Aseptic Techniques

Definitions

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Aseptic Technique, Definitions

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Pharmacy Technician Training Program

Aseptic Technique
Definitions
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Aseptic Technique
Certain procedures are carried out during the preparation of a sterile product in order to
minimize contamination.
Student note:
Nosocomial simply means "taking place in a hospital". Nosocomial infection is "Hospital
Borne" infection.

Contamination by Personnel
The person compounding is the most frequent cause of contamination, followed by
contaminated equipment and contaminated raw material.
Proper attitudes and strict adherence to aseptic techniques is essential to reducing the
likelihood of contamination.

Environment Contamination
Aseptic technique requires control of the environment.
Laminar Flow Hood, Laminar Airflow Workbench (LAFW), Vertical Flow Hood:
Designed to reduce the risk of airborne contamination during preparation of sterile products
Provides a constant flow of air to the controlled area
Air is filtered

Airflow
Pre-Filter
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Removes large particles, i.e., lint, dust
High Efficiency Particulate Air (HEPA) Filters:
Remove 99.97% of particles and airborne microorganisms 0.3 microns or larger
Must be replaced on a regular basis to sustain environment
Require regular maintenance (inspection and certification) every six months

Two Types of Hoods

Horizontal Hood - air flows horizontally across the work area
Vertical (biological safety cabinets) - air flows vertical (downward) onto the surface of the
work area
Used in the preparation of antineoplastic and other potentially hazardous products
Some of the air recirculated and filtered again; remainder is vented to the outside
Safer for the preparer:
Air is not blowing directly at them, avoiding the possibility of carrying potentially hazardous
particles or small droplets.

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