feedback
forms
End of course
Questionnaire
1,2,3&4
1,2,3&4,
delivery of the
course
1,2,3&4,
assessment
methods
Students
End of course
survey
Questions for CIE and SEE will be designed to evaluate the various educational
components
SL NO.
Blooms Category
Semester-End Exam
1
Remember
30
2
Understand
30
3
Apply
15
4
Analyze
20
5
Evaluate
5
6
Create
0
Course Outcome: On completion of this course student will have improved ability:1. Describe the algorithm that allows the student to solve chemical reaction engineering problems
through logic rather than memorization.
2. Size isothermal and non-isothermal reactors for homogeneous and heterogeneous reactions.
3. Analyze multiple reactions carried out both isothermally and non-isothermally in flow, batch
and semi batch reactors to determine selectivity and yield.
4. Determine the reaction order and specific reaction rate from experimental data.
Mapping of course outcome with program outcomes
Course Outcomes
1
2
3
4
a
X
X
X
X
b
X
X
X
X
Program Outcome
e
f
g
X
X
X
X
X
X
X
X
X
d
X
X
X
X
k
X
: MBTE 05
: 4:0:0:0
CIE
SEE
: 50 Marks
: 50 Marks
3. Understand the effects of different toxicants and stressors in terms of target effect on the
cellular-, organ system- and whole body-levels.
4. Aware of general principles of environmental, occupational and preventive aspects of
toxicology.
Unit 1
Introduction to toxicology: History and scope of toxicology, Source of toxicants. Classification of
toxic agents. Occupational toxicology: Workplace, hazardous exposure, and occupational diseases.
Mechanism of toxicity: Toxicant delivery, reaction with the target molecule, cellular dysfunction,
inappropriate repair and adaptation. Non target organ toxicity: Chemical carcinogenesis mechanisms of
carcinogens. Genetic toxicology mechanisms of genetic alterations. Teratology: teratogens,
teratogenesis. Cytotoxicity mechanisms of cell death mitochondrial dysfunction,
Unit 2
Target organ toxicity and metabolism of toxicants: Toxic effects on liver, kidney, nervous,
endocrine, respiratory, immune and reproductive systems. Metabolism of toxicants: Phase I Reactions:
Microsomal oxidation Nonmicrosomal oxidations Reduction Reactions, Hydrolysis, Epoxide
Hydration. cooxidation. Phase II Reactions: Conjugation reactions, Methyltransferases and Acylation.
Reactive Metabolites: nature, stability and fate of reactive metabolites, Elimination of Toxicants: renal,
hepatic and respiratory elimination
Unit 3
Toxicology Testing: Food toxicology: introduction, safety standards for foods and food ingredients
and contaminants. In Vivo Toxicology: Testing of acute, subchronic and chronic toxicity. In Vitro
testing: Cell Culture Methods, Ames forward mutation assay, Assessing genotoxicity: mitotic index,
chromosomal aberrations, micronucleus assay, cytotoxicity and apoptosis assay. Neurotoxicity testing.
Unit 4
Introduction to Forensic science: Introduction, Definition and Scope, History and Development of
Forensic science, basic Principles of Forensic Science. Organization of crime Laboratory services,
services provided by full service crime laboratories, Physical Science unit, Biological Unit, Firearms
Unit, Documentation Examination Unit- Function and Duties Performed by each unit and lab. The
Crime Scene investigation- Making and recording observations (including sketches with
measurements and digital photographs), Chain of Custody, Locard Exchange principle, Evidences and
Collection techniques, Firearms, Marks and impressions, Drug of abuse. Ploygraphy. Computer
Forensics.
