Professional Documents
Culture Documents
Computational Biochemistry
ENZYPLOT: a microcomputer assisted program for teaching enzyme
kinetics, F. Leone et al., Biochem. Educ. 23, 35-37 (1995).
Molecular modeling and experimental verification of the relative stability of
DNA base pairs: a biochemistry laboratory experiment, S. Burgess et al.,
Biochem. Educ. 24, 230-231 (1996).
Structural analysis and modeling of proteins on the web, D. Leon, S.
Uridil, and J. Miranda, J. Chem. Educ. 75, 731-734 (1998).
Teaching Bioinformatics, H. Salter, Biochem. Educ. 26, 3-10 (1998).
Using the computer in biochemical research, R. Boyer, Modern Experimental
Biochemistry, 3rd ed., p. 211-225 (2000), Benjamin Cummings (San Francisco).
Integrating computational chemistry into a project-oriented biochemistry
laboratory experience: a new twist on the lysozyme experiment. R. Peterson,
J. Cox, J. Chem. Educ. 78, 1551-1555 (2001).
Integrating Internet assignments into a Biochemistry/Molecular Biology
laboratory course, R. Kaspar, Biochem. Mol. Biol. Educ. 30, 36-39 (2002).
Introduction to interpretation of stochastic parameters: computer-intensive
procedures for evaluation of data in enzyme kinetics, K. Tenekedjiev and
K. Kolev, Biochem. Mol. Biol. Educ. 30, 414-418 (2002).
BioMoleculesAlive.org: The biochemistry and molecular biology digital
library update, P. Craig, Biochem. Mol. Biol. Educ. 31, 151-152 (2003).
Photochemical oxidation of bilirubin to biliverdin, W. Coleman, J. Chem.
Educ. 83, 1329-1330 (2006).
Utilization of integrated Michaelis-Menten equation to determine kinetic
constants, R. Bezerra and A. Dias, Biochem. Mol. Biol. Educ. 35, 145-150
(2007).
Molecular models of DNA, W. Coleman, J. Chem. Educ. 84, 809-810
(2007).
Molecular models of resveratrol, W. Coleman, J. Chem. Educ. 84, 11611162 (2007).
Lysozyme thermal denaturation and self-interaction, J. Schwinefus et al.,
J. Chem. Educ. 85, 117-120 (2008).
Electrophoresis
SDS-agarose gel electrophoresis in a simple procedure for determining high
molecular weight protein oligomerization, M. Brownleader et al., Biochem.
Educ. 22, 155-158 (1994).
Capillary electrophoresis: a fast and simple method for the determination of
the amino acid composition of proteins, P. Weber and D. Buck, J. Chem.
Educ. 71, 609-611 (1994).
An inexpensive agarose gel electrophoresis system, D. Betsch and J. Beard,
Biochem. Educ. 27, 48-50 (1999).
Electrophoretic transfer technique to detect and identify amylases in
polyacrylamide gels, V. Mulimani and S. Thippeswamy, Biochem. Mol.
Biol. Educ. 29, 250-254 (2001).
Determination of a diffusion coefficient by capillary electrophoresis, K.
Williams, B. Adhyaru, I. German, and T. Russell, J. Chem. Educ. 79, 14751476 (2002).
Agarose gel electrophoresis system in the classroom. J. De Mattos et al.
Biochem. Mol. Biol. Educ. 32, 254-257 (2004).
An SDS-PAGE examination of protein quaternary structure. J. Powers et al.
J. Chem. Educ. 82, 93-95 (2005).
Determination of the subunit molecular mass and composition of alcohol
dehydrogenase by SDS-PAGE, B. Nash, J. Chem. Educ. 84, 1508-1511
(2007).
Metal-catalyzed cleavage of tRNA-Phe, S. Kirk et al., J. Chem. Educ. 85,
676-678 (2008).
Automated data collection from manual Hitachi model U-1100 spectrophotometers and analysis by means of Excel spreadsheets, D. Titheridge et al.,
Biochem. Educ. 23, 91-96 (1995).
Concepts and skills in the biochemistry/molecular biology lab, R. Boyer,
Biochem. Mol. Biol. Educ. 31, 102-105 (2003).
