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ISSN 1303-2712
DOI: 10.4194/1303-2712-v12_2_18
The Effects of Dietary Turnip (Brassica rapa) and Biofuel Algae on Growth
and Chemical Composition in Rainbow Trout (Oncorhynchus mykiss)
Juveniles
Halil brahim Halilolu1,*, Murat Arslan1,2, Bong-Joo Lee3, Konrad Dabrowski3
1
Abstract
The present study was conducted to investigate the supplemental effects of the turnip (Brassica rapa) and its extracts
and algae powder on growth performance and body composition of rainbow trout juveniles (0.670.01 g). For this purpose, 7
casein-gelatin based semi purified diets were formulated to be isonitrogenous and isocaloric to contain turnip meal, its extracts
(with water and methanol) and the powder of after extraction as well as algae powder. The diets were supplemented with
15% wheat flour (diet 1, control), 15% turnip meal of Turkish origin (diet 2), water extract from U.S. turnip (diet 3), methanol
extract from Turkish turnip (diet 4), powder of Turkish turnip after extraction (diet 5), 15% turnip meal of U.S. origin (diet 6)
and 15% biofuel algae powder (diet 7). After 4 week feeding trial, the fish fed diet 7 had significantly lower growth than those
fed the other 6 experimental diets (P<0.05). Among the fish fed dietary turnip or its extracts, there was no significant
difference in growth and survival (P>0.05). However, turnip supplementation tended to increase growth in comparison to
control diet. Food conversion ratio (FCR) and protein efficiency ratio (PER) were significantly higher in fish fed diets with
turnip and its extract than those fed the biofuel algae and control diets. Our study indicated that turnip seems to have a growth
enhancing effects in juvenile rainbow trout. This positive effect might be connected with some phytochemicals, which is
reported as antioxidant and health promoting properties of turnip. Further studies are needed to disclose the effect of dietary
turnip on rainbow trout.
Keywords: Turnip (Brassica rapa), feed, growth, rainbow trout.
Diyetlere lave Edilen algam (Brassica rapa) ve Yosun Ununun Gkkua Alabal (Oncorhynchus
mykiss) Yavrularnn Byme ve Kimyasal Kompozisyonlarna Etkileri
zet
Bu alma yavru gkkua alabalklar (0,670,01) diyetlerine katlan algam (Brassica rapa) ve eksraktlar ile yosun
ununun olumlu etkilerini belirlemek amacyla yaplmtr. Bunun iin 7 adet kazein-jelatin tabanl enerji ve besin deerleri
ayarlanm algam unu, ektraktlar (su ve metanol), ektraksiyon sonras toz hali ve yosun unu ile yar saf diyetler
hazrlanmtr. Diyetler %15 buday unu (diyet 1, control), %15 Trkiye orijinli algam unu (diyet 2), Amerika orijinli su
eksrakt (diyet 3), Trkiye orijinli metanol ekstrakt (diyet 4), Trkiye orijinli eksraktsiyon sonras tozu (diyet 5), %15
Amerika orijinli algam unu (diyet 6) ve %15 lik alg unu (diyet 7) ilavesiyle hazrlanmtr. Drt haftalk besleme sresi
sonunda 7 numaral diyetle beslenen balklarda byme dier 6 diyetle beslenenlere gre nemli seviyede dk kmtr
(P<0,05). algam ve onun eksraktlarnn kullanld diyetlerdeki byme ve yaama oranlar bakmndan nemli bir fark
kmamtr (P>0,05). Yem deerlendirme (FCR) ve protein etkinlik deerleri (PER) algam ve ektsraktlar katlan gruplarda
yosun unu ve kontrol grubuna gre nemli seviyede yksek kmtr. almamz, algam unu takviyesinin alabalk
yavrularnda bymeyi tevik etme eiliminde olduunu gstermektedir. algam ununun alabalklar zerindeki pozitif
etkilerinin daha iyi anlalmas iin detayl almalarn yaplmas gerekmektedir.
