Professional Documents
Culture Documents
(Plant Propagation)
Kansih Sri Hartini
Sexual Propagation
Mainly applied, to conserve the richness of the
gene pool and develop new varieties, provides
opportunity for variation and evolutionary
advancement.
On the production side, this method cannot
satisfy the requirements for production quality
and quantity
It carries unpredictable characteristics of its
own, which includes the tendency to revert to
a wild-like state of the species
Production of growth
promoters
Germination
STAGE
EVENTS
Rehydration imbibition of water.
RNA & protein synthesis stimulated.
Increased metabolism increased respiration.
Hydrolysis (digestion) of food reserves by
enzymes.
(e) Changes in cell ultrastructure.
(f) Induction of cell division & cell growth.
PREGERMINATION
(a)
(b)
(c)
(d)
GERMINATION
Respiration
Initially anaerobic
Later aerobic
Soluble sugars
ATP
RNA activated
Polysomes
Protein synthesis (0.5h)
Enzymes (proteins)
DNA synthesis (45h)
http://www.rbgsyd.nsw.gov.au/
Mitosis (70h)
2008 Paul Billiet ODWS
Asexual Propagation
an exact copy of the genome of a mother plant is
made and continued in new individuals
using part of two or more plants in a union or parts
of the same plant. In the first case we call it graftage
and in the second case we call it rooting
aims at the identical reproduction of plants with
desirable features such as high productivity, superior
quality, or high tolerance to biotic and/or abiotic
stresses, and as such, plays a very important role in
continuing a preferred trait from one generation to
the next
Cuttings/Stek
Grafting
the technique of combining two or more
different plants, has been practised for
many centuries
The most common grafting techniques are
top-wedge grafting, splice grafting, whip
and tongue grafting and approach grafting.
The most common budding techniques are
T- and patch budding.
Grafting techniques
Layering
The term layering is used for all types of
propagation in which roots are formed
while the stem is still attached to the
mother plant
often used in species that are particularly
difficult to root
The most common layering techniques for
trees include: air layering, simple layering
and stooling
Micropropagation/in-vitro
propagation
terms used for procedures to propagate plants from
plant cells, tissues or organs under aseptic
conditions in a controlled artificial environment
Since this technique requires a substantial
investment in infrastructure, equipment and
materials, its application is mostly justified in the
case of high value plants where traditional
vegetative propagation methods are considered
unsuccessful
Not all plants can be successfully propagated
through micropropagation
Referensi:
Hannah Jaenicke and Jan Beniest, 2002.
Vegetative Tree Propagation in Agroforestry
Training Guidelines and References. ICRAF.
Printed by: Kul Graphics Ltd, Nairobi, Kenya.
USAID. 2007. Vegetative Propagation
Techniques Perrenial Crop Support Series
Jalalabad, Afghanistan. Publication No.
2007-003-AFG.