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and whereas the attached publication of the Bureau of Indian Standards is of particular interest
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timely dissemination of this information in an accurate manner to the public.
1 +, 1 +
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! > 0 B
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( Reaffirmed 2012 )
IS : 1051- 1980
Indian Standard
SPECIFICATION FOR
PYRETHRUM
EXTRACTS
( Second Revision )
Pest Control Sectional Committee,
AFCDC 6
Representing
Chairman
Directorate
of Plant Protection,
Quarantine
&
Storage ( Ministry of Agriculture ), Faridabad
DR K. D. PAHARIA
Members
DR N. S. AGARWAL
Ministry of Agriculture ( Department of Food )
DR K. KRISHNAMURTHY( Alternate )
DR H. L. BAMI
Central
Forensic
Science
Laboratory
( Central
Bureau of Investigation ), New Delhi
DR B. BANERJE~
Tea Research Association, Calcutta
DR G. SATYANARAYANA( Alternate )
DR N. K. BASU
The Alkali and Chemical Corporation
of India
Limited, Calcutta
SHRI S. R. NENE ( Alternate )
DR A. K. BHATNAGAR
Hindustan Insecticides Limited, New Delhi
SHRI C. C. ABRAHAM ( Alternate )
Pesticides Association of India, New Delhi
DR S. S. CHADHA
CHIEF PLANT PROTECTIONOFFICER Department
of
Agriculture,
Government
of
Maharashtra, Bombay
CHEMIST
INCHARGE,
INSECTICIDES
TESTING
LABORATORY( Alternate )
SHRI V. G. DESHPANDE
Ciba-Geigy of India Limited, Bombay
SHRI E. A. ALMEIDA ( Alternate )
SHRI OM P. DHAMIIA
Export Inspection Council of India, New Delhi
SHRI P. P. RANGA RAO ( Alternate )
National Malaria Eradication Programme, Delhi
DR M. S. DHATT
Department of Agriculture,
Government of Uttar
DIRECTOROF AGRICULTURE
Pradesh, Lucknow
DEPUTY DIRECTOR ( PLANT
PROTECTION) ( Alternate )
Plastic Containers Sectional Committee,
MCPD 11
SHRI V. DORAIRAJ
( IS1 )
Indian
Pharmacopoeia
Committee
( Ministry of
DRUGS CONTROLLER( INDIA )
Health and Family Welfare ), New Delhi
SHRI M. RAVIKANT ( Alternate )
( Continued on pug8 2 )
INDIAN
STANDARDS
INSTITUTION
This publication is protected under the Indicm Copyright Act ( XIV of 1957 ) and
reproduction in whole or in part by any means except with written permission of the
publisher shall be deemed to be an infringement of copyright under the said Act.
IS : 1051 - 1980
( Continuedfrom
page 1 )
Representing
Members
SHRI G. D. GOKHALE
SHRI V. V. KETKAR ( Alternate
Bombay
Chemicals
Private Limited,
Bombay
DR S. C. SRIVASTAVA ( Alternate )
National
Organic
Bombav
SHRI S. G. KRISHNAN
Chemical
Industries
Limited,
DR J. S. VERMA ( Alternate )
DR V. LAKSHMINARAYANA
Directorate
of Plant Protection,
Quarantine
&
Storage ( Ministry of Agriculture ), Faridabad
SHRI V. C. BHARGAVA( Alternate )
Directorate
General
of Technical
Development,
SHRI S. K. LUTHRA
New Delhi
SHRI S. C. BAJAJ ( Alternate )
Regional Research Laboratory ( CSIR ), Hyderabad
DR J. MADHUSUDHAN RAO
BASF India Limited. New Delhi
DR J. C. MAJUMDAR
DR B. P. CHANDRASEKHAR( Alternate J
Research Institute
SHRI S. K. MAJUMDAR
\ Central Food Technological
( CSIR ), Mysore
SARI M. MUTHU ( Alternate )
SHRI K. S. MEHTA
Bharat Pulverising Mills Private Limited, Bombay
SHRI S. CHATTERJI( Alternate )
Agromore Limited, Bangalore
SHRI L. S. MIRLE
Pesticides Formulators Association of India (S.S.I.),
SHRI J. M. MODI
Bombay
DR S. R. BAROOAH ( Alternate j
DR A. L. MOOKERJEE
Cyanamid India Limited, Bombay
SHRI J. P. PARIKH ( Alternate )
Agricultural Chemicals Division, Indian Agricultural
Da S. K. MUKERJEE
Research Institute ( ICAR\), New Delhi
DR N. K. ROY ( Alternate )
SHRI K. R. NARAYANA RAO
Food Corporation of India, New Delhi
SMT K. K. M. BHAVNANI ( Alternate )
SHRI I. NARSAREDDY
Department of Agriculture, Government of Andhra
Pradesh, Hyderabad
SHRI C. DHARMA RAO ( Alternate )
( Continued on page 18 )
IS : 1051 - 1980
Indian
Standard
SPECIFICATION FOR
PYRETHRUM EXTRACTS
( Second
0.
