Professional Documents
Culture Documents
Fischer et al.
(54)
(75)
Inventors:
Publication Classi?cation
(51)
Int. Cl.
C12Q 1/70
C12Q 1/68
(52)
(2006.01)
(2006.01)
TX (U S)
(57)
(73) Assignee:
13/332,204
(22)
Filed:
ABSTRACT
(63)
(60)
1, 2007.
US 2012/0100529 A1
Delta Rn vs Cycle
10006-000
OneStep+\
_
10006-001
Z?
3; 10006-002
2
/ ////
//7/,/
//>Cj%
/
/,<5
10006-003
10006-004
2K7
/
13 5 7 9111315171921232527293133353739
FIG.1
12110111115611; /
US 2012/0100529 A1
Am bion
Al/NCD
j MugMux
ep-
One- ep+
Real-Time CT
Vqlue
19.25
19.08
5285*
M pies
47 x109
55 x109
2.5 x105
Detected
5059*
25.95
21.25
/////////////////////// ///
///
///
US 2012/0100529 A1
///
///
One-Step Solution
23.97
21.37
31.38
25.35
32.70
32.20
24.39
32.17
32.27
(Ambion)
Water
FIG.3
US 2012/0100529 A1
US 2012/0100529 A1
95(3)];
FIG.5
US 2012/0100529 A1
1O \\\\\\\\\\\\\\\\\\\x\\\w&\\\wm\\\wm\w
\
MEDIA
\\
L|_
C.)
a:
t,
PnmeScoreTM
|
" ///////J7/////////////
10
/////////////
20
3O
40
50
Seconds in Prime Store
FIG.6
60
E .B+wQE
2P2059-21
US 2012/0100529 A1
Day 0
Day 30
FIG.7B
US 2012/0100529 A1
086m+wEoi_ywBEtun_:+
cmatom2&0m>
owed
S296E0521
08.0
Q23
US 2012/0100529 A1
296625.2
4% Agarose (HR)
1
3 4 5 6
FIG.8C
US 2012/0100529 A1
US 2012/0100529 A1
5@oE.ma8T3ol1ws8c
QUE
mm
on
mm
1
km
'
FIG.'IO
US 2012/0100529 A1
US 2012/0100529 A1
+ TCEP
TChrietscoald
5mM
10mM
25MM
35mM
Molar Concentration
FIG.11
50mM
US 2012/0100529 A1
G2O8ES.
2150
T% P
5mM
10mM
25MM
35mM
Molar Concentration
FIG.12
50mM
33
35
FIG.13A
40
35
3O
25
2O
15
1O
5
O
Whole Virus
,1
33
FIG.13B
35
US 2012/0100529 A1
US 2012/0100529 A1
Blood Tube
'
'
if?)77/27727797/72152
///,///////////,//f///7
PrimeStoreTM
34.7485
AVG:
2223426445,
//// /,////////////,//'/
Qiogen
/
AVG:
///
7/9
//
NoHeporingi';
///
31.9677
32.5538
33.118
32.0509
32.8927
~\\\\\\\\\\\\\\\\\\\\\
32.984
32.009
\ \\\\\\\\\
\\\\\\\\\\
35.3054
33.4051
34.4903
34.6428
34.7485
33.4051
35.0378
35.3937
my
'
//
///
FIG.14 B
\ \
35.01825???
//////////' ,
/ / /
/ / /
/ /
/ /
US 2012/0100529 A1
/////////
ZZZZZZZZZgPrimeStoreTLBlood Extroction:;;;;;;;
////////
1pg
32.7715
33.2488
\ \\\\\_33.01015
0.337502067
0.1pg
36.434
37.0442
36.7391
0.431 476558
AVG
STDEV
1pg
0.1pg
35.8461
37.01
37.5771
38.1878
36.42805\
\\37.88245
AVG
0.823001583
0.4318301 1 1
STDEV