Unit 5
Forensic Biology: Forensic Pathology: Rigor mortis, Lovor mortis, Algor mortis. Forensic
Anthropology, Forensic Entomology, Forensic Psychiatry, Forensic Odontology, Forensics
Engineering, forensic serology, DNA Analysis, Dactyloscopy, Finger prints: history, fundamental
principle of Fingerprints, Classification and patterns, AFIS, Mrthod of Detecting fingerprint. Trace
evidence and contact evidence- targeting potential traces, recovery of trace material assessment of
significance- Hair, fiber and Paint.
Textbooks:
1. Barile FA (2008) principles of toxicology testing CRC Press is an imprint of the Taylor &
Francis Group New York
2. Hodgson E (2004) A Textbook of Modern Toxicology Third edition John wiley & sons, inc.,
publication
53
3. Curtis D. Klaassen (2001) Casarett and Doulls Toxicology The Basic Science Of Poisons
Sixth Edition Mcgraw-Hill publishers New Delhi
Reference books
1. Osweiler GD (1996) Toxicology, Wiley-Blackwell Publisher,
2. Marquardt H (1999) Toxicology, Academic Press
3. Derelanko MJ (2002) Handbook of toxicology, CRC Press,
Course Delivery: Regular black Board teaching and interaction through tutorial class
Indirect
Assessment
Methods
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
10
Assignment
reports
1,2,3&4
10
Quiz
answers
1,2,3&4
End of course
(Answering 5 of
10 questions)
100
Answer
scripts
1,2,3&4
Middle of the
course
Feedback
forms
End of course
Questionnaire
CIE
SEE
Students feedback
Students
End of course
survey
1,2,3&4,
delivery of the
course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various educational
components
SL NO.
Blooms Category
Semester-End Exam
1
Remember
20
2
Understand
25
3
Apply
30
4
Analyze
20
5
Evaluate
5
6
Create
0
Course Outcome: On completion of this course student will have improved ability:1. To understand the basic aspects of toxicology and forensic science; the key areas, the
conventions followed and the scope of toxicology and forensic science.
2. To explain how toxicants get into the environment and which diseases may be associated with
them.
3. To identify the damage process, action and metabolism of toxic chemicals.
4. To describe how toxic chemicals are tested and regulated.
54
A
X
X
X
X
b
X
X
X
X
Program Outcome
e
f
g
X
X
X
X
X
X
X
X
X
X
X
d
h
X
X
X
X
i
X
X
X
x
PLANT BIOTECHNOLOGY
Sub Code
Credit
: MBTE 06
: 4:0:0:0
CIE
SEE
: 50 Marks
: 50 Marks
Introduction: Introduction and historical developments and applications of Plant tissue and cell
culture. Laboratory Design and Developments. Instrumentation. Sterilization techniques, Plant Tissue
Culture Media, Cellular totipotency, Factors affecting Tissue Culture success: (Media explant, light,
Temperature, Polarity, Subculture, Genotype, Season), Hormones.
UNIT-II
Plant Tissue and cell culture: Micropropagation, organ culture, Establishing callus and cell culture,
Dynamics of callus growth, callus subculture and maintenance, organogenesis. Embryogenesis, variant
selection, Somaclonol variation, cell suspension culture, Somatic embryogenesis in plant. Protoplast
isolation and culture. Acclimatization of micro propagated plant. Hairy root culture and its importance,
Germplasm storage.
UNIT-III
Molecular genetics of gene expression: Structure and organization of plant genome, regulation of
plant genome expression, transcriptional, translational regulation of plant genome. Recombinant DNA,
vector design, Marker gene and promoters. Gene and traits of the interest for transgenic plants.
Amplification of DNAs by Polymerase Chain Reaction (PCR). Marker assisted selection (RAPD,
RFLP, AFLP, SNP`s etc.).
UNIT-IV
Transgenic plant production: Transposons, Gene transfer technology Vectors, Gene transfer using
Particles Bombardment, Microinjection method, Transfer of DNA to plant cells- Direct transformation
55
by electroporation and particle gun bombardment. Agrobacterium, Ti plasmid vector Theory and
techniques for the development of new genetic traits. Transgenic plant analysis PCR, ELISA and
phenotypic analysis.
