The Final Exam Test A

BIBC 102, Metabolic Biochemistry

Wednesday, March 18, 2009

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This exam has

pages (including this cover page);
please check whether your exam is complete before you
start to answer questions.
The exam has 100 multiple-choice questions and each
question is worth 1 point
For all the questions, choose only one answer from the
choices offered, and enter it onto the appropriate space of
the scantron sheet. Use a number 2 pencil only
Youcankeeptheexambookletaftertheexam
You have 3 hours total to answer the questions.
The questions are NOT arranged in the order of difficulty.
The answer key will be posted on the course website
tonight.

Good luck!

Questions 1 to 15 are related to the following five compounds (I to V)

O
CH3-C
IV

II

O-

III

1) How many NET NADH are (is) required when the compound I is used as the starting
material to make a glucose molecule in animal cells?
A)
B)
C)
D)
E)

0
1
2
3
none of the above

2. How many NET GTP molecules are required when the compound I is used as a
starting material to make a glucose molecule?
A) 0
B) 1
C) 2
D) 3
E) 4
3. How many steps that use a phosphatase when the compound I is used as a starting
material to make a glucose molecule
A) 0
B) 1
C) 2
D) 3
E) 4

4. How many kinase(s) is (are) required when the compound III is used as a starting
material to make a glucose molecule?
A) 0
B) 1
C) 2
D) 3
E) 4
5. How many NET ATPs are needed to convert compound II to a glucose molecule?
A) 0
B) 1
C) 2
D) 3
E) 4
6. How many enzymes are needed to convert compound I to compound III
A) 1
B) 2
C) 3
D) 4
E) 5
7. How many enzymes are needed to convert compound V to compound III?
A) 0
B) 1
C) 2
D) 3
E) 4
8. Which of the compound CANNOT be converted to glucose in animal cells?
A) I
B) II
C) III
D) IV
E) V
9. Which of the compounds can be converted into amino acids in ONE step?
A) I and II
B) II and III
C) I and III
D) III and V
E) V and II

10. Which of the compounds are intermediates in TCA cycle

A) I and II
B) II and III
C) I and III
D) III
E) None of the above
11). Which of the compounds can be converted to fatty acids?
A) IV only
B) I, II, and III
C) IV and V
D) I, II, III, IV, V
E) III and IV
12) How many NET NADH are produced when one molecule of compound II is
completely oxidized to CO2.
A) 0
B) 1
C) 2
D) 3
E) 4
13) Oxidation of 1 molecule NADH produces 2.5ATP and oxidation of 1 molecule of
FADH2 produces 1.5 ATP. If the production of GTP counts as ATP, how many NET ATP
molecules are produced when one molecule V is completely oxidized to CO2 and water?
A) 13.5
B) 14.5
C) 15
D)15.5
E) 16
14) Conversion of 1 molecule V to 1 molecule II, How many kinases are needed?
A) 0
B) 1
C) 2
D) 3
E) 4
15 Which of the compound belongs to Ketone Bodies
A) I
B) III
C) IV
D) V
E) none of the above

16).WhichofthefollowingenzymeisNOTusedingluconeogenesis.
A)Pyruvatekinase
B)Triosephosphateisomerase
C)Dehydrogenase
D)Phosphoglucoseisomerase
E)Noneoftheabove
17)Whichofthefollowingpairofenzymecatalyzedreactionsmakesafutilecycle
A)PFK1andFructose2,6bisphosphatase
B)Hexokinaseandglucose1phosphatase
C)pyrovatekinaseandpyruvatecarboxylase
D)malicenzymeandmalatedehydrogease
E)noneoftheabove
18)Futilecycle
A)isalsocalledassubstratecycle
B)wasdiscoveredbyaNobellaureate
C)onlyhappensininsectssuchasbees
D)hasnoimportantphysiologicalroles
E)noneoftheabove
Questions20to22arerelatedtothefollowingfigure:

III

II
I

20) I is likely
A) NADH
B) ADP and Pi
C) oligomycin

D) DNP
E) CO
21) II is likely
A) NADH
B) ADP and Pi
C) oligomycin
D) DNP
E) CO
22) III is likely
A) NADH
B) ADP and Pi
C) oligomycin
D) DNP
E) CO
Questions 23 to 31 is related to the following diagram:

