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This article contains a list of the most studied restriction enzymes whose names start with A. It contains approximately 30 enzymes.
The following information is given:
Enzyme: Accepted name of the molecule, according to the internationally adopted nomenclature[1][2], and bibliographical references.
(Further reading: see the section "Nomenclature" in the article "Restriction enzyme".)
PDB code: Code used to identify the structure of a protein in the PDB database of protein structures. The 3D atomic structure of a
protein provides highly valuable information to understand the intimate details of its mechanism of action[3][4].
Source: Organism that naturally produces the enzyme.
Recognition sequence: Sequence of DNA recognized by the enzyme and to which it specifically binds.
Cut: Cutting site and DNA products of the cut. The recognition sequence and the cutting site usually match, but sometimes the cutting
site can be dozens of nucleotides away from the recognition site[5][6].
Isoschizomers and neoschizomers: An isoschizomer is an enzyme that recognizes the same sequence as another. A neoschizomer is a
special type of isoschizomer that recognizes the same sequence as another, but cuts in a different manner. A maximum number of 8-10
most common isoschizomers are indicated for every enzyme but there may be many more. Neoschizomers are shown in bold and green
color font (e.g.: BamHI). When "None on date" is indicated, that means that there were no registered isoschizomers in the databases on
that date with a clearly defined cutting site. Isoschizomers indicated in white font and grey background correspond to enzymes not
listed in the current lists:
as in this not listed enzyme: EcoR70I
Legend of nucleobases
Nucleotide
represented
Code
A
Adenine (A)
Cytosine (C)
Thymine (T)
Guanine (G)
A, C, G or T
A or C
A or G
A or T
C or T
C or G
A, C or T
A, C or G
G or T
Contents
C, G or T
A, G or T
Restriction enzymes
Enzyme
Source
Recognition sequence
Cut
AaaI
Arthrobacter aurescens
5' TGTACA
5' ---T
AatII [7]
5' GACGTC
5' ---GACGT
3' ---C
TGCAG--- 5'
AbaI
5' TGATCA
5' ---T
GATCA--- 3'
AbeI
5' CCTCAGC
5' ---CC
AbrI
Azospirillum brasilense
5' CTCGAG
5' ---C
TCGAG--- 3'
[10][11]
Acinetobacter calcoaceticus
5' GTMKAC
5' ---GT
MKAC--- 3'
FblI, XmiI
AccII [8][12]
Acinetobacter calcoaceticus
5' CGCG
5' ---CG
CG--- 3'
AccIII [13]
Acinetobacter calcoaceticus
5' TCCGGA
5' ---T
Acc16I
Acinetobacter calcoaceticus 16
5' TGCGCA
5' ---TGC
Acc36I [14]
Acinetobacter calcoaceticus 36
5' ACCTGC
5' ---ACCTGCN3N
AccI [8][9]
PDB code
3' ACATGT
3' CTGCAG
3' ACTAGT
3' GGAGTCG
3' GAGCTC
3' CAKMTG
3' GCGC
3' AGGCCT
3' ACGCGT
3' TGGACG
3' ---ACATG
3' ---ACTAG
3' ---GGAGT
