EFFECTIVENESS OF CHINESE CHIVES (Allium tuberosum Rottler ex

Spreng.) EXTRACT ON BLOOD COAGULATION PROCESS

A Thesis Presented to the

Faculty of Medical Laboratory Science
of School of Health Science Professions
of St. Dominic College of Asia

In Partial Fulfillment
Of the Requirements for the Degree
Bachelor in Medical Laboratory Science

Dolly Anne S. Mendigorin

Louise Angela Morilla
Grace Lheen B. Samarita
Yuri Anthony D. Sucgang

March 2016
ABSTRACT

This study was designed to experiment and examine the effect of fresh
Allium tuberosum Rottler ex Spreng (Garlic chives) in blood coagulation process.
The extract was tested for its anticoagulant property with four different
concentrations along with the negative control and the positive control (Heparin).
The study was conducted by determining the Clotting Time (CT), prothrombin
time(PT), and the activated partial prothrombin time(aPTT). The constituents of
Chinese chives (Allium tuberosum Rottler ex Spreng) were the sulfur
compounds,

flavonoids,

alkaloids

and

phenolics

that

are

capable

of

anticoagulation of blood samples. For the Clotting Time, the researchers have
done two trials to be precise with the results. In the first and second trial of the
Clotting time, the results of the mean have shown an increasing trend. When all
are compared to the negative control all has shown significant increase on the
clotting time, being the 25% as the least prolongation and the 100% extract as
the highest, the 100% conc. is also comparable with the positive control
(Heparin). For the Prothrombin Time the researchers noticed that the results of
the Prothrombin time of the different concentrations have shown almost no
significant change compared to the negative control. We therefore conclude that
the Chinese Chives extract has a least significant effect on prolonging the
Prothrombin time. For the Activated Partial Thromboplastin Time the results
revealed a remarkable increasing trend in prolonging the clotting time in APTT. In
summary of the result of the experimentation, the clotting time is increased, the
prothrombin time has shown no significant difference, while the activated partial
thromboplastin time is also increased, we can therefore conclude that Chinese
Chives extract showed anticoagulant property especially on the intrinsic pathway,
tested by aPTT, of the blood coagulation cascade.
Keywords: Activated Partial Thromboplastin Time; Anticoagulant; Chinese
chives (Allium tuberosum Rottler ex Spreng.) extract; Blood Coagulation; Clotting
Time; Heparin; Intrinsic Pathway; Prothrombin Time.