Anahtar Kelimeler: algam (Brassica rapa), yem, byme, gkkua alabal.
Introduction
Replacement of fish meal by ingredients of plant
or animal origin in aquatic animal feeds has been the
constant objective of numerous recent nutritional
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H.. Haliloglu et al. / Turk. J. Fish. Aquat. Sci. 12: 323-329 (2012)
H.. Haliloglu et al. / Turk. J. Fish. Aquat. Sci. 12: 323-329 (2012)
325
Table 1. Composition (%) of the seven experimental diets fed to rainbow trout
Ingredients
Casein (vitamin free)a
Gelatina
L-Arga
L-Meta
L-Lysa
CPSPb
Dextrin
Wheat flour
Turnip meal (Turkey)
Turnip meal extract (H2O)
Turnip meal extract (MeOH)
Turnip meal post extract
Turnip meal (USA)
Biofuel algaec
Cod liver oil
Soy-lecithin
Vitamin mixtured
Mineral mixturee
CMCf
Stay-C 35g
Choline cloride
1
40.00
8.00
0.50
0.40
0.80
5.00
9.10
15.00
2
40.00
8.00
0.50
0.40
0.80
5.00
9.10
3
40.00
8.00
0.50
0.40
0.80
5.00
9.10
7.00
Diets
4
40.00
8.00
0.50
0.40
0.80
5.00
9.10
12.30
5
40.00
8.00
0.50
0.40
0.80
5.00
9.10
10.70
6
40.00
8.00
0.50
0.40
0.80
5.00
9.10
0.00
7
40.00
8.00
0.50
0.40
0.80
5.00
9.10
0.00
15.00
8.00
2.70
4.00
15.00
10.00
4.00
2.00
3.00
2.00
0.06
0.17
10.00
4.00
2.00
3.00
2.00
0.06
0.17
10.00
4.00
2.00
3.00
2.00
0.06
0.17
10.00
4.00
2.00
3.00
2.00
0.06
0.17
10.00
4.00
2.00
3.00
2.00
0.06
0.17
10.00
4.00
2.00
3.00
2.00
0.06
0.17
15.00
10.00
4.00
2.00
3.00
2.00
0.06
0.17
Turkish turnip
9.97
8.76
2906
26285
4310
1308
57.1
21.6
79.6
10.3
564
33.57
U.S. turnip
11.67
11.31
5358
32239
3719
1366
38.4
10.96
63.7
13.5
1866
24.94
Biofuel algea
15.3
6.95
4313
2189
40776
1679
1659
16
2159
309
701
299
326
H.. Haliloglu et al. / Turk. J. Fish. Aquat. Sci. 12: 323-329 (2012)
1
58.71
15.09
5.03
8102
3082
5097
277
42
91
88
2371
41
2
59.52
14.95
5.06
7808
6211
5004
402
292
112
83
2222
108
3
55.74
15.08
5.92
8925
7937
5347
433
44
82
87
2550
45
Diets
4
56.73
15.12
5.53
8738
2997
5501
279
17
144
92
2409
38
5
56.79
15.03
5.07
8992
3958
5926
389
38
88
93
2357
48
6
54.87
14.89
5.93
9514
8211
5820
436
31
116
91
2553
43
7
57.35
15.11
13.21
9692
3147
10895
632
51
5510
157
2478
115
Discussion
Statistical Analysis
Results were expressed as MeanSD (n=3). Data
were subjected to one-way ANOVA and subsequent
comparison of Means by Duncans multiple range
test. Percentage data were arcsin transformed prior to
statistical analysis. Differences were considered
statistically significant at P<0.05.
