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Bacterial Culture
APSC 302-02(3-5pm)/SPNG 2014
Name:
Chelsea Facison
Lab Partner: Taurus Thompson
Date performed: 2/12/15 - 2/19/15
Date Lab Submitted:2/26/15
Instructor: Dr. Min
Objective:
To demonstrate colony counting for determining different types of bacterial
growths and to observe the effect of age (time) on milk bacterial colony formation.
Procedure:
Day 1:
Materials:
50 grams of MacConkey Agar
950 milliliters of Distill water
Procedure:
1. Mix solution and heat
2. Sterilize at 15lb pressure (121C) for 15mins
3. Temper sterile media at 47C 50C 50C for 1hr
4. Pour 13mL to 15mL per plate
5. Store inverted in a refrigerator
Day 2:
Materials:
Tools
Milk sample
Agar plate
Procedure:
1. Sterilize tools
2. using tool dip into milk
3. spread sample as described
1
2
3
Analysis:
Figure 1. Plate #1
Figure 2. Plate #2