Table 1: Effect of pH on Activity of Enzyme

Time (min)
pH 2
pH 8
0
0.95
0.125
0.5
0.015
0.165
1
0.012
0.216
1.5
0.009
0.263
2
0.008
0.312
2.5
0.006
0.356
3
0.006
0.403
3.5
0.005
0.45
4
0.004
0.496
4.5
0.004
0.542
5
0.003
0.589

ALP
pH 10
0.075
0.08
0.085
0.091
0.096
0.102
0.108
0.113
0.119
0.125
0.13

Effect

f(x) = 0.0466636364

f(x) = - 0.04375454

f(x) = 0.0055727273

Effect of pH on Enzyme Controlled Reaction

pH 2
Linear (pH 2)
pH 8
f(x) = 0.0466636364x + 0.0761090909

Linear (pH 8)
pH 10
Linear (pH 10)

f(x) = - 0.0437545455x + 0.3554363636

f(x) = 0.0055727273x + 0.0687454545

This graph shows the trend that both pH 8 and pH 10 have similar
trends when it comes to graphs. They have a similar effect of pH because
they are both basic and they have in increase in absorption overtime.

Rate of reaction for each pH

pH 10
pH 8
pH 2

This bar graph shows that the reactions for pH 8 and pH 10 were most similar
from 2-10 min because they are basic, whereas there is a hint of a similarity in
each pH for the first minute of the reaction.

Table 2: Effect of Enzyme Concentration on Enzyme ALP

Time (min)
1a (Low)
2a (med) 3a (med-high) 4a (high)
0
0.093
3.61
0.21
0.217
1
0.109
0.402
0.36
0.447
2
0.121
0.44
0.453
0.555
3
0.133
0.48
0.54
0.699
4
0.145
0.519
0.635
0.767
5
0.156
0.56
0.723
0.873
6
0.168
0.598
0.811
0.979
7
0.18
0.637
0.897
1.086
8
0.19
0.678
0.986
1.192
9
0.202
0.72
1.068
1.293
10
0.213
0.761
1.153
1.395

Effect of Enzyme Concentration on Enzyme Controlled Reaction

1a (Low)
Linear (1a (Low))
2a (med)
Linear (2a (med))
3a (med-high)
Linear (3a (med-high))
4a (high)
f(x) = 0.1106454545x
++
0.2000363636
- 0.1078727273x
1.5022363636
f(x) = 0.0912363636x + 0.1649454545

Linear (4a (high))

f(x) = 0.0117818182x + 0.0847636364

This graph shows that the concentration has a great effect in the absorbance.
The solution with the higher concentration has a greater absorbance
than the solution with the lowest concentration.

d Reaction

1a (Low)

Linear (1a (Low))

2a (med)

Linear (2a (med))

3a (med-high)

Rate of Reaction for each Enzyme Concentration

4a (high)
3a (med-high)
2a (med)
1a (Low)

Linear (3a (med-high))

4a (high)

Linear (4a (high))

ct in the absorbance.
absorbance

This bar graph shows that from the time between 2-10 min, there
was a similarity in the rate of reactions for the different concentrations
whereas the first minute has a higher percentage of the medium
concentration for rate of reaction.

ntration

4a (high)
3a (med-high)
2a (med)
1a (Low)

oncentrations

Time (min)
0
1
2
3
4
5
6
7
8
9
10

1b (4C)
0.105
0.113
0.122
0.13
0.139
0.148
0.157
0.167
0.177
0.185
0.195

2b (20C)
0.082
0.093
0.104
0.114
0.124
0.134
0.143
0.154
0.163
0.172
0.182

3b (32C)
0.11
0.123
0.135
0.147
0.16
0.171
0.182
0.192
0.203
0.214
0.225

4b (60C)
0.302
0.395
0.456
0.509
0.56
0.608
0.653
0.696
0.74
0.781
0.821

Effect of Enzyme Temperature on Enzyme Controlled Reaction

f(x) = 0.0496181818x + 0.2951090909
1b (4C)
Linear (1b (4C))
2b (20C)
Linear (2b (20C))
3b (32C)
Linear (3b (32C))
4b (60C)
Linear (4b (60C))
f(x) = 0.0114090909x + 0.1008181818
f(x) =
= 0.0099272727x
0.0090454545x +
+ 0.0736181818
0.0946363636
f(x)

This graph shows that all the different temperatures reveal that
as the temperature increases, the absorbance increases as well.
In the end, the largest temperature, in this case 60C, had the
greatest increase in absorbance than all the other temperatures

led Reaction

Rate of reaction for each Enzyme Temperature

1b (4C)

4b (60C)

Linear (1b (4C))

3b (32C)

2b (20C)

2b (20C)

Linear (2b (20C))

1b (4C)

3b (32C)
Linear (3b (32C))
4b (60C)
Linear (4b (60C))

This graph reveals that all 4 temperatures had a similarity in

rate of reaction but the rate of reaction for the highest temperatur
was greater than compared to the increase in rate of
reaction for the other three temperatures.

mperature

4b (60C)
3b (32C)
2b (20C)
1b (4C)

ad a similarity in
he highest temperature