2nd ICB-Pharma ProgrammeAndAbstractBook

INTERNATIONAL CONFERENCE
ICB PHARMA II
PROGRAMME
and
ABSTRACT BOOK
Current Breakthrough in
Pharmacy Materials and Analyses
Faculty of Pharmacy
Universitas Muhammadiyah Surakarta
2015
Pharmacy Faculty, Universitas Muhammadiyah Surakarta

Surakarta, Indonesia
Chairman of Editors
Erindyah Retno Wikantyasning, Ph.D., Apt.

Tanti Azizah Sujono, M.Sc., Apt.
Team of Pharmaceutical Technology
Anita Sukmawati, Ph.D., Apt.

Suprapto, M.Sc.,Apt.
Erindyah Retno Wikantyasning, Ph.D., Apt.
Gunawan Setiyadi, M.Sc., Apt.
Team of Pharmacology and Microbiology
Azis, Saifudin, Ph.D., Apt.

Arifah Sri Wahyuni, M.Sc., Apt.
Ratna Yuliani, M.Biotech.St.
Tanti Azizah Sujono, M.Sc., Apt.
Team of Clinical and Community Pharmacy
Dra. Nurul Mutmainah, M.Si., Apt.

Zakky Cholisoh, M.Clin. Pharm., Ph.D., Apt.
Hidayah Karuniawati, M.Sc. Apt.
Team of Pharmaceutical Chemistry
Dedi Hanwar, M.Si., Apt.

Dr. Muhammad Dai, M.Si., Apt.
Dr. Muhtadi Ibrahim, M.Sc.
Broto Santoso, M.Sc., Apt.
Andi Suhendi, M.Sc., Apt.
Ika Trisharyanti, M.Sc., Apt.
Team of Molecular Biology
Agus Purnomohadi, M.Sc.

Maryati, Ph.D., Apt.
Copyright 2015
Copyright in compilers and reserved
Design cover: Publication and Documentation
Team Layout: Secretary and IT Team
Published by:
Muhammadiyah University Press
Jl. A. Yani Pabelan Tromol Pos I Kartasura Surakarta 57102
Telp. (+62 271) 717417-172, E-mail: muppress@yahoo.com
ii | P a g e
ICB Pharma II 31 October 2015

Current Breakthrough in Pharmacy Materials and Analyses
PREFACE
Its my great pleasure to welcome you to the 2nd International Current

Breakthrough (ICB)-Pharma Symposium 2015 in Solo Indonesia which will be held on 31
October, 2015 under the auspices of Universitas Muhammadiyah Surakarta.
ICB-Pharma 2015 will feature a theme of Current Breakthrough in Pharmacy
Material and Analyses and will consist of morning and afternoon sessions. There will be
plenary lectures and session lectures given by several invited speakers from Singapore,
Japan, as well as from Indonesia, and also selected oral presentations of the submitted
papers. The poster session from various fields of pharmaceutical sciences will take place
nearby.
The ICB-Pharma will be directed for a tradition and in the future will be nurtured
as a well-known scientific symposium in disseminating the breakthrough and novel
technology in pharmacy materials. This purpose will be able to achieve by encouragement
of national and international academic institution partners and supports from the
industrial partners, Indonesian Pharmacist Association (Ikatan Apoteker Indonesia, IAI),
colleagues and from the organizing committee. Moreover, I do hope and believe that, the
2nd ICB-Pharma will offer great opportunities for the scientists to meet and discuss recent
topics in the field of material and pharmaceutical science and bring the academics, health
professionals and industries together for sharing their experiences to solve current
problems and challenges in practice.
Warm regards,
Azis Saifudin, PhD, Apt.

Chair of ICB-Pharma Symposium

iii | P a g e
PREFACE
This conference is held to disseminate current methods which provide advanced

materials and methods in pharmacy. This is a good media for those who are engaged in
academic, industrial, regulatory fields to conduct social interactions, to share their
findings, to communicate bottle neck surrogates, and to seek the possibilities for
collaborations. We are quite humble to recognize our weakness in running all agendas.
Hence, from bottom of our heart we ask apologizes from all participants for any service,
lack facilities, low response, etc. However, we must be persistent and ensuring our
positive contribution toward scientific society especially at the attempt to develop
capacity building of pharmaceutical sciences in Indonesia. Thus, we will run ICB Pharmacy
III next year.
To all participants, I wish an inspiring moment in Solo city, a city where was born a
national leader and city of heritage!
Azis Saifudin, PhD, Apt.

Dean of Faculty of Pharmacy
iv | P a g e

TABLE OF CONTENTS
PREFACE.................................................................................................................................................................. iii
TABLE OF CONTENTS .......................................................................................................................................... v
PROGRAMME .......................................................................................................................................................... ix
ORAL PRESENTER ROOM................................................................................................................................... x
LIST OF PARTICIPANT NON PRESENTERS ................................................................................................ xi
LIST OF ORAL PRESENTERS ......................................................................................................................... xvi
LIST OF POSTER PRESENTERS ..................................................................................................................xviii
SPEAKER ................................................................................................................................................................... 1
A-PHARMACEUTICAL TECHNOLOGY ............................................................................................. 4
A001
CHARACTERISTICS TESTING OF MICROCRYSTALLINE CELLULOSE FROM NATA

DE COCO COMPARED TO AVICEL pH 101 AND AVICEL pH 102
Adi Yugatama1*, Laksmi Maharani2, Hening Pratiwi2, Lingga Ikaditya3 .......................................... 4

A002
PREPARATION OF ARTIFICIAL SALIVA FORMULATION
Andi Sri Suriati Amal1*, Samsinah Hj. Hussain2, Mohd. Amin Jalaluddin3 ....................................... 5
A003
FORMULATION OF ETHANOL EXTRACT OF TUNJUK LANGIT (Helminthostachys

zaylanica) RHIZOME AS TABLET DOSAGE FORM BY WET GRANULATION
METHOD
Fitrya1*, Najma Annuria Fithri1, Budi Untari1, Aprililianti1................................................................... 6

A004
EFFECT COGRINDING ON THE PHYSICAL CHARACTERISTIC OF BINARY

MIXTURE OF PIOGLITAZONE HCl AND METFORMIN HCl
Iskandar Zulkarnain1*, S. N. Soewandhi1, S. Wikarsa2 ............................................................................. 7

A005
PREPARATION AND CHARACTERIZATION OF SUBMICRON PARTICLES OF PLGA

INCORPORATING RIFAMPIN USING EMULSION SOLVENT DIFFUSION METHOD
Mardiyanto1* ............................................................................................................................................................ 8
A006
LIQUID BATH SOAP FORMULATION AND ANTIBACTERIAL ACTIVITY TEST

AGAINST Staphylococcus aureus OF KECOMBRANG (Etlingera elatior (Jack)
R.M.Sm.) FLOS EXTRACTS
Lilis Handrayani1, Ratih Aryani1*, Indra1...................................................................................................... 9

A007
THE INFLUENCE OF EGGPLANT PEEL EXTRACT (Solanum melongena L.) AS

ANTIOXIDANT IN LOTION DOSAGE FORM
Sholichah Rohmani1* ......................................................................................................................................... 10

A008
OPTIMIZING COMBINATION OF SAMBILOTO HERBAL WATER FRACTION AND

SALAM LEAF WATER FRACTION AS ANTI-INFLAMMATION
Raymond Harris Mustafa1, Lannie Hadisoewignyo1*, Martha Ervina1, Lisa Soegianto1,

Wahyu Dewi Tamayanti1 .............................................................................................................. 11

v|P a g e
B-PHARMACOLOGY AND MICROBIOLOGY ................................................................................. 12

B001
THE POTENTIAL OF THE BLACK RICE BRAN EXTRACT AS ANTIDIABETIC AGENT
Arifah Sri Wahyuni1*, Rima Munawwaroh1, Esthi Utaminingsih1, Novita Sari1 ........................ 12
B002
ANTIOXIDANT ACTIVITIES OF NANOEMULSION CONTAINING EXTRACT OF

SAMBILOTO (Andrographis paniculata (Burm F.) Ness.) AND MENIRAN
(Phyllanthus niruriL.) IN ALLOXAN- INDUCED DIABETIC RATS
Erindyah R Wikantyasning1*, Nurcahyanti Wahyuningtyas1, Muhammad Dai1, Doni

Wibowo1............................................................................................................................................... 13
B003
ACUTE TOXICITY STUDY OF NANOEMULSION CONTAINING SAMBILOTO

(Andrographis paniculata) LEAVES AND MENIRAN (Phyllanthus niruri) HERBS
EXTRACT IN WISTAR RATS
Erindyah R. Wikantyasning1*, Nurcahyanti Wahyuningtyas1, Muhammad Dai1, Febby

Lovita Sari1 .......................................................................................................................................... 14
B004
ANTI-INFLAMMATORY ACTIVITY TEST OF CHRISTMAS PALM (Adonidia merrillii

(Becc.) Becc.) SEED EXTRACT IN MALE WISTAR RATS (Rattus norvegicus)
Herlina1*, Fitrya1, Fithri Najma A1, Rahmawati Dwi Shafarina1 ....................................................... 15

B005
THE EFFECT OF GIVING GLUCOMANNAN PORANG TUBER (Amorphophallus

oncophyllus Prain ex Hook. F.) ON SGPT AND SGOT LEVELS OF MALE WISTAR
RATS BLOOD INDUCED BY PARACETAMOL
Intan Martha Cahyani1*, Bekti Nugraheni1 ............................................................................................... 16

B006
THE PROFILE OF MDA (malondialdehyde) LEVEL IN RATS GIVEN EXTRACT OF

THYMI HERBS (Thymus vulgaris [L.])
Ken Inayati Latifa1*, Tanti Azizah Sujono1, Ika T. Dian Kusumowati1 ........................................... 17
B007
PRE-CLINICALSTUDY OF Cr (III) BASED HYPOGLICEMIC SUPPLEMENT IN-TYPE

2 DIABETIC RATS
Kun Sri Budiasih1*, Kartika Ratna Pertiwi1............................................................................................... 18

B008
SCREENING ANTIBACTERIAL POTENCY OF ENDOPHYTIC FUNGI METABOLITE

OF MANGOSTEEN (Garciniamangostana L.) LEAF
Lisa Soegianto1*, Martha Ervina1, Kevin Widjaja1, Angela Violita1 ................................................. 19

B009
ACTIVITIES OF THE COMBINED EXTRACTS OF TEMPUYUNG (Sonchus arvensis)

AND BLACK CUMIN (NIGELLA SATIVA) AGAINST XANTHINE OXIDASE
INHIBITION ON HYPERURICEMIC MICE
Muhtadi1*, Nurcahyanti Wahyuningtyas1, Andi Suhendi1, Septi Heryani1 .................................. 20

B010
ANTIBACTERIAL ACTIVITY OF COMBINATION OF CHLORAMPHENICOL AND

ETHANOLIC EXTRACT OF PACAR AIR (Impatiens balsamina) LEAVES AGAINST
Escherichia coli AND Shigella sonnei
Ratna Yuliani1*, Agung Cokro Prabowo1, Yeni Maisyah1..................................................................... 21

B011
ANTIHYPERCHOLESTEROLEMIC EFFECT OF Murbei (Morus alba L.) LEAVES

AND ITS COMBINATION WITH SIMVASTATIN
IN RATS INDUCED BY
PROPYLTIOURACIL AND HIGH FAT DIET
Tanti Azizah Sujono1*, Haryoto1, Ratna Kartikasari1, Laily Ieda Quntari1 ................................... 22
vi | P a g e

B012
ANALGESIC EFFECT OF ETHANOLIC EXTRACT RED DRAGON FRUIT ( Hylocereus

polyrhizus Cortex) PEEL ON MALE MICE SWISS WEBSTER WITH WRITHING
REFLEX METHOD
Westi Fajrin Bayu Nugrahaini1*, Tanti Azizah Sujono1 ........................................................................ 23

B013
EFFECTS OF TURMERIC ETHANOLIC EXTRACT ON TRIMETHYLTIN-INDUCED

OXIDATIVE STRESS ON SPRAGUEY DAWLEY RATS
Sapto Yuliani1*, Mustofa Mustofa2, Ginus Partadiredja2...................................................................... 24

B014
ANTIDIABETIC ACTIVITY OF NANOEMULSION CONTAINING EXTRACT OF

SAMBILOTO (Andrographis paniculata (BURM F.) NESS.) AND MENIRAN
(Phyllanthus niruri L.) IN ALLOXAN-INDUCED DIABETIC RATS .................................. 25
Erindyah R. Wikantyasning1*, Nurcahyanti Wahyuningtyas1, Muhammad Dai1, Fajar

Kholikul Amri1 ................................................................................................................................... 25
C- CLINICAL AND COMMUNITY PHARMACY .............................................................................. 26
C001
OVERVIEW ABOUT ANTIBIOTICS PRESCRIBING IN URINARY TRACT INFECTION

ROEMANI SEMARANG HOSPITALS INPATIENTS
Hening Pratiwi1* .................................................................................................................................................. 26

C002
RATIONALITY TREATMENT OF ANTIBIOTICS FOR TREATMENT OF DIARRHEA

IN ADULT PATIENTS IN THE INPATIENT INSTALLATION OF HOSPITAL X
SURAKARTA IN 2014
Ida Ayu Pebrina1*, Suharsono1, Suprapto1 ................................................................................................ 27

C003
CLINICAL IMPROVEMENT AFTER CEPHALOSPORINE THERAPY ON CHILDREN

WITH TYPHOID FEVER
Rasmaya Niruri1*, N.P. Yulia Purnami1, Julius D.Tansale2,3, I.B.Eka Erlangga3.......................... 28

C004
TRANSAMINITIS ASSOCIATED WITH HIGH DOSE METHOTREXATE AND 6MERCAPTOPURIN IN CHILDREN WITH ACUTE LYMPHOBLASTIC LEUKEMIA
Rasmaya Niruri1*, K. Trisna Komalasari1, Ketut Ariawati2 ................................................................ 29

C005
TREATMENT AND COST ANALYSIS OF DIARRHEA PATIENTS OF INPATIENT

INSTALLATION OF RSUD dr. MOEWARDI SURAKARTA BY BPJS PROGRAM IN
2014
Saktya Ayu Donna P1*, Suharsono1, Suprapto1........................................................................................ 30

D-PHARMACEUTICAL CHEMISTRY ............................................................................................... 31
D001
ANTIOXIDANT ACTIVITY OF ETHYL ACETATE EXTRACT OF PARKIA SEED AND

POD (Parkia speciosa Hassk.) BY DPPH (1,1-DIPHENYL-2- PICRYLHYDRAZYL)
METHOD .............................................................................................................................................. 31
Indri Kusuma Dewi1*, Agus Winarso1 ......................................................................................................... 31

D002
A CLICK-TYPE COUPLING REACTION BETWEEN THIOAMIDES AND SULFONYL

AZIDES
AS
A
VERSATILE
APPROACH
TO
GENERATE
NEW
PHARMACOLOGICALLY ACTIVE COMPOUNDS ................................................................... 32
Muhammad Aswad1, Junya Chiba1*,Yasumaru Hatanaka1, Takenori Tomohiro1 ..................... 32

