KX-21 START-UP

1. Pre-Operation Checks
Check Reagent Supply.

Refill Printer with paper.

Check the tubings and cables. Make sure they are not bent and the cord is securely plugged
into the powe outlet.

Check Pneumatic Unit trap chamber for fluid. Empty if needed.

Check waste container (if used). Empty if needed.

2. Power ON Sequence (switch is located on the right side of the Main Unit)
Printer (if K-DPS is connected)

K-DPS (if connected)

KX-21 Main Unit (Pneumatic Unit turns ON automatically)

3. Self Checks are performed (Please Wait would be displayed)

Microprocessor check

Motor

Background check

4. Acceptable Background Limits

WBC
RBC
HGB
PLT

< 0.3 ( x 103/uL)

< 0.02 ( x 106/uL)
< 0.1 (g/dL)
< 10
( x 103/uL)

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5. If Background Error occurs:

Press [HELP] key to stop alarm and to display the Action Message screen, then take appropriate
action.

KX-21 QUALITY CONTROL

Manual Mode Analysis
1. When the system is in READY status, press [SELECT] key.
2. Using up/down keys, move the cursor to select 2. Quality Control.
3. Press [ENTER] key. QC Chart screen appears.
4. Press [SAMPLE NO.] key and input the File #. To enter another File #, press [C] key to clear
and enter a new File #.
5. Press [ENTER] key, the QC Chart for the selected File appears.
6. Press [1] key to select 1. QC Analyze. The L-J Control Analysis screen appears.
7. Confirm that the instrument goes into READY status.
8. Mix the control blood thoroughly by inversion several times.
9. Remove cap and set the control by the aspiration pipette, then press [START] switch.
10. Remove the control blood after sound beeps twice.
11. Results are displayed. If data is acceptable, press [1] key to confirm and [2] to reject.
12. Press [3] key and select 3. Print to print out the results.

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Setting Target/Limit values

1. When the system is in READY status, press [SELECT] key.
2. Using up/down keys, move the cursor to select 2. Quality Control.
3. Press [ENTER] key. QC Chart screen appears.
4. Press [2] to select 2. Settings
5. Using up/down keys, move the cursor to select control parameters.
6. Using the left/right keys, move the cursor to select TARGET OR LIMIT.
7. Enter the values given on the assay sheet provided with Eightcheck using the numeric keys.
8. Press [ENTER], when settings are completed, press [SELECT] key.
9. Using left/right keys move the cursor to Set, then press [ENTER] to execute the selected
processes.

KX-21 SAMPLE PROCESSING

Whole Blood Mode (KX-21 sample volume 50 uL ).
Make sure the instrument is in the READY status.

Press [SAMPLE NO.] key to input sample ID # using the numeric keypad.

Press [ENTER] key, then the instrument turns into READY.

Mix the sample blood thoroughly by inversion several times.

Remove the cap carefully, then set the sample into the manual aspiration pipette.

Press the [START] switch.

Remove the blood sample after sound beeps twice.

Results are displayed on the LCD screen and can be automatically printed out.

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Pre-diluted Mode (200 uL of 1:26 dilution of sample blood)

Confirm that the instrument is in the READY status.

Press [MODE] key to display Change Mode screen.

Press left/right keys to select Pre-Diluted (PD), then press [ENTER].

Press [SAMPLE NO.] key and input the sample ID number using the numeric keys.

Press [ENTER] key, then the instrument turns into READY.

Set a well-mixed 1:26 diluted blood sample by the aspiration pipette.

Press the [START] switch.

Remove the blood sample after sound beeps twice.

Results are displayed on the LCD screen and can be automatically printed out.

After running all the Pre-diluted samples, change back to Manual Mode.

Preparing a 1:26 dilution of sample blood

Dilute 20 uL of sample blood with 500 uL of CELLPACK

KX-21 SHUTDOWN & MAINTENANCE

Daily Shutdown (or once every 24 hours)
SHUTDOWN KEY.
Press [SHUTDOWN] key and the Shutdown screen appears.
Set CELLCLEAN to the sample probe and press [START] switch.
Remove CELLCLEAN when the sound beeps twice. Process would take approx. 7 minutes.
Turn OFF the instrument when Turn Power OFF message appears.
CHECK PNEUMATIC TRAP CHAMBER FOR FLUID LEVEL.

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DOCUMENT ON THE MAINTENANCE CHECKLIST.

Weekly Maintenance
CLEAN SRV TRAY
Turn OFF the power. Wait at least 30 seconds to release the pressure and vacuum accumulated in the
pneumatic lines.
Open Front cover and set Stop Bar in position so that the cover would not close accidentally.
Remove the SRV tray by pulling it towards you.
Check the tray for dirt, wipe and rinse with water.
Reattach the tray in its original position.
Close the Front cover.
Document on the maintenance checklist.

Monthly Maintenance
CLEAN WASTE CHAMBERS
Press [SELECT] key in the Ready status. Select 7. Maintenance using the up/down keys.
Press [ENTER] key. Select 1. Clean Waste Chambers., then press [ENTER] key.
Place CELLCLEAN at sample probe, then press [START] switch to start process.
Remove CELLCLEAN when the sound beeps twice. Process would take approx. 15 minutes.
Document on the maintenance checklist.

CLEAN Transducer
Open the front cover and use the stop bar to prevent accidental closure.
Press [SELECT] key on the panel keypad.
Select 7. Maintenance using the up/down keys, then press [ENTER].
Select 2. Clean Transducer using the up/down keys.
Remove the WBC and/or RBC detector cap.
Pipet 1 mL of CELLCLEAN into each transducer chamber, replace the caps.
Close the transducer cover and close the front cover of the Main Unit.
Press the [START] switch.
Document on the maintenance checklist.

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Quarterly Maintenance
CLEAN SRV

Please refer to the operator manual for quarterly and As Needed Maintenance.

DEBASISH ROY
APPLICATION
TRANSASIA BIO-MEDICAL LTD.

Sysmex Singapore Pte Ltd