LAB INFECTIOUS

DISEASE

388-421, 388-422,388-423
1, 2, 3
Health and Diseases of Adults and Elderly I, II, III
2555

The M.D. Program Prince of Songkla University (254 credits)

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1, 2, 3 (Health
and Diseases of Adult and Elderly)
slide


slide

lab ID Interpretation



verify

(Fresh preparation)
(staining)
smear (gram stain)
Gram Stain
Acid fast bacilli (AFB) Kinyoun technique
Modified AFB

1
2
5
7
9
11


(Fresh preparation)
(specimen)
mycelium mycelium Aspergillus spp.
Gram
stain
yeast
cell,
pseudomycelium chlamydospore


polar flagella (shooting)

1) Indian
ink
Cryptococcus neoformans CSF
2) 10% KOH
Sputum, cervix discharge
: Indian ink 10% KOH cover glass
10x, 40x
condenser condenser diaphragm

 (staining)



slide :
specimen
1. hemoculture
(sterile)

hemoculture incubate 24 smear

fix iodine acetone
30 (gram stain)
100x oil

2. (CSF) (sterile fluid)
CSF body fluid normal flora
(WBC count) WBC 3002,000 cell/ml
CSF body fluid
slide fix fix acid
alcohol slide acid alcohol
slide
2

3. (sputum)
swab

smear 2
- hood

(Concentration)
5% Sodium hypochlorite
(Clorox)R 2-3
- 3,000 / 10
- smears
acid-fast
(true sputum)
40x WBC >25 cells, epithelial cell <
10 cells
4. (PUS)
pus smear slide



anaerobe

5. (Urine)
- 40x
>20 cells/field 1 cell/field

- smear slide fix

acid alcohol ()

 smear (gram stain)

1. smear
slide, smear

2. objective eyepiece
condenser
3. smear low power objective len
(10x) smear
3.1 polymorphonuclear leukocytes
acute bacterial infection
leukocytes
3.2 WBC epithelial cell
specimen
3.3
inflammatory cell iris diaphragm
4. oil smear oil immersion
objective oil oil
smear smear
smear
5. (artifact)
- cocci
-

6.

7. gram positive cocci
pneumococci

8. : numerical

1+
2+
3+
4+

field (oil 100x)

<1
1
2-10
> 10

Slide
- Slide xylene
- smear decolorize decolorize
alcohol safranin
- smear decolonize

 Gram Stain
1.
- body fluid , smear
film
- , , specimen

smear film
2.
3. Fix slide slide
2-3 (Heat fix)

4. crystral violet 1

5. 1
6. 95 % Ethanal

7. safranin 30

8. oil 1

 1 gram stain

 Acid fast bacilli (AFB) Kinyoun technique

1. kinyoun carbol fuchsin solution

2. 3 % Acid alcohol
3. 0.1 % Methylene Blue

1. slide specimens slide

2. slide carbol fuchsin slide 5
3.
4. 3 % Acid alcohol
5.
6. 0.1 % Methylene Blue 1
7. slide

Acid fast
carbol fuchsin

decolorizer, acid alcohol

methelene blue

2 AFB

10

non Acid fast

 Modified AFB
Modified AFB stain
Nocardia spp. , Rhodococcus equi, Cryptosporidium
Kinyoun 1% H2SO4 acid alcohol
Nocardia spp. partial acid fast positive
branching filaments

1) kinyoun carbol fuchsin solution

2) 1% sulfuric acid
3) 0.1 % methylene blue

1. slide specimens slide

2. slide kinyoun carbol fuchsin solution slide 5
3.
4. 1% Sulfuric acid
5.
6. 0.1 % Methylene Blue 1
7. slide

Positive : partial acid fast positive branching filaments

Negative : partial acid fast positive branching filaments
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