Professional Documents
Culture Documents
2, 9198
Original
School of Life and Environmental Science, Azabu University Sagamihara, Kanagawa 252-5201, Japan
2
Faculty of Fukuoka Medical Technology, Teikyo University Omuta, Fukuoka 836-8505, Japan
Received 30 June, 2015/Accepted 25 September, 2015
From May 2014 to February 2015, 319 university studentsmale, n=173; female n=146of 18 to
24 years of age who carried mobile phones or computer tablets were selected as subjects.
Staphylococcus spp. were detected in 101 of 319 samples31.7%. In the present study, 11
strains of S. aureus were isolated and identified, not all of which were methicillin-resistant
Staphylococcus aureusMRSA
. Overall, 14 species were identied, with 11 strains10.9%of S.
xylosus being isolated at the highest frequency. Following this were eight strains7.9%of S.
cohnii and seven strains6.9%each of S. capitis and S. haemolyticus. Staphylococcus spp.
isolation was performed with bacterial samples obtained from the mobile phones of 22 specic
subjectsmales, n=12; females, n=10
. Staphylococcus spp. isolation was performed on days -1,
7 and 30 of the experiment. Staphylococcus spp. were positively detected one or more times in 12
subjects54.5%. In one subject8.3%, all three tests were positive. Furthermore, two tests
were positive in three25.0%
. In the eight remaining subjects66.7%Staphylococcus spp. were
detected only once. For the three abovementioned tests, we investigated the pulsed-field gel
electrophoresisPFGEpatterns of the strains derived from the mobile phone and from the
ngers of three subjects in whom the same bacterial species were isolated twice. From the cases
with similarities between strains derived from the ngers and the mobile phones and cases, with
consistency in the strains derived from the mobile phone at different times, commonality was
observed in the strains derived from the fingers and mobile phones along with chronological
uniformity in the strains derived from the mobile phones. A total of 101 Staphylococcus spp.
strains were isolated from mobile phones. According to drug susceptibility tests, 99 strains
98.0%were found to have some degree of resistance to drugsexcluding one strain each of S.
aureus and S. haemolyticus. Among these, the strain that showed the highest level of drug
resistance was one strain1.0%of Staphylococcus spp., which showed resistance to nine drugs.
The strain that showed the second highest level of drug resistance was one strain1.0%of S.
caprea, which showed resistance to seven drugs. In this manner, the drug-resistant tendencies of
Staphylococcus spp. isolated from mobile phones were observed.
Key wordsStaphylococcus spp. / Mobile phone / Antibacterial susceptibility.
The rate of use of mobile phones has increased drastically since the 1990 s, reaching 100% in 2014, such
that we are now living in an era in which practically all
92
K. FURUHATA ET AL.
1997
; however, in recent years, there have been many
studies of microbial contaminationHassoun et al.,
2004; Brady et al., 2007; Ramesh et al., 2008; Akinyemi
et al., 2009
. In particular, the results of microbiological
studies of the mobile phones used by medical professionals have indicated that these devices are potential
causes of healthcare-associated infectionsBrady et
al., 2009; Ustun and Cihangiroglu, 2012.
Based on this background, in order to understand
the extent to which bacteria on the hands of healthy
individuals migrate to mobile phones, with a focus on
Staphylococcus species that are naturally found on
human skin, we investigated the presence of these
bacteria on mobile phones.
With regard to Staphylococcus spp., methicillin-resistant Staphylococcus aureusMRSAinfection has
been pointed out to be an important social problem
Barrett et al., 1968. MRSA has previously been
detected on mobile phones used in medical treatment
locationsHassoun et al., 2004. We therefore carried
out antibacterial susceptibility tests for Staphylococcus
spp. adhering to mobile phones. Our objective was to
understand the mechanisms underlying the antibacterial
resistance of the strains of Staphylococcus spp.
isolated from the students phones.
RESULTS
Staphylococcus spp. isolated from the surface of
mobile phones
TABLE 1 shows the results for Staphylococcus spp.
and S. aureus detection from the mobile phones according
to gender. Staphylococcus spp. were detected in
93
Number of
samples
Male
173
Positive for
Staphylococcus spp.
