Emily Wignall

Microbiology
Identification of Unknowns
23
Negative (Pink)
Rod Shape
Non-spore forming

61
Negative (Pink)
Rod Shape
Non-spore forming

(2-6) Oxygen Requirements

Facultative
-growth throughout length of
stab in agar

Facultative
-growth throughout length of
stab in agar

(5-2) Oxidation Fermentation

Tests

With oil (Yellow)=Positive

Without oil (Yellow)= Positive
Capable of oxidative
and fermentative
utilization of glucose

With oil (Yellow)=Positive

Without oil (Yellow)= Positive
Capable of oxidative
and fermentative
utilization of glucose

(5-3) Phenol Red Broth

Sucrose= Positive
Yellow & bubble in tube
A/G
Fermentation with acid
& gas end products
Mannitol= Negative
No color change or
bubbles
-/ No fermentation
Lactose= Negative
No color change or
bubble
No fermentation
Dextrose/Glucose= Positive
Yellow & bubble in tube
A/G
Fermentation with acid
& gas end products

Sucrose= Positive
Yellow & bubble in tube
A/G
Fermentation with acid
& gas end products
Mannitol= Negative
No color change or
bubbles
-/ No fermentation
Lactose= Negative
No color change or
bubble
No fermentation
Dextrose/Glucose= Positive
Yellow & bubble in tube
A/G
Fermentation with acid
& gas end products

(5-4) MRVP

MR= Negative
(Yellow) No color
change
No mixed acid
fermentation
VP= Positive
(Red) Color change
2,3-butanediol
fermentation

MR= Negative
(Yellow) No color
change
No mixed acid
fermentation
VP= Positive
(Red) Color change
2,3-butanediol
fermentation

Gram Stain
Negative Stain
Spore

Emily Wignall
Microbiology
Identification of Unknowns

(5-5) ONPG

(5-6) Catalase Test

(5-7) Oxidase Test

(5-8) Nitrate Reduction

(5-9) Citrate Test

(5-11) Decarboxylation Test

(5-12) Phenylanine Deaminase

Test
(5-13) Starch Hydrolysis
(5-14) DNA Hydrolysis

(acetoin produced)
Positive
Yellow color formation
Produces Betagalactosidase
Positive
Bubbles
Catalase is present
Only present in Obligate
aerobe, facultative
anaerobe, and small
amounts in
microaerophile
Negative
Does not produce
cytochrome c oxidase
Positive
Nitrate reduction to
nitrite (NO 3- to NO2-)
No gas production

(acetoin produced)
Positive
Yellow color formation
Produces Betagalactosidase
Positive
Bubbles
Catalase is present
Only present in Obligate
aerobe, facultative
anaerobe, and small
amounts in
microaerophile
Negative
Does not produce
cytochrome c oxidase
Positive
Nitrate reduction to
nitrite (NO 3- to NO2-)
No gas production

Positive
Blue
Citrate is utilized
Ligase= Positive
Purple
Organism produces the
specific decarboxylase
enzyme
Ornithine=Positive
Purple
Organism produces the
specific decarboxylase
enzyme
23 is more a more grey
cloudy than 61
Arginine= Negative
Brownish color same as
negative control
No decarboxylation
Negative
Yellow (No color
change)
Negative
Negative

Positive
Blue
Citrate is utilized
Ligase= Positive
Purple
Organism produces the
specific decarboxylase
enzyme
Ornithine=Positive
Purple
Organism produces the
specific decarboxylase
enzyme
61 is a clearer/brighter
purple than 23
Arginine= Negative
Yellow
Fermentation, no
decarboxylation
Negative
Yellow (No color
change)
Negative
Negative

Emily Wignall
Microbiology
Identification of Unknowns

(5-15) Lipid Hydrolysis

(5-17) Gelatin Hydrolysis

(5-18) Urea Hydrolysis

(5-21) TSIA

No halo
Negative
Lipase is absent
Negative
Did not hydrolyze
gelatin
Negative
Remained clear/light
pink
Red Slant Yellow Butt
K/A
Glucose fermentation
with acid production;
proteins catabolized
aerobically (in the slant)
with alkaline products
Cracks & Lifting in Agar
Gas Production

No halo
Negative
Lipase is absent
Negative
Did not hydrolyze
gelatin
Negative
Remained clear/light
pink
Yellow Slant Yellow Butt
A/A
Glucose and lactose
and/ or sucrose
fermentation with acid
accumulation in slant
and butt
Cracks & Lifting in Agar
Gas Production