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This review sheet is a guide to helping you review concepts covered and equipment used in this course.
However, it is not necessarily a comprehensive guide to the content of the exam. You are responsible for
knowing all material covered in the lab course this semester, as well how to operate and read all
equipment that was used.
Lab 1: Scientific Method and Laboratory Techniques
Be able to correctly use and read the measuring tools used throughout the
course. For example: serological pipettes, micropipettes and graduated
cylinders. Know how the spectrophotometer works (i.e. what it measures,
how it is measured).
What is diffusion? What is meant by diffusion down a concentration gradient? What is dynamic
equilibrium? How does heat affect the rate of diffusion?
What is osmosis? Explain what is meant when a cells environment is referred to as hypertonic,
hypotonic, or isotonic? How does each affect a plant cell? An animal cell? How do animal cells,
plant cells, protists and bacteria each deal with the problem of osmosis?
How can an osmometer be used to test the solute concentration of a solution?
Be able to explain what pH means. Be able to describe how Paramecia and hypermastigotes
differ in their sensitivity to environmental pH.
Be able to distinguish between qualitative and quantitative data in this exercise or other
exercises.
Summarize the structural features of DNA as described in the lab manual and by your TA.
Why were DNA-containing solutions kept on ice throughout the lab exercise?
Explain the purpose of each of the following items in this exercise: baking soda, isopropyl
alcohol, salt, and shampoo.
At which optimum wavelengths do DNA and protein absorb the most light? What does the
absorbance ratio 260 nm/280 nm tell us about a DNA sample?