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Keywords:
Sensory
Sulforaphane
Spectrum method
Descriptive analysis
Qualitative techniques
Strawberry
Emotions
a b s t r a c t
Sensory evaluation techniques are frequently used, however applied sensory is most often used within
private industry. Basic sensory techniques can be an invaluable aid to research on nutritional or functional
benets of natural products such as whole fruits, nuts and vegetables (through varietal selection, breeding,
etc.) in addition to clinical trials of botanicals. Products' sensory properties, including fruits and vegetables,
must be tailored to ultimately appeal to the consumer: no matter how healthy and nutritious a food is, if
it does not appeal to its intended end user, it is unlikely to succeed in today's marketplace. This paper outlines
the 5 S's or basic principles of applied sensory testing; Subjects, Site, Samples, Statistics, and Sensory Methods.
Two case studies are detailed where applied sensory is used to benec academic research; one as a clinical trial
of broccoli sprout extract, and the second as plant breeding research on strawberries. Finally, more in-depth
techniques are discussed so that one can ensure that product sensory properties are aligned with consumer expectations, in other words, that sensory congruence is achieved.
2012 Elsevier Inc. All rights reserved.
1. Introduction
With the rise in diet-related chronic disease in recent years, private industry and academic institutions alike have focused their
research on leveraging nutritional or functional benets of natural
products such as whole fruits, nuts and vegetables (through varietal
selection, breeding, etc.) as well as increasing the nutritional quality
of processed foods (through fortication, fat, sodium or sugar reduction initiatives, to cite only a few examples. As part of the effort to
discover, develop and market healthier products, understanding
nutritional and functional benets is key. Of additional importance
is highlighting and rening the products' sensory properties in an
attempt to ultimately appeal to consumers: no matter how healthy
and nutritious a food is, if it does not appeal to its intended end
user, it is unlikely to succeed in today's marketplace.
Used by many industries, sensory evaluation techniques have
been shown to be critical in the development, production and quality
maintenance of foodstuffs, personal care products, household goods,
textiles, pharmaceuticals and even furniture and automobile driving
experience. Sensory evaluation is dened as the scientic discipline
which encompasses all methods to evoke, measure, analyze and interpret human responses to the properties of foods and materials, as
perceived by the ve senses: taste, smell, touch, sight and hearing.
Specic senses of heightened interest are taste and smell, particularly
in their relationship with ingestive behavior. The application of
Corresponding author.
E-mail addresses: gvciville@sensoryspectrum.com (G.V. Civille),
koftedal@sensoryspectrum.com (K.N. Oftedal).
0031-9384/$ see front matter 2012 Elsevier Inc. All rights reserved.
doi:10.1016/j.physbeh.2012.04.015
G.V. Civille, K.N. Oftedal / Physiology & Behavior 107 (2012) 598605
599
600
G.V. Civille, K.N. Oftedal / Physiology & Behavior 107 (2012) 598605
water, mix coffee and water, heat ceramic cups, pour coffee, measure
temperature of coffee, cover coffee cup with watch glass to better
maintain temperature. Each step controls for variation in the nished
product. Usually instant coffee is sold with specic preparation
instructions, so it is assumed that even in-home it will be prepared
a certain way. Additionally, it is important to understand that interpretation is limited to the samples that were tested, how they were
prepared in the test. In the case of Domino's vs. Subway [7] a claim
was made by Domino's that their oven baked sandwiches were preferred to Subway by 2 to 1. The criticism of Domino's testing was
that they only tested ve of Subway's 16 sandwiches. Each of the 5
Subway sandwiches had a comparable Domino's Oven Baked sandwich. While Domino's asserted that they were testing the hot or
toasted sandwich line (Oven Baked vs. Subway Fresh Toasted), Subway allows consumers to toast any sandwich. Subway's position
was that a toasted version of every sandwich would be needed to
make a broad comparison of full product lines. The NAD concluded
that Domino's taste test was reasonable enough to allow for a more
narrow claim between the two hot sandwich lines (Domino's Oven
Baked vs. Subway Fresh Toasted), vs. all sandwiches.
In addition to preparation methods it is important to consider the
inherent properties of the samples, which will dictate the creation of
the descriptive lexicon or consumer questionnaire. In addition to attribute intensity, often samples differ in perception across time and
order. A more integrated approach is necessary to capture the sample
integrity, which relates to the whole impression of the product and
unity across components. Some examples of integrated attributes
are amplitude/harmony, balance, authenticity or delity and blend
[8]. Amplitude, as described by Thompson et al. [9], measures the balance and blendedness of avor components as well as the bloom and
impact throughout evaluation. The research group compared US ice
creams vs. Italian gelati and found that most of the Italian gelati had
higher amplitude ratings. Samples with higher amplitude also had
higher avor impact, longevity and bloom. The panelists had a minimum of 200 h evaluation experience. Panels that measure integrity
attributes need to have more experience than the minimum 100 h
of training for an expert panel [9].
