MCB101IntroductoryMicrobiologyLab

PracticeExam2Fall2013
ANNOUNCEMENT
Exam2forMCB101willbegivenat99:50AM,Friday,Nov.8,2013
Place:180BEV(sectionsA,B,C,D)100GH(sectionsE,F,G,H)
Examcoversexperiments914.
Ifyouneedtotaketheconflictexam,contactDr.Chapmannolaterthan5PM
Tuesday,Nov.5.Theconflictexamwillbegivenat5PMonThursday,11/7/13,in
room242BurrillHall.
PRACTICEEXAM
Foreachquestionchoosetheonebestanswer.
MultipleChoice
1)Whichoneofthefollowingstatementsaboutcarbohydratefermentationtestsisfalse?
A.Bacteriathatobtainenergybybreakingdownaminoacidsexcretebasicwastes.
B.Bacteriathatgrowbyfermentingasugarusuallyexcretesomeacidicwasteproduct.
C.InMCB101,thecarbohydratefermentationbrothweusecontainsaDurhamtube.
D.InMCB101,thecarbohydratefermentationbrothweusecontainsthepHindicator
dyebromthymolblue.
E.Ifthebacteriumcannotusethesugarinthecarbohydratefermentationbroth,they
cantgrowinthatmedium.
2)Whatsubstance(s)inlactosefermentationbrothcanactasacarbonsource?
A.Theonlysourceforcarboninlactosefermentationbrothislactose.
B.Therearetwocarbonsourcesinlactosefermentationbroth,lactoseandglucose.
C.Therearetwocarbonsourcesinlactosefermentationbroth,lactoseandaminoacids.
D.Therearethreecarbonsourcesinthisbroth,lactose,ammoniumsulfateandvitamins.
E.Thecarbonsourceinlactosefermentationbrothiscarbondioxide.
3)Whichoneofthefollowingstatementsbestdescribestheappearanceofareliable
positiveresultforacarbohydratefermentationtest.
A.Themediumhasturnedred.
B.Themediumiscloudyandyellow.
C.Themediumisclearanddoesnotchangecolor.
D.Themediumiscloudyandgreenishblue.
E.NobubbleappearsintheDurhamtube.
4)Apositiveresultforthecatalasetestistheappearanceofbubblesofoxygenwhena
reagentisplacedonthebacterialsmear.Whatisthereagentusedinthecatalasetest?


A.methylredB.hydrogensulfideC.Kovacsreagent
D.hydrogenperoxideE.acidifiedironchloride
5)Inanybacterialidentificationscheme,whichoneofthefollowingproceduresmust
bedonebeforedoingbiochemicaltests?
A.PrepareaGramstainofthebacteria.
B.Obtainapurecultureofthebacteria.
C.IncubateaplateofLagarat37degreesC.
D.Examinethebacteriawithamicroscope.
E.GrowthebacteriaonPEAagar.
6)Kliglersironagarisusedtotestforthepresenceoftheenzymecysteine
desulfhydrase,whichcatalyzesthebreakdownofcysteine.Whatproductofthisreaction
reactswiththeironthatisintheagar,toproducetheblackprecipitatethatisthesignofa
positivetest?
A.ammoniaB.pyruvateC.hydrogensulfide
D.variousacidsE.carbondioxide
7)Oneofthebiochemicaltestsweusedtoidentifybacteriachecksforthesynthesisof
theenzymephenylalaninedeaminase.Thisenzymecausesphenylalaninetobreakdown
toformphenylpyruvate+_______________.
A.ammoniaB.pyruvateC.hydrogensulfide
D.variousacidsE.carbondioxide
8)Whentheaminoacidlysineisbrokendownbylysinedecarboxylase,
______________isformedandthemediumturns______________.
A.cadaverine,purpleB.putresine,yellowC.citrate,green
D.ammonia,blueE.23butanediol,doesnotchange
9)TheMethylRedtestisusedtocheckfortheproductionof:
A.tryptophanase.B.acetoinand2,3butanediolfromglucose.
C.citricacid.D.largequantitiesofacidfromfermentationofglucose.
E.starch.
10)Indoleismadefrom:
A.asugar,lactose.B.anaminoacid,phenylalanine.
C.anaminoacid,tryptophan.D.anaminoacid,lysine.
D.aKrebscycleintermediate,citrate.

11)TheVoguesProskaurtestusesthesametypeofmediumasthe__________test.
A.indoleB.methylredC.lysinedecarboxylase
D.phenylalaninedeaminaseE.amylase
12)TheVogesProskauertestshowsifabacteriumcanproduce_____________.
A.pyruvatefromcysteineB.amixoforganicacidsfromglucose
C.cadaverineD.phenylpyruvatefromphenylalanine
E.2,3butanediolasafermentationwasteproduct
13) Bacteria in the genus Staphylococcus are Gram positive spheres or cocci.
Bacteria in the genus Streptococcus are Gram positive cocci too.
Which one of the following biochemical tests is the best way to tell the
difference between Streptococcus and Staphylococcus?
A. phenylalanine deaminase test
C. hydrogen sulfide production
E. indole test

B. methyl red test

D. catalase test

14) Serial Dilution: The Final Dilution Factor

In a three step serial dilution:
the first dilution is 1/10,
the second dilution is 1/5,
the third dilution is 1/4.
What is the total dilution factor of this three step serial dilution procedure?
A. 0.05