Teaching undergraduates to write scientific papers, L. Tomaska, ASBMB
Today, May, 20-21 (2007).
Ligand Binding
Spectrophotometric determination of the binding constants of succinate and
chloride to glutamic oxalacetic transaminase, A. Parody-Morreale et al., J.
Chem. Educ. 67, 988-991 (1990).
The binding of coomassie brilliant blue to bovine serum albumin, J. Sohl
and A. Splittgerber, J. Chem. Educ. 68, 262-264 (1991).
Evaluation of the Hill coefficient from Scatchard and Klotz plots, A. Sabouri
and A. Moosavi-Movahedi, Biochem. Educ. 22, 48-49 (1994).
Fluorescence measurements of ethidium binding to DNA, K. Strothkamp
and R. Strothkamp, J. Chem. Educ. 71, 77-81 (1994).
Analysis of receptor-ligand interactions, A. Attie and R. Raines, J. Chem.
Educ. 72, 119-121 (1995).
A ligand-binding study based on a protein assay method, H. Chial et al., J.
Chem. Educ. 72, 76-78 (1995).
Binding of bilirubin to goat serum albumin: determination of binding
constants, S. Tayyab and V. Trivedi, Biochem. Educ. 23, 98-100 (1995).
The shapes of Scatchard plots for systems with two sets of binding sites, A.
Bordbar et al., Biochem. Educ. 24, 172-175 (1996).
Fluorescent measurement of TNS binding to calmodulin, J. Buccigross et
al., J. Chem. Educ. 73, 275-278 (1996).
Binding of proflavin to chymotrypsin, H. Hendrickson, Biochem. Educ.
27, 118-121 (1999).
Study of protein-ligand Binding by fluorescence, M. Moller and A.
Denicola, Biochem. Mol. Biol. Educ., 30, 309-312 (2002).
Teaching receptor theory to biochemistry undergraduates, M. BenoreParsons and K. Sufka, Biochem. Mol. Biol. Educ. 31, 85-92 (2003).
Equilibrium gel filtration chromatography for the measurement of proteinligand binding in the undergraduate biochemistry laboratory. D. Craig, J. Chem.
Educ. 82, 96-98 (2005).
Quantitative determination of DNA-ligand binding using fluorescence
spectroscopy, E. Healy, J. Chem. Educ. 84, 1304-1307 (2007).
Molecular models of DAPI, W. Coleman, J. Chem. Educ. 84, 1307 (2007).
Steady-state fluorescence anisotropy to investigate flavonoids binding to
proteins, C. Ingersoll and C. Strollo, J. Chem. Educ. 84, 1313-1316 (2007).
UV thermal melting curves of tRNA-Phe in the presence of ligands, S. Kirk
et al., J. Chem. Educ. 85, 674-676 (2008
Spectroscopy
Fluorescence quenching of albumin. A spectrofluorimetric experiment, M.
Montero et al. Biochem Educ. 18, 99-101 (1990).
Using high field NMR to determine dehydrogenase stereospecificity with
respect to NADH, S. Mostad and A. Glasfeld, J. Chem. Educ. 70, 504-506
(1993).
NMR titration used to observe specific proton dissociation in polyprotic
tripeptides, J. Yarger, R. Nieman, and A. Bieber, J. Chem. Educ. 74, 243-245
(1997).
Resonance energy transfer in peptides: A biophysical laboratory experiment, A.
Hundzinski and B. Anderson, J. Chem. Educ. 76, 416-418(1999).
Proton NMR Studies of the conformation of an octapeptide, A. Rehart and J.
Gerig, J. Chem. Educ. 77, 892-895 (2000).
Carboxyfluorescin fluorescence experiments, S, Massou, R. Albigot, and
M. Prats, Biochem. Educ. 28, 171-173 (2000).
Protein tryptophan accessibility studied by fluorescence quenching, M.
Moller and A. Denicola, Biochem. Mol. Biol. Educ. 30, 175-178 (2002).
The free energy of denaturation of lysozyme: an undergraduate experiment
in biophysical chemistry, W. Kurtin and J. Lee, Biochem. Mol. Biol. Educ.
30, 244-247 (2002).