Revision. )
FOREWORD
0.1 This Indian Standard ( Second Revision ) was adopted by the Indian
Standards Institution on 28 October 1980, after the draft finalized by the
Pest Control Sectional Committee had been approved by the Agricultural and Food Products Division Council and the Chemical Division
Council.
0.2 This standard was first published in 1957. The first version in 1973
incorporated two amendments of flash point and packing requirements.
In this second version mercury reduction method has been incorporated
A qualitative method based on thin
for the determination of pyrethrins.
layer chromatography
( TLC) has also been incorporated.
.
to contain
2 percent
0.5 In the preparation of this standard due consideration has been given
to the provisions of the Insecticides Act, 1368 and the rules framed
However, this standard
is subject to the restrictions
thereunder.
imposed under these rules wherever applicable.
3
--
._
IS:
1051-1980
method of
percentages
2. REQUIREMENTS
2.1 Description
and Identity
IS : 1051-
Value, Percent
UP to
the tolerance
Tolerance
+10
Limit,
1980
limits
Percent
-5
-3
of
the nominal
value
2.4.2 The average content of all samples taken shall not be lower than
the nominal content.
2.5 Additional Requirement for the Material for Use as an Aerosol The matter insoluble in dichlorodifluoromethane
in the material meant
for use as an aerosol, shall not exceed 15 percent by mass when determined by the method prescribed in Appendix C.
3. PACKING
AND MARKING
given
a in IS : 8190 (Part
3.2 Marking - The containers shall bear legibly and indelibly the
following information and any other information as is necessary under
the Insecticides Act and Rules:
a> Common
b)
cl
4
e)
f1
g)
notice as worded
in the Insecticides
Mark.
*Requirements for packing of pesticides : Part II Liquid pesticides (,jht
5
revision).
IS :
1051- 1980
NOTE - The use of the IS1 Certification Mark is governed by the provisions of the
Indian Standards Institution ( Certification Marks ) Act and the Rules and Regulations made thereunder.
The IS1 Mark on products covered by an Indian Standard
conveys the assurance that they have been produced to comply with the requirements
of that standard under a well-defined system of inspection, testing and quality
control which is devised and supervised by IS1 and operated by the producer.
IS1
marked products are also continuously checked by IS1 for conformity to that standard
as a further safeguard.
Details of conditions under which a licence for the use of
the ISI Certification Mark may be granted to manufacturers or processors, may be
obtained from the Indian Standards Institution.
4. SAMPLING
4.1 Representative
samples of the material shall be drawn as prescribed
in the Indian Standard methods for sampling of pesticides and their
formulations ( under preparation ).
NOTE - Till such time the standard under preparation is published,
shall be drawn as agreed to between the concerned parties.
the samples
5. TESTS
5.1 Tests shall be carried
in 2.1.2 and 2.3 to 2.5.
methods referred to
chemicals
that
do not contain
impurities
APPENDIX
A
( Clause 2.1.2)
IDENTITY TEST FOR PYRETHRINS
A-O. PRINCIPLE
A-0.1 The method is based on the thin layer chromatographic
of pyrethrins
A-l.
separation
if any.