3' ---GAGCT
3' ---CAKM
3' ---GC
3' ---AGGCC
Isoschizomers
GTACA--- 3'
T--- 5'
C--- 3'
T--- 5'
TCAGC--- 3'
CG--- 5'
C--- 5'
TG--- 5'
GC--- 5'
T--- 5'
GCA--- 3'
CGT--- 5'
3' ---TGGACGN3NNNNN
CCGGA--- 3'
3' ---ACG
BbvCI
NNNN--- 3'
--- 5'
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Acc65I [15]
Acinetobacter calcoaceticus 65
5' GGTACC
5' ---G
Acc113I
5' AGTACT
5' ---AGT
AccB1I
Acinetobacter calcoaceticus B1
5' GGYRCC
5' ---G
AccB2I
Acinetobacter calcoaceticus B2
5' RGCGCY
5' ---RGCGC
AccB7I [16]
Acinetobacter calcoaceticus B7
5' CCAN5TGG
5' ---CCANNNN
AccBSI [17]
Acinetobacter calcoaceticus BS
5' CCGCTC
5' ---CCG
AccEBI
5' GGATCC
5' ---G
GATCC--- 3'
AceI
Anabaena cedrorum
5' GCWGC
5' ---G
CWGC--- 3'
AceII
Anabaena cedrorum
5' GCTAGC
5' ---GCTAG
AceIII
Anabaena cedrorum
5' CAGCTC
5' ---CAGCTCN4NN
AciI [18][19]
Arthrobacter citreus
5' CCGC
5' ---C
AclI [20]
Acinetobacter calcoaceticus M4
5' AACGTT
5' ---AA
CGTT--- 3'
AclNI
5' ACTAGT
5' ---A
CTAGT--- 3'
AclWI
5' GGATC
5' ---GGATCNNNN
AcpI
5' TTCGAA
5' ---TT
AcpII
5' CCAN5TGG
5' ---CCANNNN
AcrII
Anabaenopsis circularis
5' GGTNACC
5' ---G
GTNACC--- 3'
AcsI [21]
5' RAATTY
5' ---R
AATTY--- 3'
AcuI
Acinetobacter calcoaceticus
5' CTGAAG
5' ---CTGAAGN12NNNN
AcvI
Aeromonas caviae
5' CACGTG
5' ---CAC
AcyI [22]
Anabaena cylindrica
5' GRCGYC
5' ---GR
AdeI
5' CACNNNGTG
5' ---CACNNN
AeuI
Achromobacter eurydice
5' CCWGG
5' ---CC
WGG--- 3'
AfaI [23]
5' GTAC
5' ---GT
AC--- 3'
Afa22MI
Acidocella facilis
5' CGATCG
5' ---CGAT
Afa16RI
5' CGATCG
5' ---CGAT
3' CCATGG
3' TCATGA
3' CCRYGG
3' YCGCGR
3' GGTN5ACC
3' GGCGAG
3' CCTAGG
3' CGWCG
3' CGATCG
3' GTCGAG
3' GGCG
3' TTGCAA
3' TGATCA
3' CCTAG
3' AAGCTT
3' GGTN5ACC
3' CCANTGG
3' YTTAAR
3' GACTTC
3' GTGCAC
3' CYGCRG
3' GTGNNNCAC
3' GGWCC
3' CATG
3' GCTAGC
3' GCTAGC
3' ---CCATG
GTACC--- 3'
3' ---Y
TGA--- 5'
G--- 5'
3' ---C
NTGG--- 3'
NNNNACC--- 5'
CTC--- 3'
3' ---TTGC
3' ---TGATC
G--- 5'
G--- 5'
3' ---AAGC
3' ---GGTN
3' ---CCANTG
3' ---YTTAA
GATCG--- 5'
NNNN--- 3'
--- 5'
CGC--- 3'
3' ---CYGC
3' ---GTG
3' ---GGW
3' ---CA
3' ---GC
3' ---GC
BspOI, LlaG2I,
G--- 5'
Psp1406I
AA--- 5'
A--- 5'
N--- 3'
--- 5'
TT--- 5'
NTGG--- 3'
NNNNACC--- 5'
G--- 5'
R--- 5'
--- 3'
NN--- 5'
GTG--- 3'
CAC--- 5'
CGYC--- 3'
RG--- 5'
GTG--- 3'
NNNCAC--- 5'
CC--- 5'
TG--- 5'
CG--- 3'
TAGC--- 5'
CG--- 3'
TAGC--- 5'
AlwI,
BinI,
BpuFI,
BsrWI,
BspPI,
CGAA--- 3'
3' ---GACTTCN12NN
3' ---GTG
AsuNHI,
BmtI,
NheI,
PstNHI
C--- 3'
3' ---CCTAGNNNNN
GAG--- 5'
3' ---GTCGAGN4NNNNNN
3' ---GGC
Y--- 3'
CGCGR--- 5'
3' ---GGC
3' ---CGWC
GYRCC--- 3'
3' ---GGTN
3' ---CCTAG
ACT--- 3'
3' ---TCA
3' ---CCRYG
G--- 5'
BspKT5I, Eco57I
BbrPI, BcoAI, Eco72I, PmaCI,
PmlI, PspCI
AhaII, AosII, BbiII, BsaHI,
BstACI, Hin1I, HgiI, Hsp92I,
Msp17I
BstIZ316I, DraIII
