Professional Documents
Culture Documents
Monitoring of
I m m u n o s u p p re s s i v e
Therapy Used in
Systemic Autoimmune
Diseases
Keith C. Meyer, MD, MS, FCCPa,*,
Catherine Decker, PharmD, AE-Cb,
Robert Baughman, MD, FCCPc
KEYWORDS
Immunosuppressive drugs Adverse drug reactions
Connective tissue disease Autoimmunity
a
Section of Allergy, Pulmonary and Critical Care Medicine, Department of Medicine, University of Wisconsin
School of Medicine and Public Health, 600 Highland Avenue, Madison, WI 53792, USA
b
Department of Pharmacy, University of Wisconsin Hospital and Clinics, University of Wisconsin School of
Pharmacy, Adult Pulmonary Clinic, 600 Highland Avenue, Madison, WI 53792, USA
c
Interstitial Lung Disease and Sarcoidosis Clinic, Department of Medicine, University of Cincinnati Medical
Center, Eden Avenue & Albert Sabin Way, Cincinnati, OH 45267, USA
* Corresponding author.
E-mail address: kcm@medicine.wisc.edu
chestmed.theclinics.com
In addition to the extrapulmonary disease manifestations and nonpulmonary organ system dysfunction patterns that characterize specific forms of
connective tissue disease (CTD), systemic autoimmune disorders frequently involve the lung and
can lead to pulmonary fibrosis and/or pulmonary
hypertension or vasculitic hemorrhage. Corticosteroids continue to be a mainstay of therapy,
especially for acute flares of lung disease.
However, other agents may be more effective in
achieving remission and stability of the disease
and allow corticosteroid dosages to be reduced
to low levels, particularly in chronic phases of the
disease (Table 1).
Corticosteroids and nonsteroidal antiinflammatory drugs (NSAIDs), once primary therapies for
many of the systemic autoimmune diseases
such as rheumatoid arthritis (RA), were eventually
supplanted in the 1980s and 1990s by antimetabolite/cytotoxic agents. These disease-modifying
566
Table 1
Summary of mechanism of action, routes of administration, and metabolism
Route of Administration
Drug Class
Specific Drug
Mechanism of Action
IV
Oral
Corticosteroid
Prednisone or
methylprednisolone
Anti-TNF
Agents
Adalimumab
MTX
MPA derivatives
Etanercept
Infliximab
Antimetabolite/
Cytotoxic
Agents
AZA
Cyclophosphamide
Leflunomide
Other
Chloroquines
Imatinib mesylate
Rituximab
Other
SC
SC
1
1
Abbreviations: IM, intramuscular; NA, nucleic acid; RES, reticuloendothelial system; SC, subcutaneous; TNF, tumor necrosis factor.
SC
IM
Table 2
Adverse drug reactions (ADR) associated with immunosuppressant agent administration
Specific Drug
Corticosteroids
Prednisone
Methylprednisolone
Anti-TNF
agents
Adalimumab
Etanercept
Infliximab
Antimetabolite/ AZA
Cyclophosphamide
cytotoxic
Leflunomide
agents
MTX
MPA derivatives
Other agents
1
1
1
1
1
1
1
1
?
?
NA
?
1
1
1
1
1
1
11
1
1
1
1
1
1
Chloroquine/
hydroxychloroquine
Imatinib mesylate
NA
Rituximab
1
1
?
1
?
?
?
11
1
11
1
?
11
1
1
1
1
1
11
1
1
11
1
1
1
1
1
11
1
1
11
?
1
11
1
11
11
?
?
1
1
1
1
1
1
1
1
?
?
1
1
1
1
1
1
11
?
1
1
1
1
1
1
1
Abbreviations: Bblank, no medication-associated ADR reported; NA, not applicable; , ADR suspected to be associated with medication use, yet is infrequent; 1, ADR associated with
medication use exists ; 11, ADR associated with medication use exists and is noted in greater frequency than 1; ?, unknown association of ADR with medication use.
Drug Class
Injection
Skin
CardioRenal/
BoneSite/
Pulmonary Ocular Reactions/
marrow
Neurologic GI
Hepatic Genitourinary vascular Dyslipi- MaliInfusion
Effects
demia
gnancy Toxicity
Toxicity Rash
Effects Effects Effects
Reactions Infection Suppression Effects
567
568
Meyer et al
biologic (immunomodulatory/antibody-based)
agents.
