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UNIVERSIDAD DE ZAMBOANGA

School of Allied Medicine (SAM)


Pharmacy Department

PHARM BIO SCI 3 LABORATORY


PHARMACEUTICAL BIOCHEMISTRY
Name: ___________________________ Course/Sec: ____________ Date Submitted: ________________
Group Name:______________________ Schedule: _______________ Date Performed: ________________

Activity No. 1 PHYSICAL CHEMISTRY

I.

OBJECTIVES:
1. To be able to distinguish the difference between Emulsions and Suspensoids based on their
properties:
2. To perform Dialysis and Adsorption.

II.

MATERIALS:
Test-tubes (10mL)
Test-tube Rack
Iron Stand w/Ring

III.

Bunsen Burner
Graduated Cylinder
Beaker (50mL & 250 mL)

Casserole
Droppers
Funnel

Filter paper
Pipette (10mL)
Stirring Rod

PROCEDURES:
A. COLLOIDS:
Colloidal Solutions are those in which the Diameter of the Dispersed Phase is larger than 1
millimicron but not exceeding 100 millimicra. They are classified as Emulsoids and Suspensoids.
Emulsoids have affinity for Solvent. It forms Gel when brought in contact with water. While
Suspensoids have no Affinity for Water, so they are considered As Lyophobic.

1. Preparation of an Emulsoid:
a. Prepare 5% gelatin solution by Boiling.
b. Cool under Tap Water and Note what is formed.
c. Boil again and observe the Difference in its texture when HOT and when it is COLD.

2. Preparation of a Suspensoid:
a. Boil 100 mL of Water.
b. Remove from the Flame and add 1 mL of Saturated Ferric Chloride Solution.
c. Note what is formed.
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3. Properties of Emulsoids and Suspensoids:
3.1 FOAM FORMATION:
a. Shake 10 mL of 5% gelatin vigorously with air.
b. Allow the tube to stand and note the result after 15 minutes.
c. Shake 10 mL of ferric Hydroxide in the same way.
d. Allow to stand and Note the result after 15 minutes.
e. Compare the result obtained with the Solutions.

3.2 Precipitation with Electrolytes:


a. Place 5 mL of 5% gelatin in a test tube.
b. Add Saturated Ammonium Sulfate Drop by Drop at the same time counting the number of Drops
until a Permanent Precipitate is Formed.
c. Repeat the Procedure using Ferric hydroxide and note the result.
d. Explain the Difference obtained. ___________________________________________________
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3.3 REVERSIBILITY:
a.
b.
c.
d.
e.
f.

Place 5 mL of Ferric Hydroxide in a test tube and add 1 mL of 10% MgSO Solution.
Allow to Stand for 30 Minutes.
In another Test Tube, place 5 mL 5% gelatin solution and add 1 mL of 10% MgSO.
If no Precipitate forms, add Solid MgSO until saturated.
Decant the Supernatant Liquid from each of the 2 tubes.
Add an Excess of Water and note which Colloid is reversible. ____________________________
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4. Qualitative Determination of the Sign of the Charge on Colloidal Particles by Capillary Analysis:
Filter Paper is negatively charged against water. The Ascent of Colloids to different heights on
Strips of Filter Paper is due to the Difference in their Electrical Charge.
Negatively charged Colloids rise with their Dispersion medium at a Slower Rate than Water.
Positively charged colloids are neutralized at the Point where the Filter Paper touches the surface of
the Solution and only the Dispersion Medium will ascend by Capillary attraction.
4.1 Secure 2-beakers of the Same Size.
4.2 Place 25mL of 0.1% Acid Fuchsin in One, and the Same Volume of 0.1% Methylene Blue on the
Other.
4.3 Suspend a Strip of Filter Paper (15x5 cm.) from a Horizontal Glass Rod, top of the Beaker.
4.4 Allow 2 cm of the Filter to Soak in the Solution.
4.5 Record RESULTS observed. _________________________________________________________

B. SUSPENSIONS:
Suspensoid Particles in water carry electrical Charges and are precipitated by Ions that Carry
charges of opposite Sign but not ions that carry charges of the same sign.
1. Place 5 mL of 1% Ferric Hydroxide in a test tube.
2. Add Normal Saline Solution a Drop at a time counting the number of drop and shaking after each
addition until a permanent precipitate is formed.
C. DIALYSIS:
1.
2.
3.
4.
5.
6.

Place 5 mL of 1% Starch Solution and 5 mL of NaCl Solution in a Cellophane Bag.


Tie the mouth of the Bag and immerse it on a Beaker containing Clean Water.
Allow to stand for 30 40 minutes, before removing the bag from the beaker.
Take 5 mL of the Clean Water from the Beaker and Place it on a Test Tube.
Add 3 5 Drops of Lugols Iodine Solution mix and observe result.
On another tube, place 5 mL of the Clean Water from the beaker and add 3 drops of AgNO and
observe for turbidity or precipitate formation.
Which of the substances inside the bag diffused out of the semi-permeable membrane?
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D. ADSORPTION:
a.
b.
c.
d.
e.

Place 20 mL of 0.01% Aqueous Solution of Methylene Blue in an Erlenmeyer Flask.


Add 10 Micrograms of Animal Charcoal and Shake Vigorously.
Filter and Note the Color of the Filtrate. ___________________________________________
Allow the Filter Paper and its Contents to dry on the Funnel.
Pour 10 mL of 95% Ethyl alcohol over the Animal Charcoal and Collect the filtrate in a Clean test
tube.
How much of the Dye is recovered? ___________________________________________________
Explain the behavior of Methylene Blue towards the Animal Charcoal. ________________________
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f. Place 25 mL of 1% Brown Sugar Solution in a Beaker.
g. Add 1 grams of Powdered Animal Charcoal and Boil for 5 minutes with Occasional Stirring.
h. Filter while hot and note the color of the Filtrate. _______________________________________

E. VISCOSITY:
1. Fill a Clean 10 mL Pipette with Distilled water.
2. Hold it Vertically at Eye Level and allow the contents to drain, timing up with the second hand of
watch, the time required to empty it.
3. Repeat using Ferric Hydroxide Solution.
4. Carefully note the time required to empty the pipette. Compare the result. Obtained for both
solutions.
5. Formulate a Theory to Explain the differences in Viscosities. ____________________________
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6.

IV.

What Conditions affect Viscosity? _________________________________________________


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CONCLUSION:

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