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Answer
20 minutes.
4,300
Solid tissues in animals include epithelial tissue, _______ tissue, nervous tissue, and
muscle.
connective
In fluorescence microscopy, the immediate source of the light detected is light that has been
emitted by
_______ the sample.
The feature that most clearly separates eukaryotes from prokaryotes is the presence of
_______ in eukaryotic cells.
a nucleus
What is the theoretical diffraction limit of resolution of a light microscope used to look at a
sample through oil?
0.22 m
What is the smallest number of ultracentrifugation steps needed to separate nuclei from
ribosomes in a cellular lysate?
In contrast to animal cells, yeast or bacterial cell cultures can be grown on fairly simple
media without the need to add amino acids or various vitamins and hormones. This is
primarily because animal cells
Which of the following are the most commonly used mammals for genetic studies?
Mice
Archaebacteria
The original cell was thought to have arisen from the enclosure of self-replicating _______
by a phospholipid membrane.
RNA
The source of the atmospheric oxygen necessary for the development of oxidative
metabolism is thought to have been
photosynthesis.
Approximately how many doublings can normal human fibroblasts undergo in culture?
50 to 100
Embryonic stem cells are different from primary cell cultures or permanent cell lines
Bacteriophages are
amphipathic.
Proteins
Chymotrypsin, trypsin, elastase, and thrombin are all grouped together as members of the
serine protease family because each
Which of the following classes of amino acids is buried within the folded structure of the
protein?
Nonpolar
Phospholipids consist of a 3-carbon core to which fatty acids and a phosphate group are
linked. The most common 3-carbon core is
glycerol.
Enzymes act by
Which of the following is not one of the four major classes of organic molecules in the cell? Water
vitamins.
All of the following are ways in which enzyme activity can be regulated except
It increases permeability
Starch
Proteins must have more than one _______ to have a quaternary structure.
polypeptide chain
water
Introducing a double bond into a fatty acid has the effect of putting a(n) _______ into the
conformation of the molecule.
kink
20
X-ray crystallography.
About 50% of the mass of most biological membranes consists of lipids and about 50%
consists of proteins. Therefore,
estradiol
cysteine
Secondary
In the fluid mosaic model of biological membrane structure, transmembrane proteins are
The end product of the anaerobic metabolism of glucose may be any of the following except methanol.
chlorophyll
Which of the following is a key molecule in the electron transport chain in mitochondria?
Cytochrome c
chlorophyll.
Animal cells can synthesize glucose (gluconeogenesis) from all of the following except
fatty acids.
The ATP synthases of mitochondria and chloroplasts are examples of _______ proteins in
which polypeptide rotation provides a mechanical coupling to ATP synthesis.
motor
What is the major site of energy production in the form of ATP in human cells?
Because fatty acids are synthesized by the stepwise addition of acetyl CoA derivatives, they
2
differ in length by units of _______ carbon(s).
Antimetabolite chemotherapy succeeds as a treatment for childhood leukemia because
Which statement correctly describes the difference between the proton gradient across the
inner mitochondrial membrane and the proton gradient across the thylakoid membrane of
chloroplasts?
What is the change in free energy (G) for the hydrolysis of ATP to ADP and Pi in the cell?
12 kcal/mol
Together, glycolysis and the citric acid cycle directly generate two molecules of ATP and
two molecules of GTP. Most of the energy derived from the oxidation of glucose comes
from the oxidation of the _______ generated from _______.
ammonia
What are the energetic consequences of coupling a reaction with a +G to one with a larger
G?
Nucleotide chains are elongated in the 5-to-3 direction. The 5 and 3 refer to the chemical
positions on the
ribose or deoxyribose.
UDP-glucose.
The electrochemical gradient across the mitochondrial inner membrane drives the import of
cytochrome c.
all of the following except
Light is captured by _______ different photosystems associated with the thylakoid
membranes of chloroplasts.
two
How many carbons does the acetate group of acetyl CoA have?
What is the net yield of ATP molecules per molecule of glucose from the process of
glycolysis?
Which of the following techniques is used to inactivate a gene by altering the DNA that
encodes it?
Homologous recombination
The CRISPR/Cas system is a fast and powerful method to introduce mutations into cellular
Mutant copy of target gene, guide RNA,
genomes via homologous recombination to study the function of genes and the proteins they
and Cas9
encode. Which of the following are necessary reagents for CRISPR/Cas?
Which of the following terms refers to a collection of clones that contain all the genomic or
mRNA sequences of a particular cell type?
reverse transcriptase.
Which of the following is not necessary in a plasmid vector to facilitate successful cloning?
What technique would you use to determine if the level of a specific mRNA had been
increased in response to an inducer?
Northern blotting
The two strands of DNA in the double helix are held together by
A restriction enzyme with a four-base recognition site would cleave DNA with a statistical
frequency of once every
The process by which proteins are made from RNA templates is called
translation.
Which of the following serves as an adaptor between amino acids and mRNA during
translation?
tRNA
Which of the following correctly represents the distinction between the terms genotype
and phenotype?
All of the following are examples of cloning vectors except a. plasmids. b. cosmids. c. yeast
bacteriophages.
artificial chromosomes (YACs). d. bacteriophages.
Transgenic mice carry a foreign gene
How many molecules would be produced from two molecules of DNA following five
rounds of PCR amplification?