SPECIFIC AGENTS
Corticosteroids
Systemic corticosteroids are valuable in treating
acute disease but may have serious side effects.2
A myriad of complications (Box 1) may and often
do occur when high doses and/or prolonged
therapy with corticosteroids are prescribed.
Some of these complications can be controlled
and even prevented, such as fluid retention and
weight gain. Steroid-sparing agents may allow
control of disease processes and thereby minimize systemic corticosteroid administration and
associated adverse effects. Patients should be
educated about potential side effects and
informed of strategies that may limit some of the
adverse effects of corticosteroids. In addition,
patients should be monitored for the onset of
systemic hypertension, excessive weight gain/
fluid retention, gastrointestinal (GI) symptoms,
increased blood glucose, osteoporosis, hip pain,
and dyslipidemia (Table 3). A diet and regular
aerobic exercise that diminishes risk for heart
disease should be followed along with periodic
monitoring of a peripheral blood lipid profile. A
bone mineral density scan should be obtained at
baseline and at subsequent periodic intervals,
Box 1
Complications of corticosteroid therapy
Osteopenia/osteoporosis
Systemic hypertension
Weight gain
Glucose intolerance/diabetes
Dyslipidemia
Accelerated atherosclerosis
Myopathy
Infection
Avascular necrosis of bone
Cataracts
Glaucoma
Atrophy of skin
Adverse psychological effects
Disrupted sleep
Growth retardation
Cushingoid changes
Dyspepsia
BIOLOGIC AGENTS
Antitumor Necrosis Factor Agents
Biologic agents directed against tumor necrosis
factor a (TNF-a) have proved successful in treating
various manifestations of RA. These include the
TNF receptor antagonist etanercept3 as well as
the chimeric, humanized monoclonal anti-TNF
antibody infliximab4 and the humanized monoclonal antibodies adalimumab5 and golimumab.6
Etanercept is a soluble, fully human recombinant
form of the p75 tumor necrosis factor receptor
fused with the Fc portion of IgG1. In contrast to
the anti-TNF antibodies, etanercept acts by
binding to endogenous-soluble and membranebound TNF, thereby preventing its interaction
with TNF receptors and rendering it biologically
inactive.7 In addition to treatment of RA, anti-TNF
antibodies also have regulatory approval for the
treatment of psoriasis, ankylosing spondylitis,
ulcerative colitis, and Crohn disease.8
In the past 10 years, anti-TNF agents have been
given to more than a million patients, and several
guidelines for monitoring their use have been
proposed.911 Potential toxicities linked to the
administration of these drugs include infection,
infusion or injection reactions, worsened congestive heart failure (CHF), demyelination syndromes,
lupus-like reactions, and increased risk of malignancy.1216
Infliximab
An estimated 2-fold risk of infection is the most
common adverse event associated with infliximab
use.17,18 Most upper respiratory tract or urinary
tract infections have not been serious, but casecontrolled studies have suggested an increased
incidence of opportunistic infections.17 Although
no prospective, randomized data exist, some
reports have suggested that the risk of developing
tuberculosis may be greater with infliximab than
with other anti-TNF agents,1921 and tuberculosis
infections are also more likely to be
extrapulmonary.19,21,22
Existent data are not sufficient to determine if
lymphoma or nonlymphomatous malignancies
are increased.17 Hepatosplenic T-cell lymphomas
have been reported in rare postmarketing reports,
particularly in adolescent23 and young adult
patients with Crohn disease,24 and have exclusively occurred in patients concomitantly treated
with azathioprine (AZA) or 6-mercaptopurine.
