I.

Procedure
1. Making Emulsion Solution (Mobility Phase)
25mL of n-butanol + 6mL of
acetic acid glacial + 25mL aquades
Poured into chamber
Mixture and shaked
Allowed with vapor
Eluent ready to use

2. Determining the component of amino acid

Chromatography paper 4 x 10 cm

Given the limit over 1 cm, the limit upper 0.5 cm

Given the limit for a sample and standart
Oven 5-10 menits in 100C-110C
Chromatography paper
Sample solution S1, S2, and standard solution

Dropped on the chromatography paper

Drained
Eluent from the chamber
Marked the movement of the end of eluen
Oven 5 minutes at 106C
Sprayed with ninhydrin
Roasted again 5 minutes marked trains that
appears with a pencil
Plaster with a duct tape
Calculated Rf the sample and standard
solution and compare it

Value of Rf