D003
ANTIBACTERIAL COMPOUND PRODUCED BY A SOIL BACTERIA ISOLATED FROM

RIZHOSPHERE OF Zingiber officinale....................................................................................... 33

vii | P a g e
Nanik Sulistyani1,2*, Yosi Bayu Murti3, Jaka Widada4, Mustofa5 ....................................................... 33

E-MOLECULAR BIOLOGY .................................................................................................................. 34
E001
CYTOTOXIC ACTIVITY OF POLAR, SEMIPOLAR, AND NON POLAR FRACTION OF

ETHANOL EXTRACT OF SALA PLANTS LEAVES (Cynometra ramiflora Linn.)
AGAINTS WiDr CELL
Haryoto1*, Anis N. Irjayanti1, Tanti Azizah Sujono1, Muhtadi1, Andi Suhendi1 .......................... 34
E002
CHALCONES DERIVATIVE WITH BROMO SUBSTITUENT INDUCES APOPTOSIS IN

HeLa CELLS
Retno Arianingrum1*, Indyah Sulistyo Arty1 ............................................................................................ 35

E003
SUB-CLONING OF ads GENE INTO pETDUET1_cyp FOR CO-EXPRESSION IN

ESCHERICHIA COLI
Imam A. Wicaksono1, Tresna Lestari2*, Evi U. Ulfa3, Catur Riyani1, Elfahmi1 ............................. 36
CV OF SPEAKER ................................................................................................................................................... 37
LIST OF COMMITTEES ...................................................................................................................................... 40
MAP OF FACULTY OF PHARMACY ............................................................................................................... 42
LIST OF TAXI AND HOTELS ............................................................................................................................ 43
viii | P a g e

PROGRAMME
Venue:
Auditorium Moh. Djazman, Universitas Muhammadiyah Surakarta
Jl. Ahmad Yani, Tromol Pos I, Pabelan, Surakarta 57162 Indonesia
Session:
Morning Session
07.00-08.00
Registration for participant
08.00-08.30
Opening Ceremony
08.30-09.00
Morning Coffee Break
09.00-09.45
Speaker 1
Prof. Jackie Ying (Executive Director, Institute of Bio-engineering and
Nanotechnology)
Topic: "Current status on nano medicine and nano devices"
09.45-10.30
Speaker 2
Assoc. Prof. Takenori Tomohiro (University of Toyama, Japan).
Topic: "Photoaffinity labeling-based target identification of bioactive
molecules"
10.30-11-15
Speaker 3
Dr. Wangsa Tirta Ismaya (Scientist/Head section of Recombinant
therapeutic proteins, PT. Dexa Medica).
Topic: "Current status of recombinant therapeutic proteins and local
issues related"
11.15-12.00
Discussion
12.00-13.00
Lunch break
Afternoon Session
13.00-13.30
Poster Session
13.30-16.00
Oral Presentation Session
16.00-16.30
Closing Ceremony

ix | P a g e
ORAL PRESENTER ROOM

Room
K4-A
Code
Presenter
A001
ADI YUGATAMA
A003
FITRYA
A005
MARDIYANTO
A006
RATIH ARYANI
Moderator
Gunawan Setiyadi, M.Sc.,

Apt.
A008
K4-B
K4-C
K4-D
x|P a g e
C001
HENING PRATIWI
C003
RASMAYA NIRURI
D001
INDRI KUSUMA DEWI
D002
MUHAMMAD ASWAD
B001
ARIFAH WAHYUNI
B004
HERLINA
B007
KUN BUDIASIH
B009
MUHTADI
B011
TANTI AZIZAH
B008
LISA SOEGIANTO
B010
RATNA YULIANI
E001
HARYOTO
E002
RETNO ARIANINGRUM
E003
TRESNA LESTARI
Andi Suhendi, MSc., Apt.
Dedi Hanwar, M.Si., Apt.
Maryati, Ph.D., Apt.

LIST OF PARTICIPANT NON PRESENTERS
No
Name
Institution
ARDIANSAH
UMS
HIMYATUL HIDAYAH
PRODI MAGISTER FARMASI UMS
MARIA ULFA
MARIA ULFA
ANANDA FARMA
ABDUL RONI
UMS
MUSTAKIM MASNUR
UMS
DITA MARINA LUPITANINGRUM
UMI KHOLIFAH.S.FARM.,APT
APOTEK KARUNIA FARMA
APOTEK WALI SEHAT SEMARANG
11
LENI HERLINA WIDIASARI, S.SI.,APT.

DHIGNA LUTHFIYANI CITRA
PRADANA
YUSNITA APRILIYANA
12
NOVI DIANASARI, S.FARM, APT.
APOTIK KONDANG WARAS
13
DEVI UTAMI YULISTYANTI S. FARM.,

APT
ELLA SKIN CARE SOLO
14
DIKA HERNAWATI S.FARM., APT
ELLA SKIN CARE SOLO
15
ARIS SETIAWAN
KLINIK INSANI MEDICA
16
UNGKI PRASETYO
MARIA RINA DWI
SUSILOWATI.,S.SI.,APT
DRA. DWI MURTINGHASTUTI,APT
AGNES RINA SRI MURWANI,
S.SI.,APT
DWI KARTIKA SANTI
KLINIK RETNO
APOTEK SUMBER SEHAT BOYOLALI
24
SRI HARTINI, S.FARM.APT

NUR SEKTI HIDAYAT RAHMAWATI
S. FARM., APT.
DIAZ VEGA AKHIRUNNISA, S.FARM.,
APT
ANNISA RIZKI MURDIYANI
25
UMI KHOLIFAH.S.FARM.,APT
APOTEK KARUNIA FARMA
26
HANDIKA DESI YANOTAMA
SMK ASSHODIQIYAH SEMARANG
27
BPOM RI
29
RETNO HARI WAHYUNI

CAESNAN MARENDRA GRAHAN
LEDITTO
SARAH PUSPITA ATMAJA
30
MEILANA SUSANTI
APOTEK HARJOSARI FARMA
31
ULFAHTUL LAELI
APOTEK DAWA FARMA
32
INAYATUL HIDAYATI
APOTEK SERAYA
33
LULU BACHRIYAH
APOTEK MUTIARA
10
17
18
19
20
21
22
23
28
UNIVERSITAS JAMBI
APOTEK SIDAPURNA
PT. GRAHA FARMA

PT. GRAHA FARMA
PT. GRAHA FARMA
RS PKU MUHAMMADIYA KARTASURA
UMS
LBC WONOSOBO
PT. DAYA MUDA AGUNG
UNIVERSITAS KRISTEN IMMANUEL

AIRLANGGA UNIVERSITY

xi | P a g e
No
Name
Institution
34
MARLIN TIGOR S.SI. APT
APOTEK PUTRID
35
APOTIK WALI SEHAT
37
LENI HERLINA WIDIASARI SSI.,APT

ARRYSKA AYU PERMATASARI
S.FARM.,APT
MALICHATUN
38
RIZAMDHANI
APOTEK KIRANI
39
IAI PC KAB. TEGAL
41
LINNA MARLINNA S.SI, APT

HENDRA ANTAN DJAYA, S.SI, MBA,
APT
QUROTUL AINI, S.FARM.,APT
42
IRA RAHMIYANI
36
40
43
44
FRANSISCA MEINDRIA NARASTY,

S.FARM., APT.
DESTANTIA NADYA AMANTHA
S.FARM., APT
IAI KAB TEGAL

APOTEK KAROMAH
IAI PC KAB. TEGAL

APOTEK MITRA MEDIKA
STIKES BAKTI TUNAS HUSADA
TASIKMALAYA
PT. GRAHA FARMA SURAKARTA
STIFAR YAYASAN PHARMASI
SEMARANG
STIFAR YAYASAN PHARMASI
SEMARANG
UNIVERSITAS GADJAH MADA
45
TYAS SARININGRUM S.FARM., APT
46
48
CANDRA EKA PUSPITASARI

DHIGNA LUTHFIYANI CITRA
PRADANA
FARIDHA CYNTHIA DHEWANTI
49
SITI CHOTIAH
UMS
50
AMIRA
UMS
51
TRI YOGO WIBOWO
UMS
52
AHMAD RIFQI KURNIA
UMS
53
ABDUL HADI
UMS
54
NUR ALFIAWATI
UMS
55
CHOIRUL MA'ARIF
UMS
56
RAFA ENBUN RELIGIA
UMS
57
SOVY SAPTA NUARI PRAMOLIS
UMS
58
NAUFAL SATRIA HUTOMO
UMS
59
HESTU PUTRI MITAYANI
UMS
60
SANGGITA AYU IKASARI
UMS
61
FAHMI AZHARI
UMS
62
DYAH RISWARI PITALOKA
UMS
63
ALIFAH ANASTYA DINI
UMS
64
RANI UTAMI WIDYANINGRUM
UMS
65
MARHAMAH NUR AZIZAH
UMS
66
DRAJAT TRI WAHYUDI
UMS
67
LITA RAHIMA OENSJAR
UMS
68
EKHWAN TRIS WANTO

ANDHIKA RIZKY GILANG
MAHAPUTRA
ULITYATINING TIASARI
UMS
47
69
70
xii | P a g e
UNIVERSITAS JAMBI
UMS
UMS
UMS

No
Name
Institution
71
AMALIYAH DINA ANGGRAENI
UMS
72
WIWIK NURYANI
UMS
73
AGUNG COKRO PRABOWO
UMS
74
ESTUNINGTYAS AYU HAPSARI
UMS
75
RISQIE CAHYANING KUSUMASARI
UMS
76
IRHAMADI MALIK
UMS
77
YENI MAISYAH
UMS
78
UMUL BAROROTUY SYAMS
UMS
79
UMI NURHAYATI
UMS
80
RIZKI OCTAVIANA
UMS
81
BAIQ SUPRAMONIKA
UMS
82
MUTMAINNAH
UMS
83
HAZRINI TANJUNG SARI
UMS
84
RENY WIDYA ESTUTI
UMS
85
ABULKHAIR ABDULLAH
UMS
86
SHIFA SILFIA
UMS
87
NURYATI KUMAN
UMS
88
FITRIANISA FATHUROHMAH
UMS
89
IMROATUL CHASANAH
UMS
90
MARWIANI ARUM SARI
UMS
91
RAHAYU DWI KURNIAWATI
UMS
92
ALISA PRIHARSI
UMS
93
JANIKA SUJI KUSUMAWARDANI
UMS
94
AYU ANGGRAENY
UMS
95
EMAMATUL KHUTSIYAH
UMS
96
BUNGA INTAN SAVITRI
UMS
97
ANNIE RAHMATILLAH
UMS
98
ISTIQAMATUSH SHOLIHAH
UMS
99
ANGGRIANA ARISTYA
UMS
100
ISNAINI NUR HIDAYAH
UMS
101
NAHYATU SUFIAH
UMS
102
RAUDAH PUTERI ALMINA
UMS
103
ADIVA TANTYAS AURORA
UMS
104
FADILA KHOIRUNNISA
UMS
105
SARI WIJAYANTI
UMS
106
INTAN RIA NURJANAH
UMS
107
SITI PURWATI
UMS
108
SUNJAWA ALIF SAPUTRI
UMS
109
KURNIA PUNGKY ASMORO
UMS
110
NIKEN DWI MULYASARI
UMS

xiii | P a g e
No
Name
Institution
111
AISYAH PUTRIANI
UMS
112
FADHILA DIAH SUMINAR
UMS
113
SEKAR PUJI UTAMI
UMS
114
WITRI DYAH MEILANI
UMS
115
NINA NURMITA HABSARI
UMS
116
VERANTIKA DEA INDRASWARI
UMS
117
TRI AGUS SAROSO
UMS
118
YUDA MARSONO
UMS
119
AULIA ANNUR AISYIAH
UMS
120
ADHI WARDHANA AMRULLAH
UMS
121
FEBRIANNA SURYANINGTYAS
UMS
122
ANINDYA SETYOWATI
UMS
123
NOVITA SARI
UMS
124
RATNA KARTIKASARI
UMS
125
ESTHI UTAMININGSIH
UMS
126
YENY DWI NOVITASARI
UMS
127
NENI LUGKI NIAN TARY
UMS
128
SALLY ASTYA UTAMI
UMS
129
TANTIN HAYU SURYA
UMS
130
THORIQ MAHMUD
UMS
131
RAKIH YUSMA RANGGA
UMS
132
HENDRA YUANA
UMS
133
GITA AYU PRADINA
UMS
134
BERNADI WICAKSONO
UMS
135
ROSMA FAUZIAH
UMS
136
TESAR ZULMI ANTORO
UMS
137
ISTI SETIA HAPSARI
UMS
138
BENY DWI HATMOKO
UMS
139
FIKA RIZQIYANA
UMS
140
ANGGITA SEKAR ARUMSASI
UMS
141
DIAN YULISTIA ASTRI
UMS
142
DIAN AYU ARA ARA ARTHASARI
UMS
143
EKA PRASNAPARAMITA W
UMS
144
RIZKA ASTIKAH FITRIANA
UMS
145
NUNGKY ASMARANING WAHYONO
UMS
146
DEVI AMBARRINI WAHYUNINGTYAS
UMS
147
DESTY RIRIN ROHMAWATI
UMS
148
EKA PRADITA PUTRI
UMS
149
AKHMAD AMINUR RIZKI
UMS
150
LAILY IEDA QUNTARI
UMS
151
MUHAMMAD PRIYADI
UMS
xiv | P a g e

