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COURSE CONTENT
THEORY
Biological cell
(a) Architecture, compartmentation, cell membrane structure and functions;
structure- function relationships.
(b) Membrane transport.
Biomolecules
(a) Function and classification of carbohydrates, lipids, protein and amino
acids.
(b) Stereoisomerism and chemistry of monosaccharides, amino acids, and
fatty acids.
(c) Structural organization and structure-function relationships of proteins.
Hemoglobin and myoglobin,
molecular mechanism of O2 transport and storage. Molecular basis of sickle
cell anaemia and
thallesemias.
(d) Molecular mechanism of muscle contraction.
(e) Plasma proteins, their functions and clinical significance.
Enzymes
(a) Nomenclature, classification,
(b) Kinetics, mechanism of enzymatic catalysis.
(c) Factors influencing enzymatic catalyses, enzyme activators and
inhibitors.
(d) Regulation of enzyme activity,
(e) Clinical enzymology, isoenzymes.
Metabolic pathways, their regulation and metabolic
interrelationships
Metabolism: general concepts and characteristics of metabolic pathways.
Carbohydrate metabolism
(a) Pathways of glucose metabolism: glycolysis
(b) HMP shunt
(c) Gluconeogenesis
(d) Glycogenolysis, glycogenesis
(e) Galactose and fructose metabolism
(f) Glycogen storage disease
(g) Inborn errors of glucose metabolism
(h) Regulation of glucose metabolism.
Amino acid metabolism
PRACTICALS
1. Laboratory Instrumentation.
2. Protein fractionation, denaturation, separation of proteins and amino
acids.
3. Colour reactions of amino acids and proteins.
Distribution of Topics
Paper I Cell structure, compartmentation, functions, biological membranes.
Chemistry, functions and metabolism of amino acids, carbohydrates and
lipids, metabolic interrelationships, in born errors of metabolism, nutrients
and malnutrition, biological oxidation, hormones, vitamins and coenzymes.
Paper II Protein structure and functions, hemoglobin, myoglobin, plasma
proteins and iron metabolism, enzymes and enzyme kinetics, nucleic acid
biochemistry and molecular biology, immunology, digestion and absorption,
function Tests: G.I.T., kidney, and liver, regulation of acid base balance,
environmental biochemistry, cancer biochemistry
SEMESTERS
Biochemistry is taught in two semesters in the 1st year of the MBBS
curriculum.
1st Semester : Sept Feb
2nd Semester : Feb Sept
TEACHING-LEARNING METHODOLOGY
1. Didatic lectures: 4 hours /week of interactive classroom lectures to
facilitate learning of terminology, principles and concepts. Books and
resource material are suggested to encourage self- directed learning.
2. Tutorials; Two hours/week. Problem based small group discussions,
questions-answer sessions, revision and reinforcement of difficult concepts
in tutorial hours. The purpose is to inculcate skills of reasoning, meaningful
approaches to learning and facilitate understanding of the subject.
3. Laboratory exercises: (Biochemistry practicals) three hours/week: a) to
substantiate and clarify theoretical concepts with experimental evidence b)
to develop skills of performing basic biochemical tests important in clinical
TEXT-BOOKS RECOMMENDED
Biochemistry Ed. Lubert Stryer. W.H. Freeman and Company, New York.
Principles of Biochemistry. Ed. Lehinger, Nelson and Cox. CBS
Publishers and distributors.
Harpers Biochemistry, Ed. R.K. Murray, D.K. Granner, P.A. Mayes and
V.W. Rodwell. Appleton and Lange, Stamford, Connecticut.
Lippincott's Illustrated Reviews Biochemistry by Denise R. Ferrier ,
Wolter Kluwer; 6 edition
Textbook of Biochemistry with Clinical Correlations. Ed. Thomas M.
Devlin, Wiley-Liss Publishers.
Textbook of Biochemistry for Medical Students by Md Rafi Orient
Blackswan Private Limited - New Delhi
A case oriented approach to Clinical Biochemistry by Namrata
Chhabra , ,Jaypee Brothers Medical Publishers Private Limited
Textbook of Biochemistry for Medical Students by Vasudevan J aypee
Brothers Medical Publishers Private Limited
Biochemistry by U Satyanarayana Elsevier
Essentials of Biochemistry by Pankaja Naik 4 th ed , Jaypee Brothers
Medical Publishers Private Limited
Rapid Review Biochemistry: With Student Consult Online Access by
Pelly Elsevier Health - US
Tietz Textbook of Clinical Chemistry. Ed. Burtis and Ashwood. W.B.
Saunders Company.
Biochemistry. Ed. Donald Voet and Judith G. Voet. John Wiley & Sons,
Inc.
Immunology Ed. 5th Richard A. Goldsby, Thomas J Kindt, Barbara A
Osborne, Janis Kuby.
Immunology Ed. 6th Ivan Roitt, Jonathan Brostoff, David Male.