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ChemistryProjectAnalysisofthe
differentsamplesofmilkfor
presenceofCasein
Friday,6December2013
BlogArchive
2013(2)
ABSTRACTOF
REPORT
December(2)
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AboutMe
PatitPawanBarik
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ANALYSISOF
DIFFERENTSAMPLES
OFMILKFOR
PRESENCEOFCASEIN
A File Report submitted to :
______________
______________
Internal Examiner
______________
_______________
Head Of Chemistry Department Principal
CERTIFICATE
This is to certify that this dissertation titled
Analysis of different samples of milk for presence
of Casein was submitted by Patit Pawan
Barikto
ACKNOWLEDGE
MENT
I wish to express my deep gratitude and
sincere thanks to thePrincipal,Kendriya
Vidyalaya, Chittaranjan, W.B. for his
encouragement and for all the facilities that
he provided for this project work. I sincerely
appreciate this magnanimity by taking me into
his fold for which I shall remain indebted to
him. I extend my hearty thanks toMr.
K.Batabyal (PGT Chemistry), who guided me to
the successful completion of this project. I
take this opportunity to express my deep
sense of gratitude for his invaluable guidance,
constant encouragement, constructive
comments, sympathetic attitude and immense
motivation, which has sustained my efforts at
all stages of this project work.
DECLARATION
I do hereby declare that this project work has
been
originally carried under the guidance and
supervision
of
Mr. K. Batabyal (PGT Chemistry),
Head of Chemistry Department,
Kendriya Vidyalaya, Chittaranjan, W.B.
TABLEOF
CONTENTS
Introduction
Aim
Requirement
Theory
Procedure
Observations
Conclusion
Reference
INTRODUCTION
Milk is a food of exceptional interest. Not only is milk an
excellent food for the very young, but humans have also
adapted milk, specifically cows milk, as a food substance for
Previous
Experimental
Observations&
Results
OBSERVATIONS
Average Percentage Composition of Milk from Various
Mammals:
Source of milk
Water(%)
Minerals(%)
Proteins(%)
Fats(%)
Carbohydrates
(%)
Cow
87.1
0.7
3.4
3.9
4.9
Human
87.4
0.2
1.4
4.0
4.9
Goat
87.0
0.7
3.3
4.2
4.8
Sheep
82.6
0.9
5.5
6.5
4.5
RESULTS
The Buffalos milk has highest casein presence in all
the samples of milk. Therefore, buffalos milk is most
suitable for nutrition.
CASEIN
Casein is a phosphoprotein, which has phosphate groups are
attached to some of the amino acid side chains. Casein
exists in milk as the calcium salt, calcium caseinate. This salt
has a complex structure. It is composed of , , and
caseins which form a micelle, or a solubilized unit. Neither
the nor the casein is soluble in milk, singly or in
combination. If casein is added to either one, or to a
combination of the two, however, the result is a casein
complex that is soluble owing to the formation of the
micelle. The casein is thought to stabilize the micelle.
Since both and casein are phosphoproteins, they are
precipitated by calcium ions.
The casein protein, however, has fewer phosphate groups
and a high content of carbohydrate bound to it. It is also
thought to have all its serine and threonine residues (which
have hydroxyl groups), as well as its bound carbohydrates,
AIM
To study the quantity of Casein in different samples
of milk.
REQUIREMENTS
Beakers (250 ml)
Filter-paper
Glass rod
Weight box
Filtration flask
Filtration flask
Buchner funnel
Test tubes
Porcelain dish
Different samples of milk
1 % Acetic acid solution
CHEMICAL
REACTION
Ca2+Caseinate2- + 2 CH3COOH(aq) Casein +
(CH3COO)2Ca
THEORY
Casein is a phosphoprotein, which has phosphate groups are
attached to some of the amino acid side chains.
