GTU 201 KESIHATAN & MASYARAKAT

GTU 201: HEALTH AND SOCIETY (2010/2011)

Soalan A (Jawab Semua Soalan)
1.
Bencana alam seperti tsunami yang melanda pada Disember 2004 dan
gempa bumi di Pakistan pada Ogos 2010 telah menyebabkan berlakunya penyakit.
Jelaskan bagaimanakah penyakit boleh terkesan dengan menggunakan SATU teori
penyebab penyakit beserta contoh.
(30 min/25m)
2.
Masalah gejala sosial dalam kalangan remaja sering dikaitkan dengan
pelabelan, penyimpangan dan stigma.
a.
Terangkan dengan ringkas kaitan antara pelabelan, penyimpangan dan
stigma. (10m)
b.
Jelaskan punca-punca berlakunya penyimpangan dan cara-cara mengatasi
penyimpangan dari aspek keluarga dan masyarakat. (15m)
Soalan B (Pilih SATU soalan sahaja)
3.

Jelaskan perkaitan antara sektor kesihatan dan kemiskinan (25m)

4.

Jeaskan kebaikan reformasi kesihatan terhadap wanita (25m)

5.

Terdapat 3 jenis keluarga.

a.

Terangkan peranan keluarga dan masalah kesihatan. (10m)

b.
Bandingkan baik dan buruk tiga jenis keluarga apabila menghadapi situasi
salah seorang ahli keluarga menghidap penyakit yang kronik dan dimasukkan ke
hospital. (25m)
Soalan C (Pilih SATU soalan sahaja)
6.

Faktor sosial penentu kesihatan

a.

Jelaskan dengan ringkas kepentingan Faktor Sosial Penentu Kesihatan. (10m)

b.
Jelaskan TIGA factor social penentu kesihatan dalam menyelesaikan masalah
penyakit berjangkit. (25m)
7.
En Harun berumur 65 tahun dan seorang penoreh getah. Dia menghidap
kencing manis dan darah tinggi. Dia perlu mendapatkan rawatan susulan tetapi
sering tidak hadir ke klinik untuk mendapatkan rawatan. Bagaimanakah cara untuk
mengubah tingkah laku En Harun. (25m)
8.
Pn Salmah ialah seorang ibu tunggal dan mempunyai 4 orang anak. 2 orang
anaknya menghidap penyakit mental. Dia tidak mempunyai sumber pendapatan
yang tetap. Selain itu, dia juga dipulaukan. Sebagai seorang professional kesihatan,
apakah tindakan anda untuk mengatasi masalah ini. (25m)

Final Exam Semester 1 (2009/2010)

Bahagian A: WAJIB jawab kesemua soalan

1. Pilih DUA faktor sosial penentu kesihatan dan kemudian huraikan. (30minit/25m)
2. Terdapat hubungan di antara status kesihatan dan status kemiskinan. Huraikan
kedua-dua hubungan ini dan jelaskan. (30 minit/25m)

Bahagian B: Sila PILIH SATU soalan sahaja

3. H1N1(selsema babi)- terangkan satu teori penyebab penyakit yang boleh
menjelaskan penyakit ini. (30minit/25m)
4. Pilih SATU kumpulan golongan yang tercicir dan huraikan. (30minit/25m)
5. Mengikut Mechanic, terdapat pelbagai variabel. Terangkan DUA dan huraikan. (30
minit/25m)

Bahagian C: Sila PILIH SATU sahaja

6. Nyatakan TIGA faktor yang boleh dilakukan oleh sesebuah keluarga bagi
membantu keluarga yang menghidapi masalah kesihatan. (30 minit/25m)
7. Berikan hujah dan mendapat anda jika ada yang mencadangakn kepada kerajaan
Malaysia untuk mengadakan institusi perkhidmatan kesihatan berasaskan:
i. bertunjangkan kebajikan dan percuma
ii. berasaskan bayaran
8. Terdapat satu teori yang dinamakan Teori mundur. Huraikan(30 minit/25m)

GTB 221: BASIC HEMATOLOGY

FINAL EXAM 2010/2011

5 QUESTIONS, ANSWER 3
1.
Explain hematopoiesis and the site process for hemostasis from first week
gestation to adulthood.
2.
Explain the the pathway for energy production and pathway that prevent
oxidative stress in red blood cell.