No
Name
Institution
152
MANGGAR ARUM SHINTYA M.
UMS
153
DWI SETIANINGRUM MAKUNTI
UMS
154
TITIS MUTALIKAH
UMS
155
MARIA ULFA
UMS
156
EKA SETIANISA
UMS
157
ORLAN PAKAMBANAN
UMS
158
APRILYA SRI RACHMAYANTI
UMS
159
DIAN RAHMAWATI
160
DWI SARYANTI
161
SALSABIELA DWIYUDRISA SUYUDI
UMS
AKADEMI FARMASI NASIONAL
SURAKARTA
UMS
162
NORA FAUZIAH
UMS
163
RINI YULIANA
UMS
164
LILIS FITRIATUN NISA
UMS
165
EDI SUTARMANTO
166
NURLELA IKAWATI
167
INES NURFITRIANA
168
170
WULAN PRIATIWI
KATHLEEN APRIANA
KRISTININGRUM JAHAMOU
YULI FITRIANA
171
HALIDA SURYADINI
UMS
172
PUTRI RAMDANIAH
173
CHANDRA EKA PUSPITASARI
UGM
169
UMS

xv | P a g e
LIST OF ORAL PRESENTERS

No
1
Code
A001
Presenter
ADI YUGATAMA
Institution
UNIV.
SEBELAS
MARET
A003
FITRYA
SRIWIJAYA
UNIVERSITY
A005
MARDIYANTO
SRIWIJAYA
UNIVERSITY
A006
RATIH ARYANI
STIKES BAKTI
TUNAS
HUSADA
A008
RAYMOND
HARRIS
MUSTAFA
B001
B004
ARIFAH
WAHYUNI
HERLINA
WIDYA
MANDALA
SURABAYA
CATHOLIC
UNIVERSITY
UMS
B007
KUN BUDIASIH
UNY
B008
LISA SOEGIANTO
10
B009
MUHTADI
WIDYA
MANDALA
SURABAYA
CATHOLIC
UNIVERSITY
UMS
11
B010
RATNA YULIANI
UMS
12
B011
TANTI AZIZAH
UMS
xvi | P a g e
SRIWIJAYA
UNIVERSITY
Title
CHARACTERISTICS TESTING OF
MICROCRYSTALLINE CELLULOSE FROM
NATA DE COCO COMPARED TO AVICEL pH
101 AND AVICEL pH 102
FORMULATION OF ETHANOL EXTRACT OF
TUNJUK LANGIT (Helminthostachys
zaylanica) RHIZOME AS TABLET DOSAGE
FORM BY WET GRANULATION METHOD
PREPARATION AND CHARACTERIZATION OF
SUBMICRON PARTICLES OF PLGA
INCORPORATING RIFAMPIN USING
EMULSION SOLVENT DIFFUSION METHOD
LIQUID BATH SOAP FORMULATION AND
ANTIBACTERIAL ACTIVITY TEST AGAINST
Staphylococcus aureus OF KECOMBRANG
(Etlingera elatior (Jack) R.M.Sm.) FLOS
EXTRACTS
OPTIMIZING COMBINATION OF SAMBILOTO
HERBAL WATER FRACTION AND SALAM
LEAF WATER FRACTION AS ANTIINFLAMMATION
THE POTENTIAL OF THE BLACK RICE BRAN
EXTRACT AS ANTIDIABETIC AGENT
ANTI-INFLAMMATORY ACTIVITY TEST OF
CHRISTMAS PALM (Adonidia merrillii (Becc.)
Becc.) SEED EXTRACT IN MALE WISTAR
RATS (Rattus norvegicus)
PRE-CLINICAL STUDY OF CR (III) BASED
HYPOGLICEMIC SUPPLEMENT IN TYPE 2
DIABETIC RATS
SCREENING ANTIBACTERIAL POTENCY OF
METABOLITE ENDOPHYTIC FUNGI
MANGOSTEEN (Garcinia mangostana L.)
LEAF
ACTIVITIES OF THE COMBINED EXTRACTS
OF TEMPUYUNG (Sonchus arvensis) AND
BLACK CUMIN (Nigella sativa) AGAINST
XANTHINE OXIDASE INHIBITION ON
HYPERURICEMIC MICE
ANTIBACTERIAL ACTIVITY OF
COMBINATION OF CHLORAMPHENICOL AND
ETHANOLIC EXTRACT OF PACAR AIR
(Impatiens balsamina) LEAVES AGAINST
ANTIHYPERCHOLESTEROLEMIC EFFECT OF
MURBEI (Morus alba L.) LEAVES AND ITS
COMBINATION WITH SIMVASTATIN IN RATS
INDUCED BY PROPYLTIOURACIL AND HIGH
FAT DIET

No
13
Code
C001
Presenter
HENING
PRATIWI
Institution
UNSOED
14
C003
RASMAYA
NIRURI
UDAYANA
UNIVERSITY
15
D001
INDRI KUSUMA
DEWI
POLTEKKES
KEMENKES
SURAKARTA
16
D002
MUHAMMAD
ASWAD
TOYAMA
UNIVERSITY
17
E001
HARYOTO
UMS
18
E002
RETNO
ARIANINGRUM
UNY
19
E003
TRESNA
LESTARI
STIKES BAKTI
TUNAS
HUSADA

Title
OVERVIEW ABOUT ANTIBIOTICS
PRESCRIBING IN URINARY TRACT
INFECTION ROEMANI SEMARANG
HOSPITALS INPATIENTS
CLINICAL IMPROVEMENT AFTER
CEPHALOSPORINE THERAPY ON CHILDREN
WITH TYPHOID FEVER
ANTIOXIDANT ACTIVITY ETHYL ACETATE
EXTRACT OF SEED AND POD PARKIA (Parkia
speciosa Hassk.) WITH DPPH (1,1 DIPHENYL - 2 - PICRYLHYDRAZIL) METHOD
A CLICK-TYPE COUPLING REACTION
BETWEEN THIOAMIDES AND SULFONYL
AZIDES AS A VERSATILE APPROACH TO
GENERATE NEW PHARMACOLOGICALLY
ACTIVE COMPUNDS
CYTOTOXIC ACTIVITY OF POLAR,
SEMIPOLAR, AND NON POLAR FRACTION
ETHANOL EXTRACT OF LEAVES PLANTS
SALA (Cynometra ramiflora Linn.) AGAINTS
WiDr CELL
CHALCONES DERIVATIVE WITH BROMO
SUBSTITUENT INDUCES APOPTOSIS IN
HeLaA CELLS
SUB-CLONING OF ads GENE INTO
pETDUET1_cyp FOR CO-EXPRESSION IN
ESCHERICHIA COLI
xvii | P a g e
LIST OF POSTER PRESENTERS

No
1
Code
A002
Presenter
ANDI SRI SURIATI
AMAL
A004
ISKANDAR
ZULKARNAIN
A007
SHOLICHAH
ROHMANI
B002
DONI WIBOWO
B003
FEBBY LOVITA
SARI
UMS
B005
INTAN CAHYANI
STIFAR
YAYASAN
PHARMASI
B006
KEN LATIFA
UMS
B012
WESTI FAJRIN
BAYU
NUGRAHAINI
UMS
B013
SAPTO YULIANI
UAD
10
B014
FAJAR KHOLIKUL
AMRI
UMS
11
C002
IDA AYU PEBRINA
UMS
xviii | P a g e
Institution
UNIV.
DARUSSALA
M GONTOR
UNIV.
MUSLIM
INDONESIA
UNIV.
SEBELAS
MARET
UMS
Title
PREPARATION OF ARTIFICIAL SALIVA
FORMULATION
EFFECT COGRINDING ON THE PHYSICAL
CHARACTERISTIC OF BINARY MIXTURE OF
PIOGLITAZONE HCl AND METFORMIN HCL
THE INFLUENCE OF EGGPLANT PEEL
EXTRACT (Solanum melongena L.) AS
ANTIOXIDANT ON THE LOTION MATERIAL
ANTIOXIDANT ACTIVITIES OF
NANOEMULSION CONTAINING EXTRACT
OF SAMBILOTO (Andrographis paniculata
(Burm F.) Ness.) AND MENIRAN
(Phyllanthus niruri L.) IN ALLOXANINDUCED DIABETIC RATS
ACUTE TOXICITY STUDY OF
NANOEMULSION CONTAINING
SAMBILOTO (Andrographis paniculata)
LEAVES AND MENIRAN (Phyllanthus niruri)
HERBS EXTRACT IN WISTAR RATS
THE EFFECT OF GIVING GLUCOMANNAN
PORANG TUBER (Amorphophallus
oncophyllus Prain ex Hook. F.) ON SGPT
AND SGOT LEVELS OF WISTAR MALE RATS
BLOOD INDUCED BY PARACETAMOL
THE PROFILE OF MDA
(MALONDIALDEHYDE) LEVEL IN RATS
GIVEN EXTRACT OF THYMI HERBS
(Thymus vulgaris [L.])
EFFECTIVENESS TEST of 70% ETHANOL
EXTRACT ANALGETIK RED DRAGON FRUIT
PEEL (Hylocereus polyrhizus Cortex) WITH
STRETCHING METHOD ON MALE MICE
SWISS WEBSTER STRAIN
EFFECTS OF TURMERIC ETHANOLIC
EXTRACT ON TRIMETHYLTIN-INDUCED
OXIDATIVE STRESS ON SPRAGUEY
DAWLEY RATS
ANTIDIABETIC ACTIVITY OF
NANOEMULSION CONTAINING EXTRACT
OF SAMBILOTO (Andrographis paniculata
(BURM F.) NESS.) AND MENIRAN
(Phyllanthus niruri L.) IN ALLOXANINDUCED DIABETIC RATS
RATIONALITY TREATMENT OF
ANTIBIOTICS FOR TREATMENT OF
DIARRHEA IN ADULT PATIENTS IN THE
INSTALLATION INPATIENT HOSPITAL X
SURAKARTA 2014

No
12
Code
C004
Presenter
RASMAYA NIRURI
Institution
UDAYANA
UNIVERSITY
13
C005
SAKTYA AYU
PRACILLIA
UMS
14
D003
NANIK
SULISTYANI
UMS

Title
TRANSAMINITIS ASSOCIATED WITH HIGH
DOSE METHOTREXATE AND 6
MERCAPTOPURIN IN CHILDREN WITH
ACUTE LYMPHOBLASTIC LEUKEMIA
COST ANALYSIS AND DESCRIPTION
TREATMENT OF DIARRHEA IN PATIENTS
OF INPATIENT HOSPITAL dr. MOEWARDI
SURAKARTA BY BPJS PROGRAM IN 2014
ANTIBACTERIAL COMPOUND PRODUCED
BY A SOIL BACTERIA ISOLATED FROM
RIZHOSPHERE OF Zingiber officinale
xix | P a g e
SPEAKERS
NANOSTRUCTURED BIOMATERIALS FOR MEDICAL AND BIOLOGICAL
APPLICATIONS
Jackie Y. Ying
Institute of Bioengineering and Nanotechnology

31 Biopolis Way, The Nanos, Singapore 138669
E-mail: jyying@ibn.a-star.edu.sg
Nanostructured materials have been developed for various medical and biological applications.
They have been designed as stimuli-responsive drug delivery systems and sustained protein
delivery systems. Nanocomposite systems have also been derived to provide simultaneous drug
delivery and bioimaging functions as theranostic systems. Micellar nanocomplexes have been
synthesized with green tea-based ingredients as unique carrier materials that offer synergistic
therapeutic effects with the drugs to be delivered.
In addition, nanostructure processing has been employed in creating synthetic cell culture
substrates for the expansion and controlled differentiation of stem cells. Nanostructured
scaffolds have also been obtained for cell and tissue engineering.

1|P a g e
SPEAKERS
PHOTOAFFINITY LABELING-BASED TARGET IDENTIFICATION OF BIOACTIVE
MOLECULES
Takenori Tomohiro
Graduate School of Medicine and Pharmaceutical Sciences

University of Toyama
Email: ttomo@pha.u-toyama.ac.jp
Target identification and confirmation for small molecules is often the rate-limiting step in drug
discovery. Photoaffinity labeling (PAL) is a photochemical method to attach a tag with a
covalent bond into the ligand-interacting surface within target protein. Since PAL can access to
most of biological interacting systems including membrane protein or weak-binding protein
that are difficult to access by conventional affinity purification methods, it may be a powerful
method for identification of target protein that is also able to determine its interacting site.
However, conventional PAL-based identification method accompanied with isolation of the
target in very pure form often failed, especially in case of the target of a trace amount. The
purification process is often complicated by inevitable contamination that is due to non-specific
adsorption and significant loss during handling because of the different physical properties of
individual peptide fragments.
Recently, we designed a unique photoreactive unit that can install a high-performance chemical
tag, an isotope-coded fluorescent tag, to the interacting surface upon UV-irradiation. The
combination of PAL and heterogeneous target-selecting techniques significantly simplified the
procedure and reduced the amount of quantity and time required for identification. The details
will be presented.
2|P a g e

SPEAKERS
CURRENT STATUS OF RECOMBINANT THERAPEUTIC PROTEINS AND LOCAL
ISSUES RELATED
Wangsa Tirta Ismaya
Recombinant Therapeutic Proteins, Dexa Laboratories of Molecular Sciences, Dexa Medica

Industri Selatan V PP-7, Jababeka II Industrial Estate
Cikarang 17550, Indonesia
E-mail: wangsa.ismaya@dexa-medica.com
Generation of recombinant version of a therapeutic protein (rTP) has been long employed to
solve issues with availability of the protein from its natural resource. Such approach results in
production of the protein in large scale of controlled, engineered, and directed manner.
However, cost of both generation and production of the biological drug is high, thereby protein
theraphy is still not accessible for patients of limited income. Nevertheless, numerous rTPs have
been produced in the past decades and their demands keep on increasing.
Expiration of patents protecting current rTP allows production of its generic version called
biosimilar, which can be made available at lower prices from reduced costs of product
development and of pre-clinical and clinical trials. Production of biosimilar is done through
processes obtained from technology transfer or self-developed. Either way, validation of the
biosimilar product remains the major challenge. Biosimilar product must share identical
characteristics and molecular properties, and display similar safety and potency features to
those of originator. Alterations in the physico-chemical properties of the molecule or
improvement in potency of the drugs are not permitted.
On the other hands, development of new recombinant therapeutic protein continues as new
diseases or new variant of a disease emerge as well as reoccurrence of well-known diseases.
Developing a recombinant therapeutic protein is usually started with search of candidates
based on putative or known molecular basis of a disease. We are currently developing a new
approach by means of employing proteins of unknown function to screen for their potential use
in pharmaceutic or therapy. This approach involves various techniques in bioinformatics,
biochemistry, molecular biology and pharmacology, and cell physiology. This approach may
lead to discovery of novel pharmaceutical or therapeutic proteins. With the finding of many
genes that encode proteins of unknown function during elucidation of genomes (e.g. human),
this unusual approach provides alternative to discover new pharmaceutical/therapeutic
proteins. We may as well assign possible biological function of the proteins.
In Indonesia, development of biosimilar or recombinant therapeutic protein is still at infancy.
However, pharmaceutical industries embrace development and production of biosimilar,
especially to anticipate the coming Asean Economic Community implementation. The National
Agency for Food and Drugs Control (NAFDC) has been developing and preparing guidelines to
regulate and control the development and production of both biosimilar and rTP. Related to
Indonesian moslem population in particular, development and production of halal certified
drugs, including rTP and biosimilar are also part of the major issues. Especially after recently
the bill for halal consumer products has been passed. Thus, development of recombinant
therapeutic protein appears to gain its momentum in Indonesia in the near future.