Table 3
Summary of monitoring precautions
Drug Class
Specific Drug
Corticosteroids
Methylprednisolone
Prednisone
Anti-TNF agents
Adalimumab
Etanercept
Infliximab
Antimetabolite/
cytotoxic agents
AZA
Cyclophosphamide
Leflunomide
MTX
MPA derivatives
Other agents
Chloroquine/
hydroxychloroquine
Imatinib mesylate
Rituximab
569
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Meyer et al
generally resolved within 6 weeks to 14 months of
discontinuation.25 In addition, hematological side
effects such as leukopenia, neutropenia, thrombocytopenia, and pancytopenia have been occasionally reported.17 Severe hepatic reactions have also
been reported, and infliximab has been associated
with reactivation of hepatitis B virus infection.24,30
Adalimumab
As with infliximab, adalimumab administration has
been associated with increased infection risk,
especially tuberculosis,31 and serious infections
have been associated with the use of adalimumab.32,33 However, the rate of serious infection
was not statistically different from that encountered in the comparator groups in 2 different
trials.34,35 Adalimumab has been associated with
a cluster of cases of Legionella pneumophila pneumonia,36 and Pneumocystis jiroveci pneumonia
occurred in a patient receiving adalimumab.37
Autoimmune disease seems to be induced by
anti-TNF agents.13 In a detailed analysis of a large
cohort of cases, 15 of 92 cases of a lupuslike reaction were treated with adalimumab13 and vasculitis
was in reported in 113 patients, 5 of whom were
treated with adalimumab. Nervous system events,
including demyelination disorders, have been
sporadically reported in patients treated with
adalimumab.17,38
Etanercept
Serious injection site reactions can occur, and
injection site-associated necrotizing fasciitis has
been reported.39 Reactivation of latent tuberculosis has been associated with TNF inhibitors,40
but it is not clear whether etanercept therapy
increases the risk more than the already increased
risk of reactivation tuberculosis for patients with
RA.41 In the available case series, infection with
tuberculosis was extrapulmonary in half the cases,
suggesting that etanercept does have a role in
inhibiting control of this organism.41 However,
the soluble TNF fusion receptors, which include
etanercept, seem to be associated with less risk
of reactivation TB than the monoclonal TNF antagonists, infliximab and adalimumab.41 It is not clear
whether etanercept has adverse effects if given to
patients with CHF, but there have been case
reports of de novo cardiac failure or worsening of
preexisting cardiac dysfunction in patients treated
with etanercept.42 Sarcoidosislike reactions have
been reported, and seem to occur more frequently
with etanercept than with other anti-TNF agents.43
Non-Hodgkin lymphomas have been reported in
patients treated with etanercept, including several
in whom the lymphoma regressed following
cessation of the drug.44,45 However, the baseline
ANTIMETABOLITE/CYTOTOXIC AGENTS
AZA
AZA is a purine analog that is cleaved in vivo to its
active antimetabolite, 6-mercaptopurine. Its mechanisms of action remain unclear, but it suppresses
delayed hypersensitivity responses and cellmediated cytotoxicity via its effects on purine
metabolism, which likely inhibit DNA and RNA
synthesis, and, consequently, protein synthesis.63
Recent investigations suggest that AZA and its
metabolites induce T-cell apoptosis by binding to
the guanidine triphosphatase, Rac1.64 AZA and
mercaptopurine are degraded in erythrocytes
and the liver via oxidation or methylation.
Mercaptopurine undergoes thiol methylation
intracellularly via thiopurine methyltransferase
(TPMT), which converts it to thiopurine analogs.
Deficiency of this enzyme occurs in approximately
1 in 300 individuals and may lead to severe myelosuppression.65 Intermediate concentrations of
TPMT have been linked to severe side effects
related to AZA, and measuring TPMT activity
Cyclophosphamide
Cyclophosphamide (CYC), a synthetic alkylating
agent, is chemically related to the nitrogen
mustards and was originally developed for treatment of malignant tumors. It has antineoplastic
and immunosuppressive properties and has
received regulatory approval for the treatment of
various hematologic and nonhematologic malignancies. It has a dose-dependent, bimodal effect
on the immune system. High doses have been
shown to induce an antiinflammatory immune
deviation (ie, suppression of TH1 and enhancement of TH2 lymphocyte activity), affect
CD4CD25(high) regulatory T cells, and establish
a state of marked immunosuppression.79 It has
been used to treat various CTD-associated lung
571
572
Meyer et al
diseases such as scleroderma80,81 and other
forms of interstitial lung disease (ILD).82,83 It has
also been used as a standard treatment of
ANCA-associated vasculitis.84
CYC is metabolized in the kidney and liver. The
liver converts CYC to the active metabolites aldophosphamide and phosphoramide mustard, which
cause cell death by binding DNA and thereby
inhibiting DNA replication. CYC and its metabolites
are excreted via the kidney. CYC can be administered via either continuous daily dosage (oral) or
intermittent intravenous (IV) pulse therapy. For
several conditions, which include ANCAassociated vasculitis84,85 and sclerodermaassociated lung disease,80,81 the pulse therapy
seems to be as effective as and less toxic than
daily oral regimens. One of the disadvantages of
IV pulse therapy is the associated cost and need
for support staff to administer IV CYC. One of the
advantages of pulse therapy is that individual
doses of the drug can be adjusted based on the
patients individual white blood cell (WBC) count.
