01402312 I

1
1.
2. pKa pH
1
3. NaH2PO4 Na2HPO4 H
proton donor
4. pH = pKa + log([A-]/[HA])
5. phosphate buffer pKa2 = 6.8
6. methyl red
phenol red phenolphthalein

7.
pH
2 spectrophotometry
1. 350-780 nm
2. spectrum ( A =
absorbance)
3. A = 2 log %T %T = 10 A = 1
4. A 0 %T 0 100
5. A (nm)
6. max
7. max
8. A
9. max 420 nm max 620 nm
10. 2 2 ( max )
3
1. reducing
sucrose
2. monosaccharide
3. Phloroglucinol pentose xylose
4. Seliwanoff ketohexose fructose, sucrose
inulin
5.

6. o-toluidine A 630 nm
7. 10, 20, 30, 40 50 mg/100 ml
(o-toluidine) A
unknown
4 I
1. ninhydrin proline imino

2. xanthoproteic aromatic ()
tyrosine tryptophan
3. Sakaguchi arginine ( -naphthol
sodiumhypobromite)
4. cystein
5. Hopkins-Cole tryptophan
6. Molisch glycoprotein albumin sucrose positive
control
7. albumin
gelatin
8. lab 2 Biuret Bradford
9. Biuret mg/ml Bradford
g/ml
10. 0.1-0.9
5 II
1. pI 4.8 casein
2. ammonium sulfate 50% lactoglobulin

3. ammonium sulfate 100% lactoglobulin

lactalbumin
4.
5. ethanol
phosphate buffer
6. cation Zn Hg EDTA

7. anion anion trichloroacetic

HCl NaOH

 6 Partition chromatography
1. cellulose
TLC
2. tert-butanol mobile phase stationary phase ammonium
hydroxide
3. alanine, valine leucine
4. nonpolar side-chain alanine side-chain polar
Rf leucine Rf
5. Rf
6. Rf 0 1
7. equilibrate
8. equilibrate
9. ninhydrin
10. () unknown Rf alanine polar
( side-chain)