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MAN0000446
ii
Contents
Kit Contents and Storage ...........................................................................iv
Methods ........................................................................................ 4
Procedure for Purifying PCR Products ..................................................... 4
Analyzing DNA Yield and Primer Removal............................................ 8
Troubleshooting ......................................................................................... 10
Appendix .................................................................................... 11
Accessory Products.................................................................................... 11
Technical Support ...................................................................................... 12
Purchaser Notification............................................................................... 14
iii
Kit contents
Cat. no.
K3100-01
Cat. no.
K3100-02
15 mL
72 mL
23 mL
109 mL
16 mL
80 mL
15 mL
15 mL
50
5 50
50
5 50
Intended use
iv
PureLink
Binding
Buffers
PureLink
PCR Spin
Column
specifications
System
specifications
Binding Capacity:
40 g dsDNA
Column Reservoir
Capacity:
800 L
Elution Tube
Capacity:
1.7 mL
Centrifuge
Compatibility:
Capable of centrifuging
>10,000 g
Starting Material:
Elution Volume:
50 L
Separation Range:
Binding Buffer (B2)
Separation Range:
Binding Buffer HC (B3)
0.112 kb from
1040 mer primers
DNA Recovery:
>80%
Primer Removal:
>99%
Experimental Overview
Purifying
PCR
products
workflow
Methods
Procedure for Purifying PCR Products
Introduction
Required
materials
100% isopropanol
96100% ethanol
MEND
ION
AT
RECOM
Before
starting
Buffer
Cat. no.
K3100-01
Cat. no.
K3100-02
10 mL
100% Isopropanol
48 mL
100% Isopropanol
2.3 mL
100% Isopropanol
11 mL
100% Isopropanol
64 mL
96100% Ethanol
320 mL
96100% Ethanol
Continued on next page
1.
2.
3.
4.
5.
Washing
DNA
1.
2.
3.
1.
2.
3.
4.
5.
6.
Primer
removal
100 bp
37-mer primer
Example with
Binding
Buffer HC
(B3)
232 bp
25-mer primer
Troubleshooting
Observation
Low DNA
yield
Cause
Solution
PCR conditions
are not optimized
Incorrect binding
conditions
Ethanol not
added to Wash
Buffer
Incorrect elution
conditions
Primer
dimers
present
Incorrect Binding
Buffer used
Downstream
enzymatic
reactions are
inhibited
Ethanol present
in purified DNA
10
Appendix
Accessory Products
Introduction
Quantity
Cat. no.
4 96 reactions
K3100-96A
100 reactions
11304-011
100 reactions
10966-018
500 mL
10977-015
500 assays
Q32854
500 assays
Q32853
1 each
Q32857
50
12193-025
50 g
15628-019
11
Technical Support
Obtaining
support
SDS
Certificate of
analysis
12
13
Purchaser Notification
Limited use
label license:
research use
only
14
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