Study the following statement:

Kaji pernyataan berikut:

You are given a sterile nutrient broth and Bacillus subtilis culture (bacteria culture).
''Anda diberikan bubur nutrient steril dan kultur Bacillus subtilis (kultur bakteria).
(a) Suggest one hypothesisto investigate the above statement. [ 1 mark]
Cadangkan satu hipotesis untuk menyiasat pernyataan di atas [1 markah]
Notes: You can use...
1.
the "The higher..............(MV) ..................., the lower............ (RV) ..................//viseversa" statement
OR
2.
You can just COPY the statement in the box
as your answer. Both are accepted!
Answer:
"The higher the light intensity, the lower the growth of Bacillus subtilis"
Remember; use the named organism given in the question.
(b) Describe one experiment to test your hypothesis in l0(a) based on the following criteria:
Huraikan satu eksperimen untuk mengkaji hipotesis di 10(a) berdasarkan kriteria berikut:
(i) Aim of the experiment
Tujuan eksperimen

[1 mark]
[1 markah]

(ii) Identification of variables

Mengenalpasti pembolehubah

[2 marks]
[2 markah]

(iii) List of apparatus and materials

Senarai radas dan bahan

[1 mark]
[1 mark]

(iv) Procedure or method

Prosedur atau kaedah

[4 marks]
[4 markah]

(v) Tabulation of data

Penjadualan data

[1 mark]
[1 mark]

Answer:
(i) Aim: "To study the effect of light intensity on the growth of Bacillus subtilis (bacteria)"

Note: The answer must begin with "To study....." then followed by MV and "on the" RV.
In short, the answer should look like this "To study........MV...... on the..........RV.........."
----------------------------------------------------(ii) Variables:- (the CORRECT way to write the answer)
Manipulated - Light intensity
Responding - The growth of Bacillus subtilis
Constant - (Temperature, humidity, nutrient, pH of nutrient broth) ie. other factors that can affect the
growth of microorganisms, or volume of nutrient broth/bacterial culture or number of days
Notes: PLEASE write down every variable separately! Don't just list all variables in one blow.
For example... (WRONG way to answer)..
Variables: Light intensity, growth of microorganism and volume of nutrient broth.
----------------------------------------------------------------------------(iii) Apparatus and materials: (this time, PLEASE write all apparatus & materials together - to avoid
mistakes)
Bacterial culture, sterile nutrient broth, test tubes, sterile cotton wool, syringe
Note: list all materials & apparatus that are used in the experiment.
-------------------------------------------------------(iv) Procedures: (you can answer in point form)

Set up apparatus as shown in the diagram above (this type of answer must accompanied with a
labelled diagram as above)

Transfer 1 ml (CV) of bacterial culture in each test tube and plug them with sterile cotton wool

Place the test tube in the following places:

A - under bright light

B - on the cabinet

C - in a dark cupboard }

Keep for 2 days (CV)

}
}

MV

Observe the cloudiness of the nutrient broth (RV) in each test tube and record in the table
------------------------------------------------------------------

(v) Data: (in table form - should clearly stated the MV and RV on the title)
(MV)

(RV)