Professional Documents
Culture Documents
210
Winter Haven
of
Abstract. The
acid (vitamin C)
semiquinoid form
is the basis of
re
Official
Agricultural
Chemists,
vitamin
C is
Horseradish
Peroxidase, grade
II,
50
U/mg
Under low
(8). Peroxidase in
(2).
211
(9) to a
the
semiquinone
as
quickly as it is
140
120
< 8 O
0.39
LJ
? 60
40
2 O
20
Fig.
PPDA
1.
and
40
60
80
IOO
120
ASCORBIC ACID; Aig/tube
Proportionality
ascorbic
acid
between
time
concentration
lag
at
oxidation
four
of
oxidation
Fig. 2. pH optimum for oxidation of PPDA by HR PerH2O2. Reaction rates were measured in the spectrophotometer
212
buffer
molarity
(0.1
mg
PPDA.
The
and
10
higher
M)
space
or
under
N2
atmosphere.
Standards
and
Temperature.
Reaction temperature of 30 C
Approximately 10 ml of orange juice is centrifuged for 10 min at 2000 rpm in counter top cen
trifuge
warmed
water
to
30 C
in
constant
temperature
Reagents.
(M.W. 192.1)
in
Procedure
solution: Dissolve
mg per
100
ml
is
obtained
by multiplying
enzyme solution.
The
Reagents.
1. Phosphate
buffer,
1.0M,
pH
7.0.
Dissolve
213
store at 4C.
2. Horseradish peroxidase solution. Stock solu
tion: dissolve 10 mg HR Per in 1000 ml of 1.0 M
(0.26AA/min)
Store in
deficiency al
Procedure
Accuracy
and Reproducibility
of
the
Proposed
Literature Cited
2.
Glutathione also re
than
gluta
Chem. 15:614-616.
11. Purr, A.
12. Tillmans,
J.
1930.
Das
antiskorbutische vitamin.
Z.