APPARATUS
use (
coated
with 038 mm
second revision ).
A-l.2 Accessories
A-1.2.1 Applicator A-1.2.2 Pipettes -
standard.
5 microlitres.
glass.
standard.
A-2. REAGENTS
A-2.1 Developing Solvent -
V/V).
A-2.2 Iodine-Crystals
A-2.3 Methyl Alcohol
A-2.4 Standard Pyrethrum Oleoresin - of known total pyrethrin content.
, A-2.5 Petroleum
Ether (40 - 60 ) bp
IS : 1051 - 1980
A-3.3 Thin Layer Chromatography
- Place the deveIoping solvent in the
developing tank so that layer of the solvent is 15 cm from the bottom
of the tank. Cover with lid and allow to stand for 1 hour.
A-3.3.1 Using a template, mark the TLC plate with a sharp instrument
as shown in Fig. 1. Keep the marked plate in an oven at 110C for I5
minutes and then allow it to cool to room temperature.
!
I
II
z
-4
1.5
All dimetdons
FIG, I
in centimetres.
SAMPLE
FOR TLC
A-3.3.3 Allow the solvent from the spats to evaporate and then keep
this plate in the developing tank. Allow the solvent front to reach the
top line and then remove the plate and allow the solvent to evaporate.
8
IS : 1051 - 1980
AND
developed
after
INFERENCES
A-4.1
APPEND-IX
B
( Clnuses2.1.2and 2.4)
DETERMINATION
OF TOTAL PYRETHRIN
CONTENT
B-O.PRINCIPLE
B-O.1 The pyrethrins
REAGENTS
1s:1051-1980
add 75 ml hydrochloric
acid and 5 ml chloroform and adjust to faint
iodine colour (in chloroform)
by adding dilute potassium iodide or
potassium iodate solution.
If much iodine is liberated, use stronger
solution of potassium iodate than 001 M at first, making final adjustment with 001 M solution. Keep in dark and readjust when necessary.
It should not be stored in refrigerator.
B-l.3 Potassium Iodate Standard Solution - 001 M. Dissolve 214 g
pure potassium iodate, previously dried at 105C in water and dilute to
one litre. One ml of solution = 0005 7 g pyrethrin I.
B-I.4 Alcoholic Sodium Hydroxide
B-1.5 Petroleum
60-80C.
B-l.6
aromatic
Ether -
Ethyl Ether -
Solution free,
1 N and 05 N.
boiling
range
40-60C
or
peroxide free.
Standard Solution -
002 N.
Dilute
Sulphuric
B-l.11
Ethyl
Alcohol
Acid -
10 percent (m/v).
1 : 4 ( v/v ).
95 percent
(V/V)
and
99 percent
( v/v ),
anhydrous.
B-1.12 Sodium Chloride -
solution.
B-1.13 Chloroform
B-1.14 Dilute Hydrochloric
B-1.15 Pbenolphthalein
ethyl alcohol.
Acid-3
Indicator
: 2 (v/v).
Solution -
one
percent
( m[v)
in
B-2. PROCEDURE
B-2.0 Weigh sample containing 40-150 mg total pyrethrins, add 50 ml
petroleum ether and 1 g filter ccl, and place in refrigerator at 0 f 05C
overnight.
Filter through Gooch into 300 ml Erlenmeyer flask and
wash with three 15 ml portions of cold petroleum
ether. Evaporate
filtrate and washings on water bath, using air current until the petroleum
ether is almost entirely removed.
Add 20 ml 1 N alcoholic sodium hydroxide, or more if necessary,
to extract pyrethrins, connect to reflux condenser, and boil gently for
60 to 90 minutes.
Transfer to 600-ml beaker and add enough water to
make aqueous layer 200 ml. If more than 20 ml alcoholic sodium
B-2.1
10
IS : 1051 - 1980
hydroxide
solution
was used,
add enough
water
so that all
alcohol is removed when volume is reduced to 150 ml. Add few glass
beads and boil aqueous layer down to 150 ml. Transfer to 500-ml
separator and drain aqueous layer into 250-ml volumetric flask. Wash
oil layer once with water and add washings to aqueous portion.
