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Department of Chemistry, Faculty of Science and Arts, Ataturk Uni6ersity, 25240 Erzurum, Turkey
Department of Chemistry Education, Education Faculty, Ataturk Uni6ersity, 25240 Erzurum, Turkey
c
Department of Pharmacology, Medical Faculty, Ataturk Uni6ersity, 25240 Erzurum, Turkey
Accepted 9 November 2001
Abstract
The study was aimed at evaluating the antioxidant activity of aqueous extract of C. islandica. The antioxidant activity, reducing
power, superoxide anion radical scavenging and free radical scavenging activities were studied. The antioxidant activity increased
with the increasing amount of extracts (from 50 to 500 mg) added to linoleic acid emulsion. About 50, 100, 250, and 500 mg of
aqueous extract of C. islandica showed higher antioxidant activity than 500 mg of a-tocopherol. The samples showed 96, 99, 100,
and 100% inhibition on peroxidation of linoleic acid, respectively. On the other hand, the 500 mg of a-tocopherol showed 77%
inhibition on peroxidation on linoleic acid emulsion. Like antioxidant activity, the reducing power, superoxide anion radical
scavenging and free radical scavenging activities of C. islandica depends on concentration and increasing with increased amount
of sample. The results obtained in the present study indicate that C. islandica is a potential source of natural antioxidant. 2002
Elsevier Science Ireland Ltd. All rights reserved.
Keywords: Cetraria islandica (L) Ach.; Antioxidant activity; Lichen
1. Introduction
Oxygen is present in the atmosphere as a stable
triplet biradical (3O2) in the ground state and a vital
component for the survival of the human. Once inhaled, it undergoes a gradual reduction process and
ultimately gets metabolized into water. In this process,
a small amount of reactive intermediates, such as superoxide anion radicals (O2), hydroxyl radicals (OH),
nonfree radical species (such as H2O2), and the single
oxygent (1O2) are formed (Sies, 1993). Those reactive
intermediates are collectively termed as reactive oxygen
species (ROS) (Halliwell, 1995; Sato et al., 1996;
Squadriato and Peyor, 1998; Yildirim et al., 2000).
These primary derivatives of oxygen play an important
role in mediating ROS-related effects (Halliwell and
Gutteridge, 1989). ROS can easily initiate the peroxidation of the membrane lipids, leading to the accumulation of lipid peroxides. The peroxidation products by
* Corresponding author. Tel: +90-442-2311-936; fax: + 90-4422331-062.
E-mail address: igulcin@yahoo.com (I: . Gulcin).
0378-8741/02/$ - see front matter 2002 Elsevier Science Ireland Ltd. All rights reserved.
PII: S 0 3 7 8 - 8 7 4 1 ( 0 1 ) 0 0 3 9 6 - 8
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wanted to describe the antioxidant effects of C. islandica and to compare their antioxidant effects with
those commonly used as food antioxidants, such as
BHT, BHA, and a-tocopherol. In addition to this, the
components responsible for the antioxidative ability of
C. islandica are currently unclear. Hence, it is suggested
that further work could be performed on the isolation
and identification of the antioxidative components in C.
islandica.
The aim of the present study was to investigate the
antioxidant properties of C. islandica in order to evaluate its medicinal value and to point to an easily accessible source of natural antioxidants that could be used as
a possible food supplement or in the pharmaceutical
industry.
2.1. Chemicals
Ammonium thiocyanate was purchased from E.
Merck. Ferrous chloride, polyoxyethylenesorbitan
monolaurate (Tween-20), a-tocopherol, 1,1-diphenyl-2picryl-hydrazyl (DPPH.), nicotinamide adenine dinucleotide (NADH), butylated hydroxyanisole (BHA),
butylated hydroxytoluene (BHT), quercetin and trichloracetic acid (TCA) were purchased from Sigma Chemical Co. All other unlabeled chemicals and reagents were
analytical grade.
2.3. Extraction
For water extraction, 20 g sample was mixed with
400 ml distillated and boiling water by magnetic stirrer
for 15 min. Then the extract was filtered over Whatman
No. 1 paper. The filtrates were frozen and lyophilized
in lyophilizator (Labconco, Freezone IL).
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328
A0 A1
100
A0
4. Conclusion
Aqueous extract of C. islandica showed strong antioxidant activity, reducing power, DPPH radical and
superoxide anion scavenging activities when compared
with different standards such as a-tocopherol, BHA,
BHT, and quercetin. The results of this study show that
aqueous extract of C. islandica can be of use as an
easily accessible source of natural antioxidants and as a
possible food supplement or in pharmaceutical industry. However, the components responsible for the antioxidative activity of aqueous extract of C. islandica
are currently unclear. Therefore, it is suggested that
further work could be done on the isolation and identification of the antioxidative components in C.
islandica.
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