3|P a g e
OP
A001
PHARMACEUTICAL TECHNOLOGY
CHARACTERISTICS TESTING OF MICROCRYSTALLINE CELLULOSE FROM NATA DE

COCO COMPARED TO AVICEL pH 101 AND AVICEL pH 102
Adi Yugatama1*, Laksmi Maharani2, Hening Pratiwi2, Lingga Ikaditya3
1Farmasi,
FMIPA, Universitas Sebelas Maret, Surakarta

FIKES, Universitas Jenderal Soedirman, Purwokerto
3Farmasi, Poltekkes Kemenkes Tasikmalaya, Tasikmalaya
2Farmasi,
*E-mail: adiyugatama.apt@gmail.com
Abstract
Microcrystalline cellulose is an imported raw material in Indonesia, which used widely
as an excipient in tablet production. One of the alternative materials to produce microcrystalline
cellulose is from nata de coco. This research aimed to know the characteristic of
microcrystalline cellulose from nata de coco compared to avicel pH 101 and avicel pH 102. Nata
de coco were alkalinated, dried and hydrolyzed to get microcrystalline cellulose. Independent
variables in this research are microcrystalline cellulose from nata de coco, avicel pH 101 and
avicel pH 102. While the dependent variables are flow properties, compactibility,
compressibility, water absorption, tap density, bulk density, loss of drying, infrared absorption
spectra, and SEM images. Data was analyzed using one way ANAVA with CI 95% and using
software SPSS for windows. The result showed that the characteristic of microcrystalline
cellulose from nata de coco is different in flow properties, compactibility, compressibility, tap
density, bulk density, and loss of drying from avicel pH 101 and avicel pH 102, but having the
same water absorption. Infrared spectrum data showed that microcrystalline cellulose from
nata de coco is similar to avicel pH 101 and avicel pH 102. The SEM result showed that
microcrystalline cellulose from nata de coco having bigger particle size (66.67266.67 m) than
avicel pH 101 (13.33166.67 m) and avicel pH 102 (13.33200 m).
Keywords: Avicel pH 101, Avicel pH 102, Nata de coco, microcrystalline cellulose.
4|P a g e

PP
A002
PREPARATION OF ARTIFICIAL SALIVA FORMULATION
Andi Sri Suriati Amal1*, Samsinah Hj. Hussain2, Mohd. Amin Jalaluddin3
1Universitas
Darussalam Gontor, Indonesia

Malaya, Malaysia
3Universiti Malaya, Malaysia
2Universiti
*E-mail: andiinci73@gmail.com
Abstract
Dry mouth or throat (xerostomia) is a clinical condition characterized by desiccation of
the intraoral tissues. Patients with chronic or temporary sensation of dry mouth need some
kind of treatment to relieve the symptoms. Causes of dry mouth include medications,
autoimmune disease (Sjogrens syndrome), radiotherapy or chemotherapy for cancer, hormone
disorders and infections. The project is important not only because saliva substitutes are not
manufactured locally, but also because most saliva substitutes use mucin (porcin in origin).
Therefore there is a need to produce one with other source which has properties to mucin itself.
The objective of this project is to produce saliva substitutes that can serve as mouth and throat
lubricants. The first step was pre-formulation studies that involved characterization of active
ingredients (physical, chemical, and mechanical properties) in order to choose what other
ingredients (excipients) should be used in the preparation. Formulation studies also considered
such factors as solubility, viscosity, and pH. The last step was assessment of safety and stability
of the final product.The new artificial saliva formulations containing various ratios of SCMC
(Sodium carboxymethyl cellulose), MC (methyl cellulose) and HPMC (hydroxypropyl
methycellulose) have been developed. Combination of cellulose derivatives and albumin in
these formulations resulted in the physical properties of these new artificial saliva substitutes
closely resembling human saliva and mucin-based saliva substitutes. Formula we choose were
the most suitable formulae due to their viscosity and pH properties which closely resemble
human saliva and mucin based saliva substitutes.
Keywords: artificial saliva, saliva substitute, mouth and throat lubricant, mouth and throat
moisturizer.

5|P a g e
OP
A003
FORMULATION OF ETHANOL EXTRACT OF TUNJUK LANGIT (Helminthostachys

zaylanica) RHIZOME AS TABLET DOSAGE FORM BY WET GRANULATION METHOD
Fitrya1*, Najma Annuria Fithri1, Budi Untari1, Aprililianti1
1Department
of Pharmacy, University of Sriwijaya

Indralaya, OI, South Sumatera, Indonesia
*E-mail: fitrya_apt@yahoo.com
Abstract
Tunjuk langit rhizome (Helminthostahcys zeylanica (Linn) Hook) has been used
traditionally as an anticancer and antiinflamatory agent. In addition, previous studies have
proven the potency of ethanol extract from the tunjuk langit rhizome (Helminthostahcys
zeylanica (Linn) Hook) as antihyperuricemic agent. In this study, ethanol extract of the rhizome
was formulated into a tablet dosage form by a wet granulation method. The tablet was prepared
with different types of disintegrant and binder, i.e. Formula A (starch: PVA), Formula B
(AvicelPH102: PVP), and Formula C (sodium alginate: methylcellulose). Physical properties
(such as weight variation, tablet diameter, thickness, friability, hardness, and disintegration
time) and dissolution of tablets were evaluated. The results showed that tablet A (starch: PVA)
produced the best physical properties and dissolution characteristics, which have met the
requirements. Therefore, the wet granulation method is suitable to develop the extract into
tablet.
Keywords: extract, tunjuk langit rhizome, Helminthostachys zaylanica, tablet, wet granulation.
6|P a g e

PP
A004
EFFECT COGRINDING ON THE PHYSICAL CHARACTERISTIC OF BINARY MIXTURE

OF PIOGLITAZONE HCl AND METFORMIN HCl
Iskandar Zulkarnain1*, S. N. Soewandhi1, S. Wikarsa2
1Fakultas
Farmasi Universitas Muslim Indonesia

Farmasi Institut Teknologi Bandung
2Sekolah
*E-mail: iskandarzulkarnain_03@yahoo.com
Abstract
This study was aimed to identify the physical interaction between PGZ-MFN by
cogrinding. Solid state interaction was observed by cold contact method and phase diagram
formation. The solid state grinding (SSG) and solvent drop grinding (SDG) was conducted on
binary mixtures. The identification resulted in a binary system was characterized by differential
thermal analysis (DTA), polarization microscopy, scanning electron microscope (SEM) and
powder X-ray diffraction (PXRD). Furthermore, the solubility and dissolution testing of PGZ
performed on the physical interaction results. The observation under the polarizing microscope
showed that the new crystal habit was not found. The mixture has melting point lower than
MFN and PGZ. This phenomenon was then confirmed with phase diagram arranged by
thermogram of PGZ-MFN. That was identified mixture binary equimolar as eutectic mixture
with eutectic temperature 187 C. Meanwhile, PXRD data at equimolar mixture did not showed
the new interference peak. The DTA and powder X-ray diffraction data of the equimolar solid
compounds obtained from several tehnique showed similar result. The thermogram of all
treated sampels had similar endothermic curve (185- 186C), and identical interference peaks
of PGZ-MFN of 2 8.6; 12.2; 12.8; 17.2; 18.6; 22.7; 23.2 . Conclusion Based on the results of
morphological analysis, the PXRD data and thermal properties, PGZ-MFN equimolar mixture
showed the formation of a eutectic mixture.
Keywords: Cogrinding, physical characteristic, binary system, pioglitazone HCl, metformin HCl.

7|P a g e
OP
A005
PREPARATION AND CHARACTERIZATION OF SUBMICRON PARTICLES OF PLGA

INCORPORATING RIFAMPIN USING EMULSION SOLVENT DIFFUSION METHOD
1Department
Mardiyanto1*
of Pharmacy, Faculty of Mathematics and Natural Science, University of Sriwijaya

Inderalaya, Indonesia
*E-mail: dianto72@yahoo.com
Abstract
The research had been performed to incorporate rifampin into PLGA submicron-sized
particles. This research has a prospect to be applied to overcome the ineffectiveness use of
rifampin for tuberculosis patients as rifampin was not stable in human lung macrophages, while
Mycobacterium tuberculosis was able to survive in human lung macrophages. Rifampin was
incorporated into submicron particles of PLGAs using the emulsion solvent diffusion method.
The use of rifampin 50 mg in every batch resulted in the submicron-sized particles of 220 nm,
PDI 0:12, zeta potential 21 mV and EE 37%. In the batch using rifampin 300 mg, resulted the
submicron-sized particles of 410 nm, PDI 0:22, zeta potential 14 mV and EE 40%. The surface of
the particles was visualized by SEM and hydrodynamic size compared to TEM. It was known
that particle is spherical with a smaller diameter than the hydrodynamic size. TEM
measurement revealed the size of particles with PVA was 208 nm.
Keywords: characterization, PLGA, rifampin, hydrodynamic-size, TEM, %EE.
8|P a g e

OP
A006
LIQUID BATH SOAP FORMULATION AND ANTIBACTERIAL ACTIVITY TEST

AGAINST Staphylococcus aureus OF KECOMBRANG
(Etlingera elatior (Jack) R.M.Sm.) FLOS EXTRACTS
1Pharmacy
Lilis Handrayani1, Ratih Aryani1*, Indra1
Study Programme, Sekolah Tinggi Ilmu Kesehatan Bakti Tunas Husada Tasikmalaya
*E-mail : ratih_aryani@ymail.com
Abstract
The formulation of kecombrang flos extract (Etlingera elatior (Jack) R.M.Sm.) liquid
bath soap has been established. The objective of this research was to formulate liquid bath soap
of kecombrang flos extract (Etlingera elatior (Jack) R.M.Sm.) and to test its antibacterial activity
to Staphylococcus aureus. Kecombrang flos extract was extracted by maceration method using
96% ethanol, and followed by minimum inhibitory concentration (MIC) test using hole method.
Concentration variation of kecombrang flos extract was conducted as F1 (6%), F2 (8%), and F3
(10%). The formula of liquid bath soap of kecombrang flos extract was evaluated using several
examinations such as organoleptic, pH, viscosity, density, foaming stability, antibacterial activity
test, irritation test and hedonic test. The result shows the liquid bath soap of kecombrang flos
extract F1, F2 and F3 can inhibit the growth of Staphylococcus aureus. Based on statistical test
using SPSS 21 (for trial) ANOVA method continued by LSD shows that F0 (negative control), F1,
F2, F3 and positive control (triclosan 2.5%) have difference meaningful result with significance
value < 0.05.
Keywords: Kecombrang flos extract, Etlingera elatior, liquid bath soap, antibacterial,
Staphylococcus aureus.

9|P a g e
PP
A007
THE INFLUENCE OF EGGPLANT PEEL EXTRACT (Solanum melongena L.) AS

ANTIOXIDANT IN LOTION DOSAGE FORM
Sholichah Rohmani1*
1Universitas
Sebelas Maret
Solo, Indonesia
*E-mail: licha.apt@gmail.com
Abstract
The purpose of this research is to formulate lotion containing extract of eggplant peel
as an effective and safe antioxidant lotion. Previous research showed that the anthocyanin in
peel eggplant has antioxidant activity against free radical that cause aging on the skin. Extract of
eggplant peel was obtained by maceration process using ethanol 70%. The extract was added in
various concentrations in lotion formulation i.e. 0%, 0.5%, 1%, 2% and 3% based on the
antioxidant activity that was determined using DPPH method. The produced lotions were
characterized for the antioxidant activity, stability and physical properties including
organoleptic, viscosity, pH and panellist acceptability. The result showed that antioxidant
activity rated weekly and decreasing viscosity from week 0 to 8, pH of 5.62-6.93 was observed.
Formulae I was the most acceptable lotion by pannelist.
Keywords: peel eggplant, lotion, antioxidant.
10 | P a g e

OP
A008
OPTIMIZING COMBINATION OF SAMBILOTO HERBAL WATER FRACTION AND

SALAM LEAF WATER FRACTION AS ANTI-INFLAMMATION
Raymond Harris Mustafa1*, Lannie Hadisoewignyo1, Martha Ervina1, Lisa
Soegianto1, Wahyu Dewi Tamayanti1
1Faculty
of Pharmacy, Widya Mandala Surabaya Catholic University

Surabaya, Indonesia
*E-mail: lanhadi@yahoo.com
Abstract
Sambiloto herbs (Andrographis paniculata Nees) and salam leaves (Syzygium

polyanthum), which are effective to reduce blood sugar level with different mechanism, have
been suggested to produce a synergy as antioxidant and anti-inflammatory agent, hence the
optimum combination formula is remained to be elaborated. By reducing blood sugar level and
showing antioxidant, and anti-inflammatory activity, both plants were hypothesized of its
ability for ameliorating diabetes mellitus complexicity. This study aimed to discover the optimal
combination formula of sambiloto herbs (Andrographis paniculata Nees) and salam leaves
(Syzygium polyanthum) water fraction to produce anti-inflammation effect. Carrageenan was
used to induced the inflammatory condition. Optimization was conducted by factorial design
utilising 2 factors and 2 levels. Both factors, sambiloto herbs (Andrographis paniculata Nees)
and salam leaves (Syzygium polyanthum) in the range of low level 1:10 and high level 10:1 with
the used doses were 100 mg/kg BW at low level and 300 mg/kg BW for high level. The observed
parameters were anti inflammation potential percent and edema rate. Conclusively, this study
proposed that optimum combination formula of sambiloto herbal : salam leaves water fraction =
1.14 : 9.87 with 234 mg dose. The combination formula was theoretically resulted the optimum
anti inflammation potential percent of 45.68%, and ER of 38.39% compared with other
combination formulas.
Keywords: salam leaf, sambiloto herbal, water fraction, anti inflammation, factorial design.

11 | P a g e
OP
B001
PHARMACOLOGY AND MICROBIOLOGY
THE POTENTIAL OF THE BLACK RICE BRAN EXTRACT AS ANTIDIABETIC AGENT

Arifah Sri Wahyuni1*, Rima Munawwaroh1, Esthi Utaminingsih1, Novita Sari1
1Faculty
of Pharmacy University Muhammadiyah Surakarta

*Email: arifah.wahyuni@ums.ac.id
Abstract
Natural hypoglycaemic compounds may be attractive alternatives to synthetic drugs or

reinforcements to currently used treatments. Black rice bran is one of natural compound that
containing anthocianins. The major anthocyanins component isolated from black rice bran is
cyanidin 3-glucoside. In this experiment, fiveteen male rat were randomly allocated into 5 equal
groups. They were induced by alloxan (150mg/kgbw, i.p) except Group I). If the blood glucose
levels reach 200 mg/dL, they were given aquadest (Group II), black rice bran extract (BBE)
respectively 50, 100 and 200 mg/kg.bw (Group III-V). Blood glucose level was analyzed at 4, 7,
10 days treatment. Insulin level was determined enzyme linked immunosorbent assay (ELISA).
The result showed that baseline levels of blood glucose were statistically not different among
the fivegroups (p>0.05). By the end of experiment BBE dose 50 mg/kgbw did not reduce blood
glucose levels after 10 days of treatment (p>0.05). BBE dose of 100 and 200 mg/kgbw
decreased blood glucose levels. The level of blood glucose after administered 10 days by BBE
200 mg/kgbw was 131.33 8.08 mg/dL. The results showed that insulin levels in diabetic rats
increased by administration of the extract at dose 200 mg/kg b.w was 15,209,5ng/mL. It can
be concluded that BBE can be therapeutically helpful as antidiabetic agents
Keywords: cyanidin 3-glucoside, black rice bran extract, antidiabetic.
12 | P a g e

PP
B002
ANTIOXIDANT ACTIVITIES OF NANOEMULSION CONTAINING EXTRACT OF

SAMBILOTO (Andrographis paniculata (Burm F.) Ness.) AND MENIRAN
(Phyllanthus niruriL.)IN ALLOXAN- INDUCED DIABETIC RATS
Erindyah R Wikantyasning1*, Nurcahyanti Wahyuningtyas1, Muhammad Dai1,
Doni Wibowo1
1
Faculty of Pharmacy, Universitas Muhammadiyah Surakarta

*E-mail : erindyah.rw@ums.ac.id
Abstract
Combination of an oral antidiabetic with antioxidant is potential to treat disease and

the complications of diabetes mellitus. The aim of this study was to investigate the antioxidant
activities of nanoemulsion containing extract of sambiloto (Andrographis paniculata (Burm F.)
Ness.) and meniran (Phyllanthus niruri, L.) (NESM) in alloxan-induced diabetic rats. Rats were
divided into 6 groups that were orally administered by nanoemulsion base (NE), vitamin E (100
mg/kg), NESM (100, 200 and 400 mg/kg) and combination of extract of sambiloto and meniran
(ESM) (400 mg/kg) for 14 days. Blood was withdrawn on day 0, 1, and 14, and malondialdehyde
(MDA) level was determined using UV-Vis spectrophotometer. The result showed that
increasing dose of NESM reduced plasma MDA levels compared to NE, vitamin E and ESM
groups. It can be concluded that NESM possess antioxidant activities.
Keywords: nanoemulsion, Andrographis paniculata, Phyllanthus niruri, malondialdehyde
(MDA), antioxidant.