In one study, dosage adjustments were made in
almost one-third of visits.83 Regardless of the
method of administration (IV vs oral), the same
guidelines for monitoring apply.
Hemorrhagic cystitis and fibrosis of the urinary
bladder can occur, and hematuria frequently
occurs. Although hemorrhagic cystitis is an infrequent complication, it can be severe and life
threatening. Bladder toxicity likely occurs from
the CYC parent compound and several CYC
metabolites including acrolein. Measures that are
recommended to limit hemorrhagic cystitis include
ingesting more than 3 L of water or other fluids
daily. Sodium 2-sulfanylethanesulfonate (MESNA)
binds acrolein and some other CYC metabolites
in the urinary bladder and can attenuate toxicity.81
A large, prospective study of patients with Wegener granulomatosis reported that hematuria
occurred in a time- and dose-dependent
manner.86 Of 145 patients studied, 73 (50%)
developed nonglomerular hematuria, 7 of whom
had bladder cancer. Hematuria occurred a median
of 37 months after initiating CYC. Overall, 15% of
the patients developed hematuria within the first
year of treatment. In the placebo-controlled trial
of oral CYC versus placebo for sclerodermaassociated lung disease, 9 of the CYC-treated
patients developed hematuria versus 3 of the
placebo group.80
Bladder cancer is increased in individuals who
have received CYC, and cigarette smoking further
increases risk. Bladder cancer is also increased in
frequency in CYC-treated patients who have had
hemorrhagic cystitis.86 Bladder cancer seems to
be dose and duration dependent, with at least
MTX
MTX is approved in the United States for treatment
of RA, psoriasis, and several malignancies. It has
also been used to treat pulmonary sarcoidosis,99,100 and for induction and maintenance
therapy for Wegener granulomatosis 101,102 and
for
ILD
associated
with
polymyositisdermatomyositis.103
MTX is converted to polyglutamates in the liver,
and polyglutamate-mediated inhibition of extracellular adenosine metabolism is believed to account
for its immunosuppressive effects.104,105 The
plasma half-life of MTX is only 8 to 15 hours, but
its immunosuppressive actions can be measured
573
574
Meyer et al
636 total patients with RA or psoriasis from 15 published studies concluded that 28% of patients progressed at least one grade while on therapy.117 Five
percent of the patients had advanced liver disease
(grade IIIB or IV), and the major risk factors for
developing liver damage included a large cumulative dose of MTX, heavy alcohol use, and underlying psoriasis. Patients had a 6.7% chance of
progressive liver damage for each cumulative
gram of MTX. These data led to the recommendation that liver biopsy should be considered after
each 1 to 1.5 g of cumulative MTX.116 However,
monitoring liver function tests on a regular basis
for patients with RA has been found to be sufficient
to screen for potential MTX-associated liver
toxicity,118 which may be because of a lower incidence rate of severe liver toxicity in patients with
RA versus patients with psoriatic arthritis.119
Duration of exposure to MTX likely accounts for
a substantial proportion of its toxicity. Therefore,
once-weekly regimens are favored, and lead to
fewer side effects. Delayed clearance of the
medication (because of renal insufficiency, the
presence of third-space effusions, or GI obstruction) leads to prolonged circulating MTX levels
and increases the risk of toxicity.120122 Risk for
many of the bothersome side effects can be minimized by using folic acid. Typical doses are 1 to 2
mg/d, although daily doses up to 5 mg have been
described. Because MTX is a dihydrofolate
reductase inhibitor, folic acid supplementation
may bypass the MTX-induced blockade of nucleic acid synthesis. A well-designed randomized
trial of folic acid supplementation versus placebo
in RA patients showed that MTX at 5 mg or 27.5
mg/wk with folate supplementation had no effect
on drug efficacy in patients with RA, but it significantly reduced toxicity scores for both doses.123
A second, larger study (n 5 434) in patients with
RA reported similar results; MTX-related toxicity
led to discontinuation in 38% of placebo-treated
subjects versus 17% of those taking 1 to 2 mg
of folic acid daily and had no effect on
efficacy.124
Patients on MTX should undergo routine CBCs
and renal function,125,126 and the dose of MTX
should be adjusted if leukopenia is detected. In
one study, 26% of patients had one or more hematologic abnormality. More than 95% of these