UNIT-V
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
10
Assignment
reports
1,2,3&4
10
Quiz
answers
1,2,3&4
100
Answer
scripts
1,2,3&4
Middle of the
course
Feedback
forms
End of course
Questionnaire
Indirect
Assessmen
t Methods
SEE
CIE
Standard
examination
Students feedback
Students
End of course
survey
56
1,2,3&4,
delivery of the
course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various educational
components
SL NO.
Blooms Category
Semester-End Exam
1
Remember
30
2
Understand
30
3
Apply
20
4
Analyze
20
5
Evaluate
0
6
Create
0
Course Outcome: On completion of this course student will have improved ability:1. Able to learn the concept of plant tissue culture and its importance.
2. Able to prepare media formulation, optimization and design of experiments for development of
new bioprocess protocols.
3. Able to discuss the significance of plant tissue culture to the nursery and agricultural crop
industries;
4. Identify and apply basic plant growth theories to plant tissue culture techniques.
Mapping of course outcome with program outcomes
Course Outcomes
1
2
3
4
a
X
X
X
X
X
X
Program Outcome
d
e
f
g
X
X
X
X
X
EXPERIMENTAL DESIGN
Sub Code
Credits
: MBTE 07
: 4:0:0:0
CIE
SEE
: 50 Marks
: 50 Marks
Correlation, Regression analysis, Partial and multiple correlation and regression, Regression
diagnostics - residuals, multicollinearity, testing adequacy of fit and validation in regression analysis;
Sampling distribution of correlation and regression coefficients; Testing of hypothesis: z test, t-test,
Chi-square test and F-test
UNIT-3
Joint probability distributions for discrete and continuous variables, conditional expectation, stochastic
independence, {generalization to n dimensional random variables}
Concept of analysis of variance and covariance of data for one-way and multi-classified experiments;
Multiple Mean Comparison, Pair wise Contrasts, Type of Models (Fixed or Random), Incomplete
Blocks/Missing data
UNIT-4
Fishers principles, Basics of designing an experiment Completely Randomized Design (CRD),
Principles and Usage, Randomization, Data Analysis/Model (one-way ANOVA),
Advantages/Disadvantages, Ideal Conditions (assumptions)/Data transformation, Randomized
Complete Block Design, One-way Blocking, Data Analysis (multi-way ANOVA), Data Analysis
(multi-way ANOVA)
UNIT-5
Latin squares: The 2k factor design, Blocking and confounding, two level fractional factorial design,
Three level and mixed level factorial, fractional design and Plackett- Burman design.
Factorial Treatment Structure, Two level factorial designs, Blocking and confounding systems for two
level factorials, Partial confounding, Fractional factorial design, Yates forward algorithm, Choice of
treatments, Qualitative/Quantitative factors.
Review of Linear Regression Analysis, Analysis of Covariance (ANCOVA), Blocks and / or
Covariates
Test books:
1. Misra BL (2005) Design & Analysis of Experiments for Agricultural Workers, 1st Ed, Kalyan Pub.
2. Douglas C. Montgomery (2009) Design and Analysis of Experiments, 7th Edition, Wiley
publication.
3. Rudra Pratap (2005) Getting started with Mat lab, Oxford University Press.
4. Arun Kumar and Alka Chaudary (2006) Sample Survey Analysis and Design of Experiment, 2 nd
Edition, Krishna Prakash Media (P) Ltd.
Reference Books:
1. Sundararaj N, Nagaraju S, Ramu MNV, Jagannath MK (1972) Design and analysis of field
experiments. Miscellaneous Series, University of Agricultural Sciences, Bangalore.