13 | P a g e
PP
B003
ACUTE TOXICITY STUDY OF NANOEMULSION CONTAINING SAMBILOTO (Andrographis

paniculata) LEAVES AND MENIRAN (Phyllanthus niruri) HERBS EXTRACT
IN WISTAR RATS
Erindyah R. Wikantyasning1*, Nurcahyanti Wahyuningtyas1, Muhammad Dai1,

Febby Lovita Sari1
1Faculty
of Pharmacy, Universitas Muhammadiyah Surakarta

*Email: erindyah.rw@ums.ac.id
Abstract
Andrographis paniculata leaves (AL) and Phyllanthus niruri herbs (PH) have been
proven scientifically to have antidiabetic and antioxidant activities. Nanoemulsion system has
been developed containing of both AL and PH extracts, using Tween 80 and polyethylene glycol
as surfactant and cosurfactant, respectively. The present study is to investigate the acute
toxicity of the nanoemulsion on Wistar rats. Five groups of rats were orally treated with
nanoemulsion doses of 31.25, 125, 500, 2000 mg/kg and control. General behavior, adverse
effects and mortality were observed for up to 14 days. Oral administration of nanoemulsion at
the highest dose of 2000 mg/kg resulted in no mortalities or evidence of adverse effects,
indicated that nanoemulsion is nontoxic with a LD50 higher than 2000mg/kg. Throughout 14
days of the treatment, there was no significant change in behavior and body weight of rats in
both treatment and control groups. Histopalogical study showed that there were congestion in
the liver and inflammation in the kidney by the highest dose of 2000 mg/kg. In addition, the
pancreas and gastric of rats in the highest dose group showed a significant change compared to
the control group.
Keywords: acute toxicity, nanoemulsion, A. paniculata, P. niruri, herbal extract.
14 | P a g e

OP
B004
ANTI-INFLAMMATORY ACTIVITY TEST OF CHRISTMAS PALM (Adonidia merrillii

(Becc.) Becc.) SEED EXTRACT IN MALE WISTAR RATS (Rattus norvegicus)
Herlina1*, Fitrya1, Fithri Najma A1, Rahmawati Dwi Shafarina1
1Department
of Pharmacy, Faculty of Mathematics and Natural Sciences, Sriwijaya University

South Sumatera, Indonesia
*Email : rinaafdil@gmail.com
Abstract
Christmas palm (Adonidia merrillii (Becc.) Becc.) contains flavonoids, tannins,

triterpenoids, and steroids. Antioxidant activity of christmas palm has been investigated. Based
on research, antioxidant has a potential as an anti-inflammatory agent. Christmas palm seed
extract anti-inflammatory activity test with rat paw edema method which induced by
carragenaan has been done. Ethanolic, ethyl acetate, and n-hexane extract of christmas palm
were divided into three dose groups, 200 mg/kg BW, 400 mg/kg BW, and 800 mg/kg BW.
Negative control was CMC Na in distilled water and positive control was 0.82 mg/200 g BW
diclofenac Na. Thirty three Wistar male rats as test subjects were divided into 11 groups (1
negative control group, 1 positive control group, and 9 treatment groups). Anti-inflammatory
activity was tested by measuring the volume of rat paw edema using plethysmometer. The test
results showed the average of inhibition percentage of the ethanolic, ethyl acetate, and n-hexane
extracts were 46.05%; 29.95%; and 34.63% respectively. This shows that the ethanolic extract
has better inflammation inhibitory activity than ethyl acetate and n-hexane extracts. Nonparametric statistical Mann Whitney test of 800 mg/kg BW ethanolic extract at 30 and 60
showed that both were not significantly different (p>0.05) to diclofenac Na. Christmas palm
ethyl acetate extract has anti-inflammatory activity far below diclofenac Na, but its effect was
constant until 150. n-hexane extract of 800 mg/kg BW at 60 showed anti-inflammatory effect
that was almost equivalent to diclofenac Na (p>0.05). Christmas palm seed extract ED50 has
been calculated based on the average of edema inhibition percentage in rats. ED50 of christmas
palm ethanolic extract is 640.42 mg/kg BW.
Keywords: Adonidia merrillii (Becc.) Becc., anti-inflammatory, rat paw edema, diclofenac Na.

15 | P a g e
PP
B005
THE EFFECT OF GIVING GLUCOMANNAN PORANG TUBER (Amorphophallus

oncophyllus Prain ex Hook. F.) ON SGPT AND SGOT LEVELS OF MALE WISTAR RATS
BLOOD INDUCED BY PARACETAMOL
Intan Martha Cahyani1*, Bekti Nugraheni1
1Sekolah
Tinggi Ilmu Farmasi YAYASAN PHARMASI

Semarang, Indonesia
*E-mail: Intanmartha20@yahoo.co.id
Abstract
Indonesia is a country with a high prevalence of liver disease. Liver is important for
survival and plays a role in every metabolic function of the body. One of the causes of liver
disorder is because of drugs. One of the drugs that cause liver damage is paracetamol.
Glucomannan is a major component of the porang tuber that serves as a soluble fiber.
Glucomannan is thought to have hepatotherapy effects as a potential antioxidant. The aim of
this study is to determine the effect of glucomannan porang tuber (Amorphophallus oncophyllus
Prain ex Hook. F.) with graded doses on the blood levels of SGPT and SGOT wistar male rats
induced by paracetamol dose of 1,638 g/kg BB. This research was experimental study. The
treatment in this study was porang glucomannan with a dose of 25 mg / kg, 50 mg / kg and 100
mg / kg. The Population of the study was white male Wistar rats aged 3-4 months with a weight
of 180g - 250g, and healthy. The result of the analysis showed that glucomannan Porang
(Amorphophallus oncophyllus Prain ex Hook. F.) has an effect as hepatotherapy. Giving
glucomannan porang at a dose of 50 mg / kg rat has an effect to decrease blood levels of SGPT
and SGOT wistar male rats induced by paracetamol.
Keywords: Porang tuber (Amorphophallus oncophyllus Prain ex Hook. F.), SGPT, SGOT.
16 | P a g e

PP
B006
THE PROFILE OF MDA (malondialdehyde) LEVEL IN RATS GIVEN EXTRACT OF

THYMI HERBS (Thymus vulgaris [L.])
Ken Inayati Latifa1*, Tanti Azizah Sujono1, Ika T. Dian Kusumowati1
1Faculty

*E-mail: ken.inayati@gmail.com
Abstract
Oxidative stress occurs because of an imbalance between oxidants and antioxidants

which are then potentially cause damage to cells and reportedly play an important role in the
process of liver damage. Free radicals can increase lipid peroxidation, which will then be
decomposed into malondialdehyde (MDA) in the blood. MDA is a marker of cell damage caused
by free radicals. Extract of Thymi contains chemical compounds such as terpenoids, flavonoids,
aglycone, and phenolic acids. The content of flavonoid compounds contained in herbal Thymi
can serve as an antidote to the radical. The purpose of this study is to determine the effect of
herbal extracts Thymi in rats MDA levels. The study used 20 rats were divided into 4 groups.
Group I was a normal control group was given distilled water of 2.5 ml/kg, while group II-IV
were given Thymi herbal extract for 5 days with a dose of 50, 100, and 150 mg/KgBW,
respectively. Blood was drawn for MDA content measurement using spectrophotometer at a
wavelength of 530 nm. The results showed that herbal extracts Thymi can reduce levels of MDA
in groups II, III, and IV. Meanwhile, there was also a decrease of MDA level in grup 1. So in this
study decreased levels of MDA which occurs in group II, III, and IV can not be said as a result of
administration of herbal extracts Thymi due to a decrease in MDA levels also occurred in group
I were only given distilled water.
Keywords: Thymus vulgaris, MDA (malondialdehyde).

17 | P a g e
OP
B007
PRE-CLINICALSTUDY OF Cr (III) BASED HYPOGLICEMIC SUPPLEMENT IN-TYPE 2

DIABETIC RATS
1Faculty
Kun Sri Budiasih1*, Kartika Ratna Pertiwi1
of Mathematics and Natural Sciences, Yogyakarta State University

Yogyakarta, Indonesia
*E-mail: kunsb@uny.ac.id
kunsba@gmail.com
Abstract
The chromium (III)- amino acid complex based hypoglicemic agent was investigated
on nicotinamide-streptozotocin induced diabetic Wistar rats. The rats were divided into 7
groups each consist of 4 animals. Three groups are control (+) with chromium picolinate
(CrPic), control (-) diabetic group (DM), and control non diabetic (non DM). Furthermore, three
groups were examined on the effect of Cr-AA[Cr(-OH)(glu)(OH)2]26H2O at dose of (50, 150
and 300 g/day). In addition, the last group was studied on the effect of control group by
glibenclamide. The blood glucose levels were measured before and after treatment. The results
show that supplementation of Cr(III)-complex in 8 weeks decreased the blood glucose level in
the range of 46.446 79.593 %.
Keywords: hypoglicemic, chromium (III), amino acid, complexes, nicotinamide-streptozotocin,
diabetic rats.
18 | P a g e

OP
B008
SCREENING ANTIBACTERIAL POTENCY OF ENDOPHYTIC FUNGI METABOLITE OF

MANGOSTEEN (Garciniamangostana L.) LEAF
Lisa Soegianto1*, Martha Ervina1, Kevin Widjaja1, Angela Violita1
1Faculty
of Pharmacy, Widya Mandala Catholic University Surabaya

Surabaya, Indonesia
*E-mail: lisa.soegianto@yahoo.com
Abstract
Mangosteen (Garcinia mangostana L.) is a tropical plant which its fruit peel is widely
used as an antioxidant, anti-diarrhea, anti-inflammatory, antitumor, and as an antibacterial. The
previous study found the antibacterial activities of extract and metabolites of endophytic fungi
of mangosteen rind. This research was aimed to explore utilization of mangosteen leaf and to
screen antibacterial potency of the mangosteen leaf endophytic fungi metabolites. Endophytic
fungi were isolated from leaf of mangosteen in the Malt Extract Agar (MEA) medium in order to
get 2 colonies of endophytic fungi. Screening of potential antibacterial metabolites was assessed
using diffusion method and bioautography, obtained results of the antibacterial activity against
Escherichia coli (Ec) and Staphylococcus aureus (Sa) of the metabolites endophytic fungi of leaf.
Macroscopic and microscopic characteristics from fungi isolate which has antibacterial potency,
was observed and fermented into Potatoes Dextrose Yeast (PDY) medium for 14 days. At day
14, biomass and supernatant were separated and carried out separation by liquid-liquid
extraction. The supernatant and biomass were fractionated using n-hexane, ethyl acetate, and
water. Each fraction was eluated to several mobile phase and tested its antibacterial activity
against Ec and Sa. The result showed that there is a potential antibacterial activity of endophytic
fungi metabolites leaf ED2 against Sa. Bioautography result was observed that the compound
has antibacterial activity and is supposed as flavonoid compounds. It was supposed that
endophytic fungi ED2 was a group of Trichoderma.
Keywords: mangosteen (Garcinia mangostana L.), endophytic fungi, antibacterial activity,
Escherichia coli, Staphylococcus aureus.

19 | P a g e
OP
B009
ACTIVITIES OF THE COMBINED EXTRACTS OF TEMPUYUNG (Sonchus arvensis)

AND BLACK CUMIN (NIGELLA SATIVA) AGAINST XANTHINE OXIDASE INHIBITION
ON HYPERURICEMIC MICE
Muhtadi1*, Nurcahyanti Wahyuningtyas1, Andi Suhendi1, Septi Heryani1
1Faculty

*E-mail:
muhtadi@ums.ac.id
Abstract
The combination extracts of tempuyung (Sonchus arvensis) and black cumin (Nigella
sativa) can decrease uric acid levels on the previous result research. However, the mechanism of
decreasing uric acid levels was unknown certainly. This study aimed to determine the inhibitory
effect of xanthine oxidase by the combination extracts of black cumin and tempuyung on
hyperuricemic mice. Hyperuricemic mice were induced by 250 mg/kgBW potassium oxonate
was given one hour before treatment. The mice were divided into 3 groups, group I was
given 10 mg/kgBW allopurinol (positive control), group II was given 0.5 mL/20gBW aquadest
(negative control) and group III was given the combination extracts of black cumin-tempuyung
with dose 200 mg/kgBW during 4 days administration. The supernatant of liver was taken and
measured of xanthine oxidase levels by spectrophotometer UV at 290 nm. The data of xanthine
oxidase activity were analyzed by Kruskal-Wallis and Mann-Whitney method. Xanthine oxidase
activity of the combination extracts of blackcumin-tempuyung was 4.540.9 U/mg, very
significantly than control negative was 8.000.22 U/mg (p<0.05). Inhibition of xanthine oxidase
by the combination extracts of black cumin-tempuyung was 43.2611.29%, lower than
allopurinol was 90.190.36%.
Keywords: Hyperuricemic, xanthine oxidase inhibition, Sonchus arvensis, Nigella sativa.
20 | P a g e

OP
B010
ANTIBACTERIAL ACTIVITY OF COMBINATION OF CHLORAMPHENICOL AND

ETHANOLIC EXTRACT OF PACAR AIR (Impatiens balsamina) LEAVES AGAINST
Ratna Yuliani1*, Agung Cokro Prabowo1, Yeni Maisyah1
1Faculty

*E-mail: ry149@ums.ac.id
Abstract
Chloramphenicol, a broad spectrum antibiotic, is used to treat several bacterial

infections. However, it has several side effects such as headache, rash, diarrhea, nausea,
vomiting, bone marrow suppression, and aplastic anemia. Combining antibiotic with another
antibacterial agent may decrease the dose thus the side effects. This study aimed to investigate
the antibacterial activity of chloramphenicol combined with ethanolic extract of pacar air
(Impatiens balsamina) leaves against Escherichia coli and Shigella sonnei. Chloramphenicol (30
g), extract (2500 g), combination of chloramphenicol and extract (22.5 g + 625 g and 15 g
+ 1250 g), dimethylsulfoxide, and water for injection were tested for antibacterial activity
against Escherichia coli and Shigella sonnei using well diffusion method. Data was analyzed using
Mann-Whitney test.The results showed that only chloramphenicol (22.5 g) combined with
extract (625 g) has inhibition zone diameter, which was not significantly different from
chloramphenicol alone (30 g) when it tested against E. coli. This result indicated that
combination of the antibiotic in lower concentration and extract can achieve the same
antibacterial activity as antibiotic alone in higher concentration.
Keywords: antibacterial, chloramphenicol, Impatiens balsamina, combination.

