6X Protein Loading Buffer

For 50ml:

30% glycerol
Stacking Buffer
6mM EDTA
10% SDS
60mM DTT
Bromphenol Blue

15ml
28ml
600l of 0.5M
5g
0.4626g
6mg

Bromphenol Blue take Sodium Salt to avoid pH-ing.

10X SDS Running Buffer

For 1liter : 30.2g Tris Base (MW 121.14)
10g SDS
(MW 288.38)
144g Glycine (MW 75.07)

Coomassie stain
45% Methanol
7.5% Acetic acid
0.1% Brilliant Blue

DestainI
30% Methanol
10% Acetic acid

Destain II
5% Methanol
7% Acetic acid
5% glycerol

6X DNA Loading Buffer

Front runs at~30bp
60mM EDTA pH8
0.3% SDS
60% glycerol
Orange G( Sigma #O3756)

6ml of 0.5M
300mg
35ml
pinch

OR:
70% glycerol
Orange G

50X TAE Buffer

For 1L : 242g Tris Base (MW 121.14)
57.1 ml glacial acetic acid
32.2g Na2EDTAx2H2O (MW 372.24)