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The nervous system has a communication network of neurons that allows the organism to
interact with the environment both external and internal (Komal and Nashmi 2015). It is also
responsible for adaptation to changes such as maintenance of homeostasis and survival. It has
two subdivisions, the CNS and PNS. The CNS consists of the brain and the spinal cord, while
the PNS consists of motor portions (autonomic and somatic motor neurons) which transmits
information from periphery to the central nervous system and vice versa (Louveau et al., 2015).
For the activity, Bufo sp. was used. It was exposed to different environment where they received
different stimuli. The nerve responses of the animal in the normal condition were observed. After
the first testing, single pithing (ruptured brain) and double pithing (ruptured brain and spinal cord)
were performed consecutively. The animal was then again exposed to the same environments
used on the first procedure. The responses of Bufo sp. was also observed and recorded. The
sciatic nerve of the animal was also isolated and was observed under the microscope.
From the results obtained, it was observed that when single pithing was performed where the
brain is severed, the sensory responses of the animal was impaired. The frog was almost
unresponsive, except for the heart which only slowed in pulsation. On the second pithing
performed which is double pithing, the brain and the spinal cord where severed. The animal
completely loss its sensation and muscle control because the spinal cord was damaged. Its
muscle became firm which were supposed to be flaccid when brain and spinal cord was normal.
When these two were injured or destroyed, the whole system of the body shuts down.
Keywords: sensation, motor neurons, CNS, PNS, body response, stimuli
INTRODUCTION
METHODOLOGY
Single Pithing
After the first procedure was finished,
the animal undergone single pithing to
rupture or injure its brain. A probe was
inserted down on the midline of the head,
over a bump (as a reference point) which is
the occipital process until it came to the soft
spot of the foramen magnum. The probe was
moved from side to side to sever the brain.
The same procedure was repeated. It was
exposed to different environment. The
observations were recorded.
Double Pithing
After recording all the observations on
the animal with damaged brain, double
pithing was performed. The probe was
inserted downward towards the body of the
toad. The spinal cord and the brain of the
Bufo sp. was destroyed. The responses of
the animal to the same stimuli received from
being exposed to different environment were
recorded.
THICK-
5m X 4= 20 m
THIN-
4m x 4= 16 m
THICK-
9.7 m
THIN-
0.2 m
CONCLUSION
LITERATURES CITED
cord. Eur.
36483658
J.
Neurosci.,
11,
Komal, Pragya and Raad Nashmi (2015). Tcell receptors modify neuronal
function in the central nervous
system. Biochemical Pharmacology.
http://dx.doi.org/10.1016/j.bcp.2015.
07.023