Antioksidan

Hasil Pengamatan

Data Standar Asam Galat

No.

XY

X2

Y2

2,259

5,103081

1,874

1,874

3,511876

1,714

3,428

2,937796

1,676

5,028

2,808976

1,536

6,144

16

2,359296

Jumlah

10

9,059

16,474

30

16,721025

Keterangan :
X = konsentrasi standar
Y = absorbansi standar

Pembuatan Kurva Standar Asam Galat

xy - x. y

A = y B. x
n

n. x2 - (x)2
= 5.16.474 10.9.059
5.30 - 100
= 82.37 90.59
150 - 100
= - 8.22
50
= -0.1644

9.059 (-0.1644.10)
5

= 10.703
5
= 2.1406

Persamaan garis : y = bx + a
y = - 0.1644x + 2.1406

r=

n. xy - x. y
}

5. 16.474 10. 9.059

8.22

- 8.22
8.77395008

= 0.94

y = - 0,1644x +
2,1406
R2 = 0,94

Perhitungan Sampel Cuka Salak

Absorbansi sampel = 1,116
Konsentrasi sampel :

0.5 ml = 0.5 gram cair = 1

= 500 mg/ 0.5 ml
= 1000 mg/ml
Absorbansi control = 2,259
Konsentrasi standar = 4 ppm
Absorbansi standar = 1,536

a. Perhitungan 1 (Kurva standar)

y

= - 0,1644x + 2.1406

1.116

= - 0,1644x + 2.14026

0.1644x = 2.1406 1.116

x = 1.0246
0.1644
= 6.23 mg/l = 0.00623 mg/ml

x 100%

Cons. Sampel
= 0.00623

x 100%

1000
= 0.000623 % GAEAC

b. Perhitungan 2
x

= Abs. Sampel

x Cons. Standar

Abs. Standar
= 1.116 x 4
1.536
= 0.73 x 4
= 2.92 mg/L = 0.00292 mg/ml

x 100%

Cons. Sampel
= 0.00292

x 100%

1000
= 0. 292
1000
= 0.000292 % GAEAC

c. Penentuan nilai IC
IC

= Abs. control Abs. sampel

Abs.control
= 2.259 1.116
2.259
= 1.143 x 100 %
2.259
= 50.59 %

x 100%

x 100%

Foto-foto saat praktikum

Hasil absorbansi
larutan standar 0 ppm (2,259)

Hasil absorbansi
larutan standar 1 ppm (1,874)

Hasil absorbansi
larutan standar 2 ppm (1,714)

Hasil absorbansi
larutan standar 3 ppm (1,676)

Hasil absorbansi
larutan standar 4 ppm (1,536)

Hasil absorbansi
sampel cuka salak (1,116)