2. Neural
Network
Toolbox
6
User's
Guide,
Matlab
Inc,
USA,
Source:
http://www.mathworks.com/access/helpdesk/help/pdf_doc/nnet/nnet.pdf
3. Genetic
algorithm
Toolbox
User's
Guide,
Matlab
Inc,
USA,
Source:
http://www.mathworks.com/access/helpdesk_r13/help/pdf_doc/gads/gads_tb.pdf
5. Steel & Torrie (1980) Principals and Procedures of Statistics A Biometrical Approach.
6. Kirk (1995) Experimental Design, Procedures for the Behavioral Sciences.
7. Kuehl (2000) Design of Experiments: Statistical Principals of Research Design and Analysis.
8. Quinn & Keough (2002) Experimental Design and Data Analysis for Biologists.
Course Delivery: Regular black Board teaching and interaction through tutorial class
Assessment and Evaluation Vis--vis Course outcome
What
To
When/ Where
58
Max
Evidence
Contributing
Indirect
Assessment
Methods
SEE
CIE
whom
(Frequency in the
course)
Thrice(Average of
the best two will be
computed)
Twice( Average of
the two will be
computed)
marks
collected
to Course
Outcomes
30
Blue books
1,2,3&4
10
Assignment
reports
1,2,3&4
Surprise Test
Once
10
Quiz
answers
1,2,3&4
Standard
examination
End of course
(Answering 5 of 10
questions)
100
Answer
scripts
1,2,3&4
Middle of the
course
Feedback
forms
End of course
Questionnaire
Internal
assessment
tests
Class-room
open book
assignment
Students
Students feedback
Students
End of course
survey
1,2,3&4,
delivery of the
course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various educational
components
SL NO.
Blooms Category
Semester-End Exam
1
Remember
40
2
Understand
20
3
Apply
20
4
Analyze
15
5
Evaluate
5
6
Create
0
Course Outcome: On completion of this course student will have improved ability:1. To understand the basic aspects of statistics and mathematical models used in biological
research.
2. To acquire working knowledge on the statistical models and their applications in the research.
3. To execute different techniques to collect, design, store and analyzing data in different fields of
research.
4. To correlate the statistical significance of observed data with that of experimental data using
software tools.
Mapping of course outcome with program outcomes
Course Outcomes
1
2
3
4
A
X
X
X
X
b
X
X
X
X
c
X
X
X
X
d
X
X
X
X
Program Outcome
e
f
g
X
X
X
X
X
X
X
X
APPLIED BIOINFORMATICS
59
h
X
X
X
X
i
X
X
X
X
Sub Code
Credit
: MBTE 08
: 4:0:0:0
CIE
SEE
: 50 Marks
: 50 Marks
Indirect
Assessment
Methods
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
10
Assignment
reports
1,2,3&4
10
Quiz
answers
1,2,3&4
End of course
(Answering 5 of
10 questions)
100
Answer
scripts
1,2,3&4
Middle of the
course
Feedback
forms
End of course
Questionnaire
CIE
SEE
Students feedback
Students
End of course
survey
1,2,3&4,
delivery of the
course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various educational
components
SL NO.
Blooms Category
Semester-End Exam
1
Remember
30
2
Understand
25
3
Apply
20
4
Analyze
20
5
Evaluate
5
6
Create
00
Course Outcome: On completion of this course student will have improved ability:61
1. Student can get the knowledge of using bioinformatics online tools, free softwares and servers
to uncover the diversity of life revealed in genomes and explore the genomic origins of life.
2. Get the knowledge of using high-throughput methods to characterize cellular roles for proteins,
utilization of structural information to discern how proteins work and incorporate structural
methods to develop better drugs.
3. Apply in silico and in vivo methods to comprehend whole proteome interactions and evaluate
methods for quantifying and comparing proteomes.
