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ABSTRACT
Introduction. The main limitation factor for wide use of umbilical cord blood units
(UCBs) as a source of hematopoietic progenitors for transplantation is cell dose.
International standard guidelines recommend 2 ⫻ 107/kg as the minimal nucleated cell dose
for UCB transplantation for adults and 3.7 ⫻ 107/kg for children. Therefore it is important to
the optimize donor selection and the collection method so as to achieve high cell doses. In this
study our main purpose was to determine whether obstetric factors influence UCBs collected.
Study design. The study involved 304 UCBs collected from January to December 2004.
The UCBs were collected after donor selection based on international criteria for cord
blood banking. We analyzed UCB biological features such as collected volume, total
nucleated cells (TNC), and CD34-positive cells, and obstetric factors.
Results. First, our study showed by multivariate analysis that infant weight was the main
factor that influenced biologic features of UCB collected such as total volume (P ⫽ .000), TNC
(P ⫽ .000), CD34 total count (P ⫽ .003), and CFU-GM (P ⫽ .004). Placental weight ⬎ 600 g
produced a better volume (P ⫽ .007) and increased TNC (P ⫽ .056). Gestational age ⬎ 39
weeks enhanced CD34% (P ⫽ .016). Regarding route of delivery, we found that cesarean
section produced higher volume and reduced WBC count compared to vaginal delivery,
regarding cord length, it increased TNC (P ⫽ .037). And last, we noticed that female infants
increased WBC (P ⫽ .013) and CD34⫹ total count (P ⫽ .019) more than male ones.
Conclusions. Our results confirm that volume and TNC are influenced by several
obstetric factors, such as greater infant and placental weight, predicting a better collection.
Our final aim was to use these results to improve the On the bases of our results we conclude that donor
collection procedure; we think that above-mentioned ob- selection and collection method optimization are the best
stetric factors predict a good collection. This means daily means of improving collection quality.
savings in resource such as time for anamnesis and collec-
tion, cost of each bag (20 €), time, and handling costs, REFERENCES
because we can focus on the supposed best collections. If we
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Transfusion 42: 2002
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