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Anuj K Chandel
University of So Paulo
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University of Pittsburgh
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Received: 8 July 2011
INTRODUCTION
The constant demand for non-food and feed based substrates has
influenced the need to exploit sustainable and cheaper resources
for their bioconversion into value-added products of commercial
interest through basic routes of microbial bio-conversion.1 With
this objective, there have been many products obtained from
renewable resources such as biomass. Due to advancement in the
agricultural industries, millions of tons of wastes and byproducts
are generated every year that have potential as low-cost sources
of energy and material.1 4 One of these byproducts is sugarcane
bagasse, which can be used in the production of industrial
enzymes, ethanol, xylitol, organic acids, etc.4,5 Bagasse is a residue
obtained from sugarcane after it is crushed to obtain the juice used
for sugar and ethanol production. Another important sugarcane
residue is the leaves, which are usually left in agricultural fields
during sugarcane harvesting.6 8 The dried leaves, called sugarcane
trash (ST), are produced in abundance (68 tons from one hectare
of sugarcane crop).7 Generally, leaves are burnt in the fields, which
produces fly ash, severely damages soil microbial diversity, and
raises environmental concerns.
Sugarcane bagasse (SB) and sugarcane leaves/trash (SL or ST)
contain appreciable amount of cellulose and hemicellulose, which
can be de-polymerized by chemical or enzyme cocktails into simple
sugar monomers (glucose, xylose, arabinose, mannose, galactose,
etc.).7,8 Such sugar streams obtained from SB and SL can be converted into bioethanol and value-added products of commercial
significance, which has joint economic importance.4,7 10 Harnessing bagasse and leaves for industrial purposes could provide
a sustainable and economic solution for the production of biobased, value-added products such as ethanol, xylitol, organic acids,
industrial enzymes, and other products.4,5 The proposed model in
Fig. 1 shows the efficient utilization of SB or SL/ST into products
of economic importance via both biological and non-biological
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Sugarcane bagasse
AK Chandel et al.
Nonbiotechnological
Applications
Biotechnological
Applications
Pretreatment
Fermentation (SSF)
Enzymatic hydrolysis
Microbial fermentation (SmF)
Ethanol as fuel
Xylitol
Industrial enzymes
Organic acids
Other value-added products
(antibiotics, Single cell protein,
bio-hydrogen, aroma, pigments
etc)
Pyrolysis
Chemical catalysis
Chem-remediation
Biochar
Coumaric acid
Methyl cellulose
Furfural and 5-hydroxy
methyl furfural
Pyrolysis and steam
gasification etc.
Figure 1. Strategic applications of sugarcane plant. The pocessing of sugarcane in fields yield green tops and dried leaves, so-called sugarcane trash (ST).
The bagasse is products from the stem after juice extraction. Both have profound importance in biotechnological and non-biotechnological applications.
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Mild alkali
pretreatment
Sugarcane
bagasse
Industrial enzymes
Organic acids,
pigments, vitamins
Antibiotics, single
cell protein
Immobilizationcarrier
Direct carbon
source for
microbes in
SSF or SmF
systems
Pentose
sugar rich
hydrolysate
+ inhibitory
compounds
Auto
hydrolysis
or dilute
acid
Detoxification
(Chemical or
biological)
Cellulignin or
Holocellulose
Delignification
(alkali or
biodelignification)
Enzymatic
hydrolysis by
cellulolytic
enzymes
cocktail
Fermentation of
sugars (C5 + C6)
metabolizing
strains)
Xylitol
Ethanol
Organic
acids
Industrial
enzymes
Solvents,
pigments,
single cell
protein and
others.
Figure 2. Procedural steps involved in the application of SB for the formation of various industrially important products.
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Table 1.
Pretreatment conditions
Enzymatic loadings
AK Chandel et al.
Hydrolysis efficiency
References
15 FPU g1
92.04%
15
5 FPU g1
95%
24
91.7%
25
63.0%
26
81 1 g L1 total sugars
27
28
29
94.6%
30
Table 2.