4. Get hands on experience of using online tools to analyze genome sequences.
Mapping of course outcome with program outcomes
Course Outcomes
1
2
3
4
Sub Code
Credit
A
X
X
X
X
b
X
X
X
X
X
Program Outcome
d
e
f
g
X
X
X
X
X
X
X
X
X
X
X
X
RESEARCH METHODOLOGY
: MBTE 09
CIE
: 4:0:0:0
SEE
h
X
X
X
X
X
X
X
X
X
X
X
: 50 Marks
: 50 Marks
Course coordinators:
Objectives of the course: The course will help to:
1. Understand the importance of doing systematic research and experimental designs.
2. Study various methods/techniques of conducting research.
3. Opportunity to learn different statistical methods of analysis.
4. Understand the systematic methods of presentation of research finding using modern facilities.
UNIT-1
Introduction to Research Methodology: definition and objectives. Types of research: descriptive
research, experimental method of research, inter and multi disciplinary research. Design of research:
basic principles of experimental designs, features of good research design, types of designs.
Literature search & formulation of research project.
UNIT-2
Different techniques of research: observation, the interview, the questionnaire & the case study
method. Survey methods and sampling techniques: sampling design, random sample and complex
random sample design. Data collection: collection of primary and secondary data.
UNIT-3
Basic statistical methods, concepts and techniques: Processing and analysis of data-types of
analysis, measure of dispersion; Correlation: Simple, partial and multiple correlations. Regression:
linear and non liner regression.
UNIT-4
Research report/paper writing: types of reports, steps in report writing, meaning and techniques
of interpretations. Dissertation/ Thesis writing: Introduction, review of literature, materials and
methods, experimental results, discussion/ interpretation of results in the light of earlier research
findings, summary and bibliography.
62
UNIT-5
Computers its application in research: Computer Skills: Spread sheet and DBMS. Graphicshistograms, line diagrams, bar diagrams and Pie charts. Statistical analysis using computer packages:
Design Expert/Statistica /Minitab softwares, SPSS
Text Books:
1. Debbie Holmes, Peter Moody, and Diana Dines (2006) Research Methods for the Biosciences,
2nd Edition,
2. Oxford University Press Inc., New York.
3. Kothari, C.R. (2002) Research Methodology, 7th Print, 2nd Edition, New Age International,
Bangalore.
Reference Books:
1. Suresh C. Sinha and Anil K. Dhiman (2002) Research Methodology, 2 volumes, Ess. Ess.
Publishers, New Delhi,
2. Kumar (2008) Research Methodology, 7th Edn, Lakshmi Narayan Agarwal, Agra, India
3. Panneerselvam, R. (2004) Research Methodology, 1st Edition, Prentice-Hall of India Pvt. Ltd.,
New Delhi. Imbibe
Course Delivery: Regular black Board teaching and interaction through tutorial class
Indirect
Assessment
Methods
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
10
Assignment
reports
1,2,3&4
10
Quiz
answers
1,2,3&4
100
Answer
scripts
1,2,3&4
Middle of the
course
Feedback
forms
End of course
Questionnaire
CIE
SEE
Standard
examination
Students
feedback
Students
End of course
survey
1,2,3&4,
delivery of the
course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various educational
components
SL NO.
Blooms Category
Semester-End Exam
1
Remember
10
2
Understand
15
63
3
4
5
6
Apply
Analyze
Evaluate
Create
25
20
20
10
Course Outcome: On completion of this course student will have improved ability:1. To compare and use various experimental methods of conducting experiments.
2. To Understand and appreciate various techniques of research based on situation.
3. To plan and design the experiment and can execute accordingly
4. To Report the research results in standard format and analyze using modern computing
facilities.
Mapping of course outcome with program outcomes
Course Outcomes
1
2
3
4
A
X
X
b
X
X
Program Outcome
e
f
g
X
X
X
X
X
X
X
X
X
h
X
X
X
i
X
X
j
X
X
k
X
X
: MBTE 10
: 4:0:0:0
CIE
SEE
: 50 Marks
: 50 Marks
IFA (membrane, cytoplasmic and nuclear proteins). Organ culture- techniques, three dimensional
culture. Somatic cell fusion and its applications (cybrids, membrane fluid mobility and hybridoma
technology).