cases had symptoms consistent with viral infection, and the observed abnormality resolved within
a month of withholding MTX and did not recur with
rechallenge.127 Liver function, especially the transaminases, should also be monitored, with routine
liver function testing usually performed every 4 to
12 weeks while the patient is taking the
drug.118,126,128 Liver biopsies to monitor for
Leflunomide
Several large clinical trials led to regulatory
approval of leflunomide for treatment of RA,130133
and clinical trials have been reported for psoriasis
and psoriatic arthritis, Sjogren syndrome,
systemic lupus erythematosis, ankylosing spondylitis, and Wegener granulomatosis.130,132,134138 Its
use has also been described for antisynthetase
syndrome, relapsing polychondritis, adult-onset
Still disease, and scleroderma.139142 It antagonizes T- and B-lymphocyte activation and proliferation by inhibiting de novo pyrimidine synthesis.143
Leflunomide is metabolized in the liver and bowel
wall to an active metabolite that is primarily eliminated via the biliary tract, although some renal
excretion also occurs.144 It undergoes extensive
enterohepatic recirculation, with an elimination
half-life of approximately 2 weeks.
The most common side effects reported in
controlled trials include nausea, diarrhea,
alopecia, hypertension, increased liver enzymes,
and rash. These side effects seem to be dose
related, and often resolve with dose reduction.
Postmarketing experience has shown rare
instances of serious infection, cytopenias, angioedema, fulminant hepatitis, interstitial pneumonitis,
peripheral neuropathy, and severe dermatologic
syndromes
(Stevens-Johnson
syndrome,
erythema multiforme, toxic epidermal necrolysis).
However, postmarketing data are severely limited
by ascertainment bias, concomitant immunosuppressive use, and the presence of the underlying
disease; therefore, attribution of these toxicities
to leflunomide remains unclear. Although leflunomide increases renal excretion of uric acid and
decreases tubular reabsorption of phosphate,145
clinically significant hypophosphatemia was not
identified in a large clinical trial.131
Hepatic toxicity generally occurs within the first
6 months of therapy, and it is more common in
Mycophenolate
Mycophenolic acid (MPA) selectively and potently
inhibits T- and B-lymphocyte proliferation by inhibiting the de novo purine pathway,154 and it has
regulatory approval for the prophylaxis of organ
rejection in cardiac, liver, and renal transplantation
in combination with cyclosporine and corticosteroids. MPA has been used to treat lupus
nephritis,155 RA,156 Wegener granulomatosis,157
systemic lupus erythematosus,158 and pulmonary
disease associated with various collagen vascular
disorders.159
Two forms of mycophenolate are currently available for prescription.160 Mycophenolate mofetil
(MMF), which is administered orally, is the morpholinoethyl ester prodrug of MPA, and it is rapidly
hydrolyzed (within 5 minutes) in the GI tract to
MPA. Mycophenolate sodium is a delayedrelease enteric formulation of MPA. MPA may
accumulate in end-state renal failure, requiring
hemodialysis or peritoneal dialysis, and dose
attenuation may be required. MPA is extensively
conjugated to glucuronide, which is predominantly
cleared via renal excretion, and MPA pharmacokinetics do not seem to be altered in patients with
hepatic dysfunction.161 Cardiovascular (systemic
hypertension, peripheral edema, tachycardia),
dermatologic (rash, neoplasm), endocrinologic
(hyperglycemia, cushingoid change, hirsutism),
metabolic (hypercholesterolemia, hypophosphatemia, hypokalemia, hyperkalemia), GI (nausea,
anorexia, vomiting, abdominal pain, diarrhea, constipation), hematologic (anemia, red blood cell
aplasia, leukopenia, thrombocytopenia, leukocytosis), infectious (opportunistic infection), musculoskeletal (bone pain, myalgias, cramps),
neurologic (headache, tremor, insomnia, dizziness, anxiety), ocular (blurred vision, cataracts,
blepharitis, keratitis, glaucoma, macular abnormalities), genitourinary (infection, hematuria,
tubular necrosis, urinary frequency, burning on
urination, kidney stones, vaginal burning, vaginal
bleeding), and respiratory (cough, dyspnea, infection, pneumonitis, fibrosis) abnormalities have all
been associated with MPA administration. Mycophenolate usually lacks a significant effect on
hematopoiesis and neutrophil populations
because other cell types (nonlymphocytic) can
use salvage pathways to obtain guanine.