MoleculeA

Complex I

MoleculeX

Complex II

Complex III

MoleculeY
Complex IV

MoleculeZ

MoleculeB

23) Molecule A is likely

B) NADH
B) QH2
C) FADH2
D) succinate
E) can only do one electron chemistry
24) The complex II is
A) NADH-Q oxidoreductase
B) Succinate-Q reductase
C) Cytochrome c oxidase
D) Superoxide dismutase
E) None of the above
25) The complex IV is
A)
B)
C)
D)
E)

NADH-Q oxidoreductase
Succinate-Q reductase
Cytochrome c oxidase
Superoxide dismutase
None of the above

26) Molecule X is
A) Water soluble
B) A small protein
C) Can only do one electron chemistry
D) Linked to enzymes by a covalent bonds
E) None of the above
27) Molecule Y is
A) Water soluble
B) Contains a heme
C) Can only do one electron chemistry
D) Easily detectable using UV-visible spectroscopy
E) All of the above
28) Molecule Z
A) It takes 2 electrons to fully reduce molecule Z
B) It can only take 2 electrons each time
C) When molecule Z accepts electrons, it also uptake protons from the matrix
D) A and B
E) A and C

29) Which complex DOES NOT pump protons from the N side to the P side
A) Complex I
B) Complex II
C) Complex III
D) Complex IV
E) None of the above
30)MoleculeB
A)hasabsorbancemaximumat340nM
B)islikelysuccinate
C)iscoenzymeQ
D)containsheme
E)isNADHorsuccinate
31Qcycleoccursat
A) Complex I
B) Complex II
C) Complex III
D) Complex IV
E) None of the above
32) ATP synthase:
A)
B)
C)
D)
E)

accepts electrons during its catalytic cycle

does not have a proton channel
makes ATP by substrate level phosphorylation
is found in mitochondria but not in chloroplasts
can hydrolyze ATP in vitro

33)Thebindingchangemodel:
A) aprocessinvolvedinoxygenreduction
B) interconversionoftwoconformationsinATPsynthaseinducedbyprotonflow
C) proposedbyPeterMitchell
D) subunitrotationleadstointerconversionsbetweenconformationsofthesubunit
E) Noneoftheabove
Questions 34 to 36 are related to the following glycogen molecule
X

P
Y
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34)1,4linkageis
A) X
B) Y
C) BothXandY
D) EitherXorY
E) Noneoftheabove
35) Glycogen phosphorylase can cleave
A) Only X
B) Either X or Y
C) Y, but only after X is cleaved
D) X, but only after Y cleaved
E) None of the above
36) Non-reducing end refers to
A) P
B) Q
C) X
D) Y
E) None of the above
37) De-branching enzyme
A) Glucose is one of the products
B) Glucose-1-phosphate is one of the products
C) Glucose-6-phosphate is one of the products
D) UDP-Glucose is one of the products
E) A and B
38) Glycogen phosphorylase
A) usually functions as a tetramer
B) needs PLP as a cofactor
C) is never active when dephosphorylated
D) A and B
E) B and C
39) Protein kinase A
A) phosphorylates glycogen phosphorylase directly
B) phosphorylates glycogen synthase directly
C) is activated by insulin
D) A and B
E) A, B, and C

40)Liverglucogenphosphorylase
A)isallostericallyinhibitedbyATP
B)isallostericallyactivatedbyglucose
C) is activated by phosphorylation
D) A and C
E) B and C
41) Conversion of glucose-1-phosphate to glucose-6-phosphate
A) Catalyzed by a kinase
B) Involved in a phosphorylated protein intermediate
C) Is PLP dependent
D) Is UTP dependent
E) None of the above
42) The reaction catalyzed by glycogen phosphorylase
A) is SN2 type reaction
B) is SN1 type reaction with beta configuration at C1 in the product
C) Is SN1 type reaction with alpha configuration at C1 in the product
D) Involedinacarbanion
E) BandD
43) Dephosphorylated glycogen phosphorylase in muscle can be
A) activated by AMP
B) inhibited by ATP
C) inhibited by glucose-6-phosphate
D) activated by phosphorylation
E) all of the above
44) Photosynthesis
A) is also called light reactions
B) only occurs in plants
C) takes place at the inner membrane of chloroplasts
D) occasionally happens in mitochondria
E) none of the above
45) Chloroplast and mitochondria
A) Plants have chloroplasts, but do not have mitochondria
B) Plants uses chloroplasts for light reactions and mitochondria for dark reactions
C) ATP synthase is located on the inner membrane of both mitochondria and
chloroplasts
D) Chloroplasts and mitochondria are the same, but plant mitochondria are called
chloroplasts
E) None of the above
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46) Oxygen production in photosynthesis takes place at