Unit III
Artificial animal Breeding and Transgenic Technology: Artificial insemination, tissue and
organTransplantation, in vitro fertilization and embryo transfer, Advantages of cell manipulation,
Nuclear transplantation and animal cell cloning, selective animal breeding and their potential.
Production and uses of transgenic animals. Animals as a bioreactor for production various chemicals.
Application of functional genomics and discovery of new genes, animal welfare and human health.
Unit IV
Mammalian Stem cells and its application: Source and isolation of stem cells, Embryonic and adult
stem cells, stem cellculture and maintenance. Generation and manipulation of mouse and human
embryonic stem cells. Germ Cell Development: Epigenesis and Reprogramming of adult-stem cells.
Molecular mechanisms of self-renewal and differentiation, pluri/multipotency and lineage
differentiation. Bone transplant and reconstitution of hematopoietic system. Stem cells and
therapeutics. Novel sources of multipotent stem cells. Science policies and Ethics in Stem Cell
Research
Unit V
Applications of Animal Biotechnology: improvement in Animal production: diary, fishery and
poultry. Production of pharmaceutical chemicals, interferons, interleukins, stem cell factors and
hormones. Industrial applications: metabolites production, bio control agents, industrially important
enzymes. Medicine: diagnosis of diseases, detection of genetic disorders. Treatment: vaccines, gene
and cell therapy, tissue transplantations. Drug testing and evaluation.
Textbooks:
1. Freshney RI (2005) Culture of Animal Cells, 5th Edn, Wiley-Liss.
2. Spier RE and Griffiths JB (1988) Animal Cell Biotechnology, Academic Press.
3. Clynes (1998) Animal Cell Culture Techniques, 1st Edn, Springer.
Reference Books:
1. Channarayappa (2006) Molecular Biotechnology: Principles and Practices. University Press
(India) Pvt. Ltd., Worldwide CRC Press.
2. Channarayappa (2010) Cell Biology: Universities Press (India) Pvt Ltd.
2. John RW, Masters, (2000) Animal Cell Culture: Practical Approach, 3rdEdn, Oxford.
3. Murray Moo-Young (1989) Animal Biotechnology, Pergamon Press, Oxford.
4. Doyle A, Hay R, and Kirsop BE (1990) Living Resources for Biotechnology, Animal cells,
Cambridge University Press
Course Delivery: Regular black Board teaching and interaction through tutorial class
Direct
Assessm
ent
Method
s
CIE
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
10
Assignment
1,2,3&4
Indirect
Assessment
Methods
SEE
open book
assignment
Surprise
Test
Standard
examination
reports
Once
10
Quiz
answers
1,2,3&4
End of course
(Answering 5 of
10 questions)
100
Answer
scripts
1,2,3&4
Middle of the
course
Feedback
forms
End of course
Questionnaire
Students
feedback
Students
End of course
survey
1,2,3&4,
delivery of the
course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various educational
components
SL NO.
Blooms Category
Semester-End Exam
1
Remember
20
2
Understand
30
3
Apply
30
4
Analyze
15
5
Evaluate
5
6
Create
0
Course Outcome: On completion of this course student will have improved ability:1. To understand the basic nutritional requirements of animal cell and different types of cell
culture techniques.
2. To impart knowledge on artificial breeding and production of transgenic animals.
3. To explain isolation and culturing of stem cells and their application in biomedical field.
4. To describe applications of cell culture, transgenic and stem cell culture techniques in the field
of modern life science.
Mapping of course objectives with program outcomes
Course Outcomes
1
2
3
4
a
X
X
X
X
b
X
X
X
X
Program Outcome
e
f
g
X
X
X
X
X
X
X
X
X
X
h
X
X
X
X
j
X
X
x
Reference Books:
1. Roland Glaser (2004), Biophysics: An Introduction, Springer.
2. Cantor CR and Schimmel PR (1980) Biophysical Chemistry: Part I, The conformation of
biological macromolecules.