However, neutropenia can occur and may respond
to simple modification of mycophenolate
dosage.162
Several drug interactions can occur with mycophenolate.163 Activated charcoal, aluminum or
magnesium salts, cholestyramine, colesevalam,
colestipol, or iron can inhibit absorption from the
GI tract.164 Mycophenolate should not be coadministered with AZA because of increased inhibition of purine metabolism, and mycophenolate
can increase plasma concentration of acyclovir
or ganciclovir, especially when renal impairment
is present. Mycophenolate may decrease exposure to hormonal therapies, and live attenuated
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Meyer et al
virus vaccines should not be given concomitantly
with MPA. Patients receiving MPA may have an
inadequate
immunologic
response
to
vaccination.165
CBCs have been recommended on a weekly
basis for the first month, twice monthly for the
second and third months of treatment, and then
once a month for the remainder of the first year
of treatment. However, when mycophenolate is
not given together with other agents that can
synergistically depress bone-marrow function,
such as calcineurin inhibitors, obtaining CBCs on
such a frequent schedule is probably not necessary. Plasma levels of MPA can be used to guide
therapy as well as to detect toxicity.166,167 In addition, MPA serum concentrations may be useful in
patients with impaired renal function to prevent
toxicity caused by high levels and graft rejection
caused by low levels.168 However, it has not
been conclusively shown that monitoring levels
minimizes toxicity or risk for rejection.169,170
OTHER AGENTS
Chloroquine/Hydroxychloroquine
Imatinib mesylate, a protein-tyrosine kinase inhibitor, inhibits cellular proliferation and induces
apoptosis in a variety of abnormal cell lines. It
has received regulatory approval for the treatment
of chronic myeloid leukemia and GI stromal tumor.
In addition to suppressing the specific kinase
dysfunction created by the gene mutations for
those malignancies, imatinib inhibits receptor tyrosine kinases for platelet-derived growth factor
(PDGF) and stem cell factor (SCF), and it inhibits
PDGF- and SCF-mediated cellular events. Imatinib has been used in fibrotic lung diseases and
pulmonary arterial hypertension.182184
Imatinib has high oral bioavailability (98%), and
the parent and active N-desmethyl derivative are
cleared predominantly via hepatic CYP3A4
enzymes.
Bullous
dermatologic
reactions
(including erythema multiforme and StevensJohnson syndrome), significant fluid retention, GI
complications, and hematologic abnormalities
have been reported.185187 Fluid retention (which
can manifest as pleural effusions, ascites, pulmonary edema, or peripheral edema) occurs more
frequently in patients older than 65 years. Neutropenia, anemia, and thrombocytopenia have been
linked to imatinib therapy, and the package insert
recommends a CBC weekly for the first month,
biweekly for the second month, and periodically
thereafter as clinically indicated.
Hepatic impairment, which can be severe, has
been reported. Liver function testing (transaminases, bilirubin, and alkaline phosphatase) is recommended at baseline and then monthly and/or
as clinically indicated per the package insert.
Available data are insufficient to determine if
Imatinib Mesylate
DRUG-DRUG INTERACTIONS
Numerous drug-drug interactions may occur,
especially with use of the nonbiologic agents.
These interactions are summarized in Table 4.