B. 0.005

C. 1/19

D. 19

E. 200

15)Whichoneofthefollowingbacteriaisthebestfittothisdescription?
ItispurpleintheGramstain.Thecellsareshapedlikelittleroundballs.
Thecellsarearrangedinchains.Itisnegativeforthecatalasetest.
The normal habitat for this bacterium is the mammalian large intestine.
A. Bacillus subtilis
B. Enterococcus faecium
D. Pseudomonas aeruginosa
E. Staphylococcus aureus

C. Escherichia coli

16)Ifaviableplatecountofa0.1mlsampleyields210colonies
andthedilutionfactorwas104,
whatwastheconcentrationofcellsintheoriginalsample?
A.2.1x104cells/mlB.2.1x106cells/mlC.2.1x107cells/ml
D.210x104cells/mlE.210,000cells/ml

17)Abacterialcultureisstartedwith60,000cellsandisallowedtogrowfor30
generations.Determinethefinalnumberofcellsintheculture.
A.1.80x105B.6.44x1013C.6.00x1034
D.1.20x1035E.6.44x1036
Questions18and19refertotheinformationgiveninthistable.

18)Youareattemptingtoidentifyanunknownentericbacteriumthatisoneofthe12
speciesshowninthebiochemicaltestkeythatisgivenabove.Yourunknownbacterium
ispositivefortheVogesProskauertestandpositiveforlactosefermentation.Whichone
ofthefollowingtestswouldallowyoutounambiguouslydeterminetheidentityofthe
unknown?
A.arabinosefermentationB.indoletestC.ureasetest
D.dulcitolfermentationE.esculinhydrolysis
19)Youareattemptingtoidentifyanunknownentericbacteriumthatisoneofthe12
speciesshowninthebiochemicaltestkeythatisgivenabove.Yourunknownbacterium
ispositivefordulcitolfermentation,negativeforraffinosefermentationandpositivefor
Simmonscitrate.
Whichoneofthefollowingwouldyouexpecttobetrue?

A.Yourunknownispositivefortheindoletest.
B.YourunknownproducesablackprecipitateinKliglersironagar.
C.YourunknownisCitrobacterfreundii.
D.Yourunknownispositiveforlactosefermentation.
E.Yourunknowncancreateaclearzonearoundtheareaofgrowthoneggyolkagar.

20)Whatisthegenerationtimeforthebacteriadepictedinthisgrowthcurve?

A.30minutesB.60minutesC.90minutesD.5.5hoursE.7hours
21)Forthegrowthcurvedepictedbelow,thebacterialculturecalled________grows
thefastestwhilethebacterialculturecalled________showsthegreatestnumber
ofdoublingsduringtheexperiment.

A.P,PB.P,SC.S,SD.S,P

22) Which one of the following statement about bacterial growth in broth culture is false?
A. After inoculating the broth, there may be a delay before you begin to see growth.
B. Calculations of the growth rate require data from the exponential phase.
C. A shorter generation time equals a faster growth rate.
D. The generation time is the time it takes for the bacterial population to double.
E. The bacterial population is at its maximum density during the middle of the log phase.
23) What is diaminopimelic acid?
A.
B.
C.
D.
E.

an antibiotic that inhibits bacterial cell wall biosynthesis

an antibiotic that inhibits bacterial ribosomes
an unusual amino acid found in the peptidoglycan of some Gram negative bacteria
a bright red pigment that is seen in Serratia marcescens
a vitamin that is found in L-agar

24) In a pRL27 transformation experiment such as the one we performed in MCB101, a

student got the following results:
colonies on L-agar + kanamycin: at a dilution of 10-1 , the number of colonies = 100
colonies on L-agar: at a dilution of 10-7 , the number of colonies = 50
Given the above data, what is the transformation efficiency?
A. 2 x 10-5

B. 2 x 10-6

C. 2 x 10-8

D. 5 x 10-5

E. 5 x 105

25) Which one of the following statements about a pRL27 transformation experiment
such as the one we did in MCB101 is false?
A. The plasmid pRL27 cant be replicated in many strains of bacteria, including Serratia
marcescens DI.
B. The plasmid pRL27 contains a gene that makes the host cell resistant to kanamycin.
C. The antibiotic resistance gene on pRL27 is located on a transposable element.
D. The recipient strain gains the plasmid pRL27 and becomes a donor strain that can
pass the plasmid on to other cells by conjugation.
E. The strain that acts as a donor for the plasmid pRL27 is an auxotrophic mutant of
Esherichia coli.
For each of the following tests describe: the purpose of the test, reagents used, and the
appearance of a positive and negative result.
Gram Stain
Catalase Test
Methyl Red
Indole Test

Lactose Fermentation Test

Lysine Decarboxylase
Simmons Citrate Agar
Phenylalanine Deaminase Test

O-F Glucose Test

Voges-Proskauer Test
Kliglers Test
Urease Test

MCB101MicrobiologyExam2AnswerKey
1)__E___8)___A___15)___B___22)___E__

2)__C___9)___D___16)___C___23)___C__

3)__B___10)___C___17)___B___24)___B__

4)__D___11)___B___18)___C___25)___D__

5)__B___12)___E___19)___B___

6)__C___13)___D___20)___C___

7)__A___14)___E___21)___B___