3.

a) Describe metabolism of iron and factors that affect absorption of iron.

b) Describe the role of iron in Hemoglobin synthesis.

4.

a) Describe fibrnolysis process.

b) Describe 2 roles of fibrinogen degradation product in regulating Secondary

hemostasis.
c) State 2 fibrinolysis inhibitor.
d)

Mean Reference Method X = 142

Mean New Method Y=157
Standard error= 8.3
By doing partial T test

i.

t=

ii.

t critical=

iii.

conclusion

Final Exam (2009/10)

BAHAGIAN B (ESSAY)
1.
With the diagram, describe the structure and function of the cellular
composition of blood. (30M)
2.

a) Explain the structure and function of hemoglobin.

b) Explain the.hemoglobinoxygen tension..

3.

a) List the five functions of platelet in the hemostasis. (5M)

b) Explain their functions in the blood hemostasis. (25M)

i- Fibrinolysis
ii- Protein S and Protein D
4.

a) Explain two principles of hematology analyzer. (15M)

b) Give two examples of the characteristic of blood cell which can make error during
the operation of hematology analyzer.
i-

Based on the two examples you give above, assume the two results obtained.

iiBased on the two example you give above, state two solutions that must
taken before start the operation to avoid the errors. (15M)

5.
a) A comparison studies have been conducted on the method of determining
glucose. 31 patients with the high range of glucose concentration are analyzed by
using method Y and compared with reference method X. Mean of method Y is 157
mg/dl, while mean of method X is 142 mg/dl. Standard error of the difference
between mean value is 8.3.
iii-

Determine t value.
What is t critical value at P= .05?

iii-

State the meaning of t value at P= .05.

iv-

What is the conclusion about the assessment of method Y? (20M)

Final exam (2006/07)

1. a) Define haematopoiesis
b) Draw flow chart of haematopoiesis

2. Explain the uptake, absorption and storage of Vitamin B12

3. Draw and explain the cascade diagram of coagulation

4. a) List 3 screening tests for coagulation screening test

b) Explain the principle, normal value and interpretation of one of the test

5. Briefly, explain;
a)

Pernicious anemia

b)

Iron deficiency anemia

6. Draw Lavey-Jenning and Cudum chart and from Levey-Jenning, what is the
interpretation for 1 normal control and 2 controls: normal and abnormal
GTB 217: BASIC IMMUNOLOGY
Answer 3 out of 5 questions
1.

a) With diagram, describe Type I Hypersensitivity (10m)

b) List 5 characteristics of vaccines for public health. (10m)

2.

a) Describe the mechanism of inflammation. (10m)

c)

Explain the effects of chronic inflammation. (10m)

3.

a) Explain the importance of Ig gene rearrangement.

b) Explain how phagocytes destroy a pathogen withing phagolysosome.

4. Immune response against parasite.

a) Explain the role of neutrophils in innate immunity.
b) Explain ways for the organism to evade from immune response.
5.

a) List the differences between Th1 and Th2 cell subsets.

b) List the differences between major histocompatibility molecule Class I and

Class II.
GTB 217 : IMMUNOLOGY
Final exam 2011/2012
1.

With a suitable diagram, describe mechanism of inflammation

2.

Regarding phagocytosis;

a) discuss role of chemokine on phagocytosis activity of macrophage (10 min)

b) explain how phagocyte destroys a pathogen within their phagolysosome (10 min)
3.

Inate imunity against candida albican

4.

a) influencing factor of the immunogenicity of antigen.

b)characteristic of vaccines to fulfil public health goal.

5.
With a suitable diagram, describe the differences between MHC class I and
MHC class II. 20MARKS
Final Exam (2009/2010)

1.

Hemolytic New-Born Disease (HND) during pregnancy.

2.

5 mechanism how T cells ignore the self-antigen.

3.

diGeorge syndrome.

4.

Anti-rejection therapy.

5.

Percepitation of curve.

6.

Secretory IgA in mucosal immunity.

7.

Fill the table: comparison of T and B cell activation.