AglI, AjnI, BseBI, BstNI, BstOI,
Bst2UI, EcoRII, SleI, SspAI
Csp6I, CviQI, CviRII, HpyBI,
PabI, PlaAII, RsaI, RsaNI
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AfeI [24]
5' AGCGCT
5' ---AGC
AflI [25]
Anabaena flos-aquae
5' GGWCC
5' ---G
GWCC--- 3'
Anabaena flos-aquae
5' CTTAAG
5' ---C
TTAAG--- 3'
[25][28]
Anabaena flos-aquae
5' ACRYGT
5' ---A
CRYGT--- 3'
AgeI [18][29]
Agrobacterium gelatinovorum
5' ACCGGT
5' ---A
CCGGT--- 3'
AglI[30]
Arthrobacter globiformis
5' CCWGG
5' ---CC
WGG--- 3'
AhaI
Alteromonas haloplanktis B8
5' CCSGG
5' ---CC
SGG--- 3'
AhaII
Aphanothece halophytica
5' GRCGYC
5' ---GR
CGYC--- 3'
[10][26]
Aphanothece halophytica
5' TTTAAA
5' ---TTT
AhaB8I
Aphanothece halophytica
5' GGTACC
5' ---G
AhdI [32][33]
Aeromonas hydrophila
5' GACN5GTC
5' ---GACNNN
AhlI
Alteromonas haloplanktis SP
5' ACTAGT
5' ---A
CTAGT--- 3'
AhyI
Aeromonas hydrophila
5' CCCGGG
5' ---C
CCGGG--- 3'
AitI
Aquaspirillum itersonii
5' AGCGCT
5' ---AGC
AjnI
Acinetobacter johnsonii R2
5' CCWGG
5' ---
AjoI
Acinetobacter johnsonii 35
5' CTGCAG
5' ---CTGCA
AleI
Aureobacterium liquefaciens
5' CACN4GTG
5' ---CACNN
AlfI
Acinetobacter lwoffii BH 32
5' GCAN6TGC
5' ---GCAN6TGCN9NNN
AliI
Acetobacter liquefaciens
5' GGATCC
5' ---G
AliAJI
5' CTGCAG
5' ---CTGCA
AloI [34][35]
5' GAACN6TCC
5' ---GAACN6TCCN6NNNNNN
[36][37]
Arthrobacter luteus
5' AGCT
5' ---AG
AlwI
Acinetobacter lwoffii
5' GGATC
5' ---GGATCN3N
5' GWGCWC
5' ---GWGCW
AflII
[25][26][27]
AflIII
AhaIII
[27][31]
AluI [8][10]
[38][39]
Alw21I
3' TCGCGA
3' CCWGG
3' GAATTC
3' TGYRCA
3' TGGCCA
3' GGWCC
3' GGSCC
3' CYGCRG
3' AAATTT
3' CCATGG
3' CTGN5CAG
3' TGATCA
3' GGGCCC
3' TCGCGA
3' GGWCC
3' GACGTC
3' GTGN4CAC
3' CGTN6ACG
3' CCTAGG
3' GACGTC
3' CTTGN6AGG
3' TCGA
3' CCTAG
3' CWCGWG
GCT--- 3'
3' ---TCG
3' ---CCWG
3' ---GAATT
3' ---TGYRC
3' ---TGGCC
AAA--- 3'
TTT--- 5'
GTACC--- 3'
G--- 5'
NNGTC--- 3'
NNNCAG--- 5'
A--- 5'
C--- 5'
GCT--- 3'
CCWGG--- 3'
--- 5'
G--- 3'
NNGTG--- 3'
OliI
ACGTC--- 5'
NNCAC--- 5'
--- 3'
NN--- 5'
GATCC--- 3'
G--- 5'
3' ---CTTGN6AGGN6N
GA--- 5'
3' ---CCTAGN3NN
--- 3'
NNNNN--- 5'
CT--- 3'
N--- 3'
--- 5'
C--- 3'
WCGWG--- 5'
G--- 3'
ACGTC--- 5'
3' ---TC
CGA--- 5'
3' ---CGTN6ACGN9N
3' ---C
RG--- 5'
3' ---GTGNN
3' ---G
AseII,
BpuMI, CauII,
BcnI,
Eco1831I, EcoHI, HgiS22I,
NciI
CC--- 5'
3' ---TCG
3' ---CCTAG
CC--- 5'
3' ---CTGNN
3' ---G
A--- 5'
3' ---AAA
3' ---GGWCC
Asp90I
A--- 5'
3' ---CYGC
3' ---GGGCC
C--- 5'
3' ---GGS
3' ---TGATC
G--- 5'
3' ---GGW
3' ---CCATG
CGA--- 5'
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5' GTCTC
5' ---GTCTCN
Alw44I
5' GTGCAC
5' ---G
AlwNI [41]
Acinetobacter lwoffii N
5' CAGNNNCTG
5' ---CAGNNN
AlwXI
Acinetobacter lwoffii X
5' GCAGC
5' ---GCAGCN7N
Ama87I
Alteromonas macleodii 87
5' CYCGRG
5' ---C
AocI
Anabaena sp.