21 | P a g e
OP
B011
ANTIHYPERCHOLESTEROLEMIC EFFECT OF Murbei (Morus alba L.) LEAVES AND

ITS COMBINATION WITH SIMVASTATIN IN RATS INDUCED BY PROPYLTIOURACIL
AND HIGH FAT DIET
Tanti Azizah Sujono1*, Haryoto1, Ratna Kartikasari1, Laily Ieda Quntari1
1Faculty

*E-mail:tanti.azizah@ums.ac.id
Abstract
Empirically Murbei leaves are used to treat diseases such as hypercholesterolemia.

Murbei leaves contain quercetin 3- (6-malonylglucoside) and rutin which shows a strong
inhibitory effect on LDL oxidation. This study aimed to verify the effect of the ethanol extract of
murbei leaves in lowering total cholesterol in hyperlipidemic rats and the effect of combination
extract murbei with simvastatin to the hypocholesterolemic effect of simvastatin. Twenty four
Wistar male rats were divided into 6 groups randomly, group I-VI were given high fat diet and
(propyltiouracil) PTU to induce hypercholesterolemia. Group I as positive control was treated
simvastatin 3.6 mg/kgbw, group II as a negative control was given CMC Na, group III-V were
given ethanol extract of murbei leaves orally at a dose of 0.4; 0.6; and 0.8 g/kgbw respectively,
group VI were treated a combination of murbei extract 0.4 g/kg with simvastatin. Total
cholesterol was measured using a spectrophotometer visible ( = 500 nm) with CHOD-PAP
method as many as 3 times, i.e on day 0 (baseline), 14 days after induced hypercholesterolemia
with PTU and high-fat-diet and 14 days after treatment of murbei extract with still be given PTU
and a high-fat diet. The results showed that murbei leaves extract dose 0.4; 0.6 and 0.8 g/kg
were able to reduce total cholesterol with percent decrease in cholesterol levels by 32.94
10.07; 40.17 4.61 and 45.71 4.27% respectively. Ethanol extract of murbei 0.4 g/kg can
increase hypocholesterolemic effect of simvastatin when used in combination (p< 0.05).
Keywords : Murbei (Morus alba L), antihypercholesterolemic, propyltiouracil, high fat diet.
22 | P a g e

PP
B012
ANALGESIC EFFECT OF ETHANOLIC EXTRACT RED DRAGON FRUIT ( Hylocereus

polyrhizus Cortex) PEEL ON MALE MICE SWISS WEBSTER WITH
WRITHING REFLEX METHOD
Westi Fajrin Bayu Nugrahaini1*, Tanti Azizah Sujono1
1Faculty

*E-mail:westifbayun@gmail.com
Abstract
Red Dragon fruit (Hylocereus polyrhizus) is one of the plants that grow in Indonesia.
Peel of fruit (Hylocereus polyrhizus) contain oligosacharida and Betasianin. Oligosacharida
showed activity as prebiotic, which can lower the resistance to acidic conditions in the stomach,
meanwhile betasianin can protect the cells from damage caused by free radical. This research
aims to prove whether peel of Dragon fruit has activity as analgesic in mice. Twenty five mice
were divided into 5 groups. group I was given Na CMC 1% (negative control), group II was
treated paracetamol 65 mg/kgBW (positive control), group III, IV, and V were given ethanolic
extracts of dragon fruit peel at dose 0.25, 0.5, and 1 g/kgBW respectively. Twenty minutes later,
all mice were induced pain by 0,1 ml intraperitoneal acetic acid 1% and the cumulative of
writhing reflect of mice were calculated for one hour. Then percent inhibition of writhing were
analyzed by Kruskal Wallis test and continued by Mann-Whitney with 95% confidence level.
The results showed that ethanolic extracts of dragon fruit peel showed analgesic effect. It can
decrease the number abdominal constrictions and also increased the percentage inhibition of
writhing at dose of 0.25, 0.5, and 1 g/kgBW with percent of inhibition 42,76 2,04; 1.42
49,32; and 61,38 1,37% respectively.
Keywords: Hylocereus polyrhizus, Dragon fruit peel, analgesic, writhing reflect

23 | P a g e
PP
B013
EFFECTS OF TURMERIC ETHANOLIC EXTRACT ON TRIMETHYLTIN-INDUCED

OXIDATIVE STRESS ON SPRAGUEY DAWLEY RATS
Sapto Yuliani1*, Mustofa Mustofa2, Ginus Partadiredja2
1Faculty
of Pharmacy, Universitas Ahmad Dahlan

of Medicine, Universitas Gadjah Mada
2Faculty
*E-mail: syuliani@yahoo.com
Abstract
Oxidative stress, an imbalance between free radicals and the antioxidant system, is
known to contribute to the pathogenesis of the development of dementia. Ethanolic extract from
turmeric (Curcuma longa L.) containing the curcumin constituent has been reported to produce
antioxidant effects. This study aims to determine the effect of turmeric extract on markers of
oxidative stress in Spraguey Dawley rat induced by (trimethyltin) TMT. Thirty six adult male
Sprague-Dawley rats (195-215 g) were divided randomly into six groups consisting of 6 rats for
each group. The rats were divided randomly into six groups, i.e.: N group, which served as a
normal control group; T group, which was given intra-peritoneal injection of TMT chloride; TCit group, which was treated with oral citicoline and TMT chloride injection; and three T-TE
groups, which were treated with three different dosages of oral turmeric rhizome extract, as
well as TMT chloride.The turmeric rhizome extract and citicoline solutions were given at day 1
up to day 28 of experiment, whereas the TMT chloride injection given as a single dose of 8 mg
/kg bw was administered at day 8 of experiment. At day 36 the blood were taken for plasma
MDA level determination. Afterthat all rats were sacrificed and the cerebral hemisphere were
then dissected out from the skull. The left cerebral hemispheres were used for biochemical
observation i.e. MDA and GSH level, activities of SOD, catalase (CAT) and GPx. The turmeric
extract dose of 200 mg/kg bw could prevent oxidative stress induced by TMT through the
decrease of levels of plasma and brain MDA and increased the activities of SOD, CAT, GPx, and
the level of GSH of brain. These effects seem to be comparable to those of citicoline.
Keywords: Curcuma longa L., trimethyltin, oxidative stress.
24 | P a g e

PP
B014
ANTIDIABETIC ACTIVITY OF NANOEMULSION CONTAINING EXTRACT OF

SAMBILOTO (Andrographis paniculata (BURM F.) NESS.) AND MENIRAN
(Phyllanthus niruri L.) IN ALLOXAN-INDUCED DIABETIC RATS
Erindyah R. Wikantyasning1*, Nurcahyanti Wahyuningtyas1, Muhammad Dai1,
Fajar Kholikul Amri1
1Faculty

*E-mail : erindyah.rw@ums.ac.id
Abstract
Preparation of nanoemulsion containing combination of extract of A. Paniculataand P.

Niruri (NESM) has been successfully developed. The present study was carried out to investigate
the antidiabetic activity of the nanoemulsion preparation in alloxan-induced diabetic rats.Rats
were divided into 6 groups that were given oral administration of nanoemulsion base (NE),
glibenclamide (5 mg/kg), NESM (100, 200 and 400 mg/kg) and combination of extract of
sambiloto and meniran (ESM) (400 mg/kg) for 14 days. The hypoglycemic effect was measured
by blood glucose level. Oral administration of the NESM at a dose of 100, 200, and 400 mg/kg
daily for 14 days showed a significant (P<0.05) decrease in blood glucose level as compared
with the diabetic rats. The results indicated that the nanoemulsion preparation possesses
significant antidiabetic activity.
Keywords: nanoemulsion, Andrographis paniculata (Burm F.) Ness., Phyllanthus niruri, L.,
antidiabetic.

25 | P a g e
OP
C001
CLINICAL AND COMMUNITY PHARMACY
OVERVIEW ABOUT ANTIBIOTICS PRESCRIBING IN URINARY TRACT INFECTION

ROEMANI SEMARANG HOSPITALS INPATIENTS
1Studied
Hening Pratiwi1*
Program of Pharmacy UNSOED

Purwokerto, Indonesia
*E-mail: hening170688@gmail.com
Abstract
Inappropriate antibiotics prescribing in Urinary Tract Infection (UTI) can lead antibiotics
resistance. Therefore, hospitals should have a formulary as a reference for providing medical
services to the patients. This study aims to determine the types of antibiotics that prescribed for
UTI treatment on January until November 2009 and determine the level of antibiotics
prescribing conformity with the Roemani Semarang hospitals formulary and WHO 2001
guidelines. This study used a non-analytical descriptive design and retrospectively. The samples
were 73 patients. This study includes the pattern of antibiotic prescribing in UTI patients and
conformity with 2009 hospital formulary and 2001 WHO guidelines. The results showed that
antibiotics are widely used cefotaxime (cephalosporins) 14 cases (24%), levofloxacin
(quinolones) 11 cases (18%), and ceftriaxone (cephalosporins) 10 cases (17%). The
combination that widely prescribed are cephalosporins combination with quinolones 3 cases
(21%), cephalosporin combination with other cephalosporins 3 cases (21%), combination of
cephalosporin with an aminoglycoside 2 cases (14%), there are 68 prescriptions (93%) suitable
with hospital formulary, and 5 prescriptions (7%) not listed on the formulary of Roemani
hospital 2009. The UTI antibiotic monotherapy in women, men, and children do not exist in
accordance with the WHO guidelines 2001.
Keywords: Urinary Tract Infections, antibiotics, hospital formulary, RS. Roemani Semarang.
26 | P a g e

PP
C002
RATIONALITY TREATMENT OF ANTIBIOTICS FOR TREATMENT OF DIARRHEA IN

ADULT PATIENTS IN THE INPATIENT INSTALLATION OF HOSPITAL X
SURAKARTA IN 2014
Ida Ayu Pebrina1*, Suharsono1, Suprapto1
1Faculty

*E-mail: deayu15@gmail.com
Abstract
Diarrhea is still a public health problem in developing countries such as Indonesia, due
to its morbidity and mortality are still high. Antibiotics are used to attack microbial infection
and kill or inhibit the growth of bacteria. The purpose of this study is to determine the
rationality of the use of antibiotics in adult patients with diarrhea in the inpatient installation of
hospital "X" Surakarta in 2014. The study used non-experimental method. The study used 46
medical records of patients using purposive sampling technique. The instrument that used is the
data collection sheets and the medical records of diarrhea patient as the material. From the data
that has been captured and processed, the rationality of antibiotic therapy for the treatment of
diarrhea include: precise indication of the percentage of 100 % , right drug as much as 97.82%,
right dosage as much as 97,82% , proper frequency and duration of drug administration as
much as 78.26%.
Keywords: antibiotics, diarrhea, adult patient.

27 | P a g e
OP
C003
CLINICAL IMPROVEMENT AFTER CEPHALOSPORINE THERAPY

ON CHILDREN WITH TYPHOID FEVER
Rasmaya Niruri1*, N.P. Yulia Purnami1, Julius D.Tansale2,3, I.B.Eka Erlangga3
1Department
of Pharmacy, Faculty Math and Science, Udayana University

2Petri Bali, Bali
3Puri Raharja Hospital, Denpasar, Bali, Indonesia
*E-mail: rasmaya@yahoo.com
Abstract
The aim of this research is to evaluate clinical recovery time on children with typhoid
fever (TF) after receiving cephalosporin. This cross-sectional research was conducted in Puri
Raharja Hospital. Data of 12 TF clinical features was collected from all pediatric patients with TF
on the period of January 1st, 2013 to March 31st, 2014, who received the same antibacterial
medicine during hospitalization. A patient, who had fever clearance and showed general wellbeing, was considered having a good clinical-response from TF. A patient was discharged after
showing a good clinical therapeutic response. Seventy nine from 89 subjects were prescribed
with cefotaxime, and the rest got ceftriaxone (9 patients) and cefixime (1 child). Length of stay
in the hospital (median [time range] in days) were 5 [4-10] with cefotaxime; 4 [4-7] with
ceftriaxone; and 7 [7] with cefixime. All of 89 children showed good clinical-response to the
medicines. The majority of children were free from TF symptoms when they were discharged,
but not from weakness- fatigue.
Keywords: clinical improvement, children, typhoid.
28 | P a g e

PP
C004
TRANSAMINITIS ASSOCIATED WITH HIGH DOSE METHOTREXATE AND

6-MERCAPTOPURIN IN CHILDREN WITH ACUTE LYMPHOBLASTIC LEUKEMIA
Rasmaya Niruri1*, K. Trisna Komalasari1, Ketut Ariawati2
1Department
2, Division
of Pharmacy, Faculty of Math and Science, Udayana University, Bali

of Hematology-Oncology, Department of Pediatric, Sanglah Hospital, Bali
Bali, Indonesia
*E-mail: rasmaya@yahoo.com
Abstract
The objective of this research was to evaluate the level of AST and AST before and after
consolidation phase of Indonesian Protocol (2006) Chemotherapy on acute lymphoblastic
leukemia (ALL).This cross sectional research was conducted in Sanglah Hopital. All hospitalized
children (0-12 tahun) in the period of January 2012 May 2014, who got high dose
methotreaxate (HD MTX) and 6-mercaptopurin (6-MP) and had normal AST-ALT level before
receiving HD -MTX- and 6-MP, were included. Thirteen of 39 patients who met the criteria was
obtained, but only ten patients, who had complete AST-ALT data on week 8th, 10th, and 12th of
consolidation phase. From 10 children who had the complete data, four of them (who didnt
receive Ursodeoxycholic acid (UDCA)) had the highest transaminase enzyme level at week 12th
and the rest 6 patients showed declining AST-ALT level after receiving UDCA.
Keywords: transaminitis, HD-MTX, 6-MP, children, ALL.