1
Lymphocytes
B cell
Tcell
2
3
4

Antigen
receptor
BCR
TCR

Antigen receptor
complexed with
CD3, zeta
Ig, Ig

Co-receptor

Co-stimulator

CD4/ CD8
CR2(+CD19/CD81)

CD28(CTLA-4)
B7

SLE (explain laboratory diagnosis)

Allergic-induced asthma (diagram and explain)
Precaution to face future symptom in allergic-induced asthma.

GTB 204/3 TEKNIK BIOLOGI MOLEKUL

FINAL EXAM 20111/2012

1.
Briefly explain the properties of lambda () vector in cloning
experiment...5min
2.
Explain the properties and advantages of the eukaryotic expression vector
(5min)
3.

List the differences btween genomic and cDNA clone..(5min)

4.

application pcr in cloning

5.

mechanism of pull down 5min

6.
Describe the properties of IPTG and X-Gal; briefly explained of their use in
cloning experiment. (5min)
7.

difference southern and western blotting

8.

explain the principle and role of dialysis in protein purification

9.

a) how we get the spleen from life mouse?

b) explain the differences between de novo synthesis of DNA and salvage pathway
c) explain the principle selection of hybridoma cell in HAT medium
10.

engineer e.coli to produce hCG

a) e.coli contain expression vector

b) recombinant plasmid contain cDNA
c) T4 ligation d) rest. enzyme
e) modification enzyme

f) culture bacteria
g) extraction n purification hormone
11.
seorang ahli biologi molekul ingin mencari gene drpada sel hepa yg
mengkodkn protein pembekuan drh manusia.beliau mengetahui bhawa jujuran
sebhagian kecikl gene trsebut ada CTGGACTGACA.terangkan berbntukan dgn gmbr
rajah yng sesuai dgn kaedah untuk mndpt gene trsebut.
12.
a) define codon bias and the impact in the gene cloning and gene expression
(5min)
b) design experimental procedures on microarray assay-10 min
13.
design an experiment using SDS-PAGE and western blot to detect specific
protein from outer membrane of bacterial M. (15min)

Final Exam (2009/10)

Essay
1. With the example, describe the methods and applications of the PCR technique.
(5M)
2. Give 4 examples of the DNA polymerase and explain their function. (5M)
3. Explain the properties and functions of the eukaryotic expression vector. (5M)
4. Describe the differences between Southern blotting and Northern blotting. (5M)
5. Describe the differences between the one step and two step in site directed
mutagenesis. (5M)
6. what is inteference RNA.
7. What are applications of proteomics?
8. a) Describe what is codon bias and what impact of it into the cloning and
expression.
b) Give the methods and the advantages of assembly PCR
9. Describe the regulation involved of trp operon (9M)
10. Give the differentiation between Real-time PCR and Standard PCR.
11. Explain the principle that used in gel electropoiesis for separation the of the
gene.
12. Plasmid vector (figure) that has kan(r) and str(r) ..
- How to insert the gene?
- str(r) has EcoRI. How to isolate it?

13.

Hybridoma technology (15M)

Final exam (2002/03)

Part A
Each question 5 minutes. Answer only six questions.

1.

What are the properties of IPTG and X gal and its use in experiment?

2.

What are the properties and advantages of eukaryotes expression vector?

3.

What are the properties and use of M13 filamentous phage?

4.

List down the comparison between Northern blot and Microarray.

5.

Huraikan struktur sekunder protein.

6.

What is inteference RNA-Irna

7.

With the aid of diagram, explain cis acting sequence and trans acting protein

8.

What is the 4 main reason of DNA sequencing?

Part B
Each question 15 minutes. Answer only 2 questions

1.
You are going to clone human growth hormone. Design an experiment till you
get the protein ( you are given some key word related to the experiment- Cloning
simulation project)
2.
Explain the organization of lac operon and trp operon, and with diagram,
state out their difference regarding regulation.
3.
A molecular biologist wants to clone some biological factor regarding blood
clotting. He or she only knows about the small sequence ACTGACGCGC. Design
steps in getting the whole sequence

Part C
Each question 15 minutes. Answer only 2 questions
1.
a) What is codon bias? What are the impacts of codon bias in cloning and
gene expression?
b) Briefly describe assembly PCR and its application

2.
a) Design experiment SDS-PAGE and WESTERN blot to separate protein that
found in membran bacteria X.
b) What are proteomics?
c) With aid of flow chart, list out the steps in proteomics