If
slight emulsion still persists after draining aqueous layer and washings,
add two to three ml 10 percent barium chloride solution but do not
shake vigorously after adding barium chloride, because reversed emulsion
difficult to separate may form. To aqueous solution in 250-ml flask add
1 g filter ccl and approximately 10 ml of the barium chloride solution.
Swirl gently and let stand for 30 minutes. Dilute to volume; mix
thoroughly and filter off 200 ml. Test filtrate with barium chloride
solution to see if enough has been added to obtain clear solution.
Neutralize with sulphuric acid ( 1 : 4 ), using 1 drop phenolphthalein
and
add 1 ml excess. ( If necessary to hold solution overnight at this point,
leave in alkaline condition. )
B-2.2 Determination
of Pyrethrin I - Filter acid solution from B-2.1
through 7 cm paper, coated lightly with suspension of filter ccl in water,
on buchner, and wash with three 15 ml portions of water. Transfer to
500-ml glass stoppered separator and extract with two 50 ml portions
petroleum ether. Shake each extract approximately
for one minute,
releasing pressure if necessary by inverting separator and carefully
venting through stopcock.
Let layers separate for approximately
five
minutes or until aqueous layer is clear before draining and re-extraction.
Reserve aqueous layer for pyrethrin II determination.
Do not combine
petroleum ether extracts but wash each in sequence with same three
10 ml portions water, and filter petroleum ether extracts through small
cotton plug, into a clean 250-ml separator.
Wash separators and cotton
in sequence with 5 ml petroleum ether. Extract combined petroleum
ether solutions with 5 ml 01 N sodium hydroxide, shaking vigorously
for approximately one minute. Let layers separate for approximately
5
minutes before draining aqueous layer into IOO-ml beaker.
Wash
petroleum ether with additional 5-ml portion 01 N sodium hydroxide
and with 5 ml water, adding washings to beaker. Add 10 ml Deniges
reagent and let stand in complete darkness for one hour at 25 -1_2C.
Add 20 ml alcohol and precipitate mercurous chloride with 3 ml
saturated sodium chloride solution.
Warm to approximately
60C and
let stand several minutes until precipitate coagulates and settles. Filter
through small paper, transferring all precipitate to paper, and wash with
approximately 10 ml hot alcohol.
Wash with 2 or more 10 ml portions
hot chloroform and place paper and contents in 250-ml glass stoppered
conical flask. Add 50 ml cooled dilute hydrochloric acid ( 3 : 2 ). Add
5 ml chloroform and 1 ml freshly adjusted iodine monochloride
solution
and titrate with standard potassium iodate solution, shaking vigorously
approximateIy
30 second after each addition, until no iodine colour
11
IS : 1051 - 1980
remains in chloroform layer. Take as end point when red colour disappears from solvent layer and does not return within three minutes.
From standard potassium iodate solution used in titration and blank on
Deniges reagent, calculate percent pyrethrin I.
B-2.3 Calculation
NOTE - Chrysanthemum
monocarboxylic
acid reacts with Deniges reagent to
form series of colours beginning with phenolphthalein
red, which gradually changes
to purple, then to blue and finally to bluish green. Colour reaction is very distinct
with 5 mg monocarboxylic
acid and amounts as low as 1 mg can usually be
detected.
Therefore,
no pyrethrin 1 should be reported if colour reaction is
negative.
With samples containing much perfume or other saponifiable ingredients,
it may be necessary to use as much as 50 ml 1 N alcoholic sodium hydroxide.
When lethanes are present, after washing mercurous chloride precipitate
with
alcohol and chloroform, wash once more with alcohol and then several times with
hot water.
B-2.4 Determination
of Pyrethrin
II - Tf necessary,
filter aqueous
residue reserved in B-2.2 from petroleum ether extract through Gooch.