The General Mills descriptive panel additionally measures complexity in products, [10]. The term used is harmonious, which captures the degree to which the aromatics make sense together and are
compliant. Sensory Spectrum primarily uses the term balance and
blend, which measures the degree to which the individual avor components (aromatics and tastes) blend together into a well-combined
sensory impression making it difcult to identify each component.
While basic tastes do not blend together as aromatics do, products
with high balance and blend tend to have a balanced taste prole, appropriate for that product category. One aspect not yet discussed is
that of avor appropriateness or delity. This answers the question,
is this what the product is supposed to be?, which requires product
concept knowledge. For example, if a avor is supposed to be mocha,
but has no coffee or espresso avor notes, it may be very harmonious
or high in balance and blend, however it will be low in delity.
Table 1
Tier 1 descriptive analysis proles from 5 dark rums.
SAMPLE
AROMATICS
Fruity
Toasted Grain
Sweet Aromatics Complex
Woody Complex
Black Pepper / Terpene
Brown Spice Complex
Aldehydic
Alcohol
Dark Rum Dark Rum Dark Rum Dark Rum Dark Rum
1
2
3
4
5
0.0
0.0
4.5
3.5
0.0
0.0
1.2
3.5
0.0
0.0
3.0
2.0
0.0
0.0
3.0
3.0
4.0
1.5
4.0
0.0
0.0
1.0
0.0
2.8
6.5
0.0
2.5
1.2
0.0
0.0
0.0
2.5
2.0
0.0
6.5
0.0
2.0
0.0
0.0
2.0
G.V. Civille, K.N. Oftedal / Physiology & Behavior 107 (2012) 598605
Table 2
Detailed descriptive analysis proles from 5 dark rums.
SAMPLE
Dark
Rum 1
Dark
Rum 2
Dark
Rum 3
Dark
Rum 4
Dark
Rum 5
AROMATICS
Fruity
Orange Peel
0.0
0.0
4.0
6.5
2.0
0.0
0.0
2.0
4.5
1.0
Banana
0.0
0.0
2.2
1.5
0.0
Apricot
0.0
0.0
0.0
0.0
1.0
Toasted Grain
0.0
0.0
1.5
0.0
0.0
4.5
3.0
4.0
2.5
6.5
Molasses
0.0
1.0
3.0
0.0
0.0
Caramelized
3.0
0.0
0.0
2.0
0.0
Buttery
1.5
0.0
0.0
0.0
0.0
Refiner's Syrup
0.0
2.0
1.0
0.0
1.5
Vanilla
0.0
0.0
0.0
0.0
5.0
Woody Complex
3.5
2.0
0.0
1.2
0.0
Dried Wood
0.0
2.0
0.0
0.0
0.0
Oaky
3.5
0.0
0.0
1.2
0.0
0.0
0.0
0.0
0.0
2.0
0.0
0.0
1.0
0.0
0.0
0.0
0.0
1.0
0.0
0.0
Aldehydic
1.2
3.0
0.0
0.0
0.0
Alcohol
3.5
3.0
2.8
2.5
2.0
Nutmeg
601
powdered BSE which was prepared according to Kensler et al., suspended in 4 oz of liquid [13]. Spectrum Descriptive Analysis was
used to prole fortied beverages.
First, the expert panel reviewed avor properties of GR and SF in
an aqueous solution. The SF was found to have a strong radish avor
and nasal pungency. Then, avor components were identied that
mask or match avor and chemical feeling characteristics of GR or
SF. Additionally, these avors needed to be common in the Chinese
diet and due to logistics, readily accessible as juices in the Qidong
region of The People's Republic of China. Since the SF extract was
anecdotally found by the study population to be more strongly
avored, more emphasis was placed on SF extracts. Flavor systems
identied by the panel as promising option to counteract the offnotes of BSE included tropical fruit avors (pineapple, lychee), citrus
avors, ginger and dairy avors (yogurt-type beverages such as
Yakult or Calpico). Table 1 contains the specic avors combination
tested. BSE was mixed into commercially available juices and beverages of the identied avors. Samples were evaluated by descriptive
analysis, using a consensus rating. Results for the broccoli and radish
complexes are shown below in Table 3.
Results showed that both pineapple-ginger and citrus pineapple
beverage systems meaningfully decreased the radish and broccoli
aroma and avor. The pineappleginger blend was particularly effective at reducing the radish aroma. However, ginger is known to contain
6-gingerol which is a bioactive compound with anti-carginogenic properties [14] and thus would confound the purported benets of GR and
SF. Therefore, citrus/pineapple avored beverage was identied as
being most promising without risk of confounding the benets of GR
and SF. The results showed the pineapple and citrus yogurt beverages
to reduce the broccoli avor of the GR extract to about threshold level
(0.8 on the 15 point Spectrum scale). The citrus pineapple blend was
most effective at additionally reducing radish avors in SF extracts.