%
5732.9
Positive for
S.aureus
%
42.3
Female
146
4430.1
74.8
Total
319
10131.7
113.4
: Number of samples
Origin
Male
Female
Total
S. aureusMSSA
46.9
716.3 1110.9
S. xylosus
712.1
49.3
1110.9
S. cohnii
610.3
24.7
87.9
S. capitis
58.6
24.7
76.9
S. haemolyticus
712.1
00
76.9
S. saprophyticus
23.4
37.0
55.0
S. sciuri
46.9
12.3
55.0
S. caprea
00
49.3
44.0
S. warneri
35.2
12.3
44.0
S. epidermidis
23.4
12.3
33.0
S. hominis
11.7
24.7
33.0
S. arlettae
00
12.3
11.0
S. pasteuri
11.7
00
11.0
S. simulans
00
12.3
11.0
58100.0 43100.0101100.0
: Number of strains:%
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K. FURUHATA ET AL.
Days
1
30
10
11
12
; positive; negative
Range
g/mL
Peak
MIC50
MIC90
g/mLg/mLg/mL
ABPC
0.125 - 128
0.25
MPIPC
0.031 - 128
0.25
0.25
CMZ
0.25 - 64
LMOX
0.25 - 128
16
0.031
0.063
0.5
0.5
32
GM
0.016 - 8
KM
0.063 - 128
SM
0.125 - 8
EM
0.031 - 128
0.25
0.25
16
LCM
0.031 - 128
0.25
0.25
TC
0.031 - 64
0.063
0.125
0.25
NFLX
CP
0.125 - 32
1 - 64
0.5
0.5
VCM
0.125 - 2
95
DISCUSSION
The use of mobile phones has spread rapidly in recent
years. In the present study, we investigated the adhesion of Staphylococcus spp., a resident of the skin ora,
to mobile phones based on the hypothesis that mobile
phones may be sources of infection, such as healthcare-associated infections, etc.Yamada et al., 2010;
Morioka et al., 2011. Although many such investigations of contamination have been reported in recent
yearsBraddy et al., 2005; Karabay et al., 2007; Brady
et al., 2011; Selim and Abaza, 2015, only one of these
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K. FURUHATA ET AL.
TABLE 5Multi-antimicrobial resistance patterns of Staphylococcus spp. from mobile phones.
No. of antibiotic
9
7
5
Resistance pattern
ABPC/ MPIPC/ CMZ/
EM/
LMOX/
KM/
NFLX/
TC
KM/
EM/
LCM/
LCM/
NFLX
ABPC/ MPIPC/
EM/
LCM/
CP
MPIPC/ LMOX/
EM/
LCM/
NFLX
Speices
No. of isolates
Staphylococcus spp.
11.0
S. caprea
11.0
S. xylosus
S. haemolyticus
Subtotal
22.0
ABPC/ MPIPC/ LMOX/
EM
S. saprophyticus
Staphylococcus spp.
LCM
Staphylococcus spp.
ABPC/ MPIPC/
EM/
LCM
S. xylosus
ABPC/
EM/
NFLX
Staphylococcus spp.
KM/
Subtotal
55.0
ABPC/ MPIPC/ LMOX
S. saprophyticus
S. cohnii
S. xylosus
ABPC/ MPIPC/
KM
S. cohnii
ABPC/ MPIPC/
LCM
S. cohnii
S. xylosus
ABPC/ LMOX/
TC
S. haemolyticus
S. caprea
S. epidermidis
Staphylococcus spp.
ABPC/
KM/
ABPC/
EM/
MPIPC/ LMOX/
NFLX
CP
NFLX
Subtotal
1514.9
ABPC/ MPIPC
S. cohnii
ABPC/ LMOX
S. capitis
S. epidermidis
S. hominis
S. sciuri
ABPC/
SM
S. sciuri
ABPC/
EM
S. sciuri
Staphylococcus spp.
S. aureus
S. warneri
S. haemolyticus
Staphylococcus spp.
S. simulans
Staphylococcus spp.
2
MPIPC/ LMOX
MPIPC/
KM
MPIPC/
EM
S. pasteuri
MPIPC/
TC
S. haemolyticus
EM/
TC
Staphylococcus spp.
Subtotal
2221.8
Total
4645.5
n101
97
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K. FURUHATA ET AL.