These are attached mainly to the hydroxyl groups of the
serine and threonine moieties. Actually, casein is a mixture
of at least three similar proteins, which differ primarily in
molecular weight and amount of phosphorus they contain
(number of phosphate groups). Casein exists in milk as the
calcium salt, calcium caseinate. This salt has a complex
structure. It is composed of , , and caseins which form a
micelle, or a solubilized unit. Neither the nor the casein
is soluble in milk, singly or in combination. If casein is
added to either one, or to a combination of the two,
however, the result is a casein complex that is soluble owing
to the formation of the micelle. The casein is thought to
stabilize the micelle. Since both and casein are
phosphoproteins, they are precipitated by Calcium ions.
The casein protein, however, has fewer phosphate groups
and a high content of carbohydrate bound to it. It is also
thought to have all its serine and threonine residues (which
have hydroxyl groups), as well as its bound carbohydrates,
PROCEDURE
1. A clean dry beaker has been taken, followed by
putting 20 ml of cows milk into it and adding 20 ml of
saturated ammonium sulphate solution slowly and with
stirring. Fat along with Casein was precipitate out.
2. The solution was filtered and transferred the
precipitates in another beaker. Added about 30 ml of
water to the precipitate. Only Casein dissolves in water
forming milky solution leaving fat undissolved.
3. The milky solution was heated to about 40oC and add
1% acetic acid solution drop-wise, when casein got
precipitated.
4. Filtered the precipitate, washed with water and the
precipitate was allowed to dry.
5. Weighed the dry solid mass in a previously weighed
watch glass.
6. The experiment was repeated with other samples of
milk.
OBSERVATIONS
According to above experiment :
Sl. No.
Source of Milk
CONCLUSION
The __________ milk has highest value of the dried solid
mass i.e. the composition of Casein is highest in the sample
of ________ milk.
SOLUTIONTOTHE
ANALYSIS
The ________ milk has the highest presence of casein in
milk. Therefore, ________ milk is the best for nutrition.
REFERENCES
Wikipedia
Encyclopedia Encarta
Britannica
icbse.nic.in
Practical Chemistry books
Nocomments:
ABSTRACTOF
REPORT
ANALYSISOF
DIFFERENTSAMPLES
OFMILKFOR
PRESENCEOFCASEIN
A File Report submitted to :
______________
______________
Internal Examiner
______________
_______________
Head Of Chemistry Department Principal
CERTIFICATE
This is to certify that this dissertation titled
Analysis of different samples of milk for presence
of Casein was submitted by Patit Pawan
Barikto
Head of Chemistry Department of
Kendriya Vidyalaya, Chittaranjan, W.B.
carried under guidance and supervision during
the
Academic year 2013-2014.
ACKNOWLEDGE
MENT
I wish to express my deep gratitude and
sincere thanks to thePrincipal,Kendriya
Vidyalaya, Chittaranjan, W.B. for his
encouragement and for all the facilities that
he provided for this project work. I sincerely
appreciate this magnanimity by taking me into
his fold for which I shall remain indebted to
him. I extend my hearty thanks toMr.
K.Batabyal (PGT Chemistry), who guided me to
the successful completion of this project. I
take this opportunity to express my deep
sense of gratitude for his invaluable guidance,
constant encouragement, constructive
comments, sympathetic attitude and immense
motivation, which has sustained my efforts at
all stages of this project work.
DECLARATION
I do hereby declare that this project work has
been
originally carried under the guidance and
supervision
of
Mr. K. Batabyal (PGT Chemistry),
Head of Chemistry Department,
Kendriya Vidyalaya, Chittaranjan, W.B.