Applications of SB in ethanol production using different microorganisms under various cultivation conditions
Hydrolysate composition (g L1 )
Glucose, 7.3; Xylose, 65.9; Galactose,
2.2; Acetic acid, 5.9
Glucose, 3.5; Xylose, 25.8; Arabinose
2.4; Acetic Acid 4.6
Glucose, 9.1; Xylose, 42.8; Galactose,
2.3; Arabinose, 4.6; Acetic acid, 0.8
Glucan, 41.63%; Xylan, 17.24%;
Arabinan, 0.68%
Xylose, 21.5; Arabinose, 2.95;
Glucose, 5.84
Glucose, 50.9; Xylose 33.1
Simultaneous saccharification and
fermentation (SiSF), Glucose,
80 g L1
Microorganism
Fermentation conditions
References
E. coli MM160
29.0 g L
27
3.2 g L1
31
0.34 g g1
32
S. cerevisiae D5A
23 g 100 g1 biomass
33
30 C, 150 rpm, 24 h
0.48 g g1 , 8.67 g L1
33.7 g L1
60 g L1
8
34
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Ethanol production
(g L1 or g g1 )
Table 3.
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Mode of cultivation
Product titers (g L1 or g g1 )
References
SSF
SSF
SmF
SSF
SSF
SSF
SSF
39
40
41
42
43
44
45
Bio-products
Xylanase
Laccase
Alpha-amylase
Lipase
Tannase
Pectate lyase
Inulinase
Industrial enzymes
SB has been used for the production of industrial enzymes
such as xylanase, cellulase, amylase, and laccase by certain
bacteria and fungi, employing solid-state fermentation (SSF) or
submerged fermentation (SmF) systems. Table 3 shows that SB
has been utilized for production of various enzymes under SSF
or SmF conditions. Among others, cellulase and xylanase have
been studied extensively for production from SB.5,40 Singhania
et al.46 compared cellulase production from SB with production
from other lignocellulosic materials such as wheat bran, cassava
bagasse, and rice straw under SSF by Trichoderma reesei NRRL
11 460. The maximum production of cellulase (154.58 U gds1 )
was reported from SB, followed by wheat bran, cassava bagasse,
and rice straw. The cost of cellulase plays a vital role in the success of
biorefineries. A potential technology has yet to be investigated that
can provide a feasible approach to the cost-effective production
of cellulase with high titers.
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Table 4.
AK Chandel et al.
Production of value added products of commercial interest from SB under various cultivation conditions
Organic acids
Microorganisms
SmF
SSF
SmF
SSF
SSF
SSF
SmF
Red pigment
SSF
the potential for strong commercialization. Despite this, no significant approach has been developed to harness its commercial
potential as a raw substrate. The molecular elements of microbial
metabolism using SB have not been established, which may assist
in efficient utilization of SB for products of commercial interest.
NON-BIOTECHNOLOGICAL APPLICATIONS
OF SUGARCANE BAGASSE
SB has also been used in conventional applications, including as a
cheaper source of energy. Burning it in boilers for steam generation
is the most common application in sugar and alcohol producing
industries.4 Apart from steam generation, SB plays major roles
in the electricity generation and pulp and paper production
industries.55 In one study, SB was fractionated into cellulose,
hemicellulose, and lignin by a proprietary steam explosion process,
followed by downstream purification, revealing that SB can be
utilized to produce high-value plastics.56 In another potential
application, Ou et al.57 studied phenolic acids that were released
from SB by alkaline hydrolysis at 30 C and purified with anion
exchange resin. The main component of the purified bagasse
hydrolysate was revealed to be p-coumaric acid rather than
ferulic acid. This purified product showed the same antioxidant
activity, reducing power, and free radical scavenging capacity
as the standard p-coumaric acid. In another attempt, SB was
anaerobically digested to produce methane,55 and the digested
residue and fresh bagasse were pyrolyzed separately into biochar
at 600 C in a nitrogen environment. This study suggests that
efficient use of SB under anaerobic digestion and pyrolysis to
produce biochar may be an economically and environmentally
beneficial use of agricultural wastes.55
In a different application of SB, Gonzalez et al.58 developed a
novel process for synthesis of diverse nanometric materials (silica
oxide) with specific crystal arrays as precursors to agro-industrial
wastes by employing vermicompost with annelids (Eisenia foetida).
In road transportation, it is a challenge to develop low-emission
vehicles with high specific power dealing with specific energy.
Super capacitors, or electrochemical double-layer capacitors, are
a promising high-power technology that can meet peak power
demands in fuel cell electric vehicles. Rufford et al.59 studied the
characterization and electrochemical performance of activated
carbons prepared by the ZnCl2 activation of sugarcane bagasse.