Results
At the end of the feeding trial, dietary
supplementation had significant effect on growth
performance (Table 4) and body chemical
composition (Table 5) in rainbow trout juveniles
(P<0.05). Fish fed diet 7 exhibited significantly lower
H.. Haliloglu et al. / Turk. J. Fish. Aquat. Sci. 12: 323-329 (2012)
327
Table 4. Growth performance and survival of rainbow trout fed the four experimental diets. Means with different superscript
letters in a row are significantly different (P<0.05)
Diets
4
5
2.260.12a
2.190.26a
337.3719.34a 327.0140.08a
4.910.14a
4.830.31a
91.667.63
8515
0.610.01bc
0.620.01b
3.240.07ab
3.180.07b
1
2
3
Final weight (g)
2.040.20ab
2.100.12a
2.290.22a
Weight gain (%)
305.0931.26a 325.1217.66a 341.6233.59a
SGR1 (%/day)
4.650.25a
4.820.14a
4.940.24a
Survival (%)
91.665.77
955
908.66
FCR2
0.630.01b
0.610.05bc
0.620.01bc
PER3
3.160.05b
3.250.05ab
3.20.07b
1
Spesific growth rate, 2Food conversion ratio, 3Protein efficiency ratio
6
2.320.05a
346.828.44a
4.990.06a
91.662.88
0.590.05c
3.380.05a
7
1.730.14b
258.5421.4b
4.250.19b
96.665.77
0.670.04a
2.980.18c
Table 5. Proximate and mineral composition (% of dry matter) of whole body of rainbow trout. Means with different
superscript letters in a row are significantly different (P< 0.05)
Diets
Crude
protein (%)
Lipid (%)
NL (%)
PL (%)
Ash (%)
P (g/g)
K (g/g)
Ca (g/g)
Mg (g/g)
S (g/g)
Bo (g/g)
Cu (g/g)
Fe (g/g)
Mn (g/g)
Na (g/g)
Zn (g/g)
Initial
68.694.2a
58.623.8cde
60.644.1b
59.833.9b
57.782.6de
56.982.4e
59.393.1bc
59.083.2bcd
3.160.1d
62.801.4d
37.191.4a
13.660.56 a
1916935a
1752042a
2231075a
144724a
878814a
56.181.2a
3.640.9b
86.811.3b
6.562.1f
656425a
294.241.2a
7.300.38a
84.390.57a
15.600.57d
9.470.56de
1362547cd
1162755bc
14525934d
85352cd
6514165bc
12.315.2b
8.962.8b
55.2613.8b
21.042.7b
4130329b
150.92.6a
6.400.23c
82.051.48c
17.941.48b
9.930.01bcd
14316 310c
12865750b
15415487cd
98136bc
6526277bc
15.837.8b
18.250.1a
43.365.5b
14.71.7cd
4048215b
162.1712.4a
6.50.24bc
82.530.74c
17.460.74b
10.530.05b
15567695b
12349925b
17358148bc
93989bc
6498230bc
8.927.4b
6.912.1b
45.717.9b
16.370.8c
3797304b
167.454.1a
7.460.21a
83.900.54ab
16.090.54cd
9.120.28e
13022338d
10757453c
12979130e
79147d
6268106bc
6.962.5b
7.590.7b
43.542.1b
25.821.5a
3846242b
146.765.2a
7.550.83a
84.100.63ab
15.890.63cd
9.850.25cd
13999586cd
10850861c
15009129d
87787bcd
6229103c
13.143.3b
5.551.7b
41.574.3b
15.590.9c
3675400b
154.554.3a
6.550.33bc
82.940.58bc
17.050.58bc
10.470.45bc
15988 954b
12336951b
18519132b
99681b
6488379bc
12.516.3b
10.311.6ab
51.807.4b
12.470.7de
3847317b
168.2910.8a
7.00.30ab
84.511.47a
15.481.47d
9.880.38cd
13858722cd
11509369bc
14625201d
94727bc
6858571b
10.427.4b
19.254.5a
282.7673.8a
11.740.8e
4185828b
159.889a
328
H.. Haliloglu et al. / Turk. J. Fish. Aquat. Sci. 12: 323-329 (2012)
rainbow trout.
Acknowledgements
The senior author was supported by The
Scientific and Technological Research Council of
Turkey (TUBITAK).
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