Drug-drug interactions must be considered when
new drugs are administered as well as when coadministered drugs are withdrawn or doses are
altered, which is particularly important for drugs
that are metabolized by the cytochrome
P-450CYP3A4 enzyme system. Drug levels of
CYP3A4-metabolized agents (eg, imatinib) can
be significantly increased by administration of
drugs that are also metabolized via this pathway
or reduced by coadministration of CYP3A4
inducers. Appropriate dose adjustment and monitoring should be observed if a CYP3A4 drug is
administered and inhibitors or inducers of this
enzyme are coadministered. Many commonly
prescribed agents (eg, imidazole antifungal
agents, macrolide antibiotics) can significantly
affect blood levels of the CYP3A4-metabolized imatinib mesylate. In addition, most of the agents
covered in this article can suppress antibody
responses to vaccines. In addition, recipients
may be endangered if live virus vaccines are given
while the patients are receiving pharmacologic
immunosuppression. Potentially significant interactions have been documented for some of these
agents with ingested substances such as grapefruit juice, echinacea, and herbal preparations or
supplements. Patients should be aware of these
potential interactions.
PREGNANCY
All of the nonbiologic immunosuppressive agents
may have teratogenic or embryocidal effects on
the fetus and have been placed in the US Food
and Drug Administration (FDA) categories of C,
D, or X; none has been assigned to category A
(Table 5).190 CYC is teratogenic during pregnancy
(FDA category D), and it should not be given to
women with childbearing potential without
adequate measures to prevent pregnancy.
However, CYC has been administered successfully during the third trimester when considered
PEDIATRIC DOSING
There are limited data on the use of immunosuppressive drugs for the treatment of inflammatory
disorders in children. Antimalarial agents have
been used to treat rheumatologic diseases in children for many years, and these drugs are generally
felt to be safe despite limited published literature.
Toxicities for older children are generally believed
to parallel those for adults. However, this may
not be the case for certain situations. Pharmacokinetic parameters and mycophenolate levels were
found to be similar for adults and children in one
study, but mycophenolate levels were disproportionately increased in children when kidney function was impaired and/or albumin levels were
depressed.193 Pharmacokinetic monitoring in children receiving MTX revealed that the maximum
dose appeared to be 10 mg/m2 for children and
differed from dosing for adult patients.194 In addition, newer approaches to treating children with
immunosuppressive therapies are evolving in
which certain agents, such as corticosteroids,
are minimized.195,196 Monitoring strategies may
also be helpful in children to help avoid toxicity
from some drugs. Monitoring metabolite levels in
patients treated with AZA may help to minimize
toxicity and enhance treatment efficacy.197
577
578
Table 4
Summary of drug-drug interactions
Specific Drug
Imidazole
Metabolized Antifungal Psychiatric Antibacterial
by CYP3A4) Agents
Therapies Agents
GI Acid
Anakinra/
Reducers
MTX/Myco(Proton Pump
Steroidal Antiarrythmic Anticonphenolate/
ACE
Thiazide Inhibitor; H-2,
Therapies Therapies
vulsants Vaccines AZA
NSAIDs Inhibitors Diuretics Elements)
Allopurinol Coumadin
Adalimumab
Etanercept
Infliximab
AZA
Cyclophosphamide
Chloramphenicol
Leflunomide
Rifampin
Rifapentine
MTX
Penicillins,
Doxycyline
Mycophenolate
Norfloxacin
Chloroquines
Imatinib
mesylate
Rituximab
1
1
Quinolones
Trimethoprim
sulfa/Sulfa
macrolides
Pentamidine
Macrolides
Rifampin
Rifabutin
Rifapentine
1
1
1
1
Hydroxymethylglutaryl
Coenzyme
A Reductase
Herbal
Inhibitors
Supplements
CYP3A4-metabolized drugs (competitors; increased levels of other drugs metabolized by CYP3A4): nefazodone, macrolides, imidazoles, metronidazole, cisapride, cimetidine, chloramphenicol, grapefruit juice, calcium channel-blockers,
theophylline. CYP3A4 inducers (decreased drug level caused by increased activity of CYP3A4): phenytoin, phenobarbitol, modafinil, carbamazepine, quinopristine, rifampin, sulfasalazine, sulfinpyrazone.