A) Magnesium center
B) Manganese center
C) Heme center
D) Cupper center
E) None of the above
47) Which of the following is structurally most similar to chlorophyll
A) Fe-S cluster
B) Pheophytin
C) Coenzyme Q
D) FAD
E) TPP
48) Which statement regarding glycogen phosphorylase b is true?
A) ) phosphorylase b is never active
B) phosphorylase b is always monomeric
C) phosphorylase b is a homolog of phosphorylase a
D) phosphorylase b can be converted to glycogen synthase by phosphorylation
E) none of the above
49) Magnesium is very important for photosynthesis because
A) it is part of a pheophytin molecule
B) is a cofactor for RUBISCO
C) is directly involved in splitting water
D) A and B
E) A, B, and C
50) To make 2 molecules NADPH in photosynthesis in plants, how many photons are
needed?
A) 10
B) 8
C) 6
D) 4
E) 2
51) To produce 1 molecule of oxygen in photosynthesis in plants, how many photons are
needed?
A) 10
B) 8
C) 6

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D) 4
E) 2
52)WhenplantsareexposedtoC14labeledCO2foraveryshortperiodtime(lessthan5
seconds),whichofthefollowingwillhavethemostlabel
A) Glucose
B) Glycealdehyde3phosphate
C) 3phosphoglycerate
D) Ribulose1,5bisphosphate
E) PEP
53)UsingCO2andwaterasthestartingmaterialstomakeonemoleculeofglucosein
photosynthesis,howmanyNETATPmoleculesareneeded
A)26
B)24
C)22
D)20
E)18
54)UsingCO2andwaterasthestartingmaterialstomakeonemoleculeof
glycealdehyde3phosphateinphotosynthesis,howmanyNETNADPHmoleculesare
needed
A) 12
B) 10
C) 8
D) 6
E) 4
55)RUBISCOcatalyzedreactionneeds
A) NADPH
B) ATP
C) BothAandB
D) UTP
E) Noneoftheabove
56) Which of the enzyme is NOT important for photosynthesis in C4 plants
A) PEP carboxylase
B) Malic enzyme
C) Pyruvate phosphate dikinase
D) malate dehydrogenase
E) Fumarase
57) RUBISCO is a (an)
A) carboxylase
B) oxidase
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C) S-transferase
D) A and B
E A, B, and C
58) Regeneration of Ribulose 1, 5-bisphosphate in Calvin cycle needs
A) transketolase
B) transaldolase
C) kinase
D) A and B
E) A, B, and C
59)WhenglucoselabeledwithC14atcarbon1andcarbon6isusedtosynthesizefatty
acid,wherewillbethelabelsina16carbonfattyacid?Assumethatglucoseonlygoes
throughglycolysis,pyruvatedehydrogenase,andfattyacidbiosynthesis.Toanswerthis
question,ignoreotherpotentialfatesofglucoseandproblemsassociatedwithshuttle
intermediatesbetweendifferentlocations.
A)Everyoddnumberedcarbon
B)Everyevennumberedcarbon
C)onlythecarbonfurtherestfromcarbon1
D)onlycarbon1
E)nopartofthefattyacidmoleculewillbelabeled.
60)WhenglucoselabeledwithC14atcarbon3and4isusedtosynthesizefattyacid,
wherewillbethelabelsina16carbonfattyacid?Assumethatglucoseonlygoes
throughglycolysis,pyruvatedehydrogenase,andfattyacidbiosynthesis.Toanswerthis
question,ignoreotherpotentialfatesofglucoseandproblemsassociatedwithshuttle
intermediatesbetweendifferentlocations.
A)Everyoddnumberedcarbon
B)Everyevennumberedcarbon
C)onlythecarbonfurtherestfromcarbon1
D)onlycarbon1
E)nopartofthefattyacidmoleculewillbelabeled.
61) To make 1 molecule 16-carbon saturated fatty acid from glucose molecules, how
many NET ATP molecules are needed?
A)10
B) 7
C)5
D)2
E)noneoftheabove
62)Theelectroncarrierinphotosynthesisthathasequivalentfunctionstocytochromec
inoxidativephosphorylationis
A) NADH
B) Plastocyanin
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C) QH2
D) Pheophytin
E) Noneoftheabove
63) Neutral fats refer to
A) triacylglycerols
B) glycolipids
C) sphingolipids
D) glycerophospholipids
E) none of the above
64) Glycocholate is
A) part of a glycogen molecule
B) an important ingredient of bilesalts
C)aninhibitorforATPADPtranslocase
D)aninhibitorforelectrontransportchain
E)oneofthestatins
65)Chylomicronscontain
A)proteins
B)phospholipids
C)cholesterol
D)triacylglycerols
E)alloftheabove
66)Thefollowingstructurerefersto
A)Arg
B)MethylLysine
C)carnitine
D)Ornithine
E)Guanidine
67) Oxidation of fatty acids to acetyl-CoA is often called
A) -oxidation
B) -oxidation
C) -oxidation
D) -oxidation
E) none of the above
68) Mitochondrial ATP synthase F0
A) consists of a single subunit
B) has ATPase activity
C) is a proton channel
D) rotates in response to proton flux
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E) none of the above