Concentrate filtrate to approximately 50 ml and transfer to 500-ml glass
stoppered separator.
Wash beaker with three 15 ml portions water.
Acidify with 10 ml hydrochloric acid and saturate with sodium chloride.
(Acidified aqueous layer must contain visible sodium chloride crystals
throughout following extractions.)
Extract with 50-ml ether, drain aqueous layer into second separator and
extract again with 50 ml ether. Continue
extracting
and draining
aqueous layer, using 35 ml for third and fourth extractions.
Shake each
extract for approximately one minute, releasing pressure, if necessary,
by inverting separator and carefully venting through stopcock.
Let
layers separate for approximately five minutes or until aqueous layer is
clear before subsequent draining and extraction.
Combine ether
extracts, drain and wash with three 10 ml portions saturated sodium
chloride solution.
Filter ether extracts through cotton plug into 500-ml
conical flask and wash separator and cotton with additional 10 ml ether.
Evaporate ether on water bath, and remove any fumes of hydrochloric
acid with air current and continue
heating for approximately
five
minutes.
Dry for 10 minutes at 100C. Add 2 ml neutral alcohol and
20 ml water and heat to dissolve acid. Cool, filter through Gooch if
necessary, add two drops phenolphthalein and titrate with 002 N sodium
hydroxide.
Check normality of 002 N sodium hydroxide on the same
day, as the sample is titrated.
B-2.5
Calculation
II.
IS : 1051 - 1980
APPENDIX
C
( Cluuse 2.5 )
DETERMINATXON OF MATTER INSOLUBLE
DICHLORODIFLUOROMETHANE
C-l.
IN
APPARATUS
C-l.1
13
L.-.-.
-~~____~_~~_~~_.~~~~~
.._
.._
.~
__
IS : 1051- 1980
base.
Suficiently
Fig. 2.
C-1.1.2 Pressure Cap, B - Constructed from a 30 mm round brass
stock by machining on I a turning lathe to the same shape as the glass
stopper furnished with the bottle.
The lower end of the cap is then
FIG. 3
VERTICAL
SECTION
14
IS : 1051- 1980
IS : 1051 - 1980
C-2.3
Dichlorodifluoromethane
C-2.4
Chloroform
C-3. PROCEDURE
C-3.1 Preparing the Apparatus - Fill the filter screen D with lambs
wool. Wash the filter screen, the pressure cap B and the needle valve C
first with acetone and then with chloroform.
Clean the bottle A with
ethyl alcohol-sulphuric
acid mixture and then rinse several times with
water. Dry the bottle, filter screen, pressure cap and the needle valve
in an oven at 105 f 1C for one hour and cool in a desiccator.
C-3.2 Weigh accurately about 6 g of the material into the glass bottle
Fit the filter screen D,
A and place it in its position in the apparatus.
the needle valve C and the neoprene washer N to the pressure cap .B and
place in the top of the bottle.
Place the ball-bearing E between the
tightening screw H and the pressure cap B and tighten the screw. Place
the safety shield L over this assembled unit. Add 294 j, 1 g of liquid
dichlorodifluoromethane
to the bottle A by following the method given
under C-3.2.1.
C-3.2.1 Open the needle valve C, connect it to a vacuum pump and
evacuate the bottle A to at least 635 cm of mercury.
Close the needle
valve C and disconnect the vacuum pump. Place the assembly of the
apparatus after evacuation on a suitable balance.
Connect the needle
valve C to the source of liquid dichlorodifluoromethane
by means of a
suitable hose, counterpoise the apparatus by placing necessary weight on
the other pan of the balance and then place additional weight equivalent
to 294 g on it. Open the needle valve C to allow dichlorodifluoromethane flow into the bottle A and close it when the necessary quantity has
been added. Disconnect the source of dichlorodifluoromethane
and
remove the hose from the needle valve C.