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Teaching protein purification and characterization techniques: A studentinitiated, project-oriented biochemistry laboratory course, G. MacDonald,
J. Chem. Educ. 85, 1250-1253 (2008).
Identifying gel-separated proteins using in-gel digestion, mass spectrometry,
and database searching, D. Beussman et al., Biochem. Mol. Biol. Educ. 37,
49-55 (2009).
Carbohydrates
Changes in carbohydrate content during fruit ripeninga new approach of
teaching carbohydrate chemistry in biochemistry course, P. Chaimanee
and O. Suntornwat, Biochem. Educ. 22, 101-102 (1994).
Guidelines for determining the glycosylation characteristics of glycoconjugates
by simple procedures, J. Cabezas, Biochem. Educ. 23, 168-170 (1995).
Change in carbohydrate content during chickpea cooking: a new approach
of teaching of carbohydrate chemistry in biochemistry course, V. Mulimani,
S. Thippeswamy and Ramalingam, Biochem. Educ. 25, 44-45 (1997).
Carbohydrate Analysis: Can we control the ripening of bananas?, S. Deal,
C. Farmer, and P. Cerpovicz, J. Chem. Educ. 79, 479-480 (2002).
Lipids
Isolation and spectrophotometric characterization of photosynthetic
pigments, R. Boyer, Biochem. Educ. 18, 203-206 (1990), and Modern
Experimental Biochemistry, 3rd ed., p. 333-344, (2000) Benjamin Cummings.
(San Francisco).
Erythrocyte storage as a model for lipid peroxidation studies. An experimental assay for students of biochemistry and molecular biology, M.
Llanillo et al., Biochem. Educ. 22, 158-161 (1994).
Purification and characterization of triacylglycerols in natural oils, R. Boyer,
Modern Experimental Biochemistry, 3rd ed. p. 303-320 (2000), Benjamin
Cummings (San Francisco).
Determination of the Fatty Acid Content of Biological Membranes: A highly
versatile GC-MS Experiment, E. Schultz and M. Pugh, J. Chem. Educ. 78,
944-946 (2001).
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An experiment on the denaturation of -chymotrypsin by an anionic surfactant, sodium dodecyl sulfate (SDS), V. Mulimani and J. Lalitha, Biochem.
Educ. 24, 52-54 (1996).
A rapid and inexpensive procedure for the determination of amylase activity,
V. Mulimani and J. Lalitha, Biochem. Educ. 24, 234-235 (1996).
Spectrofluorimetric visualization of the progress of an enzymatic reaction,
C. Saldanha and J. Martins-Silva, Biochem. Educ. 24, 235-236 (1996).
Regiospecific ester hydrolysis by orange peel esterase, T. Bugg, A. Lewin,
and E. Catlin, J. Chem. Educ. 74, 105-107 (1997).
Inhibition of cholinesterase by insecticides, L. Lai and J. Ho, Biochem. Educ.
25, 235-237 (1997).
The thermodynamic stability and catalytic activity of yeast alcohol dehydrogenase at different pH values, R. Tabor and J. Ladwig, Biochem. Educ. 25, 169170 (1997).
A simple laboratory experiment for the teaching of the assay and kinetic
characterization of enzymes, L. Vicario, D. Casti, and A. Iglesias, Biochem.
Educ. 25, 106-109 (1997).
A novel approach to practical enzymology teaching: a conductimetric
investigation of arginase, inorganic pyrophosphatase, aliphatic esterase,
ornithine carbamyl transferase, and arginosuccinate lyase activities from
mammalian liver, A. Lawrence et al., Biochem. Educ. 26, 56-62 (1998).
Immobilized lactase in the biochemistry laboratory, M. Allison and C. Bering,
J. Chem. Educ. 75, 1278-1281 (1998).
Purification of bovine carbonic anhydrase by affinity chromatography, C.
Bering and J. Kuhns, J. Chem. Educ. 75, 1021-1025 (1998).
A 19-F NMR study of enzyme activity, K. Peterman, K. Lentz, and J. Duncan,
J. Chem. Educ. 75, 1283-1285 (1998).
The competitive inhibition of yeast alcohol dehydrogenase by 2,2,2-trifluoroethanol, R. Tabor, Biochem. Educ. 26, 239-242 (1998).