29 | P a g e
PP
C005
TREATMENT AND COST ANALYSIS OF DIARRHEA PATIENTS OF INPATIENT

INSTALLATION OF RSUD dr. MOEWARDI SURAKARTA BY BPJS PROGRAM IN 2014
Saktya Ayu Donna P1*, Suharsono1, Suprapto1
1Faculty

*E-mail: pracilliad@yahoo.com
Abstract
Diarrhea or gastroenteritis is still a major disease in developing countries that can lead
to death. This research was aimed to analyse the cost and treatment of gastroenteritiss patients
of inpatient installation using BPJS program at RSUD Dr. Moewardi in 2014. This research used
descriptive, non-experimental, and retrospective method by collecting data from the medical
report of patients. There are 28 cases fulfil inclusive criterion. Cost was analysed including
average total cost, prescription, medical stay, laboratory, visit, action and health tools and
administration cost. The result showed that (a) Average total cost of diarrhea therapy every
class treatment: VIP class is Rp.3.239.007 Rp.1.914.830, class I is Rp.1.522.475 Rp.0, class II
is Rp.2.964.331 Rp.368.155; class III Rp.2.043.954 Rp.1.716.563. (b) class treatment with
most cheap is class III is Rp.2.043.954 Rp.1.716.563. Stay care diarrheas patient of BPJS at
RSUD Dr. Moewardi year 2014 uses medicine groups are rehydration liquid (100%) RL is 17
cases (65.38%) and asering is 9 cases (34.62%). Antibiotic groups are 81 patients (81%),
adsorben group is 75 cases (75.0%), antiulcer kidney group is 16 cases (19.75%), antiemetic is
9 cases (11.11%), analgetic-antipeuritic 9 cases (11.11%) supplement food is 9 cases (11.11%)
and probiotic is 1 case (1.23%).
Keywords: cost analysis, RSUD dr. Moewardi, diarrhea.
30 | P a g e

OP
D001
PHARMACEUTICAL CHEMISTRY
ANTIOXIDANT ACTIVITY OF ETHYL ACETATE EXTRACT OF PARKIA SEED AND POD

(Parkia speciosa Hassk.) BY DPPH (1,1-DIPHENYL-2- PICRYLHYDRAZYL) METHOD
Indri Kusuma Dewi1*, Agus Winarso1
1Department
of Jamu Poltekkes Kemenkes Surakarta

Klaten, Indonesia
*E-mail: indri.kusumadewi@gmail.com
Abstract
Parkia speciosa is one of plants that usually used for food materials. Seed and pod of
Parkia speciosa contain flavonoid that act as antioxidant. The aim of this study was to examine
antioxidant activity at ethyl acetate extract of seed and pod Parkia speciosa tha were expressed
by Inhibitor Concentration 50 (IC50). Testing of antioxidant activity was done by DPPH method
(1,1-dipheny-2-picrylhydrazyl) using spectrophotometer UV-Vis at wavelength 518 nm. The
result showed that ethyl acetate extract of the seed of Parkia speciosa had low antioxidant
activity with the IC50 value of 274.702 ppm, while ethyl acetate extract of the pod Parkia
speciosa did not show any antioxidant effect with the IC50 value of 685.857 ppm.
Keywords: Parkia speciosa, seed, pod, antioxidant, DPPH.

31 | P a g e
OP
D002
A CLICK-TYPE COUPLING REACTION BETWEEN THIOAMIDES AND SULFONYL

AZIDES AS A VERSATILE APPROACH TO GENERATE NEW PHARMACOLOGICALLY
ACTIVE COMPOUNDS
Muhammad Aswad1, Junya Chiba1*, Yasumaru Hatanaka1, Takenori Tomohiro1
1Graduate
School of Medicine and Pharmaceutical Sciences, University of Toyama

Sugitani, Toyama, Japan
*E-mail: chiba@pha.u-toyama.ac.jp
Abstract
Several click-type reactions have been developed and applied to biological conditions,
however, the ligations generally need some catalysts or additives for their practical use. We
recently reported a chemoselective reaction between thioamides and sulfonyl azides to afford
sulfonyl amidines in the absence of any activation additives.1 The reaction proceeded by mixing
the thioamide and sulfonyl azide at room temperature in various solvents, and water exhibited
the highest performance with respect to efficiency. The characteristics of amidines within the
product framework are polar and hydrophilic, so that we applied this reaction for the simple
derivatization of sugars, such as nojirimycin, to develop novel inhibitors for glucosidases. A
cyclic thioamide derivative of nojirimycin could be synthesized from gluconolactone
derivatives.2By coupling of the thioamide with several sulfonyl azides, the chemoselective
ligation successfully afforded the corresponding amidine compounds, which are the potential
candidates as novel glycosidase inhibitors.
32 | P a g e

PP
D003
ANTIBACTERIAL COMPOUND PRODUCED BY A SOIL BACTERIA ISOLATED FROM

RIZHOSPHERE OF Zingiber officinale
Nanik Sulistyani1,2*, Yosi Bayu Murti3, Jaka Widada4, Mustofa5
1Faculty
of Pharmacy, Universitas Ahmad Dahlan

Program, Faculty of Medicine, Universitas Gadjah Mada
3Faculty of Pharmacy, Universitas Gadjah Mada
4Departement of Microbiology, Faculty of Agriculture, Universitas Gadjah Mada
5Departement of Pharmacology and Therapy, Faculty of Medicine, Universitas Gadjah Mada
2Doctoral
*E-mail: naniksulistyani@gmail.com
Abstract
Isolation of bacteria from Zingiber officinale rizosphere in Magelang, Central Java,
Indonesia has been carried out and got many isolates in the previous study. One of them is
isolate J4 having potencial activity as antibiotic producer. This study aims to identify the
antibiotic produced by isolate J4. Research was performed with examining the antibacterial
activity of ethyl acetate extracts of culture broth and their fractions using cup-plate method as
well as bioautography. The active fractions were then analysed using IR Spectroscopy.
Identification of selected bacteria was based on the 16S rRNA gene sequence and the active
fraction was characterized by LC-TOF-MS to identify the molecular mass of compounds
contained in the fractions. Result showed the isolate J4 revealed antibacterial activity of both
extracts and their fractions. The active fraction is the chloroform-methanol (7:3) fraction.
According to its IR spectra, there was detected OH and CH stretching vibration as well as
carbonyl stretching. Based on the LC-TOF-MS, the active fraction contains 3 compounds with
molecular mass of 270, 274 and 404. The BLAST of 16S rRNA sequence revealed that isolate J4
is Burkholderia sp.
Keywords: antibiotic, isolate J4, soil bacteria, Burkholderia sp.

33 | P a g e
OP
E001
MOLECULAR BIOLOGY
CYTOTOXIC ACTIVITY OF POLAR, SEMIPOLAR, AND NON POLAR FRACTION OF

ETHANOL EXTRACT OF SALA PLANTS LEAVES (Cynometra ramiflora Linn.)
AGAINTS WiDr CELL
Haryoto1*, Anis N. Irjayanti1, Tanti Azizah Sujono1, Muhtadi1, Andi Suhendi1
1Faculty

*E-mail: haryoto@ums.ac.id
Abstract
Previous study reported that ethanolic extract of Sala plants leaves (Cynometra
ramiflora Linn.) had cytotoxic activity against WiDr cells with IC50 of 6.37 g/mL. This study
aims to determine the cytotoxic activity of polar, semipolar, and nonpolar fraction of ethanolic
extract of Sala plants leaves (Cynometra ramiflora Linn.) against WiDr cells, their IC50s, and
compounds contained in each fraction of ethanol extract of Sala plants leaves. Fractionation
was performed using Vacuum Liquid Chromatography method with silica G60 as stationary
phase and n-hexane:ethylacetate (8:2, 7,5:2,5, 7:3,6:4, and 3:7) and ethanol as mobile phase.
Compounds contained in each fraction was analysed using thin layer chromatography method
with silica GF254 as stationary phase and mobile phase n-hexane:ethylacetate (7:3). Cytotoxic
activity assay was performed using MTT assay method. Polar fraction of ethanol extract of Sala
plants leaves has cytotoxic activity against WiDr cells with IC50 value of 231.953 g/mL.
Semipolar and nonpolar fractions do not show cytotoxic activity against WiDr cells.
Doxorubicin was used as positive control and obtained IC50 of 1.721 g/mL. The polar
fraction contains of flavonoids, phenolics and alkaloids, while the non-polar and semipolar
fractions contain phenolic compounds and alkaloids.
Keywords: Cytotoxic, fractionation, MTT assay, Cynometra ramiflora, WiDrcell, IC50.
34 | P a g e

OP
E002
MOLECULAR BIOLOGY
CHALCONES DERIVATIVE WITH BROMO SUBSTITUENT INDUCES APOPTOSIS IN

HeLa CELLS
Retno Arianingrum1*, Indyah Sulistyo Arty1
1Department
of Chemistry Education, Universitas Negeri Yogyakarta

*E-mail : Arianingrum_uny@yahoo.com
Arianingrum@uny.ac.id
Abstract
Chalcones, a group of aromatic enones, is known have a variety of biological

properties, including anticancer. We have synthesized a chalcone derivate 1-(4-bromophenyl) 3-(4-hydroxy-3-methoxyphenyl)-2-propene-1-on by aldol condensation reaction. The aim of the
present study was to investigate the anticancer potency of the compound in HeLa cervical
cancer cells by observing cytotoxic and apoptotic effect. The cytotoxic properties were
determined using MTT assay and apoptosis induction was carried out using flowcytometry. The
result indicated that the compound has cytotoxic effect on HeLa cells with IC50 of 53 M and was
able to induce apoptosis. Hence, 1-(4-bromophenyl) -3-(4-hydroxy-3-methoxyphenyl)-2propen-1-on is potential to be developed as anticancer agent for cervix cancer by inducing
apoptosis. However, the molecular mechanisms need to be explored further.
Keywords: chalcones derivate with Bromo substituens, cytotoxic, apoptosis, HeLa.

35 | P a g e
OP
E003
MOLECULAR BIOLOGY
SUB-CLONING OF ads GENE INTO pETDUET1_cyp FOR CO-EXPRESSION

IN ESCHERICHIA COLI
Imam A. Wicaksono1, Tresna Lestari2*, Evi U. Ulfa3, Catur Riyani1, Elfahmi1
1Institut
2STIKes
Teknologi Bandung
Bakti Tunas Husada Tasikmalaya
3Universitas Jember
Indonesia
*E-mail: beatsign@yahoo.com
Abstract
CYP71AV1 and ADS are two enzymes involved in artemisinin biosynthesis. In this
research, sub-cloning of cyp71av1 and ads in pETDUET1 (pETDUET1_cyp/ads) has been done.
The result of transformation has been confirmed by migration, restriction and sequencing
analysis. Overproduction of CYP71AV1 and ADS was done at temperature 37 C using 0.5 mM
IPTG induction. The protein produced mostly formed as inclusion bodies, therefore the
optimization of overproduction condition is still needed.
Keywords: CYP71AV1, ADS, pETDUET1, Sub-cloning.
36 | P a g e

CV OF SPEAKER
Jackie Y. Ying received her B.E. and Ph.D. from The Cooper Union and
Princeton University, respectively. She joined the faculty at
Massachusetts Institute of Technology in 1992, where she was
Professor of Chemical Engineering until 2005. She has been the
Founding Executive Director of the Institute of Bioengineering and
Nanotechnology in Singapore since 2003. For her research on
nanostructured materials, Prof. Ying has been recognized with the
American Ceramic Society Ross C. Purdy Award, David and Lucile
Packard Fellowship, Office of Naval Research Young Investigator Award,
National Science Foundation Young Investigator Award, Camille Dreyfus
Teacher-Scholar Award, American Chemical Society Faculty Fellowship
Award in Solid-State Chemistry, Technology Reviews Inaugural TR100 Young Innovator Award,
American Institute of Chemical Engineers (AIChE) Allan P. Colburn Award, Singapore National
Institute of Chemistry-BASF Award in Materials Chemistry, Wall Street Journal Asias Asian
Innovation Silver Award, International Union of Biochemistry and Molecular Biology Jubilee
Medal, Materials Research Society Fellowship, Royal Society of Chemistry Fellowship, American
Institute for Medical and Biological Engineering Fellowship, and Crown Prince Grand Prize in
the Brunei Creative, Innovative Product and Technological Advancement (CIPTA) Award.
Prof. Ying was elected a World Economic Forum Young Global Leader, and a member of the
German National Academy of Sciences, Leopoldina. She was named one of the One Hundred
Engineers of the Modern Era by AIChE in its Centennial Celebration. She was selected by The
Muslim 500 in 2012, 2013 and 2014 to be one of the worlds 500 most influential muslims. She
was selected as an Inaugural Inductee for the Singapore Womens Hall of Fame in 2014. She is
the Editor-in-Chief of Nano Today, which has an impact factor of 15.000.

37 | P a g e
CV OF SPEAKER
Full name: Takenori Tomohiro, Ph.D.
Current position: associate professor, Graduate School of Medicine
and
Pharmaceutical
Sciences,
University
of
Toyama
Education/Career:
1980: University of Tsukuba (~1984)
1984: Graduate School of Pure and Applied Sciences, University of
Tsukuba (~1986)
1986: National Chemical Laboratory for Industry (reorganized to
"National Institute of Advanced Industrial Science and Technology" : ~
2002)
1992: University of Oxford (UK) as a postdoctoral researcher (~1994)
2002: Universit Louis Pasteur (France) as a visiting professor
2002: Toyama Medical and Pharmaceutical University (reorganized to "University of Toyama")
Current publications:
1. Tomohiro, T., Morimoto, S., Shima, T., Chiba,

J., Hatanaka, Y. An isotope-coded fluorogenic
cross-linker for high-performance target
identification based on photoaffinity labeling.
Angew. Chem. Int. Ed. 2014, 53, 1350213505.Tomohiro, T., Yamamoto, A., Tatsumi,
Y., Hatanaka, Y. [3-(Trifluoromethyl)-3Hdiazirin-3-yl]coumarin
as
a
carbenegenerating photocross-linker with masked
fluorogenic beacon. Chem. Commun. 2013,
49, 11551-11553.
2. Aswad, M., Chiba, J., Tomohiro, T., Hatanaka,
Y. Coupling reaction of thioamides with
sulfonyl azides: an efficient catalyst-free
click-type ligation under mild conditions.
Chem. Commun. 2013, 49, 10242-10244.
3. Tomohiro, T., Inoguchi, H., Masuda, S.,
Hatanaka, Y. Affinity-based fluorogenic
labeling of ATP-binding proteins with
sequential photoactivatable cross-linkers.
Bioorg. Med. Chem. Lett. 2013, 23, 56055608.
4. Morimoto, S., Tomohiro, T., Maruyama, N.,
Hatanaka, Y. Photoaffinity casting of a
coumarin flag for rapid identification of
5.
6.
7.
8.
ligand-binding sites within protein. Chem.