C-3.3 Place the assembly of the apparatus, after adding dichlorodifluoromethane, in a rack so as to allow the bottle A to rest at an angle
of 300. At intervals of 10 minutes over a period of two hours, rotate
the assembly of the apparatus in the rack to approximately
45. Allow
the assembly of the apparatus to remain in the rack overnight. Remove
the dichlorodifluoromethane
by holding the needle valve C downward
and releasing the liquid slowly by opening the needle valve. When all
the liquid has been discharged,
weigh 150 f 1 g of dichlorodifluoromethane into the bottle A by the method used earlier, rinse the
contents of the bottle by shaking and completely remove the dichlorodi*methane
as before.
f .
16
rs : 1051- 1980
C-3.4 Remove the pressure cap B from the bottle A and detach the
filter screen D from it. Place the filter screen in a lo-ml beaker, add 5
to 7 ml of chloroform and allow to stand.
Add 15 ml of chloroform to
the bottle A and rotate while holding it in a horizontal position.
Transfer the contents of the bottle to a tared 125-ml Erlenmeyer
flask. Wash the bottle four times with 15 ml portions of chloroform
flask. Transfer the
and transfer each washing to the Erlenmeyer
chloroform contained in the lo-ml beaker to the Erlenmeyer flask.
Wash the beaker and the filter screen D with a few 5-ml portions of
chloroform and transfer the washings to the Erlenmeyer flask either by
distillation or by heating slowly on a steam-bath.
Place the flask in an
oven maintained at 105 f 2C and dry the residue for one hour. Cool
the flask containing the dry residue in a desiccator for two hours and
weigh. Note the mass of the dry residue contained in the Erlenmeyer
flask.
C-4. CALCULATION
Matter insoluble in dichlorodifluoromethane,
in the material, percent by mass
_ 100m
it4
where
m = mass, in g, of the dry residue ( see C-3.4); and
it4 = mass, in g, of the material taken for the test
( see C-3.2 ).
17
IS : 1051- 1980
( Continuedfrom
page 2 )
Members
Representing
DR V. N. NIGAM
Mimstry of Defence ( DGI )
SHRI P. N. AGRAWAL ( Alternate )
Department of Agriculture, Government
SHRI M. M. PADALIA
Ahmadabad
SHRI R. Cr. JADEJA ( Alternate )
DR S. Y. PANDIT
Bayer (India) Limited, Bombay
SHRI D. N. NAKHATE ( Alternate )
Ministry of Defence ( R & D )
SHRI C. F. PAUL
SHRI M. G. CHATTERJEE( Alternate )
DR P. S. PHADKE
Sandoz (India) Limited, Thane
SH~I A. V. NAIXJ ( Alternate )
SHRI S. M. PRADHAN
Metal Containers Sectional Committee.
\,
of Gujarat,
MCPD
12
--~I
SHRI Y. A. PRADHAN
Ralbs India Limited, Bombay
DR 2. J. KAPADIA ( Alternate )
Tata Chemicals Limited. Mithapur
SHRI D. N. V. RAO
SHRI C. NEELKANTHAN( Alternate )
DR K. K. SAXENA
Indofil Chemicals Limited, Bombay
SHRI G. NATARAJAN( Alternate )
SECRETARY
Central Insecticides Board, Directorate
of Plant
Protection, Quara,ntine & Storage ( Ministry of
Agriculture ), Faridabad
DR R. C. GUPTA ( Alternate )
AVM J. K. SEHGAL
Directorate
General,
Armed Forces
Medical
Services, Ministry of Defence
LT-COL P. S. GILL ( Alternate )
SHRI A. C. SHROFF
Excel Industries Limited, Bombay
SHRI P. V. KANGO ( Alternate )
DR K. S. SINGH
Indian Veterinary
Research
Institute
(ICAR),
I7atnaear
---.__._~~_
SHRI S. C. SRIVASTAVA( Alternate )
SHRI N. S. VENKATARAMAN
Department of Agriculture,
Government
of Tamil
Nadu, Madras
DR P. R. VENKATESWARAN
Union Carbide (India) Limited. New Delhi
DR K. N. SHRIVASTAVA( Alternate > _
.