Based on these ndings, a new trial was planned using citrus/pineapple
blend juice for delivery of SF and is currently taking place in China
(Table 4).
4.2. Case study 2: understand consumer perception of strawberry
varietals
As recommended by the USDA, fruits and vegetables are integral
to a healthy well-balanced diet, along with other natural products
such as whole grains, nuts and beans [15]. Those natural ingredients
constitute essential sources of vitamins, minerals, ber and other bioactive compounds that may help reduce the risk of some types of cancer and other chronic diseases. Growers and producers, however, are
facing many challenges balancing the nutritional quality of their
products with the inherent sensory variability of natural products,
meaning agricultural products can be variable from not only grower
to grower, but within packaged containers or items from the same
grower. Ultimately, understanding which sensory qualities appeal to
their customers is key to determine strategies to enhance the marketability and general consumption of their products.
Table 3
BSE beverage avors.
Code
Extract
P-GR
C-GR
CS- SF
CP-SF
PG-SF
CG-SF
PLG-SF
LG-SF
LG
W-SF
GR
GR
SF
SF
SF
SF
SF
SF
SF
Pineapple
Citrus yogurt beverage (Calpico)
Citrus yogurt beverage and soy
Citrus yogurt, pineapple
Pineapple Ginger
Citrus yogurt, ginger
Pineapple, lychee, ginger
Lychee, ginger
Lychee, ginger
Water
602
G.V. Civille, K.N. Oftedal / Physiology & Behavior 107 (2012) 598605
Table 4
Descriptive analysis data of BSE fortied beverages.
Aroma
Total aroma
Broccoli complex
Radish complex
Flavor
Total impact
Broccoli complex
Radish complex
a
Table 5
Consumers perception of strawberry avor and texture.
LG
WSF
PGR
CGR
CSSF
CPSF
PGSFa
CGSFa
PLGSFa
LGSFa
7
0
0
8
4.5
1.5
6
0.8
0
5.5
0
0
7
2.5
2.5
6.5
1.5
1.5
7.5
2
0
7.2
1
2.5
7.5
1
2
7.8
0.8
2.2
Consumer
preference
Most
107
preferred
Moderately 112
preferred
7
0
0
7
4.5
2.5
6.5
0.8
0
6
0.5
0
6.8
2
1.5
7
2
2
8
1.8
2
7.5
1.5
1.5
7.5
1.5
1.5
7.5
0.8
2
122
Contain ginger.
Least
105
preferred
108
130
Descriptive properties
Highest in fresh/raw
strawberry avor. Moderate
sweet and sour. High
juiciness and moist mass.
Moderate strawberry avor
but slight overripe/
fermented. Moderate
juiciness and seeds.
Moderate strawberry avor.
Moderate sweet and sour.
Moderate strawberry avor;
highest in ethyl maltol
(candy-like) with lower
fresh strawberry. Lowest in
hardness and denseness at
rst bite.
Lowest avor overall. Low
strawberry avor, highest in
green. Highest in denseness.
High uniformity of bite.
Lowest strawberry avor.
Slight green and overripe.
Phase 2. Understanding consumer, chefs and buyers attitudes, expectations and perceptions through qualitative discussions.
Qualitative research was conducted among consumers of strawberries. Since the consumers typically consumed strawberries as a
whole fruit, they discussed the appeal of strawberry characteristics
in that context. When eating strawberries as a plain whole fruit, the
characteristics that are most important to the consumers are color,
sweetness, juiciness and rmness of texture. Among the six strawberries tested Sample 107 was liked best, for its strawberry avor,
balance of sweet and sour, rmness and juiciness. Table 5 provides
a summary of the sensory characteristics of the samples tested with
consumers and their appeal to consumers.
Another group of interest included chefs and buyers. Due to timing
of the test, the strawberries evaluated descriptively were no longer
available and buyers were shown strawberries that were commercially
available at the time of study instead. Chefs and buyers had a different
perspective from the consumers with regards to strawberry use and
rather than highlighting the sensory benets of strawberry as a whole
fruit, they classied strawberries based on their function and potential
use. Fresher, juicier strawberries with a fully developed avor become
more versatile in their use, while those with underdeveloped avors
or overripe avor and texture have more restricted applications. Chefs
use appearance and tactile signals to judge the sensory characteristics
of strawberries. A selection of these sensory signals is shown in
Table 6. Sensory signals provide a basis for generating expectations
about the strawberry's avor and texture, as well as what stage of
shelf-life.
