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Introduction
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Gametogenesis
o During this stage sperm and eggs develop their unique and distinctive structures
o Sperm
Round cells elongate until they become oval and pointed in one part
Lose their cytoplasm, retain only a little part
Form a flagellum for swimming (motility)
o Eggs
Do not transform much
Only increase in size
Have a large amount of yolk
Immotile
o Sperm and Eggs undergo meiotic divisions that reduce their chromosome number into HALF
SPERMATOGENESIS
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Stages of Development
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Spermatogonia
o Small, crowded and located at blunt end of testis
o Nuclei beaded appearance (chromatin)
o Large nuclei
o Mitotic divisions seen here
o Multiply first before undergoing maturation
Primary Spermatocytes
o Spermatogonium ready to divide
o Located in cysts near spermatogonia
o Very large nuclei
o Stages of prophase I identifiable in this specimen
Interphase
Inconspicuous appears finely granular single pale vascular nucleolus
Leptotene
Finely beaded filaments of chromatin
Chromatin becomes filamentous
Completely filled cell
Chromosomes longest here (UNCOILED)
Zygotene/Synaptene
Thickened regions in chromosomes
Beginning of pairing (synapsis) of homologous chromosomes
Synaptonemal complex
o Point of contact between 2 homologues
Paired chromosomes assumes shape of long loops
o Attached to extremity of nuclear envelop
Drawn to one side in a shape of a bouquet
Double thickness indicates advance pairing
Pachytene
Nuclear volume increases, chromosomes shorten and become thicker
Complete pairing and exchange of chromosome arms here
Cells in haploid condition
Chromatin stays in this condition for a long period
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STRUCTURES
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Seminiferous tubules
o Structures found in seminiferous t.
Small blood vesses filled with red-stained erythrocytes
Clumps of cells with conspicuous nuclei that contains blue-stained chromatin
Interstitial cells/Leydig cells
o Produce testosterone
Connective tissue cells with elongated nuclei
Sertoli Cells
o Nurse/sustentacular cells
o Spaced at regular intervals against basement membrane and around seminiferous tubules
Near spermatogonia
Have distinct outlines and large,oval
With indented nuclei with compound nucleoli
Surrounded by spermatozoa
Functions as:
Form blood-testes barrier
Nutrients and other substances do not directly reach cells
Determines which substances reach the germ cells
Spermatogonia are outside blood-testes barrier
Produce antigen-binding proteins
Necessary for spermiogenesis
o Two types of Sertoli Cells
Active Sertoli Cells
Normal function of supporting spermatozoa
Pyramidal shape with apex towards lumen and nucleus perpendicular to tubule
wall
Inactive (resting) Sertoli Cells
Polygonal in shape
Nucleus is parallel to wall of tubule
Spermatogenic Cells
o Arranged in a layer
o Vary in number with the phase of spermatogenic activity
Does not take place in simultaneously in all tubules
o The spermatogenic cells are:
Spermatogonia
Primary spermatocytes
Secondary spermatocytes
Spermatids
Spermatozoa
Spermatogonia
o Most primitive of the male sex cells
o Some undergo meiosis; progenies are translocated as they mature
Translocation mediated by sertoli cells
o Circumferential zones of more advanced cells inside zones of less advanced cells
o Criteria to identify spermatogonia
Rests on basement membrane and interrupted at intervals by sertoli cells
Gonial layer rapid mitosis (thin layers)
Nuclei is small, compared to spermatids
o 3 types of spermatogonia
1. TYPE A Spermatogonia
a. Chromatin appears fine and evenly dispersed
b. Single prominent chromatin nucleolus
c. Germ cell line (eccentric position where u see the chromatin nucleolus)
2. Type B Spermatogonia
a. Chromatin is coarse granules or flakes of heavily stained chromatin
b. Most common gonia
c. Last stage before they transform into primary spermatocytes
3. Intermediate Spermatogonia
a. Chromophilic chromatin in the form of delicate crusts or flakes attached
to membrane
b. Nucleoli in central position
Primary Spermatocytes
o Large size; large nuclei than the spermatogonia
o Visible chromosomes; nuclear membrane are disintegrated already
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Mature spermatozoa found in lumina of testis tubules and ducts is made up of:
Head (circle shaped and flattened)
Middle piece
Tail (long and flagellated)