3. Kensal Edward Van Holde, W. Curtis Johnson, Pui Shing Ho (2006), Principles of Physical
Biochemistry. Peasrson Printice Hall.
Course Delivery: Regular black Board teaching and interaction through tutorial class
CIE
SEE
Indirect
Assessme
nt
Methods
Standard
examination
End of course
survey
Students
End of course
100
Answer scripts
1,2,3&4
Questionnaire
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various educational
components
SL
Semester-End
Blooms Category
Test 1
Test 2
Test 3
NO.
Exam
1
Remember
20
20
20
30
2
Understand
30
30
30
25
3
Apply
25
25
30
25
4
Analyze
20
20
20
15
5
Evaluate
5
5
5
6
Create
0
0
0
0
Course Outcome: On completion of this course student will have improved ability:1. To apply spectroscopic techniques to characterize biomolecules.
2. To correlate structure-function relationships of macromolecules.
3. To apply laws of physics, chemistry and computer science to instrumental techniques of
analysis.
68
A
X
X
X
X
B
X
X
X
c
X
X
X
Program Outcome
D
e
f
g
h
X
X
X
X
X
X
i
X
X
X
X
X
X
Unit 4
Making of proteins and Health care: Native and fusion proteins, Yeast expression systems, The
baculovirus expression system, Mammalian cell lines. Creation of Hybrid proteins: StEP and ITGHY
method. Improving enzymes: Oxidation-resistant variants of a1-antitrypsin (AAT). Enhanced
Recovery and Folding of Recombinant Proteins Using Fusion Protein Strategies. Protein Engineering
for Affinity Purification: The Strep-tag Protein Engineering in Vaccine Development. Expression and
Use of Tagged Cell Surface Receptors for Drug Discovery: Estrogen Receptor as a Target for New
Drug Discovery.
Unit 5
Applications of Protein Biocatalysis and Enzyme Engineering : Random and rational approach of
protein engineering; Directed evolution and its applications in the field of biocatalysis; Various
approaches of creating variant enzyme molecules; Future of biocatalysis; Ideal Biocatalyst.
Stabilization of Industrial Enzymes by Protein Engineering Engineering -Glycoside Hydrolases.
Production and potential applications of recombinant gastric lipases in biotechnology. Enzymes as
therapeutic agents: Use of genetically engineered DNase I and alginate Lyase for treatment of Cystic
Fibrosis.
Indirect
Assessment
Methods
CIE
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
10
Assignment
reports
1,2,3&4
Surprise Test
Once
10
Quiz
answers
1,2,3&4
Standard
examination
End of course
(Answering 5
of 10
questions)
100
Answer
scripts
1,2,3&4
Students feedback
Middle of the
course
Feedback
forms
End of course
Questionnaire
SEE
Students
End of course
survey
1,2,3&4,
delivery of the
course
1,2,3&4,
assessment
methods
Questions for CIE and SEE will be designed to evaluate the various educational
components
SL NO.
Blooms Category
Semester-End Exam
1
Remember
25
2
Understand
30
3
Apply
20
70
4
5
6
Analyze
Evaluate
Create
20
5
0
Course Outcome:
On completion of this course student will have improved ability to:
1. Analyze the fundamentals characteristics of protein that can be engineered.
2. Identify the different approaches to construct and design the proteins.
3. Appraise the manipulations of protein properties and its function.
4. Apply the scope and benefits of protein engineering in drug targeting, therapeutics and in
biocatalysts for industry.
Course Outcomes
1
2
3
4
A
X
X
X
X
B
X
X
X
X
D
X
X
X
X
E
X
X
X
X
F
X
X
H
X
X
X
X
J
X
X
X
X
K
X
X
X
X
Text Books:
1. Edited by T.E. Creighton, Protein structure: A practical approach, 2nd Edition, Oxford University
Press, 1997.