5' CCTNAGG
5' ---CC
AocII
Anabaena sp.
5' GDGCHC
5' ---GDGCH
3' ---C
HCGDG--- 5'
AorI
5' CCWGG
5' ---CC
WGG--- 3'
Aor13HI [42]
5' TCCGGA
5' ---T
CCGGA--- 3'
Aor51HI [43]
5' AGCGCT
5' ---AGC
GCT--- 3'
AosI
Anabaena oscillarioides
5' TGCGCA
5' ---TGC
GCA--- 3'
AosII
Anabaena oscillarioides
5' GRCGYC
5' ---GR
[18][27][44]
5' GGGCCC
5' ---GGGCC
ApaBI [45]
Acetobacter pasteurianus B
5' GCAN5TGC
5' ---GCANNNNN
ApaCI
Acetobacter pasteurianus C
5' GGATCC
5' ---G
GATCC--- 3'
[46][47]
Acetobacter pasteurianus
5' GTGCAC
5' ---G
TGCAC--- 3'
ApaORI
Acetobacter pasteurianus
5' CCWGG
5' ---CC
WGG--- 3'
ApeKI [48]
Aeropyrum pernix K1
5' GCWGC
5' ---G
CWGC--- 3'
ApiI
Arthrobacter picolinophilus
5' CTGCAG
5' ---CTGCA
3' ---G
ACGTC--- 5'
ApoI [49]
Arthrobacter protophormiae
5' RAATTY
5' ---R
AATTY--- 3'
ApyI
Arthrobacter pyridinolis
5' CCWGG
5' ---CC
WGG--- 3'
AquI
5' CYCGRG
5' ---C
YCGRG--- 3'
AscI [18][50]
Arthrobacter sp.
5' GGCGCGCC
5' ---GG
CGCGCC--- 3'
AseI [51]
Aquaspirillum serpens
5' ATTAAT
5' ---AT
TAAT--- 3'
AseII [51]
Aquaspirillum serpens
5' CCSGG
5' ---CC
SGG--- 3'
AsiI
Azotobacter sp.