SHRI E. R. VICCAJEE
Paper and Flexible Packaging Sectional Committee,
MCPD 14 ( IS1 )
SHRI N. G. WAGLE
Pest Control (India) , Private Ltd, Bombay
SHRI M. R. BAJIKAR ( Alternate )
DR B. L. WATTAL
SHRI G. C. JOSHI ( Alternate
SHRI T. PURNANANDAM,
Director
National Institute
Delhi
)
Director
of
Communicable
Assistant
Director
18
Member )
Diseases,
_/..e*..
.-_
.-.-.-.
;,
A~~ENDMENT ~0. 1
APRIL 1985
TO
IS: -lO!il-1980
Revision)
.. -._.
::,yT
(page
6,
4,l
Representative samples of the material ahall.bc
drawn as prescribed in IS:10627-1983 Methods for
sampling of pesticidal formulations,1
'A-2.6 &ynthetiE
pethroid
------
(Page 8, clause
matter at the end:
A-3.3.2)
A-3.3.3
Allow the solvents from the spots to evapombe
and keep this plate in the developirg tank at 20O to
Allow the plate to develop for about 110minutes
35Oc.
when the solvent front reached the top line unifonW.
Remove the plate and allow the solvents to evaporate
completely by keeping it at about 800~ for 10 mint.rk::s.
Keep the plate in iodine vapours when the spots zre
developed after an hour.'
L--__-.
-__-.
(AFCDC 6)
. ,- .
_.__._.-.
~._..
..--.----- ----
AMENDMENT
NO. 2
NOVEMBER
1987
TO
Is:1051-1980
SPECIFICATION
(Second Revision)
ex:i.:;i:!
t~i:
[Page
No.
l]
clause:
clause
A-4.1
(see also
9,
Substitute the following for the
Amectl!nr,i1
ex.i::t.i!-I!;
(AFCDC 6)
___-_
____
_.. _ ..-.
AMENDMENT
IS 1051: 1980
NO. 3
TO
SPECIFICATION
EXTRACTS
(Second
(Page 6, chrse
4.1 ) -
Substitute
MAY 1994
FOR PYRETHRUM
Revision)
the following for the existing:
Reprography
AMENDMENT
mi
]~i]ter Ccl.
(FAD1)
India
I
. .
\,
..
-., .
-,
-------
,8
,. . ..
AMENDMENT
Revision)
(Page 5, clause 2.4.2) Insert the following new clause after 2.4.2 and
renumber the existing clause 2.5 as 2.6:
2.5 Saponification Value When determined by the methocl ~pecified in 15 of
IS 548 (Part 1) : 1964, the Saponification value of Pyrethrutll extract shall not
exceed 10.
(FAD 1)
1
.
--
APPENDIX D
(Clause 2.2)
DETERMINATION OF COLOUR
D-0 PRINCIPLE
The natural colouring matter content of pyrethrum extract is extracted with mineral turpentine oil. The
absorbance of the solution obtained is measured by using a UV-Visible Spectrometer at a wavelength of
435 nm.
D-1 APPARATUS
D-1.1 UV-Visible Spectrophotometer suitable for measuring at wavelength of 435 nm and having a band
width of 2 nm.
D-1.2 Volumetric Flask, 20 ml capacity.
D-1.3 Measuring Cylinder
D-1.4 Cuvettes
D-2 REAGENTS
D-2.1 Solvent, mineral turpentine oil of known purity.
D-3 PROCEDURE
Take 1 ml of the pyrethrum extract in the 20-ml volumetric flask. Add to it mineral turpentine oil and make
up the volume up to the mark with mineral turpentine oil. Shake the flask to mix the contents thoroughly.
Take a pair of clean cuvettes. Switch on the Spectrophotometer and set the wavelength at 435 nm. In one
cuvette take the solvent and place this cuvette in the reference cell of the spectrophotometer. Take the
diluted sample from the volumetric flask in the other cuvette and place the cuvette in the sample cell of the
spectrophotometer. Close the chamber and measure the absorbance at 435 nm.
(FAD 1)
Reprography Unit, BIS, New Delhi, India