Commun. 2013, 49, 1811-1813.
Tomohiro, T., Kato, K., Masuda, S., Kishi, H.,
Hatanaka, Y. Photochemical Construction of
Coumarin Fluorophore on Affinity-Anchored
Protein, Bioconjugate Chem. 2011, 22, 315318.
Masuda, S., Tomohiro, T., Hatanaka Y. Rapidly
photoactivatable ATP probes for specific
labeling of tropomyosin within the
actomyosin protein complex, Bioorg. Med.
Chem. Lett. 2011, 21, 2252-2254.
Kashiwayama, Y., Tomohiro, T., Narita, K.,
Suzumura, M., Glumoff, T., Hiltunen, J. K., Van
Veldhoven, P. P., Hatanaka, Y., Imanaka, T.
Identification of a substrate-binding site in a
peroxisomal
b-oxidation
enzyme
by
photoaffinity labeling with a novel palmitoyl
derivative. J. Biol. Chem. 2010, 285, 2631526325.
Review: Tomohiro, T., Hatanaka, Y. Diazirinebased multifunctional photo-probes for
affinity-based elucidation of protein-ligand
interaction. Heterocycles 2014, 89, 26972727.
Patent:
8 patents and 4 patent-pending
38 | P a g e

CV OF SPEAKER
Wangsa Tirta Ismaya, PhD
Education:
Universitas Padjadjaran
1993-1998 Chemistry / Biochemistry Bachelor of Sciences
Indonesia Bandung
University of Groningen
2002-2011 Biophysical Chemistry / Biochemistry PhD
Netherlands Groningen
Research Experience:
Research Scientist
April 2013-present: Dexa Laboratories of Biomolecular Sciences Biopharmaceuticals Technology
Recombinant Protein Therapeutics Research Group
Indonesia Cikarang
Postdoctoral Fellow
Feb 2012-Jan 2013 Georgia Institute of Technology Institute for Bioengineering and Bioscience
Bioengineering of Natural Products
USA Atlanta
April 2009-Jan 2012 Universiteit Utrecht Department of Biochemistry and Cell Biology (DBC)
Bifunctional Protein
Netherlands Utrecht
Research Scholar
Feb 2008-Feb 2009: University of Groningen Groningen Biomolecular Sciences and Biotechnology
Institute (GBB) Protein X-ray Crystallography
Netherlands Groningen
Teaching/Research Assistant
Bandung Institute of Technology Pusat Antar Universitas - Bioteknologi Biokimia
Indonesia Bandung
Publication:
1. Ismaya, W. T., Rozeboom, H. J., Weijn, A., Mes, J. J., Fusetti, F., Wichers, H. J., & Dijkstra, B. W.
(2011). Crystal structure of Agaricus bisporus mushroom tyrosinase: identity of the tetramer
subunits and interaction with tropolone. Biochemistry, 50(24), 5477-5486.
2. Ismaya, W. T., Rozeboom, H. J., Schurink, M., Boeriu, C. G., Wichers, H., & Dijkstra, B. W. (2011).
Crystallization and preliminary X-ray crystallographic analysis of tyrosinase from the
mushroom Agaricus bisporus. Acta Crystallographica Section F: Structural Biology and
Crystallization Communications, 67(5), 575-578.
3. Ismaya, W. T., Hasan, K., Kardi, I., Zainuri, A., Rahmawaty, R. I., Permanahadi, S., ... & Soemitro, S.
(2013). Chemical modification of Saccharomycopsis fibuligera R64 -Amylase to improve its
stability against thermal, chelator, and proteolytic inactivation. Applied biochemistry and
biotechnology, 170(1), 44-57.
4. Natalia, D., Vidilaseris, K., Ismaya, W. T., Puspasari, F., Prawira, I., Hasan, K., ... & Soemitro, S.
(2015). Effect of introducing a disulphide bond between the A and C domains on the activity
and stability of Saccharomycopsis fibuligera R64 -amylase. Journal of biotechnology, 195, 8-14.
5. Etc

39 | P a g e
LIST OF COMMITTEES
Host Organizer
Pharmacy Faculty University of Muhammadiyah Surakarta
Steering Committee
Rectorate board of UMS, Dean of Faculty of Pharmacy UMS
Organizing Committee
Chair
:
Dr. Azis Saifudin, Apt

Email : Azis.Saifudin@ums.ac.id
Mobile : +62 82135670601
Secretary
1. Dr. Anita Sukmawati, Apt

2. Alfian Mahardika F., Apt
3. Rahmadhani Tyas, Apt
Treasurer
1. Ika Trisharyanti, M.Pharm., Apt

2. Dyah Susilawati
3. Sri Rahayu
Conference
programme
1.
2.
3.
4.
5.
6.
7.
8.
9.
Dr. Erindyah Retno Wikantyasning, Apt

Arini Fadhilah, Apt
Juwita Rahmawati, Apt
Aan Wahyu Widodo
Muchamad Zein Arif
Lusiana Putri
Mahardika Putri Bestari
Sita Sofiana
Chinthia Devientasari
1.
2.
3.
4.
5.
6.
7.
8.
9.
Hidayah Karuniawati., M.Sc., Apt

Titis Putri I, Apt
Cita Hanif M, Apt
Avanilla Fany S., Apt
Fais Ayu Febrina
Umul Salamah
Siti Susilowati
Anisa Widyaratna
Vindhy Mulya Gustina
Registration and
Administration
Moderator
1.
2.
3.
4.
5.
6.
7.
8.
Dr. Zakky Cholisoh, Apt (plenary session)

Dr. Anita Sukmawati, Apt
Dr. Haryoto, M.Sc
Dr. Maryati., Apt
RatnaYuliani, M.Biotech,St
Broto Santoso, M.Sc., Apt
Gunawan Setiyadi., Apt
Suprapto, M.Sc., Apt
Scientific committee
1.
2.
3.
4.
5.
6.
7.
8.
Dr. Erindyah R Wikantyasning, Apt

Tanti Azizah, M.Sc., Apt
Dr. M. Dai, Apt
Dr. Muhtadi, M.Si.
Dr. Anita Sukmawati., Apt
Dr. Zakky Cholisoh, Apt
Dr. Azis Saifudin, Apt
Dr. Maryati, Apt
40 | P a g e

9.
10.
11.
12.
13.
14.
15.
Dra. Nurul Mutmainah, Apt

Arifah S Wahyuni, M.Sc., Apt
Dedi Hanwar, M.Si., Apt
Rima Munawaroh, M.Sc., Apt
RatnaYuliani, M.Biotech.St
Agus Purnomo, M.Biotech
Proceeding
1.
2.
3.
4.
5.
6.
7.
Tanti Azizah, M.Sc., Apt

Normaidah, S.Farm.
Ana Amalia
Ilmi Nurul Fatkhi
Devy Anwar Zhelsiana
Martha Nadhira
Pratiwi Widowati
Equipment and Room
1.
2.
3.
4.
5.
6.
7.
8.

Rahmat Partono
Y Daru Prabowo
Hadi Cahyo, Apt
Subhan Rosyad Abidi
Akhmad Nafarin
Tifan Adji Hutama
Andriyanto Saputro
Transport and
Accomodation
1.
2.
3.
4.
5.
6.
Abdul Shomad
Zaenal Mustakim
Wisnu Adi Nugroho
Ongki Arbiyanto
Muhammad Arif Maulia Husna
Agung Beny Santosa
Photograph and
Documentation
1.
2.
3.
4.
5.
Al Wathony
Awang Pribudi
Iwan Setiawan, Apt
Bagas Aji Kusuma
Muhammad Nur Prasetyo

41 | P a g e
MAP OF FACULTY OF PHARMACY
Garden
STORE
STORE
Garden
42 | P a g e

LIST OF TAXI AND HOTELS

LIST OF TAXI
Kosti Solo
Solo Sentral
Bengawan
Gelora
Sakura
Angkasa
Ph. +62-0271-856300
Ph. +62-0271-728728
Ph. +62-0271-734666
Ph. +62-0271-7004999
Ph. +62-0271-644194
Ph. +62-0271-781315
LIST OF HOTELS
Stars
Hotel
Alamat
*****
Kusuma Sahid Prince

Hotel Solo
Jl. Sugiyopranoto 20 Solo 57111

Telp: +62 (271) 646356
Fax: +62 (271) 644788
*****
Hotel Sahid Jaya Solo
Jl. Gajah Mada 82, Solo 57132

Telp: +62 (271) 644144
Fax: +62 (271) 644133
*****
The Royal Surakarta

Heritage
*****
Lorin Solo Hotel
****
Solo Paragon Hotel &

Residences
****
****
Jl. Slamet Riyadi No. 6 Solo 57111

Telp: +62 (271) 666111
Fax: +62 (271) 666530
Email: reservation
@theroyalsurakartaheritagesolo.
com
Jalan Adisucipto No 47 Solo
57174
Telp: +62 (271) 724500
Fax: +62 (271) 724400
Jl. Dr. Soetomo Solo 57125
Telp: +62 (271) 7655888
Fax: +62 (271) 7655700
Email:
info@soloparagonhotel.com
The Sunan Hotel Solo
Jl. Ahmad Yani 40 Solo 57143

Telp: +62 (271) 731312
Fax: +62 (271) 738677
Hotel Novotel Solo
Jl. Slamet Riyadi 272 Solo 57131

Telp: +62 (271) 724555
Fax: +62 (271) 724666
Email:
reservation@novotelsolo.com

43 | P a g e
Stars
***
***
44 | P a g e
Hotel
Alamat
Hotel Indah Palace

Solo
Jl. Veteran 284 Solo

Telp: +62 (271) 711011
Fax: +62 (271) 724368
Email: indahpalace@yahoo.com
Syariah Hotel Solo
Jalan Adisucipto No 47 Solo

57175
Telp: +62 (271) 711000
Fax: +62 (271) 736969
Al Madina Syariah
Hotel Solo
Jl. Duwet Raya No. 37 Pabelan

Kartasura Solo
Telp: +62 (271) 765 2975
Email: almadinasolo@gmail.com


2nd ICB-Pharma ProgrammeAndAbstractBook

Uploaded by

Document Information

Copyright

Available Formats

Share this document

Share or Embed Document

Sharing Options

Did you find this document useful?

Is this content inappropriate?

Copyright:

Available Formats

2nd ICB-Pharma ProgrammeAndAbstractBook

Uploaded by

Copyright:

Available Formats

INTERNATIONAL CONFERENCE

Pharmacy Faculty, Universitas Muhammadiyah Surakarta

Erindyah Retno Wikantyasning, Ph.D., Apt.

Team of Pharmaceutical Technology

Anita Sukmawati, Ph.D., Apt.

Team of Pharmacology and Microbiology

Azis, Saifudin, Ph.D., Apt.

Team of Clinical and Community Pharmacy

Dra. Nurul Mutmainah, M.Si., Apt.

Team of Pharmaceutical Chemistry

Dedi Hanwar, M.Si., Apt.

Team of Molecular Biology

Agus Purnomohadi, M.Sc.

ICB Pharma II 31 October 2015

Its my great pleasure to welcome you to the 2nd International Current

Azis Saifudin, PhD, Apt.

ICB Pharma II 31 October 2015

This conference is held to disseminate current methods which provide advanced

Azis Saifudin, PhD, Apt.

ICB Pharma II 31 October 2015

CHARACTERISTICS TESTING OF MICROCRYSTALLINE CELLULOSE FROM NATA

Adi Yugatama1*, Laksmi Maharani2, Hening Pratiwi2, Lingga Ikaditya3 .......................................... 4

PREPARATION OF ARTIFICIAL SALIVA FORMULATION

FORMULATION OF ETHANOL EXTRACT OF TUNJUK LANGIT (Helminthostachys

Fitrya1*, Najma Annuria Fithri1, Budi Untari1, Aprililianti1................................................................... 6

EFFECT COGRINDING ON THE PHYSICAL CHARACTERISTIC OF BINARY

Iskandar Zulkarnain1*, S. N. Soewandhi1, S. Wikarsa2 ............................................................................. 7

PREPARATION AND CHARACTERIZATION OF SUBMICRON PARTICLES OF PLGA

LIQUID BATH SOAP FORMULATION AND ANTIBACTERIAL ACTIVITY TEST

Lilis Handrayani1, Ratih Aryani1*, Indra1...................................................................................................... 9

THE INFLUENCE OF EGGPLANT PEEL EXTRACT (Solanum melongena L.) AS

Sholichah Rohmani1* ......................................................................................................................................... 10

OPTIMIZING COMBINATION OF SAMBILOTO HERBAL WATER FRACTION AND

Raymond Harris Mustafa1, Lannie Hadisoewignyo1*, Martha Ervina1, Lisa Soegianto1,

ICB Pharma II 31 October 2015

B-PHARMACOLOGY AND MICROBIOLOGY ................................................................................. 12

THE POTENTIAL OF THE BLACK RICE BRAN EXTRACT AS ANTIDIABETIC AGENT

ANTIOXIDANT ACTIVITIES OF NANOEMULSION CONTAINING EXTRACT OF

Erindyah R Wikantyasning1*, Nurcahyanti Wahyuningtyas1, Muhammad Dai1, Doni

ACUTE TOXICITY STUDY OF NANOEMULSION CONTAINING SAMBILOTO

Erindyah R. Wikantyasning1*, Nurcahyanti Wahyuningtyas1, Muhammad Dai1, Febby

ANTI-INFLAMMATORY ACTIVITY TEST OF CHRISTMAS PALM (Adonidia merrillii

Herlina1*, Fitrya1, Fithri Najma A1, Rahmawati Dwi Shafarina1 ....................................................... 15

THE EFFECT OF GIVING GLUCOMANNAN PORANG TUBER (Amorphophallus

Intan Martha Cahyani1*, Bekti Nugraheni1 ............................................................................................... 16

THE PROFILE OF MDA (malondialdehyde) LEVEL IN RATS GIVEN EXTRACT OF

PRE-CLINICALSTUDY OF Cr (III) BASED HYPOGLICEMIC SUPPLEMENT IN-TYPE

Kun Sri Budiasih1*, Kartika Ratna Pertiwi1............................................................................................... 18

SCREENING ANTIBACTERIAL POTENCY OF ENDOPHYTIC FUNGI METABOLITE

Lisa Soegianto1*, Martha Ervina1, Kevin Widjaja1, Angela Violita1 ................................................. 19

ACTIVITIES OF THE COMBINED EXTRACTS OF TEMPUYUNG (Sonchus arvensis)

Muhtadi1*, Nurcahyanti Wahyuningtyas1, Andi Suhendi1, Septi Heryani1 .................................. 20

ANTIBACTERIAL ACTIVITY OF COMBINATION OF CHLORAMPHENICOL AND

Ratna Yuliani1*, Agung Cokro Prabowo1, Yeni Maisyah1..................................................................... 21

ANTIHYPERCHOLESTEROLEMIC EFFECT OF Murbei (Morus alba L.) LEAVES

ICB Pharma II 31 October 2015

ANALGESIC EFFECT OF ETHANOLIC EXTRACT RED DRAGON FRUIT ( Hylocereus

Westi Fajrin Bayu Nugrahaini1*, Tanti Azizah Sujono1 ........................................................................ 23

EFFECTS OF TURMERIC ETHANOLIC EXTRACT ON TRIMETHYLTIN-INDUCED

Sapto Yuliani1*, Mustofa Mustofa2, Ginus Partadiredja2...................................................................... 24

ANTIDIABETIC ACTIVITY OF NANOEMULSION CONTAINING EXTRACT OF

Erindyah R. Wikantyasning1*, Nurcahyanti Wahyuningtyas1, Muhammad Dai1, Fajar

OVERVIEW ABOUT ANTIBIOTICS PRESCRIBING IN URINARY TRACT INFECTION

Hening Pratiwi1* .................................................................................................................................................. 26