OBSERVATION RESULT

No
1.

Prosedures
Treatment step of sampel

Observation Result
Before
- Coconut oil : yellow solution
After
- Heating : yellow (++)
solution

Hypothesis/reaction
Conclusion
CH3(CH2)14COOH + KI + If coconut oil is
H2O CH3(CH2)14COOH heated will form fatty.
25 ml of sample
+ I2 + 2 KOH

I2 + 2 S2O32- 2 I- +
Boiled with heating time of 15, 30, 45, 60 m
S4O62Left in opened place
IO3 + 5I +6H+ 3I2 +
H2O

2.

Determination of peroxide number

Before
Glacial acetic acid : colorless
solution
Chloroform
:
colorless
solution
KI : yellow solution
After
Coconut oil : glacial acetic
acid : colorless solution
+ chloroform : colorless
solution
+ KI : yellow solution
+aquades : turbid yellowish
solution
+ starch solution : Blackish
yellow solution
Titrated with Na2S2O3 0.1 N
: form two layers (upper :

sample

The longer heating the

greater
peroxide
Amylum +
iod2 of
20nI
drops
sample
from
each
heating proc
number.
amylum complex
Order of rancidity
Rancidity reaction :
reaction is second
ml of glacial acetic acid
R CH = CH R+ Added
O = O 3,6
order.
Added 2,4 ml of chloroform

Added 2 drops of saturated KI

HC R
R CHWaited for 1 minutes while shaked s
||
||
Added 6 ml of aquades
O
O
Added 2 drops of starch solution1 %
Titrated with Na2S2O3 0,1 M

oil = 0.903 g/ml

Reaction of starch:

(Ditjen POM, 1979)

Volume of titration

colorless; bottom : oily and

white)
- Volume:
V 15 m : 0.61 ml
V 30 m : 0.84 ml
V 45 m : 1.4 ml
V 60 m : 1.87 ml
V 2 h : 1.93 ml

3.

Titration of blanco solution

Before
- Glacial acetic acid : colorless
solution
- Chloroform
:
colorless
solution
- KI : yellow solution
After
- glacial
acetic
acid
+
chloroform
:
colorless
solution
- + KI : soft yellow (-) solution
- +aquades : turbid solution
- + starch solution : yellow
(--) solution
- Titrated with Na2S2O3 0.1 N
: yellowish solution
- Volume : V1: 0.48 ml ; V2 :
0.54 ml ; V3 = 0.5 ml
V average = 0.51 ml

CH3(CH2)14COOH + KI +
The acid+2,4
function mlofof chlorofo
of glacial acetic
H2O 3,6
CHml
3(CH2)14COOH
blanco solution as
+ I2 + 2 KOH
standard solution.
I2 + 2 S2O32- 2 I- +
Added 2 drops of saturated KI
S4O62Waited for 1 minutes while shaked slowl
+
IO3 + 5I +6HAdded
3I26 +ml of aquades
Added 2 drops of starch solution1 %
H2O
Titrated with Na2S2O3 0,1 M
Amylum + nI2 iodamylum complex
Volume of titration

PROCEDURES
1. Treatment step of sampel
25 ml of sample
Boiled with heating time of 15, 30, 45, 60 m and 2 hours.
Left in opened place
sample

2. Determination of peroxide number

20 drops of sample from each heating process

Added 3,6 ml of glacial acetic acid

Added 2,4 ml of chloroform
Added 2 drops of saturated KI
Waited for 1 minutes while shaked slowly
Added 6 ml of aquades
Added 2 drops of starch solution1 %
Titrated with Na2S2O3 0,1 M

Volume of titration
3. Titration of blanco solution
3,6 ml of glacial acetic acid+2,4 ml of chloroform

Added 2 drops of saturated KI

Waited for 1 minutes while shaked slowly
Added 6 ml of aquades
Added 2 drops of starch solution1 %
Titrated with Na2S2O3 0,1 M

Volume of titration