69) When H2O18 was mixed with mitochondrial ATP synthase, ATP, ADP, and Pi, it was
found that Pi was labeled with O18 at multiple positions. This experiment
A) demonstrated that ATP is constantly made and hydrolyzed
B) indicated that ATP synthesis does not go through a pentavalent intermediate
C) suggested that the enzyme cannot bind Pi
D) demonstrated that ATP synthase cannot make ATP without an intact mitochondrion
E) did not provide any useful information regarding mechanisms of ATP synthesis
70) Which of the enzyme-catalyzed reaction is very similar to a reaction in fatty acid
oxidation?
A) citrate synthase
B) alpha-ketoglutarate dehydrogenase
C) aconitase
D) succinate dehydrogenase
E) none of the above
71) Glycogen synthase
A) Glucose binding leads to the dissociation of the subunits of the enzyme
B) Glucose binding can shift equilibrium to T-state
C) phosphorylation locks the enzyme to T-state
D) ATP binding can shift equilibrium to T-state
E) none of the above

72) Oxidation of odd-chain fatty acids requires

A) isomerase
B) reductase
C) decarboxylase
D) carboxylase
E) all of the above
73) Which of the following belong(s) to ketone bodies:
A) -hydroxybutyrate
B) D--hydroxybutyrate
C) succinyl CoA
D) Ethyl acetate
E) A and D
74)Completelyoxidationof6carbonfattyacid(CH3CH2CH2CH2CH2COOH)toCO2.
HowmanyNADHareproduced
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A)
B)
C)
D)
E)

8
9
10
11
12

75)Completelyoxidationof6carbonfattyacid(CH3CH2CH2CH2CH2COOH)to
CO2.HowmanyFADH2areproduced
A. 2
B. 3
C. 4
D. 5
E. 6
76)Ketonebodiescanbesynthesizedfrom
A. AcetylCoA
B. Glucose
C. Fattyacids
D. AandB
E. A,B,andC
77)Whichofthefollowinggroupofmoleculesallhaveathiogroupasthefunctional
group
A)lipoidacid,biotin,CoenzymeA
B)lipoidacid,biotin,glutathione
C)biotin,CoenzymeA,glutathione
D)lipoidacid,CoenzymeA,glutathione
E)noneoftheabove
78)WhichofthefollowingisNOTinvolvedinfattyacidbiosynthesis
A) ATP
B) NADPH
C) CoenzymeA
D) carnitine
E) MalonylcoA
79)Thekeyregulatoryenzymeforfattyacidbiosynthesisisa
A)dehydrogenase
B)carboxylase
C)transferase
D)reductase
E)hydratase
80)Adaptivecontrolinfatmetabolismrefersto:

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A)Animalsthathavefastedandarethenfedhighcarbohydrate,lowfatdiets
showmarkedincreaseinthecapacityoffattyacidsynthesiswithinafewdays
B)Animalsinitiallyfeelhungrywhenfast,butafterafewdaysfasting,theyadapt
tothelowenergyuptakeconditionandnolongerfeelhungry.
C)Animalsadapttothelowenergydietbyslowingdownmetabolismofsugar
andfat
D)Animalsadapttolowenergydietbyhydrolyzingproteinstoaminoacids,
whichprovidematerialsforgluconeogenesis
E)CandD
81)PhilosophyofAtkinsDietis
A)EatingtoomanycarbohydratescausesobesityandKetosisresultsin
reducedhunger
B)Highfatdieteventuallymakesstomachbecomesmaller
C)Highfatdietleadstopoorabsorptionofnutrients
D)Metabolizingdietaryfatstimulatesthedegradationofbodyfats
E)Adiposetissuereliesoncarbohydratesforfuelandwithoutdietary
carbohydrates,fatcellsundergoprogrammedcelldeath.
82)Themaintargetforibuprofen
A)isacyclooxygenase
B)isacylcarnitinetransferase
C)succinatedehydrogenase
Dhexokinase
E)acetylcholinereceptor
83).Statinsinterferewith
A)
B)
C)
D)
E)

HMGCoAsynthase
HMGCoAreductase
HMGCoAdehydrogenase
HMGCoAoxidase
HMGCoAlyase

84) Which of the following statement is NOT true

A) Aspartate aminotransferase needs PLP as a cofactor
B) PLP performs general acid/base catalysis in amino acid metabolism
C) Conversion of L-amino acids to D-amino acids needs PLP
D) amino acid decarboxylase often needs PLP
E) none of the above
85. Which of the following IS NOT directly involved in urea cycle
A) Arginine
B) Lysine
C) Aspartate
D) Citrulline
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E) ornithine
86. The following molecule is related to
A) Glutamate biosynthesis
B) cholesterol biosynthesis
C) fatty acid biosynthesis
D) ketone body biosynthesis
E A and D
87. The plant hormone auxin is synthesized from:
A) Phe
B) Lys
C) Trp
D) Arg
E) Glu
88 The substrate of nitric oxide synthase is:
A) Phe
B) Lys
C) Trp
D) Arg
E) Glu
89. One of the physiological functions of ketosis is:
A) to lose weight
B) another way to make glucose that is the preferred fuel for brain cells
C) to make people feel less hungry
D) to bypass oxidative phosphorylation
E) None of the above
90. Using acetyl CoA as a starting material to synthesize a 10- carbon saturated fatty
acid, how many ATP molecules are needed
A) 10
B) 8
C) 6
D) 4
E) 2
91. Using acetyl CoA as a starting material to synthesize a 10- carbon saturated fatty acid,
how many NADPH are needed
A) 10
B) 8
C) 6
D) 4
E) 2

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92. The good cholesterol usually refers to

A) HDL
B) LDL
C) IDL
D) XDL
E) none of the above

93. Which of the following is most likely involved in complex lipids

biosynthesis directly
A) GTP
B) Co-enzyme Q
C) UTP
D) CTP
E) TTP
94.Phosphorylationofglycogensynthaseisregulatedbyhormonessuchasepinephrine
thatactivatesasignaltransductionpathway.WhichofthefollowingisNOTa
componentofthesignalingpathway?
A)cAMP
B)GTP
C)ProteinkinaseA
D)Glycogenkinase
E)Noneoftheabove

95. Which of the following CANNOT be synthesized directly (one step)

from glucose-6-phosphate in liver
A) Glucose
B) Fructose-6-phosphate
C) Glucose-1-phosphate
D) 6phosphogluconolactone
E) None of the above
96.ob/obmiceanddb/dbmice
A)ob/obmiceareobese
B)db/dbmiceareobese
C)OBgeneandDBgeneworkinthesamepathway
D)AandB
E)A,B,andC

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97.BMIrefersto:
A)Bodymuscleindex
B)Bodymassindex
C)Bonemuscleindex
D)Bonemassindex
E)Bellymuscleindex
98)Whatactivityconsumesmostcaloriesperhour
A)Bicycling(10mph)
B)Running(7.5mph)
C)Standing
D)WalkingUpStairs
E)Bowling
99.Leptin

A)cansuppressappetite
B)stimulatesenergyconsumption
C)isencodedbytheOBgene
D)causesweightgainifmutated
E)alloftheabove

100.dbindb/dbmicerefersto
A)deformedbody
B)deformedbelly
C)diabetic
D)daysbeforeobese
E)noneoftheabove
TheEND

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