TABLEOF
CONTENTS
Introduction
Aim
Requirement
Theory
Procedure
Observations
Conclusion
Reference
INTRODUCTION
Milk is a food of exceptional interest. Not only is milk an
excellent food for the very young, but humans have also
adapted milk, specifically cows milk, as a food substance for
Previous
Experimental
Observations&
Results
OBSERVATIONS
Average Percentage Composition of Milk from Various
Mammals:
Source of milk
Water(%)
Minerals(%)
Proteins(%)
Fats(%)
Carbohydrates
(%)
Cow
87.1
0.7
3.4
3.9
4.9
Human
87.4
0.2
1.4
4.0
4.9
Goat
87.0
0.7
3.3
4.2
4.8
Sheep
82.6
0.9
5.5
6.5
4.5
RESULTS
The Buffalos milk has highest casein presence in all
the samples of milk. Therefore, buffalos milk is most
suitable for nutrition.
CASEIN
Casein is a phosphoprotein, which has phosphate groups are
attached to some of the amino acid side chains. Casein
exists in milk as the calcium salt, calcium caseinate. This salt
has a complex structure. It is composed of , , and
caseins which form a micelle, or a solubilized unit. Neither
the nor the casein is soluble in milk, singly or in
combination. If casein is added to either one, or to a
combination of the two, however, the result is a casein
complex that is soluble owing to the formation of the
micelle. The casein is thought to stabilize the micelle.
Since both and casein are phosphoproteins, they are
precipitated by calcium ions.
The casein protein, however, has fewer phosphate groups
and a high content of carbohydrate bound to it. It is also
thought to have all its serine and threonine residues (which
have hydroxyl groups), as well as its bound carbohydrates,
AIM
To study the quantity of Casein in different samples
of milk.
REQUIREMENTS
Beakers (250 ml)
Filter-paper
Glass rod
Weight box
Filtration flask
Filtration flask
Buchner funnel
Test tubes
Porcelain dish
Different samples of milk
1 % Acetic acid solution
CHEMICAL
REACTION
Ca2+Caseinate2- + 2 CH3COOH(aq) Casein +
(CH3COO)2Ca
THEORY
Casein is a phosphoprotein, which has phosphate groups are
attached to some of the amino acid side chains.
These are attached mainly to the hydroxyl groups of the
serine and threonine moieties. Actually, casein is a mixture
of at least three similar proteins, which differ primarily in
molecular weight and amount of phosphorus they contain
(number of phosphate groups). Casein exists in milk as the
calcium salt, calcium caseinate. This salt has a complex
structure. It is composed of , , and caseins which form a
micelle, or a solubilized unit. Neither the nor the casein
is soluble in milk, singly or in combination. If casein is
added to either one, or to a combination of the two,
however, the result is a casein complex that is soluble owing
to the formation of the micelle. The casein is thought to
stabilize the micelle. Since both and casein are
phosphoproteins, they are precipitated by Calcium ions.
The casein protein, however, has fewer phosphate groups
and a high content of carbohydrate bound to it. It is also
thought to have all its serine and threonine residues (which
have hydroxyl groups), as well as its bound carbohydrates,
PROCEDURE
1. A clean dry beaker has been taken, followed by
putting 20 ml of cows milk into it and adding 20 ml of
saturated ammonium sulphate solution slowly and with
stirring. Fat along with Casein was precipitate out.
2. The solution was filtered and transferred the
precipitates in another beaker. Added about 30 ml of
water to the precipitate. Only Casein dissolves in water
forming milky solution leaving fat undissolved.
3. The milky solution was heated to about 40oC and add
1% acetic acid solution drop-wise, when casein got
precipitated.
4. Filtered the precipitate, washed with water and the
precipitate was allowed to dry.
5. Weighed the dry solid mass in a previously weighed
watch glass.
6. The experiment was repeated with other samples of
milk.
OBSERVATIONS
According to above experiment :
Sl. No.
Source of Milk
CONCLUSION
The __________ milk has highest value of the dried solid
mass i.e. the composition of Casein is highest in the sample
of ________ milk.
SOLUTIONTOTHE
ANALYSIS
The ________ milk has the highest presence of casein in
milk. Therefore, ________ milk is the best for nutrition.
REFERENCES
Wikipedia
Encyclopedia Encarta
Britannica
icbse.nic.in
Practical Chemistry books
Nocomments:
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