ABSTRACT

Hydrogel sea cucumber (Stichopus variegatus) is known to contain a variety

of active ingredients which are very useful for human beings. Antibacterial activity of
the hydrogel sea cucumber has become a source of new drugs can be developed
because the potential is big with a high level of diversity and uniqueness of the new
compounds were found in particular natural sea organisms. The purpose of this study
is to determine the antibacterial activity of hydrogels cucumbers as medikamen the
root canal. Antibacterial hydrogels sea cucumber (Stichopus variegatus) gel
compared with calcium hydroxide (Ca(OH)2) which is material "gold standard" in
endodontic treatments. In this study, carried out comparative antibacterial ability of
calcium hydroxide (Ca(OH)2) and hydrogels sea cucumber (Stichopus variegatus)
0.2% by displaying their solution against bacteria Enterococcus faecalis (E.faecalis)
ATCC 29212 and isolate Clinic which have been mixed with liquid BHI media, then
incubated for 3 hours, 24 hours, 48 hours with the temperature 370C. The results
showed that overall there is a significant difference in bacterial growth between 8
E.faecalis treatment group, which made the control work BHI, BHI with bacteria
E.faecalis ATCC 29212 and clinical isolates as a positive control, and treatment of
bacterial biofilm E. faecalis ATCC 29212, biofilm E.faecalis clinical isolates,
cucumbers hydrogel coating, coating of Ca(OH)2, hydrogel + E.faecalis ATCC 29212
sea cucumber, sea cucumber + E.faecalis hydrogel clinical isolate, Ca(OH)2 +
E.faecalis ATCC 29212 and Ca(OH)2 + E.faecalis Clinical isolates, obtained
significant results with values of p <0.05. In this research also uses the time behavior
that is 3 hours, 24 jamdan 48 hours. The overall results obtained in 24 hours that is an
improvement on the antibacterial hydrogel sea cucumber (Stichopus variegatus)
compared to 3 hours and 48 hours. This is because during the 24 hours it is time
prematurasi bacteria. From the above it can be concluded that hydrogels sea
cucumber (Stichopus variegatus) to eliminate bacteria Enterococcus faecalis biofilm
adhesion.
Keywords: hydrogels sea cucumber (Stichopus variegatus), Ca (OH) 2, Enterococcus
faecalis