5' GGATCC
5' ---G
GATCC--- 3'
ApaI
ApaLI
3' CAGAG
3' CACGTG
3' GTCNNNGAG
3' CGTCG
3' GRGCYC
3' GGANTCC
3' CHCGDG
3' GGWCC
3' AGGCCT
3' TCGCGA
3' ACGCGT
3' CYGCRG
3' CCCGGG
3' CGTN5ACG
3' CCTAGG
3' CACGTG
3' GGWCC
3' CGWCG
3' GACGTC
3' YTTAAR
3' GGWCC
3' GRGCYC
3' CCGCGCGG
3' TAATTA
3' GGSCC
3' CCTAGG
NNNN--- 3'
BcoDI, BscQII,
Alw26I [40]
3' ---CAGAGNNNNN
3' ---CACGT
--- 5'
TGCAC--- 3'
G--- 5'
CTG--- 3'
3' ---GTC
NNNGAC--- 5'
3' ---CGTCGN7NNNNN
3' ---GRGCY
C--- 5'
TNAGG--- 3'
CC--- 5'
C--- 3'
3' ---GGW
CC--- 5'
T--- 5'
3' ---TCG
CGA--- 5'
3' ---ACG
CGT--- 5'
CGYC--- 3'
3' ---CYGC
3' ---C
3' ---CGT
3' ---CCTAG
3' ---CACGT
3' ---GGW
3' ---CGWC
3' ---YTTAA
3' ---GGW
3' ---GRGCY
3' ---CCGCGC
3' ---TAAT
3' ---GGS
3' ---CCTAG
--- 5'
YCGRG--- 3'
3' ---GGANT
3' ---AGGCC
NNNN--- 3'
RG--- 5'
C--- 3'
CCGGG--- 5'
TGC--- 3'
NNNNNACG--- 5'
G--- 5'
G--- 5'
CC--- 5'
G--- 5'
G--- 3'
R--- 5'
CC--- 5'
C--- 5'
GG--- 5'
TA--- 5'
CC--- 5'
G--- 5'
AquI,
BmeT110I, BsiHKCI,
AvaI,
BsoBI, Eco88I, Nli3877I,
NspSAI
BcoI,
BmeT110I,
BsmAI, BsoMAI, BsoBI, BstSI,
Eco27kI, OfoI
PalAI, SgsI
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5' ACCGGT
5' ---A
AsiSI
Arthrobacter sp.
5' GCGATCGC
5' ---GCGAT
AsnI [52]
5' ATTAAT
5' ---AT
AspI [53]
5' GACNNNGTC
5' ---GACN
Asp700I [54]
5' GAAN4TTC
5' ---GAANN
NNTTC--- 3'
Asp713I
5' CTGCAG
5' ---CTGCA
3' ---G
G--- 3'
ACGTC--- 5'
Asp718I [55]
5' GGTACC
5' ---G
GTACC--- 3'
Asp745I
5' GGWCC
5' ---G
GWCC--- 3'
AspAI
Alcaligenes sp.
5' GGTNACC
5' ---G
GTNACC--- 3'
AspA2I
Arthrobacter sp. A2
5' CCTAGG
5' ---C
CTAGG--- 3'
AspEI [56]
Aureobacterium sp.
5' GACN5GTC
5' ---GACNNN
3' ---CTGNN
NNNCAG--- 5'
Achromobacter sp. H
5' GWGCWC
5' ---GWGCW
3' ---C
C--- 3'
WCGWG--- 5'
Asp10HI
5' TTCGAA
5' ---TT
CGAA--- 3'
Asp10HII
5' CCAN5TGG
5' ---CCANNNN
Asp26HI
5' GAATGC
5' ---GAATGCN
Asp27HI
5' GAATGC
5' ---GAATGCN
Asp35HI
5' GAATGC
5' ---GAATGCN
Asp36HI
5' GAATGC
5' ---GAATGCN
Asp40HI
5' GAATGC
5' ---GAATGCN
Asp50HI
5' GAATGC
5' ---GAATGCN
AspLEI
5' GCGC
5' ---GCG
AspMI
Acinetobacter sp. M
5' AGGCCT
5' ---AGG
AspMDI
5' GATC
5' ---
AspNI
Anabaena sp. J3
5' GGNNCC
5' ---GGN
AspS9I
Arthrobacter sp. S9
5' GGNCC
5' ---G
AssI
Arthrobacter sp.
5' AGTACT
5' ---AGT
AstWI
Anabaena sp.