Outcomes of the research included an increased understanding of
the sensory signals associated with strawberries as well as identifying
Table 6
Sensory signals of strawberries and interpretation by chefs/buyers.
Sensory signal
Interpretation
Large size
Red all the way to the stem
Pale, white interior
G.V. Civille, K.N. Oftedal / Physiology & Behavior 107 (2012) 598605
Table 7
Themes and expectations associated with calming and energizing benets.
Calming
Familiarity vs. Discovery
603
Energizing
Familiarity/revisiting
The smell of your
own bed
Repetitive tasks or
motions
No surprise
Nostalgia and
traditions
Blend vs. contrast
Smoothness/blend
Vanilla
Smooth
Muted/deep colors
Singular vs. complex
Singularity
Single scent,
textures, sounds
Slow repetitive motion Slow repetitive
vs. intense activity
motion
Flowing, running
water (bath/lake)
Cream, lotion,
powder
Sleeping
Taking the time
Discovery
Smell and feel of a freshly made
bed
New task, spur of the moment
Surprise
Newness and search
Contrast/spike
Vanilla with spice or orange
Carbonated
Vibrant/bright colors
Complexity
Complex scents, textures,
sounds providing contrast
Intense activity
Waves and sprays (shower/
ocean)
Wash, scrubs, sprays
Awake
Rushing
the inherent variation present in the contemporary gene pool Consumer preferences were also obtained which will help to prioritize
fruit quality traits in strawberry breeding programs. The addition of
descriptive analysis to applied breeding programs will provide plant
breeders with unbiased tools to measure otherwise subjective fruit
quality traits. Furthermore, combining consumer preferences with
descriptive analysis is an approach that will result in the development
of consumer-driven improvements in not only strawberries but also
other nutritionally important agricultural commodities.
Another area of application of sensory methods is related to understanding consumer benet expectations and delivering against those
expectations. As seen from the Strawberry Case Study (Section 4.2) sensory signals can send different messages to consumers. Qualitative
methodologies can be used at the fuzzy front end or at very early
product development stages to understand which sensory properties
are inherently associated with specic benets in the mind of the consumer. Additionally, this information can be conrmed quantitatively
with large numbers of consumers (at least n = 150) for sound business
decision-making. Two examples show how more advanced sensory
methods can be used to achieve sensory congruence or harmony.
Example 1. In a study conducted with creative consumers, calming
and energizing benets of personal care products were investigated.
Qualitative methods used to generate a list of sensory expectations associated with the two benets included collages, interactions with a
wide variety of stimuli, compare and contrast exercises, etc. All of
those exercises were followed by a discussion to dive into the sensory
experience to be constructed around the benet to generate high alignment between expectations and actual product experience. Table 7
highlights the contrast in expectations that emerged from the discussion. Fig. 1 represents the sensory expectations congruent with the benet of calming. Such methods may help then better design a product
concept around the benet that is to be delivered by the product.
Example 2. A manufacturer of therapeutic products was struggling
with the avor of their current offering: consumers complaints about
the avor was high, the current avor was not liked with complaints
that it was too medicinal. In an effort to gain higher consumer acceptance and increase consumer satisfaction, the manufacturer decided to
reformulate and designed new prototypes with a completely different
604
G.V. Civille, K.N. Oftedal / Physiology & Behavior 107 (2012) 598605
Fig. 2. Map highlighting product similarities and differences from a descriptive and consumer standpoint.
avor system than that of its current products. In addition to the prototypes, it was decided that current products with a strong avor, current
products with a milder avor and mixture of strong current and prototype avors were to be included in the test.
Product properties were documented using descriptive analysis
and product acceptance and imagery was captured in a central location quantitative test, among consumers of the product category.
The data from both the descriptive panel and the quantitative consumer research was then mined for insights.
Using multivariate regression methods (partial least square regression), a map (Fig. 2) was generated that allowed to visualize
product similarities and differences both from a consumer and product understanding standpoint. As a note, this data has been modied
from its original form to conceal proprietary information and is used
for illustrative purposes only.
Not surprisingly, from a product standpoint, strong current products
clustered together, prototypes grouped together and mixture products
fell somewhere in the middle. From a consumer standpoint, it became
clear that prototypes were overall more liked than the strong-avored
current samples. However, when imagery was added to the map, current products were perceived as efcacious while the prototypes were
believed to be more natural yet less efcacious.
Based on these ndings, it was recommended that the manufacturer introduce on the market the new prototype under a different
brand than the current product and after product introduction, advertise the new product as being as efcacious as the current product
rather than replace the current product at the risk of creating consumer doubt in the efcacy of the new product.
6. Summary and conclusions
In conclusion, sensory evaluation can be an incredible asset in
health and wellness research on food and beverages, botanicals for
G.V. Civille, K.N. Oftedal / Physiology & Behavior 107 (2012) 598605
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