Their study showed that SB carbons prepared with a ZnCl2 ratio
of 3.5 were the most stable electrochemical performer at fast
chargedischarge rates.59
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Product titers (g L1 or g g1 )
Fermentation conditions
References
47
48
49
50
51
52
53
53
BIO-INDUSTRIAL SIGNIFICANCE
OF SUGARCANE LEAVES/TRASH
SL has yet to be explored for biological processes. The cell
walls of SL are composed of 57.5% carbohydrate, demonstrating
the potential for the bioconversion of products of commercial
significance, including ethanol as biofuel. However, the abundance
of lignin (36.1%) and silica (6.96%) may limit the industrial
and veterinary acceptability of SL.61 Regardless of the complex
chemical composition of its cell wall, SL was hydrolyzed by sulfuric
acid at varying temperatures, acid loads, hydrolysis times, and
solid : liquid ratios in a fractional factorial and central composite
design. The optimal conditions at 130 C with 2.9% w/v H2 SO4 ,
solid : liquid ratio (1 : 10) for 30 min residence time allowed
formation of xylose (56.5 g L1 ), corresponding to a recovery
of 85.1% from the hemicellulosic fraction of SL (Moutta et al.,
unpublished work). Krishna et al.6 reported ethanol production
(2% w/v) from SL employing SSF with cellulases from Trichoderma
reesei QM 9414 and S. cerevisiae NRRL-Y-132. Ferreira-Leitao et al.18
evaluated the saccharification of SL into glucose (97.2% theoretical
yield) after pretreatment with steam at 220 C for 5 min. Silva
et al.62 reported enzymatic hydrolysis yield of glucose (77.6%)
and xylose (56.8%) based upon the total structural carbohydrates
present in ball milled pretreated sugarcane leaves straw and this
sugar solution when fermented by S. cerevisiae showed 91.8%
ethanol yield under submerged fermentation conditions.
Another study of enzymatic digestibility (9598%) by the coordinated action of cellulases and hemicellulases was conducted
using SL.8 The released sugars were converted into ethanol by
S. cerevisiae 424A LNH-ST with appreciable ethanol concentration
(3436 g L1 ) and yield (92%). Singh et al.7 processed dried SL
after microbial pretreatment for the reduction of C : N ratio in
conjunction with cellulase production by selective bioagents
including fungi and bacteria. Microbial pretreatment of sugarcane
Table 5.
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Factors and procedural steps governing the commercialization of SB/ST with respect to impact on cost incurred
Mode of
application
Cultivation type
Bio-products
Direct
Indirect
Specialized
applications
Mild pretreatment
Bioremediation
Impact on cost
incurred
+
++
++
++
++
+
++
COMMERCIAL EVALUATION OF
BIO-PRODUCTS FROM SB/ST
Bio-products derived from SB/ST are considered a breakthrough to
replace chemically synthesized products in industries. In particular,
they offer tremendous opportunities for chemical industries to
develop unique functionality and marketing benefits due to their
sustainability, eco-friendly assessment and their vast availability in
nature.71 Looking at the copious amount of SB and ST in the world
and their practical feasibility for the production of value-added
products, this feedstock can be referred as biological or green
currency. Therefore, the importance of SB/ST as a sustainable
source of energy or other valuable products has become a subject
of intense research and commercial interest.4,10,12,71 However,
the market for white biotechnology based products including SB
and ST appears to be small (34%) on a global industry scale.71
Despite the socio-economic advantages, environmental benefits
and technological developments, entrepreneurs are hesitant to
invest in agro-based biotechnology units. Recently, corporate
business has shown their interest in biofuels promotion with
limited investment in research. Table 5 highlights the cost decisive
factors of various other applications of SB and ST
The bioconversion of SB/ST into value-added products such as
xylitol, organic acids, and industrial enzymes is profitable business
compared to ethanol production.4 In general, the cost of raw
material such as sugarcane and maize single handedly contributes
34% of the total cost of bioethanol production. Tabular data
(Table 6) reveals that the cost of biomass and cellulases has
maximum impact (nearly 70%) on bioethanol production. The
total cost of bioethanol production from SB/ST can be brought
down if combined approaches like cellulase production, hydrolysis
and fermentation of released sugars into ethanol can be merged
in a single unit. Traditional challenges such as detoxification and
recovery of sugars, etc. will remain part of the process for ethanol
production. Dias et al.12 performed a process simulation study
in which bioethanol was produced from sugarcane juice and SB
after pretreatment with an organosolv process with dilute acid
hydrolysis in a process integration using multi-pressure distillation
columns allowing cost reduction of hot utilities requirements. ST
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ACKNOWLEDGEMENT
We are grateful to FAPESP (THEMATIC PROJECT-BIOEN PROC:
2008/57926-4 and 2010/11258-0) and CNPq for financial support.
The authors also thank Dr Ellen C. Giese for critical reading of the
manuscript and valuable suggestions.
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AK Chandel et al.
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