Kinetics of Papain: An introductory biochemistry laboratory experiment, K.
Cornely, E. Crespo, M. Earley, R. Kloter, A. Levesque,and M. Pickering,
J. Chem. Educ. 76, 644-646 (1999).
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IV. Metabolism/Regulation/Transport
The energetics of aerobic versus anaerobic respiration, T. Champion and
R. Schwenz, J. Chem. Educ. 67, 528-530 (1990).
Use of DCPIP in a colorimetric method to investigate electron transport in
crude heart mitochondrial extracts, A. Myers, Journal of Biol. Educ. 24, 123126 (1990).
Mitochondria from rat liver: method for rapid preparation and study, C. Heisler,
Biochem. Educ. 19, 35-38 (1991).
An experiment on glycogen biosynthesis in E. coli, A. Lodeiro et al, Biochem.
Educ. 22, 213-214 (1994).
Demonstration of the regulation of phosphorylase by reversible
phosphorylation and allosteric effectors, R. Toomik et al., Biochem. Educ. 22,
205-207 (1994).
An experiment illustrating catabolite repression in yeast, W. Baker, Biochem
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V. Clinical/Nutritional
Determination of urinary ammonium: a practical class experiment designed
to introduce medical students to mechanisms of extracellular and intracellular pH homeostasis, C. Marinho and J. Barros, Biochem. Educ. 22,
215-216 (1994).
A practical approach to chemical sensors through potentiometric transducers:
determination of urea in serum by means of a biosensor, E. Martinez and
S. Alegret, J. Chem. Educ. 71, A67 (1994).
The role of molecular biology in characterization of -thalassaemia,
J. Howarth et al., Biochem. Educ. 24, 59-61 (1996).
Use of blood-glucose test strips for introducing enzyme electrodes and modern
biosensors, J. Wang and C. Macca, J. Chem. Educ. 73, 794-797 (1996).
The analysis of riboflavin in urine using fluorescence, J. Henderleiter and R.
Hyslop, J. Chem. Educ. 73, 563-565 (1996).
Working with enzymeswhere is lactose digested?, S. Pope, T. Tolleson,
R. Williams, R. Underhill, and R. Deal, J. Chem. Educ. 75, 761-762 (1998).
Measurement of cholesterol and vitamin C in biological samples, R. Boyer,
Modern Experimental Biochemistry, 3rd ed. p. 371-388 (2000), Benjamin
Cummings (San Francisco).
An experiment on the apoptosis induced by polyamine adducts produced
in the presence of serum amine oxidase, I. Fajardo, Biochem. Educ. 28,
110-112 (2000).
Glucocorticoid-induced apoptosis: a simple set of laboratory experiments,
G. Vicent, G. Lanuza, D. Romero, L. Di Croce, and A. Pecci, Biochem. Mol.
Biol. Educ. 28, 307-312 (2000).
From gene to protein: Prostatic acid phosphatase: Structure and expression of
gene and protein. P. Laidler et al., Biochem. Mol. Biol. Educ. 32: 400-409 (2004).
Testing for genetically modified foods using PCR. A. Taylor and S. Sajan, J. Chem.
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biology laboratory, M. Black et al., Biochem. Mol. Biol. Educ. 36, 217-224 (2008).
Probing changes in the conformation of tRNA-Phe: an integrated biochemistry
laboratory course, S. Kirk et al., J. Chem. Educ. 85, 666-674 (2008).
Teaching protein purification and characterization techniques: A studentinitiated, project-oriented biochemistry laboratory course, G. MacDonald,
J. Chem. Educ. 85, 1250-1253 (2008).
Thermal and chemical denaturation of Bacillus circulans xylanase, L. Gentile et
al., Biochem. Mol. Biol. Educ. 36, 428-432 (2008).
Vertical and horizontal integration of bioinformatics education, L. Furge et al.,
Biochem. Mol. Biol. Educ. 37, 26-36 (2009).
RNase One gene isolation, expression, and affinity purification, C. Bailey,
Biochem. Mol. Biol. Educ. 37, 44-48 (2009).
From gene mutation to protein characterization, D. Moffet, Biochem. Mol. Biol.
Educ. 37, 110-115. (2009).
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