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Principles of TLC
Mobile phase and stationary phase
Principle steps in TLC separation
Application of TLC
Procedure:
(A) Preparation of TLC plate
1. Wear gloves when handling the TLC plates.
2. Cut the TLC plate into strips of 2 cm 10 cm in size.
3. With a pencil, draw a line approximately 1.5 cm from the short edge of the TLC plate.
Mark another line approximately 1.0 cm from the edge of the other side. Be careful to not
scratch the surface of the plate too deeply.
1.5cm
m
(B) Lipid detection
1 cm
Lab Manual
Color
Rf value
carotene
yellow-orange
0.93
pheophytin a
grey
0.55
pheophytin b
chlorophyll a
blue-green
0.46
chlorophyll b
green
0.42
xanthophylls
yellow
0.41
xanthophylls
yellow
0.31
xanthophylls
yellow
0.17
0.47-0.54