METABOLITE PROFILING OF ARTOCARPUS CHAMPEDEN STEMBARK FROM

SEVERAL DIFFERENT LOCATIONS IN INDONESIA AND CORRELATION WITH

TOXICITY

IMAM TAUFIK1, ADITA AYU PERMANASARI2, LIDYA TUMEWU2, MOCHAMMAD

YUWONO3, ATY WIDYAWARUYANTI2,4
1

Candidate of Master of Pharmacy, Airlangga University, Surabaya, Indonesia,

2
Institute of Tropical Disease, Airlangga University, Surabaya, Indonesia,
3
Department of Pharmacy Analysis, Faculty of Pharmacy, Airlangga University,
Surabaya, Indonesia, 4Departement of Pharmacognosy and Phytochemistry, Faculty
of Pharmacy, Airlangga University, Surabaya, Indonesia. Corresponding author email: yuwono05@yahoo.com; aty_ww@yahoo.com

Artocarpus champeden stembark (Moraceae) in Indonesia well known as

cempedak had been traditionally used for malarial remedies. Several studies
reported that this plant had in vitro and in vivo antimalarial activity but there was
no report about in vitro toxicity. The different of growth locations could cause the
different of metabolite profiling. As a consequence, there were different toxicities in
spite of from the same plants. The aim of this research was to obtain the profile of
metabolites that contained in A. champeden stembark from different locations in
Indonesia for safety purpose of this extract. The profiling had been performed by
HPTLC-Densitometry technique and toxicity had been also determined by MTT-ELISA
technique. The correlation between metabolite profiling and toxicity had been
determined by Principle Component Analysis, Hierarchical Clustering Analysis and
Partial Least Square. As a result, there is correlation between the different
metabolite profiling and toxicity from the same plant but different growth locations.

Keywords: Artocarpus champeden Spreng, Metabolite profiling, Toxicity, Multivariate

Analysis