Table 5
Effects on pregnancy and pregnancy classification
Pregnancy Category
Specific Drug
Prednisone
Methyprednisolone
Adalimumab
Etanercept
Infliximab
AZA
Cyclophosphamide
Leflunomide
MTX
Mycophenolate
Chloroquines
Imatinib
Rituximab
FDA
(United States)
Australia
None
B
B
B
D
D
X
X
D
C
D
C
C
B
None
D
D
None
D
D
D
D
C
A. Controlled studies in women fail to show a risk to the fetus in the first trimester (and there is no
evidence of a risk in later trimesters), and the possibility of fetal harm seems remote
B. Either animal-reproduction studies have not shown a fetal risk but there are no controlled studies in
pregnant women or animal-reproduction studies have shown adverse effect (other than a decrease in
fertility) that was not confirmed in controlled studies in women in the first trimester (and there is no
evidence of a risk in later trimesters)
C. Either studies in animals have revealed adverse effects on the fetus (teratogenic or embryocidal or
other) and there are no controlled studies in women, or studies in women and animals are not
available. Drugs should be given only if the potential benefit justifies the potential risk to the fetus
D. There is positive evidence of human fetal risk, but the benefits from use in pregnant women may be
acceptable despite the risk (eg, if the drug is needed in a life-threatening situation or for a serious
disease for which safer drugs cannot be used or are ineffective)
X. Studies in animals or human beings have demonstrated fetal abnormalities or there is evidence of
fetal risk based on human experience or both, and the risk of the use of the drug in pregnant women
clearly outweighs any possible benefit. The drug is contraindicated in women who are or may become
pregnant
579
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Meyer et al
aspergillosis, histoplasmosis, other endemic fungi,
pneumocystis pneumonia, cytomegalovirus pneumonia, viral hepatitis) during the course of treatment presents significant risks for patients
undergoing intensive pharmacologic immunosuppression. Infection may occur because of
suppression of immune cell function (eg,
T lymphocytes), bone-marrow toxicity with neutropenia, or a combination of depressed cell function
and decreased numbers of immune effector cells.
Pneumocystis pneumonia may complicate the
course of patients with systemic autoimmune
disease who are treated with intense immunosuppression.198200 If intensive immunosuppressive
therapy is prescribed, prophylaxis for P jiroveci
should be strongly considered.201,202 Although
screening for active or latent tuberculosis is supported only by the literature for anti-TNF therapy,
the administration of other potent immunosuppressive regimens could lead to reactivation of
latent infection. Ideally, patients should be
uniformly and carefully screened for evidence of
active or latent tuberculosis before the initiation
of any intense immunosuppressive therapies.
Many of the nonbiologic agents can significantly
depress bone-marrow function. Granulocytic cell
lines are most susceptible, and neutropenia may
complicate therapy by predisposing patients to
infection. Other hematopoietic cell lineages may
also be affected and lead to anemia or thrombocytopenia. Other drugs (eg, trimethoprim sulfa or
ganciclovir) given for prophylaxis or treatment of
infection may contribute to bone-marrow suppression and potentiate the effect of immunosuppressive drug therapies. Intermittent monitoring of
bone-marrow function via CBC with differential
cell count is advised for all patients receiving drugs
that are associated with potential bone-marrow
suppression.
GI toxicity including severe hepatotoxicity can
occur as a consequence of several nonbiologic
agents. MPA derivatives can significantly affect
intestinal transit times and often cause diarrhea,
which can be severe in some instances and not
necessarily correlate well with mycophenolate
blood levels. Although blood levels of mycophenolate are not routinely obtained to monitor this
agent, a level can be obtained if diarrhea complicates therapy, and a high level would implicate
mycophenolate as a potential cause of this
complication if other causes such as infection
are excluded. Serious hepatic injury can occur
with MTX, AZA, or leflunomide, and hepatic function should be intermittently monitored (eg, once
monthly) with appropriate testing while patients
are receiving agents that can potentially cause
serious hepatotoxicity.
SUMMARY
Immunosuppressive drug therapy for systemic
autoimmune disorders considerably increases
the risk of infection and various other complications that range from bone-marrow suppression
and hepatic dysfunction to pulmonary toxicity.
Provider knowledge of potential adverse reactions
to these drugs combined with the use of strategies
for pretherapy screening before drug administration plus appropriate interval clinical evaluation
and laboratory testing while on therapy are key
to avoiding severe complications of immunosuppressive drug therapy. In addition, patient education is important, and prophylactic measures to
prevent complications (eg, administration of folic
acid, trimethoprime-sulfa prophylaxis, appropriate
screening and treatment of osteopenia/osteoporosis) can benefit the patient.
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