2. Edited by T.E. Creighton, Protein Function: A practical approach, 2nd Edition, Oxford University
Press, 1997.
3. Cleland and Craik,Protein Engineering, Principles and Practice, Vol 7, Springer Netherlands 1998.
References:
1. Mueller and Arndt, Protein Engineering protocols, Ist Edition, Humana Press, 2006
2. Ed. Robertson DE, Noel JP, protein Engineering Methods in Enzymology, 388 Elsevier Academic
Press 2004.
3. J Kyte, Structure in protein chemistry, 2nd Edition, Garland publishers, 2006.
4. Protein engineering in industrial Biotechnology Edited by Lilia Alberghina
5. Commercial Biosensors Graham Ramsay, John Wiley Publishers.
Bioreactor Technology
Sub Code: MBTE13
CIE: 50 Marks
Credits: 4:0:0
SIE: 50 Marks
Text Books:
4. Tapobrata Panda., Bioreactors: Analysis and Design, 1st Edition, Tata McGraw Hill Education
Private Limited, New Delhi, 2011.
5. Mitchell, David A., Krieger, Nadia, Berovic, Marin. Solid-State Fermentation Bioreactors:
Fundamentals of Design and Operation, Springer-Verlag Berlin Heidelberg, 2006.
6. Michael C. Flickinger. Upstream Industrial Biotechnology. John Wiley & Sons, Inc, 2013.
7. Hee-Deung Park, In-Soung Chang, Kwang-Jin Lee. Principles of Membrane Bioreactors for
Wastewater Treatment. CRC Press, 2015.
Reference Books:
1. Kee-Yoeup Paek, Hosakatte Niranjana Murthy Jian-Jiang Zhong. Production of Biomass and
Bioactive Compounds Using Bioreactor Technology., Springer Science+Business Media
Dordrecht, 2014.
2. William L. Hochfeld. Producing Biomolecular Substances with Fermenters, Bioreactors, and
Biomolecular Synthesizers. CRC Press. 2006.
3. Sar faraz K. Niazi., Disposable Bioprocessing Systems, CRC Press., 2012.
4. Regine Eibl, Dieter Eibl, Ralf Prtner, Gerardo Catapano, Peter Czermak., Cell and Tissue
Reaction Engineering., Springer-Verlag Berlin Heidelberg, 2009.
Course Delivery: Regular black board teaching and interaction through tutorial classes.
Indirect
Assessment
Methods
CIE
Internal
assessment tests
Assignment
Surprise Test/
Quiz/ Mini
Project
SEE
Max
marks
Evidence
collected
Contributing
to Course
Outcomes
30
Blue books
1,2,3&4
Once
10
Once
10
End of course
(Answering 5 of
10 questions)
100
Answer
scripts
1,2,3&4
End of course
Feedback
forms
1,2,3&4 &
Delivery of
the course
Students
Standard
examination
Students feedback
When/ Where
(Frequency in
the course)
Thrice(Average
of the best two
will be
computed)
Students
Assignment/
reports
Blue
books/Quiz
answers
1,2,3&4
1,2,3&4
Questions for CIE and SEE will be designed to evaluate the various educational components:
S.No
Blooms Category
Test 1
Test 2
Test 3
SEE
1
Remember
20
10
10
10
2
Understand
25
20
20
20
3
Apply
25
20
20
25
4
Analyze
30
25
20
25
5
Evaluate
0
15
20
20
6
Create
0
0
0
0
73
Course outcome:
1. To analyze the principles of design, operation and major components of industrial bioreactors.
2. To explain the various aspects of Submerged Liquid Fermentation and Solid State fermentation
bioreactors.
3. Have knowledge of bioreactors designed for cell culture technologies in biopharmaceutical
industries.
4. Elucidate the underlying principles of Membrane bioreactors and apply them for related
industrial applications
74