ANALISA

High Pressure Liquid Cromatography (HPLC)

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Column
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[HEWLETT-PACKARD] LiCrospher 100 RP-18e (125 X 4 mm), 5m
Loop = 20 L
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Quat. Pump
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Quat. Pump (G1311C)
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Flow:
1.000 mL/min
Low Pressure Limit:
0.00 bar
High Pressure Limit:
400.00 bar
Maximum Flow Gradient:
100.000 mL/min
Primary Channel:
Automatic
Stroke
Automatic Stroke Calculation:
Compress
Compressibility Mode:
Compressibility:
Stop Time
Stoptime Mode:
Stoptime:
Post Time
Posttime Mode:

Yes
Compressibility Value Set
100 10e-6/bar
Time set
4.50 min
Off

Solvent Composition
Channel Name 1 Used Percent
%
-----------------------------------A
Yes
20 Aquabidest
B
Yes
C
Yes
80 Asetonitril
D
Yes

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DAD
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DAD (G1315D)
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Peakwidth:
>0.10 min (2.0 s response time) (2.5 Hz)
Slit:
4 nm
UV Lamp Required:
Yes
Vis Lamp Required:
No
Analog Output 1
Analog 1 Attenuation:
Analog 1 Zero Offset:

1000 mAU
5%

Analog Output 2
Analog 2 Attenuation:
Analog 2 Zero Offset:

1000 mAU
5%

Signals
Signal table
Use Sig. Signal Wavelength Bandwidth Use Ref.
nm
nm
-------------------------------------------------------Yes Signal A
210
4
No
No Signal B
No Signal C
No Signal D
No Signal E
No Signal F
No Signal G
No Signal H
Prepare Mode
Margin for negative Absorbance:
Autobalance
Autobalance Postrun:
Autobalance Prerun:
Spectrum
Spectrum Store:
Stoptime
Stoptime Mode:
Stoptime:
Posttime
Posttime Mode:

100 mAU
No
Yes
None
Time set
4.50 min
Off