5' GRCGYC
5' ---GR
AspHI
[57]
3' TGGCCA
3' CGCTAGCG
3' TAATTA
3' CTGNNNCAG
3' CTTN4AAG
3' GACGTC
3' CCATGG
3' CCWGG
3' CCANTGG
3' GGATCC
3' CTGN5CAG
3' CWCGWG
3' AAGCTT
3' GGTN5ACC
3' CTTACG
3' CTTACG
3' CTTACG
3' CTTACG
3' CTTACG
3' CTTACG
3' CGCG
3' TCCGGA
3' CTAG
3' CCNNGG
3' CCNGG
3' TCATGA
3' CYGCRG
CCGGT--- 3'
AsiAI
3' ---TGGCC
A--- 5'
CGC--- 3'
3' ---CGC
TAGCG--- 5'
TAAT--- 3'
3' ---TAAT
TA--- 5'
NNGTC--- 3'
3' ---CTGNN
NCAG--- 5'
3' ---CTTNN
NNAAG--- 5'
3' ---CCATG
G--- 5'
3' ---CCWG
G--- 5'
3' ---CCANTG
G--- 5'
3' ---GGATC
C--- 5'
NNGTC--- 3'
3' ---AAGC
TT--- 5'
3' ---GGTN
3' ---CTTAC
3' ---CTTAC
3' ---CTTAC
3' ---CTTAC
3' ---CTTAC
3' ---CTTAC
3' ---C
3' ---TCC
3' ---CTAG
3' ---CCN
3' ---CCNG
3' ---TCA
3' ---CYGC
NTGG--- 3'
NNNNACC--- 5'
--- 3'
GN--- 5'
--- 3'
GN--- 5'
--- 3'
GN--- 5'
--- 3'
GN--- 5'
--- 3'
GN--- 5'
--- 3'
GN--- 5'
C--- 3'
GCG--- 5'
CCT--- 3'
GGA--- 5'
GATC--- 3'
--- 5'
NCC--- 3'
NGG--- 5'
GNCC--- 3'
G--- 5'
ACT--- 3'
TGA--- 5'
CGYC--- 3'
RG--- 5'
BmcAI,
DpaI,
Bpa34I,
Eco255I, RflFII, ScaI, ZrmI
Acc113I,
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Msp17I
AsuI [58]
Anabaena subcylindrica
5' GGNCC
5' ---G
AsuII [59]
Anabaena subcylindrica
5' TTCGAA
5' ---TT
CGAA--- 3'
AsuIII
Anabaena subcylindrica
5' GRCGYC
5' ---GR
CGYC--- 3'
AsuC2I
Actinobacillus suis C2
5' CCSGG
5' ---CC
SGG--- 3'
AsuHPI [60]
Actinobacillus suis HP
5' GGTGA
5' ---GGTGAN6NN
AsuNHI [60]
Actinobacillus suis NH
5' GCTAGC
5' ---G
AtsI
5' GACNNNGTC
5' ---GACN
Anabaena variabilis
5' CYCGRG
5' ---C
YCGRG--- 3'
Anabaena variabilis
5' GGWCC
5' ---G
GWCC--- 3'
AvcI
Actinomyces violaceoniger
cristalomycini
5' GGNCC
5' ---G
GNCC--- 3'
AviII
5' TGCGCA
5' ---TGC
AvrII [18][26]
Anabaena variabilis UW
5' CCTAGG
5' ---C
CTAGG--- 3'
AvrBII
Arthrobacter variabilis
5' CCTAGG
5' ---C
CTAGG--- 3'
AxyI [63]
Acetobacter xylinus
5' CCTNAGG
5' ---CC
AvaI
[10][61][62]
AvaII [8][10]
[39][61][62]
3' CCNGG
3' AAGCTT
3' CYGCRG
3' GGSCC
3' CCACT
3' CGATCG
3' CTGNNNCAG
3' GRGCYC
3' CCWGG
3' CCNGG
3' ACGCGT
3' GGATCC
3' GGATCC
3' GGANTCC
3' ---CCNG
GNCC--- 3'
G--- 5'
3' ---AAGC
TT--- 5'
3' ---CYGC
RG--- 5'
3' ---GGS
CC--- 5'
3' ---CCACTN6N
3' ---CGATC
3' ---CTGNN
3' ---GRGCY
3' ---CCWG
3' ---CCNG
3' ---ACG
3' ---GGATC
3' ---GGATC
3' ---GGANT
--- 3'
N--- 5'
CTAGC--- 3'
G--- 5'
NNGTC--- 3'
NCAG--- 5'
C--- 5'
G--- 5'
G--- 5'
GCA--- 3'
CGT--- 5'
C--- 5'
C--- 5'
TNAGG--- 3'
CC--- 5'
Notes
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