64
Bioinformatics is
The eukaryote Caenorhabditis elegans has been used especially in the study of
animal development.
Which of the following organisms has the highest percentage of its genome used to encode
protein?
Haemophilus influenza
A collection of all the RNAs that are expressed at any given time in a cell are referred to as
the
transcriptome.
The main difference between the genomes of the bacteria Haemophilus influenzae and E.
coli is that
Humans have about 21,000 genes that encode proteins. It is estimated that due to alternative
splicing and protein modifications, these genes can give rise to how many different
100,000
proteins?
Which of the following methods can accurately quantify the amount of mRNA expressed in
DNA microarray and RNA-seq
a given cell or tissue sample?
An estimated 40% of the human genome contains proteins related to proteins that are also
present in a diverse number of other species. These proteins are involved in which of the
following cellular processes?
a. Replication b. Transcription c.
Translation
All of the following are methods that allow scientists to investigate protein-protein
interactions. Which method utilizes gene expression as the detection component?
Yeast two-hybrid
Neandertals are thought to be our closest evolutionary relatives, having diverged from
humans 300,000 to 400,000 years ago, and their genome has been sequenced. Which of the
following statements about humans and Neandertals is false?
Experimentally, you discover that in addition to stimulating the G protein, the epinephrine
receptor can also directly stimulate glycogen synthase, bypassing the intermediate proteins.
This would be an example of
a feedforward relay.
The plant Arabidopsis thaliana contains significantly more genes than either C. elegans or
Drosophila. Which of the following statements is true?
Arabidopsis genome
Two-dimensional gel electrophoresis is not an acceptable method to protein-protein
interactions. Which of the following statements best describe the reason why?
In RNAi screens, siRNAs are incubated with cells in culture to assess functional
consequences of the targeted gene. Which of the following statements is true in regards to
the siRNA and its action?
In exerting its biological activity on cells, epi binds to a cell surface receptor, which
activates a G protein, which then activates cAMP-dependent protein kinase, then phos
kinase, then gly synthase, which then breaks down glu. This is an example of
a signaling pathway
Which of the following molecular biology methods would best be used to directly determine
DNA microarray
the relative level of all mRNAs in a given cell or tissue?
Which of the following statements about exons is false?
Extensive digestion of chromatin with micrococcal nuclease was found to yield particles
called nucleosome core particles that appear as beads when viewed by electron microscopy. One molecule of H1 protein
Which of the following is not found in the nucleosome core particle?
The ENCODE project revealed that as much as 75% of the genome in humans is actually
transcribed into RNA. Most of this is non-protein coding RNA. Which of the following
include two new classes of noncoding RNA discovered by the ENCODE project?
Partial digestion of chromatin with micrococcal nuclease was found to yield DNA
fragments approximately 200 base pairs long. What accounts for this phenomenon?
The human genome contains only 20,000 to 25,000 actual gene sequences, yet the human
genome can produce up to 100,000 gene products. Which of the following processes
accounts for this difference?
Alternative splicing
The percentage of the average human gene that encodes proteins is approximately
3%
base pairs.
In dogs, the Fgf4 gene encodes a protein that regulates bone growth and in its normal
chromosomal location, Fgf4 yields dogs with long legs. Which of the following statements
best explains the mechanism leading to a short-legged phenotype in dogs?
If a human gene is found to contain five introns, the mature mRNA encoded by that gene
would have how many exons?
Which of the following statements about introns in the yeast Saccharomyces cerevisiae is
true?
Even though the complete genome is significantly smaller than humans, the flowering plant
26,000
Arabidopsis thaliana has about _______ genes, about the same number as humans have.
In the chromatin around centromeres, nucleosomes are present that have a unique structure
in which the core protein _______ is replaced by _______ and this variant of the
nucleosome is required for protein assembly at the kinetochore.
23
Which of the following transposable elements accounts for ~21% of the human genome?
Which of the following statements best describe the mechanism of action of miRNAs in
blocking expression of proteins?
A pseudogene is a
DNA methylation
E. coli preferentially utilizes glucose for energy. However, in the absence of glucose, genes
that can metabolize alternative sugars are activated. Low glucose
Which of the following best describes the ability of an enhancer to mediate transcription
from very distant sites?
RNA polymerase II
Suppose that a gene has three exons and two introns in the following order:
Which of the following both stabilizes and increases the efficiency of translation of an
mRNA?
Acidic
Northern blotting
RFC
A yeast mutant shows decreased expression of the 5.8S rRNA, the 5S rRNA, and proteinencoding mRNAs. In which of the following might the mutation lie?
Protein phosphatases
Glycosylation
A proteasome is a
The proteins shaped like a double chamber that are involved in protein folding are called
chaperonin proteins.
methionine.
minutes to days.
Antibiotics are medications that inhibit the growth of bacteria. Many target the process of
protein synthesis in the bacterial cell. In the developing of an antibiotic, which of the
following would be an effective strategy or target for the drug?
Which of the following statements about the attachment of amino acids to tRNAs is false?
a. Glycosylation b. Proteolysis c.
Palmitoylation
Signal sequences are sequences of hydrophobic amino acids that target membrane
translocation. Signal sequences are found
Which of the following processes does not require the assistance of chaperones?