Project 1

Bacterial Culture
APSC 302-02(3-5pm)/SPNG 2014
Name:
Chelsea Facison
Lab Partner: Taurus Thompson
Date performed: 2/12/15 - 2/19/15
Date Lab Submitted:2/26/15
Instructor: Dr. Min

Objective:
To demonstrate colony counting for determining different types of bacterial
growths and to observe the effect of age (time) on milk bacterial colony formation.
Procedure:
Day 1:
Materials:
50 grams of MacConkey Agar
950 milliliters of Distill water
Procedure:
1. Mix solution and heat
2. Sterilize at 15lb pressure (121C) for 15mins
3. Temper sterile media at 47C 50C 50C for 1hr
4. Pour 13mL to 15mL per plate
5. Store inverted in a refrigerator
Day 2:
Materials:
Tools
Milk sample
Agar plate

Procedure:
1. Sterilize tools
2. using tool dip into milk
3. spread sample as described

4. turn dishes over in inbuater at 38C for 2 days

1
2
3

Analysis:

On plate one there is only bacteria

growth on the third streak. It is
Klebsiella pneumoniae and
Pasteurella multocida. One the
second plate on the first and second streak it is
Klebsiella pneumoniae on the thirds streak is
Pasteurella multocida.
Pasteurella multocida is a type of bacterial that
commonly infects the respiratory tract of calves
causing bovine respiratory disease. Pasteurella
multocida is one of the most common bacteria

Figure 1. Plate #1

Figure 2. Plate #2

isolated from calves suffering from shipping

fever pneumonia. Pasteurella multocida is usually a secondary bacterial invader, meaning that a
virus or some other disease first weakens the immune system thus allowing Pasteurella
multocida to invade. Pasteurella multocida is found throughout the environment and within the
upper respiratory tract of cattle, but it usually does not cause disease in otherwise healthy
animals. Pasteurella multocida is considered part of the normal bacterial flora of the upper
respiratory tract of most cattle. Pasteurella multocida can cause disease when it is inhaled into
the deeper portions of the respiratory tract and the animals normal defense system is impaired.
Normally, inhaled bacteria like Pasteurella multocida is killed and removed by the bodys
antibodies and macrophages. When a calfs immune function is impaired by stress or diseases
such as bovine rhinotracheitis virus (IBR), parainfluenza virus (PI-3), or bovine respiratory
syncytial virus (BRSV) the bacteria can invade and cause disease. This is when you can see
necrotizing, fibrinous pleuropneumonia. Pasteurella multocida is spread via direct contact, or by
ingestion of feed and water contaminated by nasal and oral discharges from infected cattle.
Transmission of these bacteria is especially efficient when calves are crowded (as in shipment) or
closely confined (as in a dairy calf nursery).
Klebsiella pneumoniae is a common cause of clinical mastitis in dairy cattle. Wood
products are considered to be the main source of Klebsiella pneumoniae on dairy farms.
Environmental hygiene and use of inorganic bedding materials such as sand are recommended to
control Klebsiella pneumoniae mastitis. However, Klebsiella pneumoniae mastitis still occurs on
well-managed dairy farms that use sand as bedding material. Klebsiella pneumoniae can be
found in feed and water, manure and bedding was evaluated. Suggesting that animal hygiene
may be an important indicator of the risk of exposure to Klebsiella pneumoniae.